CN217288473U - Reagent preset loop-mediated amplification centrifugal tube - Google Patents
Reagent preset loop-mediated amplification centrifugal tube Download PDFInfo
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- CN217288473U CN217288473U CN202220356188.3U CN202220356188U CN217288473U CN 217288473 U CN217288473 U CN 217288473U CN 202220356188 U CN202220356188 U CN 202220356188U CN 217288473 U CN217288473 U CN 217288473U
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Abstract
The utility model belongs to the technical field of utensil for loop-mediated isothermal amplification, concretely relates to reagent preset loop-mediated amplification centrifuging tube. The reagent preset loop-mediated amplification centrifuge tube comprises: the end surface of the pipe body is provided with a pipe body sealing groove; the cover body is provided with a cover body protruding part matched with the pipe body sealing groove on the covering surface; the enzyme accommodating cavity is formed on the covering surface of the cover body; a sample injection passage provided in the lid body; a channel sealing cover disposed in the sample injection channel. The centrifugal tube can inject reaction liquid required by loop-mediated isothermal amplification into the tube body during production, seals related enzyme wax in the enzyme containing cavity, seals the enzyme containing cavity with the sealing groove of the tube body through the protruding part of the cover body, and only needs to inject a sample through the sample liquid injection channel during detection.
Description
Technical Field
The utility model belongs to the technical field of utensil for loop-mediated isothermal amplification, concretely relates to reagent preset loop-mediated amplification centrifuging tube.
Background
The loop-mediated isothermal amplification technology is a new nucleic acid amplification method, which mainly depends on strand displacement DNA polymerase, 4 specific primers designed for 6 regions of target genes and proper reaction solution to realize exponential nucleic acid amplification under isothermal conditions (about 63 ℃).
The sensitivity of the loop-mediated isothermal amplification technology can be 10 times higher than that of Polymerase Chain Reaction (PCR), and theoretically, the method can be carried out only by adding the sample solution and each reactant into a test tube and placing the test tube into a piece of constant temperature equipment. However, due to the extremely high sensitivity, when the cleanliness of the environment is insufficient, contaminants are easily mixed in the process of adding the reagent into the reaction vessel, false positive occurs, and the advantages of simple equipment and rapid amplification required by the loop-mediated isothermal amplification technology cannot be fully exerted.
SUMMERY OF THE UTILITY MODEL
The utility model aims at providing a reagent preset loop-mediated amplification centrifuging tube to solve the technical problem that the reagent needs to be added into a reaction container when the loop-mediated isothermal amplification is carried out at present, and pollutants are easily mixed in the process.
In order to solve the above technical problem, the utility model provides a reagent preset loop-mediated amplification centrifuge tube, include: a pipe body sealing groove is formed in the end face of the pipe body; the cover body is provided with a cover body protruding part matched with the pipe body sealing groove on the covering surface; the enzyme accommodating cavity is formed on the covering surface of the cover body; a sample injection passage provided in the lid body; a channel sealing cover disposed in the sample injection channel.
Furthermore, two circles of pipe body sealing grooves are formed in the end face of the pipe body; the cover body and the cover combination surface of the pipe body are provided with two cover body protruding parts.
Furthermore, the inserting end of the convex part of the cover body is fixedly connected with a cover body sealing ring.
Furthermore, a cover body sealing groove is formed between the two cover body protruding parts; a pipe body protruding part matched with the sealing groove of the cover body is arranged between the two pipe body sealing grooves; and a pipe body sealing ring is fixedly connected to the insertion end of the pipe body protruding part.
Furthermore, a wax-sealed coagulating groove is formed on the wall of the enzyme accommodating cavity.
Further, the sample injection channel includes: an injection section; a sealing section having a diameter larger than the injection section to form a step; the rubber plug buckling groove is arranged on the ladder surface of the ladder to buckle the rubber plug.
The beneficial effects of the utility model are that, the utility model discloses aim at solving and need add reaction vessel with reagent when carrying out loop-mediated isothermal amplification at present, and the technical problem of pollutant is easily sneaked into to this process, through this reagent preset loop-mediated amplification centrifuging tube, can pack the required reaction liquid injection body of loop-mediated isothermal amplification when production, hold the chamber with relevant enzyme wax seal at the enzyme to seal through lid bulge and body seal groove, only need when detecting with the sample through the injection of sample liquid injection passage can.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for the embodiments or the prior descriptions will be briefly introduced below, and it is obvious that the drawings in the following description are some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to these drawings without creative efforts.
FIG. 1 is an exploded view of a reagent-preset loop-mediated amplification centrifuge tube according to the present invention;
FIG. 2 is a cross-sectional view of a reagent-preset loop-mediated amplification centrifuge tube of the present invention;
in the figure:
a pipe body 100, a pipe body seal groove 110, a pipe body protrusion 120, a pipe body seal ring 130;
a cover body 200, a cover body protrusion 210, a cover body seal ring 220, and a cover body seal groove 230;
an enzyme accommodating chamber 300, a wax-sealed coagulation tank 310;
a sample injection channel 400, an injection section 410, a sealing section 420, a rubber plug catching groove 430 and a rubber plug 440;
the channel seals the cover 500.
Detailed Description
To make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions of the present invention will be described clearly and completely with reference to the accompanying drawings, and obviously, the described embodiments are some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments of the ordinary skilled person in the art without creative work belong to the protection scope of the present invention.
Examples
As shown in fig. 1 and fig. 2, the present invention provides a reagent preset loop-mediated amplification centrifuge tube, comprising: a pipe body 100 having a pipe body sealing groove 110 formed on an end surface thereof; a cover body 200 having a cover body protrusion 210 adapted to the pipe body sealing groove 110 on a cover surface thereof; an enzyme accommodating chamber 300 opened on the covering surface of the cover body 200; a sample injection passage 400 provided in the lid body 200; a channel sealing cap 500 disposed in the sample injection channel 400.
Through this reagent preset formula loop-mediated amplification centrifuging tube, can inject the required reaction liquid of loop-mediated isothermal amplification into body 100 when production, hold chamber 300 with relevant enzyme wax seal simultaneously to seal through lid bulge 210 and body seal groove 110, be about to the centrifuging tube as reaction vessel, only need open the sealed lid of passageway 500 after inject the sample through appearance liquid injection channel 400 when detecting can, can greatly reduce the inside risk of pollutant sneaking into the centrifuging tube.
As shown in fig. 2, two rings of the pipe body sealing groove 110 may be formed on the end surface of the pipe body 100; two cover protruding parts 210 can be arranged on the covering surface of the cover 200 and the tube 100; the two cover protrusions 210 and the two circles of pipe body sealing grooves 110 form a labyrinth seal after being covered, so that external pollutants are prevented from entering the centrifugal pipe along with air.
As shown in fig. 2, a cover sealing ring 220 may be fixedly coupled to the insertion end of the cover protrusion 210 to clamp the cover protrusion 210 into the corresponding pipe body sealing groove 110, so as to improve the sealing performance.
In this embodiment, a cover sealing groove 230 may be formed between the two cover protrusions 210; a pipe body protrusion 120 adapted to the cover body sealing groove 230 is disposed between the two pipe body sealing grooves 110; a tube sealing ring 130 is fixedly connected to the insertion end of the tube protruding part 120; thereby further improving the sealability.
As shown in fig. 2, a wax-sealed coagulation groove 310 may be formed on a wall of the enzyme accommodating chamber 300, so that after the wax used for encapsulating the enzyme is solidified, the wax-sealed coagulation groove 310 is clamped to prevent the wax-sealed coagulation groove from falling off.
As shown in fig. 2, the sample solution injection channel 400 may include: an injection section 410; a sealing section 420 having a diameter larger than that of the injection section 410 to form a step; a rubber plug catching groove 430 opened on the step surface of the step to catch the rubber plug 440. In this embodiment, the channel sealing cap 500 may be threadedly engaged with the upper portion of the sealing section 420 as a first seal of the entire liquid injection channel 400, and the plug 440 may be snapped into the plug snap-in groove 430 as a second seal of the entire liquid injection channel 400, thereby preventing contaminants from entering the centrifuge tube through the injection section 410.
By the reagent preset type loop-mediated amplification centrifuge tube, reaction liquid required by loop-mediated isothermal amplification can be injected into the tube body 100 in production, for example, in a workshop with higher cleanliness, related enzyme wax is sealed in the enzyme containing cavity 300, and then sealing is completed by matching the cover body protrusion 210 with the tube body sealing groove 110, matching the rubber plug 440 with the rubber plug catching groove 430, and matching the channel sealing cover 500 with the sealing section 420; when detection is needed, after the channel sealing cover 500 is opened, the syringe is used for penetrating the rubber plug 440, a sample is injected into the tube body 100, and then the channel sealing cover 500 is screwed back; then, the temperature is maintained, and after the liquid in the tube body 100 is heated, the centrifugal tube is inverted to melt the wax in the enzyme containing cavity 300, and the enzyme is released, so that the amplification is performed.
To sum up, the utility model provides a reagent preset formula loop-mediated isothermal amplification centrifuging tube can hold the chamber at the enzyme with relevant enzyme wax seal the required reaction liquid injection body of loop-mediated isothermal amplification when production to seal through lid bulge and body seal groove, when detecting only need with the sample through appearance liquid injection passage inject into can, can greatly reduce the pollutant sneak into the inside risk of centrifuging tube.
In the description of the present invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and simplification of description, but do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus, should not be construed as limiting the present invention. Furthermore, the terms "first," "second," and "third" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.
In light of the foregoing description of the preferred embodiments of the present invention, it is to be understood that various changes and modifications may be made by those skilled in the art without departing from the spirit and scope of the invention. The technical scope of the present invention is not limited to the content of the specification, and must be determined according to the scope of the claims.
Claims (6)
1. A reagent preset loop-mediated amplification centrifuge tube, comprising:
a pipe body (100) with a pipe body sealing groove (110) on the end surface;
a cover body (200) which is provided with a cover body protruding part (210) matched with the pipe body sealing groove (110) on the covering surface;
an enzyme accommodating chamber (300) which is opened on the covering surface of the cover body (200);
a sample injection channel (400) opened in the lid body (200);
a channel sealing cap (500) disposed within the sample injection channel (400).
2. The reagent-preset loop-mediated amplification centrifuge tube of claim 1,
the end surface of the pipe body (100) is provided with two circles of pipe body sealing grooves (110);
the cover body (200) and the cover combining surface of the pipe body (100) are provided with two cover body protruding parts (210).
3. The reagent-preset loop-mediated amplification centrifuge tube of claim 2,
and a cover body sealing ring (220) is fixedly connected to the insertion end of the cover body bulge (210).
4. The reagent-preset loop-mediated amplification centrifuge tube of claim 2,
a cover body sealing groove (230) is formed between the two cover body convex parts (210);
a pipe body protruding part (120) matched with the cover body sealing groove (230) is arranged between the two pipe body sealing grooves (110);
and a pipe body sealing ring (130) is fixedly connected to the insertion end of the pipe body protruding part (120).
5. The reagent-preset loop-mediated amplification centrifuge tube of claim 1,
the wall of the enzyme accommodating cavity (300) is provided with a wax-sealed coagulating groove (310).
6. The reagent-preset loop-mediated amplification centrifuge tube of claim 1,
the sample injection channel (400) includes:
an injection section (410);
a sealing section (420) having a diameter larger than the injection section (410) to form a step;
a rubber plug catching groove (430) which is arranged on the ladder surface of the ladder to catch the rubber plug (440).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202220356188.3U CN217288473U (en) | 2022-02-22 | 2022-02-22 | Reagent preset loop-mediated amplification centrifugal tube |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202220356188.3U CN217288473U (en) | 2022-02-22 | 2022-02-22 | Reagent preset loop-mediated amplification centrifugal tube |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN217288473U true CN217288473U (en) | 2022-08-26 |
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ID=82929696
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202220356188.3U Active CN217288473U (en) | 2022-02-22 | 2022-02-22 | Reagent preset loop-mediated amplification centrifugal tube |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN217288473U (en) |
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2022
- 2022-02-22 CN CN202220356188.3U patent/CN217288473U/en active Active
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