CN217628371U

CN217628371U - Detection tube and detection device

Info

Publication number: CN217628371U
Application number: CN202220796512.3U
Authority: CN
Inventors: 王战会; 张彦杰; 王树相
Original assignee: TIANJIN MNCHIP TECHNOLOGIES CO LTD
Current assignee: Tianjin Weinanxin Technology Co ltd
Priority date: 2022-03-30
Filing date: 2022-03-30
Publication date: 2022-10-21
Anticipated expiration: 2032-03-30

Abstract

The utility model provides a detection tube is applicable to biological detection technical field for bear reaction liquid in the testing process that has the evaporation material to produce. This detection tube includes: the bottom of the pipe body is closed, and the top of the pipe body is provided with an opening; and the plugging part can be inserted in the tube body, and when the detection tube is in a detection state, the distance between the bottom end of the plugging part and the lowest point of the bottom of the tube body is greater than or equal to the distance between the liquid level position of the reaction liquid and the lowest point of the bottom of the tube body. The setting of shutoff portion not only reduces the evaporation of evaporation material but occupation space, shortens evaporation condensation route moreover for even the evaporation, also can be very fast during the condensation drips back reaction liquid, thereby do not influence reaction liquid concentration, and then guarantee to detect the accuracy.

Description

Detection tube and detection device

Technical Field

The utility model relates to a biological detection technology field, concretely relates to detecting tube and detection device.

Background

Detection such as fluorescence quantitative Polymerase Chain Reaction (PCR) and the like has important significance for biological research and medical clinical diagnosis. In the detection operation processes of PCR and the like, the reagent is concentrated at the bottom of the detection tube, the distance between the reagent and the tube cover is far away, and in the reaction temperature rise process, evaporated substances are generated and are condensed on the inner wall of the tube cover and the side wall of the tube, so that the concentration of the reagent is influenced, and the accuracy of a detection result is reduced.

SUMMERY OF THE UTILITY MODEL

In view of this, the embodiment of the utility model provides a detection tube and detection device to reagent evaporation influences reagent concentration in solving prior art in the detection tube, thereby reduces the technical problem who detects the accuracy.

According to an aspect of the present invention, an embodiment of the present invention provides a detecting tube for carrying reaction liquid in a detecting process of the generation of evaporated substances. The detection tube includes: the bottom of the pipe body is closed, and the top of the pipe body is provided with an opening; and the plugging part can be inserted into the pipe body, and when the detection pipe is in a detection state, the distance between the bottom end of the plugging part and the lowest point of the bottom of the pipe body is greater than or equal to the distance between the liquid level position of the reaction liquid and the lowest point of the bottom of the pipe body.

In one embodiment, the detection tube further comprises a tube cover, which is adapted to the opening and can cover the opening.

In one embodiment, the top end of the blocking portion is integrally connected with the tube cover.

In one embodiment, the plug is of the same material as the tube cap.

In one embodiment, the blocking portion and the tube cover are of an integral structure prepared by an integral molding method.

In one embodiment, the liquid level position of the reaction liquid is determined based on the volume of the sensing tube and a preset sensing volume corresponding to the volume of the sensing tube.

In one embodiment, the tube body comprises a conical liquid carrying part and a cylindrical transition part which are arranged in sequence from the bottom of the tube body to the top of the tube body, or the tube body comprises a semicircular liquid carrying part and a cylindrical transition part which are arranged in sequence from the bottom of the tube body to the top of the tube body.

In one embodiment, the blocking portion is a solid structure, or the blocking portion is a structure having a hollow cavity.

In one embodiment, the top end of the blocking portion has a lower groove.

According to another aspect of the present invention, an embodiment of the present invention provides a detection device for detecting a reaction liquid generated by a volatile substance, including the detection tube of any of the above embodiments.

The embodiment of the utility model provides a detecting tube for bear reaction liquid in the testing process that has the evaporation material to produce. The detection tube is provided with a plugging part which can be inserted in the tube body, and when the detection tube is in a detection state, the distance between the bottom end of the plugging part and the lowest point of the bottom of the tube body is greater than or equal to the distance between the liquid level position of the reaction liquid and the lowest point of the bottom of the tube body. The setting of shutoff portion not only reduces the evaporation of evaporation material but occupation space, shortens evaporation condensation route moreover for even the evaporation, also can condense fast and drip back to reaction liquid in, thus do not influence reaction liquid concentration, and then guarantee to detect the accuracy.

Drawings

Fig. 1 is a schematic structural view of a detection tube according to an embodiment of the present invention.

Fig. 2 is an exploded view of the structure of the detector tube provided in the embodiment shown in fig. 1.

Fig. 3 is a schematic structural view of a detection tube according to an embodiment of the present invention.

Fig. 4 is a schematic structural diagram of a detection tube according to an embodiment of the present invention.

Fig. 4a is a schematic structural diagram of a detection tube according to an embodiment of the present invention.

Fig. 4b is a schematic structural diagram of a detection tube according to an embodiment of the present invention.

Fig. 5 is a schematic structural view of a detection tube according to an embodiment of the present invention.

Fig. 6 is an exploded view of the structure of the detector tube provided in the embodiment shown in fig. 5.

Fig. 7a is a schematic structural diagram of a tube according to an embodiment of the present invention.

Fig. 7b is a schematic structural diagram of a tube according to an embodiment of the present invention.

Fig. 8a is a schematic structural view of a detection tube according to an embodiment of the present invention.

Fig. 8b is a schematic structural view of a detection tube according to an embodiment of the present invention.

Fig. 8c is a schematic structural diagram of a detection tube according to an embodiment of the present invention.

Fig. 9a is a schematic structural view of a detection tube according to an embodiment of the present invention.

Fig. 9b is a schematic structural view of a detection tube according to an embodiment of the present invention.

Fig. 9c is a schematic structural diagram of a detection tube according to an embodiment of the present invention.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.

The PCR technique is a molecular biological technique for amplifying a specific Deoxyribonucleic Acid (DNA) fragment. The fluorescence quantitative PCR technology is that a fluorescent group is added into a PCR reaction system, and the content of target DNA in a sample is obtained by collecting the fluorescence signal intensity of the sample in the amplification process. The fluorescent quantitative PCR technology realizes rapid and large-scale amplification and detection of trace target DNA fragments in a sample, and is of great importance in the processes of biological research and medical clinical diagnosis.

Taking PCR detection as an example, during the PCR detection process, evaporation substances are generated, and the evaporation of the reagent affects the concentration of the reagent, thereby reducing the detection accuracy. In the prior art, paraffin is added in the detection tube or a heatable tube cover is arranged for the detection tube so as to reduce reagent evaporation. However, paraffin affects the PCR reaction, and the provision of a heat pipe cover makes the detection apparatus more complicated and increases the cost. Therefore, the technical problem that the evaporation influence of the reagent in the detection tube reduces the detection accuracy is not solved on the premise of ensuring reliability and economy in the prior art.

In order to solve the problem, the utility model provides a detection tube sets up the shutoff portion that can insert and establish in the body. When the detection tube is in a detection state, the distance between the bottom end of the plugging part and the lowest point of the bottom of the tube body is greater than or equal to the distance between the liquid level position of the reaction liquid and the lowest point of the bottom of the tube body. The setting of shutoff portion not only reduces the evaporation of evaporation material but occupation space, shortens evaporation condensation route moreover for even produce the evaporation, also can condense soon and drip back to reaction liquid in, thereby do not influence reaction liquid concentration, and then guarantee to detect the accuracy.

The present invention will be further illustrated with reference to fig. 1 to 9 c.

It should be noted that the embodiment of the present invention provides a detecting tube for carrying a reaction liquid during a detection process in which an evaporated substance is generated. The embodiment of the utility model provides a detecting tube is applicable to including, but not limited to bear reaction solution in the PCR detects.

Fig. 1 is a schematic structural view of a detection tube according to an embodiment of the present invention. Fig. 2 is an exploded view of the structure of the detector tube provided in the embodiment shown in fig. 1. Fig. 3 is a schematic structural view of a detection tube according to an embodiment of the present invention. As shown in fig. 1, 2 and 3, the detecting tube includes: a tube body 1 and a sealing part 2. The bottom of body 1 seals and the top of body 1 is equipped with the opening, and shutoff portion 2 can insert and establish in body 1, and when the detection tube was in the detection state, the distance between the bottom of shutoff portion 2 and the bottom minimum of body 1 was greater than or equal to the distance between the liquid level position of reaction liquid and the bottom minimum of body 1.

Illustratively, the bottom end of the plugging portion 2 is close to or in contact with the liquid surface of the reaction liquid.

Specifically, a distance h2 between the bottom end of the stopper portion 2 and the lowest bottom point of the tube body 1 is greater than or equal to a distance h1 between the liquid level position of the reaction liquid and the lowest bottom point of the tube body 1, that is, the bottom end of the stopper portion 2 is close to or in contact with the liquid level of the reaction liquid. As shown in fig. 3, the bottom end of the plugging portion 2 is close to the liquid surface of the reaction liquid, the bottom end of the plugging portion 2 has a small distance from the liquid surface, the evaporation space for the evaporation substance is small, and even if evaporation occurs, the evaporation substance can be quickly condensed and dripped back into the reaction liquid. As shown in fig. 1, the bottom end of the plugging portion 2 contacts the liquid level of the reaction liquid, and the bottom end of the plugging portion 2 directly contacts the liquid level, so that space occupied by evaporation of the evaporation substances is not provided, and evaporation is avoided.

The embodiment of the utility model provides an in, the test tube is provided with can insert the shutoff portion 2 of establishing in body 1, and when the test tube was in the detection state, the bottom of shutoff portion 2 was close to or contacted the liquid level. The setting of shutoff portion 2 not only reduces the evaporation of evaporation material but occupation space, shortens the evaporation condensation route moreover for even the evaporation, also can condense soon and drip back to reaction liquid in, thus do not influence reaction liquid concentration, and then guarantee to detect the accuracy.

In an alternative embodiment, the outer wall of the closure portion 2 may be in slotted contact with the inner wall of the tubular body 1. As shown in fig. 2 and fig. 1, although there is a gap between the outer wall of the blocking portion 2 and the inner wall of the tube body 1, the blocking portion 2 can be inserted into the tube body 1 and clamped in the tube body 1, and the clamped blocking portion 2 reduces the space occupied by evaporation of the volatile substance.

In an alternative embodiment, the outer part of the closing off part 2 may also be in full intimate contact with the inner wall of the tube body 1 (not shown in the figures). The outer wall of shutoff portion 2 and the inner wall contact of body 1 for form complete confined space between the bottom of shutoff portion 2 and the bottom of body 1, confined space not only reduces the evaporation of evaporation material but occupation space, shortens evaporation condensation route moreover, even make even the evaporation, also can condense the drippage soon and fall back to in the reaction liquid.

Fig. 4 is a schematic structural view of a detection tube according to an embodiment of the present invention. As shown in fig. 4, the detection tube further includes a tube cover 3. The tube cover 3 is fitted to the opening and can cover the opening. When the detection tube is in the detection state, the bottom of shutoff portion 2 with be close to or contact the liquid level, the top and the tube cap 3 adaptation of shutoff portion 2 for tube cap 3 can the gland on the top of shutoff portion 2, makes shutoff portion 2 be difficult for shifting, further makes to form sealed space more sealed between the bottom of shutoff portion 2 and the bottom of body 1, but further provides safeguard for reducing the evaporation occupation space.

The tip of the plugging portion 2 and the tube cap 3 may be connected together or separated from each other, as long as the tip of the plugging portion 2 is fitted to the tube cap 3 and the tube cap 3 can be pressed against the tip of the plugging portion 2.

It should be noted that the tube body 1 and the tube cap 3 may be connected together (i.e., fixedly connected), and the tube body 1 and the tube cap 3 may be separated (i.e., detachably connected). Fig. 4a is a schematic structural diagram of a detection tube according to an embodiment of the present invention. Fig. 4b is a schematic structural diagram of a detection tube according to an embodiment of the present invention. As shown in fig. 4b, when the tube body 1 and the tube cover 3 are fixedly connected, they can be connected by a bending portion, and the tube cover 3 is turned over by the bending portion so as to cover the opening of the tube body 1. As shown in fig. 4a, when the tube body 1 and the tube cap 3 are detachably connected, the tube cap 3 can be directly pressed on the opening of the tube body 1, or can be connected by a screw (not shown), or can be connected by a sealing safety buckle (not shown).

The material of the plugging portion 2 may be Polyethylene (PE), polycarbonate (PC), polypropylene (PP), or the like, and the material of the plugging portion 2 may be an inert substance that does not chemically react with the reaction liquid.

Furthermore, the embodiment of the utility model provides a detection tube compares with adding paraffin among the prior art in the detection, and shutoff portion 2 does not influence the PCR reaction, improves the reliability of detection tube, compares with setting up heating tube lid 3 among the prior art, and preparation simple process, the cost is lower, saves financial resources.

In an alternative embodiment, as shown in fig. 4, 4a and 4b, the top end of the blocking portion 2 and the tube cover 3 are two separate bodies and are not integrally connected.

In another alternative embodiment, the top end of the blocking portion 2 is integrally connected with the tube cover 3. Fig. 5 is a schematic structural diagram of a detection tube according to an embodiment of the present invention, and fig. 6 is an exploded structural diagram of the detection tube according to the embodiment shown in fig. 5. As shown in fig. 5 and 6, the top end of the blocking portion 2 is connected with the tube cap 3 into a whole, when the detection tube is in a detection state, the distance between the bottom end of the blocking portion 2 and the bottom end of the tube body 1 is relatively fixed, so that the probability of slippage of the blocking portion 2 on the tube body 1 is reduced, and the detection tube is more convenient to use.

In one embodiment, the plugging portion 2 and the tube cover 3 are made of the same material, and the same material facilitates integral preparation.

In a further embodiment, the blocking portion 2 and the tube cap 3 are an integral structure manufactured by an integral molding method, which is convenient for mechanized mass production.

Specifically, the length of the stopper 2 is correlated with the liquid level position of the detection tube, and the liquid level position of the reaction liquid is determined based on the volume of the detection tube and a preset detection volume corresponding to the volume of the detection tube.

For example: the position of the liquid surface of the reaction liquid can be determined and the length of the stopper portion 2 can be determined by setting the volume of the detection tube to 50. Mu.L, setting the preset detection volume of the detection tube to 10. Mu.L, and setting the volume of the detection tube to 50. Mu.L.

In one embodiment, the volume of the detection tube is 20 μ L, and the preset detection volume of the detection tube is 5 μ L.

In another embodiment, the volume of the detection tube is 10 μ L, and the preset detection volume of the detection tube is 2 μ L.

Fig. 7a is a schematic structural diagram of a tube according to an embodiment of the present invention. As shown in fig. 7a, the tubular body 1 comprises a conical liquid-carrying portion 12 and a cylindrical transition portion 11 arranged in this order from the bottom of the tubular body 1 to the top of the tubular body 1.

Fig. 7b is a schematic structural diagram of a tube according to an embodiment of the present invention. As shown in fig. 7b, the tubular body 1 comprises a semicircular liquid-carrying portion 13 and a cylindrical transition portion 11 which are sequentially arranged from the bottom of the tubular body 1 to the top of the tubular body 1.

The embodiment of the utility model provides an in, provide the body of different shapes, make the detection tube shape more diversified, be suitable for different detection scenes.

In one embodiment, as shown in connection with fig. 1, the blocking portion 2 is a solid structure.

Fig. 8a is a schematic structural view of a detection tube according to an embodiment of the present invention. Fig. 8b is a schematic structural view of a detection tube according to an embodiment of the present invention. Fig. 8c is a schematic structural diagram of a detection tube according to an embodiment of the present invention. As shown in fig. 8a, 8b and 8c, the closing off part 2 is a structure 4 with hollow chambers. The hollow cavity 4 is formed in the blocking part 2, so that materials can be saved, the preparation cost is reduced, the excessive expansion force generated by heating the blocking part 2 can be reduced, and the stable operation of detection is ensured.

In a further embodiment, the volume ratio of the hollow chamber 4 to the closing off part 2 is between 0.2 and 0.6.

It should be noted that the hollow chamber 4 has a structure including, but not limited to, a rectangle, a square, a cylinder, etc.

Fig. 9a is a schematic structural view of a detection tube according to an embodiment of the present invention. Fig. 9b is a schematic structural view of a detection tube according to an embodiment of the present invention. Fig. 9c is a schematic structural view of a detection tube according to an embodiment of the present invention. As shown in fig. 9a, 9b and 9c, the top end of the blocking part 2 is provided with the lower groove 5, and the design of the lower groove 5 saves more material and cost during preparation.

When the tip of the closing part 2 and the cap 3 are joined together, the lower groove 5 is provided at the top of the whole formed by joining the closing part 2 and the cap 3, as shown in fig. 9 c.

The embodiment of the utility model provides an in, be provided with in detection device's the test tube and insert the shutoff portion 2 of establishing in body 1, at detection device from the bottom heating during operation of test tube, the setting of shutoff portion not only reduces the evaporation of evaporation material but occupation space, shortens evaporation condensation route moreover for even evaporation, also can condense soon and drip back to reaction liquid in, from not influencing reaction liquid concentration, and then guarantee to detect the accuracy.

It should be noted that the detection device includes, but is not limited to, a PCR detector.

It is to be understood that, as used herein, the term "includes" and variations thereof are open-ended, i.e., "including but not limited to". The term "one embodiment" means "at least one embodiment"; the term "another embodiment" means "at least one additional embodiment". In this specification, the schematic representations of the terms used above are not necessarily intended to refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples. Furthermore, various embodiments or examples and features of different embodiments or examples described in this specification can be combined and combined by one skilled in the art without contradiction.

In the present invention, unless otherwise explicitly specified or limited, the terms "mounted," "connected," "secured," and the like are to be construed broadly, e.g., as being fixedly connected, detachably connected, or integrated; may be mechanically coupled, may be electrically coupled or may be in communication with each other; they may be directly connected or indirectly connected through intervening media, or they may be connected internally or in any other suitable relationship, unless expressly stated otherwise. The specific meaning of the above terms in the present invention can be understood according to specific situations by those skilled in the art.

The above description is only a preferred embodiment of the present invention, and should not be taken as limiting the invention, and any modifications, equivalents and the like made within the spirit and principle of the present invention should be included in the scope of the present invention.

Claims

1. A detector tube for carrying a reactive liquid during detection of the production of a volatile substance, the detector tube comprising:

the bottom of the pipe body is closed, and the top of the pipe body is provided with an opening;

and the plugging part can be inserted into the tube body, and when the detection tube is in a detection state, the distance between the bottom end of the plugging part and the lowest point of the bottom of the tube body is greater than or equal to the distance between the liquid level position of the reaction liquid and the lowest point of the bottom of the tube body.

2. The detector tube according to claim 1, further comprising a tube cover adapted to fit over the opening and capable of covering the opening.

3. The detection tube according to claim 2, wherein a tip end of the plugging portion is integrally connected to the tube cover.

4. The detector tube of claim 3, wherein the blocking portion is the same material as the tube cap.

5. The detection tube according to claim 4, wherein the blocking portion and the tube cover are of an integral structure prepared by an integral molding method.

6. The detection tube according to any one of claims 1 to 4, wherein the liquid level position of the reaction liquid is determined based on a volume of the detection tube and a preset detection volume corresponding to the volume of the detection tube.

7. The detection tube according to any one of claims 1 to 4,

the tube body comprises a conical liquid carrying part and a cylindrical transition part which are arranged from the bottom of the tube body to the top of the tube body in sequence, or,

the body includes from the bottom of body to the semi-circular liquid-carrying portion and the cylindrical transition portion that set gradually of top of body.

8. The detection tube according to any one of claims 1 to 4, wherein the blocking portion is of a solid structure, or a structure having a hollow chamber.

9. The detector tube according to any one of claims 1 to 4, wherein the tip of the blocking portion has a lower groove.

10. A detecting unit for detecting a reaction liquid generated by an evaporated substance, comprising the detecting tube according to any one of claims 1 to 9.