CN217212408U - Reactor and reaction detection system - Google Patents
Reactor and reaction detection system Download PDFInfo
- Publication number
- CN217212408U CN217212408U CN202221633034.0U CN202221633034U CN217212408U CN 217212408 U CN217212408 U CN 217212408U CN 202221633034 U CN202221633034 U CN 202221633034U CN 217212408 U CN217212408 U CN 217212408U
- Authority
- CN
- China
- Prior art keywords
- liquid
- cavity
- reaction cavity
- reaction
- overflow
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Landscapes
- Automatic Analysis And Handling Materials Therefor (AREA)
Abstract
The application provides a reactor and reaction detection system. The reactor comprises: the device comprises a main body, a reaction cavity, an overflow pipe, a liquid injection pipe and an emptying pipe, wherein the reaction cavity is opened at the top part; the detector is arranged in the mounting hole and extends into the reaction cavity; and the stirrer extends into the reaction cavity. According to the reactor provided by the application, the overflow pipe, the liquid injection pipe and the emptying pipe which are communicated with the reaction cavity are arranged on the main body, so that a substrate liquid can be directly injected through the liquid injection pipe to clean the reaction cavity, and the substrate liquid is discharged through the emptying pipe after being cleaned, so that the cleaning efficiency is high; the overflow pipe is arranged, so that the substrate liquid can be conveniently and quickly quantified when being injected into the reaction cavity, and the stirrer is arranged to stir the liquid in the reaction cavity, so that the reaction speed can be improved; the detector is arranged on the main body and extends into the reaction cavity so as to directly detect and analyze the concentration of the specific component in the liquid in the reaction cavity, and the efficiency is high.
Description
Technical Field
The application belongs to the technical field of biochemical analysis equipment, and particularly relates to a reactor and a reaction detection system.
Background
In biochemical analysis, such as blood analysis, it is often necessary to detect the content of each component in a sample. In the detection, the sample and the base solution are mixed for reaction, and then the mixed solution is detected. The sample testing is generally performed by using a disposable test tube to store the substrate solution, adding the sample into the test tube, stirring the liquid in the test tube, and then placing the test tube in the test tube for testing, however, this method is costly. Based on this, it is currently proposed to provide a reactor, to provide a reaction chamber and a drain pipe in the reactor, to add a substrate solution and a sample into the reaction chamber, to stir the reaction chamber, to place a detector in the reaction chamber for detection, to drain the reaction chamber after detection, and to add water to wash the reaction chamber for the next detection. However, in this way, each detection will require the use of a large-capacity sampling gun to add the substrate solution into the reaction chamber, and after the detection, the reaction chamber needs to be cleaned by using the spray head, which is inefficient.
SUMMERY OF THE UTILITY MODEL
An object of the embodiment of this application is to provide a reactor and reaction detecting system to detect at every turn and will need to use large capacity sampling gun to add base liquid in the reaction chamber in the reactor that solves existence among the prior art, detect the back, need use shower nozzle hydrojet to wash the reaction chamber, the problem of inefficiency.
In order to achieve the above purpose, the embodiment of the present application adopts the following technical solutions: providing a reactor comprising:
the device comprises a main body, a reaction cavity with an open top is arranged in the main body, an overflow pipe for discharging redundant liquid in the reaction cavity, an emptying pipe for emptying liquid in the reaction cavity and a liquid injection pipe for injecting liquid into the reaction cavity are arranged on the main body, the emptying pipe is communicated with the bottom of the reaction cavity, the overflow pipe is communicated with the top of the reaction cavity, the liquid injection pipe is communicated with the reaction cavity, a mounting hole is formed in the side surface of the main body, and the mounting hole is communicated with the reaction cavity;
the detector is arranged in the mounting hole and extends into the reaction cavity; and the number of the first and second groups,
and the stirrer extends into the reaction cavity.
In an alternative embodiment, the top of the main body is provided with an overflow cavity, the reaction cavity is positioned at the bottom of the overflow cavity, and the overflow pipe is positioned at the bottom of the overflow cavity.
In an optional embodiment, an overflow cover is installed at the top of the reaction chamber, an inverted cone-shaped chamber is arranged at the top of the overflow cover, a through hole communicating the bottom of the inverted cone-shaped chamber with the reaction chamber is arranged in the overflow cover, and the top of the overflow cover extends into the overflow chamber.
In an optional embodiment, the bottom of the overflow cover is provided with a conical cavity, and the through hole is communicated with the top of the conical cavity.
In an alternative embodiment, the reactor further comprises a support on which the body is mounted, the agitator being supported on the support.
In an alternative embodiment, the agitator comprises:
the magnetic suction piece is arranged at the bottom of the main body and is arranged at an interval with the reaction cavity;
the stirring piece is connected with the magnetic attraction piece in a magnetic adsorption manner and is arranged in the reaction cavity; and the number of the first and second groups,
and the motor is connected with the magnetic suction piece and is used for driving the magnetic suction piece to rotate.
In an optional embodiment, the agitator further comprises a tray mounted on the output shaft of the motor, the magnetic attraction member being mounted on the tray.
In an optional embodiment, at least two stirring sheets are arranged on the stirring piece, and the two stirring sheets are arranged at intervals.
It is another object of an embodiment of the present application to provide a reaction detection system, including:
a reactor as described in any preceding embodiment;
the substrate liquid bottle is used for storing the substrate liquid;
the waste liquid bottle is used for collecting waste liquid;
and the pump set is used for pumping the base liquid in the base liquid bottle to the reaction cavity through the liquid injection pipe, pumping the liquid overflowing from the reaction cavity to the waste liquid bottle through the overflow pipe, and pumping the liquid discharged from the emptying pipe to the waste liquid bottle.
In an optional embodiment, the pump set comprises a double peristaltic pump and a single peristaltic pump, the double peristaltic pump comprises a first pump pipe and a second pump pipe, two ends of the first pump pipe are respectively connected with the substrate liquid bottle and the liquid injection pipe, two ends of the second pump pipe are respectively connected with the waste liquid bottle and the overflow pipe, the single peristaltic pump comprises a third pump pipe, and two ends of the third pump pipe are respectively connected with the waste liquid bottle and the emptying pipe.
The beneficial effect of the reactor that this application embodiment provided lies in: compared with the prior art, the reactor of the embodiment of the application has the advantages that the overflow pipe, the liquid injection pipe and the emptying pipe which are communicated with the reaction cavity are arranged on the main body, so that the substrate liquid can be directly injected through the liquid injection pipe to clean the reaction cavity, and the substrate liquid is discharged through the emptying pipe after being cleaned, so that the cleaning efficiency is high; the overflow pipe is arranged, so that the substrate liquid can be conveniently and quickly quantified when being injected into the reaction cavity, and the stirrer is arranged to stir the liquid in the reaction cavity, so that the reaction speed can be improved; the detector is arranged on the main body and extends into the reaction cavity so as to directly detect and analyze the concentration of the specific component in the liquid in the reaction cavity, and the efficiency is high.
The reaction detection system provided by the embodiment of the application has the beneficial effects that: compared with the prior art, the reaction detection system of the embodiment of the application uses the reactor, has the technical effect of the reactor, and is not repeated herein.
Drawings
In order to more clearly illustrate the technical solutions in the embodiments of the present application, the drawings needed to be used in the embodiments or exemplary technical descriptions will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present application, and it is obvious for those skilled in the art to obtain other drawings without creative efforts.
FIG. 1 is a schematic structural diagram of a reaction detection system provided in an embodiment of the present application;
FIG. 2 is a schematic perspective view of a reactor provided in an embodiment of the present application;
FIG. 3 is a schematic top view of the reactor shown in FIG. 3;
FIG. 4 is a schematic view of a partial cross-sectional structure taken along line A-A of FIG. 3;
FIG. 5 is a schematic cross-sectional view taken along line B-B of FIG. 3;
fig. 6 is a schematic diagram of an exploded structure of a reactor provided in an embodiment of the present application.
Wherein, in the figures, the various reference numbers are given by way of example only:
100-a reaction detection system;
10-a reactor;
11-a body; 111-a reaction chamber; 112-an overflow chamber; 113-mounting holes; 114-a communication channel; 115-a housing chamber; 116-a convex ring;
121-overflow pipe; 122-liquid injection pipe; 123-an emptying pipe;
13-a detector; 131-electrode biosensors;
14-an overflow cover; 141-a reverse tapered cavity; 142-a via; 143-tapered cavities; 144-a step; 145-a sealing ring;
15-a stirrer; 151-stirring member; 1511-stirring sheet; 1512-a magnetic rod; 152-a magnetic element; 153-a motor; 154-a tray;
16-a support; 161-sealing ring;
20-a pump group; 21-double peristaltic pump; 211-a first pump tube; 212-a second pump tube; 22-single peristaltic pump; 221-a third pump tube;
31-base liquid bottle; 32-waste liquid bottle.
Detailed Description
In order to make the technical problems, technical solutions and advantageous effects to be solved by the present application clearer, the present application is further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the present application and are not intended to limit the present application.
It will be understood that when an element is referred to as being "secured to" or "disposed on" another element, it can be directly on the other element or be indirectly on the other element. When an element is referred to as being "connected to" another element, it can be directly connected to the other element or be indirectly connected to the other element.
Furthermore, the terms "first", "second" and "first" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of that feature. In the description of the present application, "a plurality" means two or more unless specifically limited otherwise. The meaning of "a number" is one or more unless specifically limited otherwise.
In the description of the present application, it is to be understood that the terms "center", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", and the like, indicate orientations or positional relationships based on those shown in the drawings, and are used only for convenience in describing the present application and for simplicity in description, and do not indicate or imply that the referenced devices or elements must have a particular orientation, be constructed and operated in a particular orientation, and thus, are not to be construed as limiting the present application.
In the description of the present application, it should be noted that, unless otherwise explicitly stated or limited, the terms "mounted," "connected," and "connected" are to be construed broadly, and may be, for example, a fixed connection, a detachable connection, or an integral connection; can be mechanically or electrically connected; either directly or indirectly through intervening media, either internally or in any other relationship. The specific meaning of the above terms in the present application can be understood by those of ordinary skill in the art as appropriate.
Reference throughout this specification to "one embodiment," "some embodiments," or "an embodiment" means that a particular feature, structure, or characteristic described in connection with the embodiment is included in one or more embodiments of the present application. Thus, appearances of the phrases "in one embodiment," "in some embodiments," "in other embodiments," or the like, in various places throughout this specification are not necessarily all referring to the same embodiment, but rather mean "one or more but not all embodiments" unless specifically stated otherwise. Furthermore, the particular features, structures, or characteristics may be combined in any suitable manner in one or more embodiments.
Referring to fig. 1 and 2, a reaction detection system 100 provided herein will now be described. The reaction detection system 100 comprises a reactor 10, a base liquid bottle 31, a waste liquid bottle 32 and a pump unit 20.
Referring to fig. 2, 4 and 5, the reactor 10 includes a main body 11, a detector 13 and a stirrer 15, wherein: the main body 11 has a reaction chamber 111 formed therein, and the top of the reaction chamber 111 is open so that a liquid such as a sample can be filled into the reaction chamber 111.
The main body 11 is provided with an overflow pipe 121, a liquid injection pipe 122 and an emptying pipe 123, and the overflow pipe 121, the liquid injection pipe 122 and the emptying pipe 123 are all communicated with the reaction cavity 111. The liquid injection tube 122 is used for injecting liquid into the reaction chamber 111, such as a base liquid into the reaction chamber 111, to clean the reaction chamber 111 with the base liquid, and in addition, the base liquid may be injected into the reaction chamber 111 after cleaning, so as to react with the sample, and facilitate detection of the sample. The overflow pipe 121 is used to discharge the excess liquid in the reaction chamber 111, so that the liquid in the reaction chamber 111 can be quantified through the overflow pipe 121, for example, when the excess substrate liquid is filled into the reaction chamber 111, the excess substrate liquid will be discharged through the overflow pipe 121 to ensure the quantification of the substrate liquid in the reaction chamber 111. The evacuation pipe 123 is used to evacuate the liquid from the reaction chamber 111, such as after cleaning the reaction chamber 111, discharging the liquid from the reaction chamber 111 through the evacuation pipe 123, and after detecting, discharging the liquid from the reaction chamber 111 through the evacuation pipe 123. Through setting up notes liquid pipe 122 to annotate the base liquid, need not to use the sampling gun, it is efficient. The overflow pipe 121 is used to quantify the liquid in the reaction chamber 111, so that the efficiency can be further improved. The emptying pipe 123 is arranged for emptying, so that the cleaning and the use are convenient, and the efficiency is improved.
The side of main part 11 is equipped with mounting hole 113, and mounting hole 113 communicates with reaction chamber 111, and detector 13 is installed in mounting hole 113 to make detector 13 stretch into reaction chamber 111, can directly detect the specific component in the liquid in reaction chamber 111 like this, realize biochemical analysis, efficient.
And the pump unit 20 is used for pumping the base liquid in the base liquid bottle 31 to the reaction cavity 111 through the liquid injection pipe 122, pumping the liquid overflowing from the reaction cavity 111 to the waste liquid bottle 32 through the overflow pipe 121, and pumping the liquid discharged from the emptying pipe 123 to the waste liquid bottle 32. Therefore, the substrate liquid can be automatically filled into the reaction cavity 111, and the substrate liquid can be automatically discharged and overflow discharged, so that the efficiency is improved.
Compared with the prior art, in the reactor 10 provided by the embodiment of the present application, the overflow pipe 121, the liquid injection pipe 122 and the emptying pipe 123 which are communicated with the reaction chamber 111 are arranged on the main body 11, so that the substrate liquid can be directly injected through the liquid injection pipe 122 to clean the reaction chamber 111, and after cleaning, the substrate liquid is discharged through the emptying pipe 123, and the cleaning efficiency is high; the overflow pipe 121 is provided to facilitate and rapidly quantify the substrate liquid when the substrate liquid is injected into the reaction chamber 111, and the stirrer 15 is provided to stir the liquid in the reaction chamber 111 to increase the reaction speed; the detector 13 is installed on the main body 11 such that the detector 13 is extended into the reaction chamber 111 to directly detect the concentration of a specific component in the liquid in the analysis reaction chamber 111 with high efficiency.
Compared with the prior art, the reaction detection system 100 provided by the embodiment of the application uses the reactor 10, can realize automatic and rapid liquid injection, cleaning, detection and emptying of the reactor 10, and is high in efficiency.
In one embodiment, the pump stack 20 includes a dual peristaltic pump 21 and a single peristaltic pump 22. The double peristaltic pump 21 is a peristaltic pump having two pump tubes. The single peristaltic pump 22 refers to a peristaltic pump having one pump tube. The double peristaltic pump 21 has a first pump tube 211 and a second pump tube 212, two ends of the first pump tube 211 are respectively connected to the substrate liquid bottle 31 and the liquid injection tube 122, two ends of the second pump tube 212 are respectively connected to the waste liquid bottle 32 and the overflow tube 121, so that when the double peristaltic pump 21 works, the first pump tube 211 pumps the substrate liquid to be injected into the reaction chamber 111, and the second pump tube 212 makes the overflow tube 121 generate negative pressure to timely pump out the liquid overflowing from the reaction chamber 111. The single peristaltic pump 22 has a third pump tube 221, and both ends of the third pump tube 221 are respectively connected to the waste liquid bottle 32 and the emptying tube 123, so that the reaction chamber 111 can be better emptied when the single peristaltic pump 22 is operated. The double peristaltic pumps 21 and the single peristaltic pump 22 are used, the structure is simple, the efficiency is high, in addition, the control can be convenient, the integration level can be improved by using the double peristaltic pumps 21, and the size is reduced. It will be appreciated that other pumping elements may be used, such as a vane pump, centrifugal pump, plunger pump, etc. In addition, three pumping parts can be arranged, two ends of one pumping part are connected with the base liquid bottle 31 and the liquid injection pipe 122, two ends of one pumping part are connected with the waste liquid bottle 32 and the overflow pipe 121, and two ends of the third pumping part are respectively connected with the waste liquid bottle 32 and the emptying pipe 123, so that the base liquid can be automatically filled into the reaction cavity 111, the overflow liquid can be discharged, and the reaction cavity 111 can be emptied.
In one embodiment, referring to fig. 4 and 6, the detector 13 may use an electrode biosensor 131. The electrode type biosensor 131 is based on a mildew membrane method, and generates an electric signal after a sample to be measured (hereinafter, referred to as a sample) reacts with a mildew membrane, and analyzes the concentration of a measured item by recognizing the electric signal by the electrode of the electrode type biosensor 131. The electrode life and accuracy based on the mildew film method can be attenuated to different degrees due to the increase of the number of samples, the concentration of the samples, the working time and other factors. Wherein the sample concentration directly affects the accuracy and the service life of the electrode. However, the electrode type biosensor 131 cannot directly measure the sample solution. At this time, the substrate solution may be a buffer solution, and the reaction chamber 111 is filled with the buffer solution until the excess overflows. And then a sample to be measured (namely, a sample to be measured) is added into the reaction cavity 111 and is uniformly stirred by the stirrer 15, and the sample is diluted by a certain multiple, so that the accuracy and the service life of the electrode type biosensor 131 are guaranteed to a certain extent. It will be appreciated that other biosensors may be used for the detector 13, such as light sensitive sensors, etc.
In the above embodiment, the diameter of the electrode leakage reaction chamber 111 portion of the electrode type biosensor 131 is in the range of 5mm to 8mm, for example, the diameter of the electrode leakage reaction chamber 111 portion is 5mm, 6mm, 7mm, 8mm, etc. so as to be able to protrude into the reaction chamber 111. The area of the electrode leakage reaction cavity 111 part is 25-30 square millimeters, for example, the area of the electrode leakage reaction cavity 111 part is 25mm 2 、26mm 2 、27mm 2 、28mm 2 、28.26mm 2 、29mm 2 、30mm 2 And the like, so as to ensure a larger contact area between the electrode and the liquid in the reaction cavity 111 and ensure the detection accuracy.
In one embodiment, referring to fig. 3, 4 and 5, an overflow chamber 112 is disposed on the top of the main body 11, the reaction chamber 111 is disposed on the bottom of the overflow chamber 112, and the overflow pipe 121 is disposed on the bottom of the overflow chamber 112. The overflow cavity 112 is arranged so that the liquid overflowing from the reaction cavity 111 can directly enter the overflow cavity 112, thereby avoiding affecting the reaction cavity 111 and better quantifying the liquid in the reaction cavity 111.
In one embodiment, referring to fig. 3 to 6, an overflow cover 14 is installed on the top of the reaction chamber 111, the top of the overflow cover 14 extends into the overflow chamber 112, an inverted cone-shaped chamber 141 is installed on the top of the overflow cover 14, a through hole 142 is installed in the overflow cover 14, and the through hole 142 communicates the bottom of the inverted cone-shaped chamber 141 with the reaction chamber 111, so that the liquid in the reaction chamber 111 can enter the inverted cone-shaped chamber 141 through the through hole and then overflow from the top of the overflow cover 14 to the overflow chamber 112, so as to better quantify the liquid in the reaction chamber 111. And set up reverse cone shape chamber 141 at the top of overflow lid 14, can annotate the sample to reverse cone shape chamber 141, can stretch into through-hole 142 with the syringe needle of application of sample rifle from reverse cone shape chamber 141 to the application of sample in reaction chamber 111, the application of sample is more convenient, and reverse cone shape chamber 141 can play the guide effect.
In one embodiment, the bottom of the overflow cover 14 is opened with a tapered cavity 143, and the through hole 142 is communicated with the top of the tapered cavity 143, so that when the reaction chamber 111 is filled with liquid, the liquid can conveniently enter the through hole 142 from the tapered cavity 143 to overflow from the top of the overflow cover 14 through the inverted tapered cavity 141, and the resistance is reduced.
In one embodiment, the body 11 is provided with a convex ring 116, the convex ring 116 is arranged around the reaction chamber 111, the convex ring 116 is arranged at the bottom of the overflow chamber 112, the upper end of the overflow cover 14 is provided with a step 144 protruding radially outwards, and the step 144 is supported on the convex ring 116 so as to install the overflow cover 14.
In one embodiment, a sealing ring 145 is disposed between the protruding ring 116 and the step 144 to ensure that the liquid overflowing from the overflow cover 14 does not penetrate into the reaction chamber 111, so as to ensure the accuracy of the sample detection in the reaction chamber 111.
In one embodiment, referring to fig. 1, 2 and 4, the main body 11 is provided with two mounting holes 113, and each mounting hole 113 is mounted on the detector 13. The two detectors 13 may be identical to detect the same component simultaneously to ensure accuracy of detection. Of course, the two detectors 13 may also be different to detect different components. It will be appreciated that only one mounting hole 113 may be provided in the body 11, and correspondingly one detector 13. Of course, three, four, etc. mounting holes 113 may be provided in the main body 11, and accordingly, three, four, etc. detectors 13 may be provided.
In one embodiment, referring to fig. 5 and 6, the main body 11 is provided with a communication channel 114, and the communication channel 114 communicates the liquid injection pipe 122 with the bottom of the reaction chamber 111, so that when the substrate liquid is injected, the substrate liquid can be squeezed out of the air at the bottom of the reaction chamber 111, and the amount of the injected liquid in the reaction chamber 111 can be kept consistent. It is understood that the liquid inlet 122 may communicate with the middle region of the reaction chamber 111 in the height direction.
In one embodiment, referring to fig. 4 to fig. 6, the stirrer 15 includes a magnetic member 152, a stirring member 151, and a motor 153, wherein the magnetic member 152 is disposed at the bottom of the main body 11, the magnetic member 152 is spaced apart from the reaction chamber 111, and the motor 153 is connected to the magnetic member 152 to drive the magnetic member 152 to rotate through the motor 153. The stirring member 151 is disposed in the reaction chamber 111, and the stirring member 151 is magnetically attracted to the magnetic attraction member 152, that is, the magnetic attraction member 152 magnetically attracts the stirring member 151, so that when the magnetic attraction member 152 rotates, the stirring member 151 can be driven to rotate in the reaction chamber 111. The structure only places the stirring piece 151 in the reaction chamber 111, so that the volume occupied in the reaction chamber 111 is reduced, and the installation and the control are convenient. It is understood that the support rod may be used to support the stirring member 151 while the stirring member 151 is extended into the reaction chamber 111.
In one embodiment, the magnetic attraction member 152 may use a magnet, an electromagnet, or other magnetically attracted structural members.
In one embodiment, the magnetic rod 1512 is disposed in the stirring element 151, and the magnetic rod 1512 can be made of a magnetically attractable material such as an iron member, a magnet member, or a nickel member, so that the stirring element 151 can be magnetically attracted by the magnetic element 152. This configuration allows the magnetic rod 1512 to be made smaller, and reduces the influence of the magnetic rod 1512 on the liquid in the reaction chamber 111. It is understood that the stirring member 151 may be made of a magnetically adsorptive material such as a ferrous member, a magnetic member, or a nickel member.
In one embodiment, at least two stirring pieces 1511 are disposed on the stirring member 151, and the two stirring pieces 1511 are spaced apart from each other, so that the liquid in the reaction chamber 111 can be better stirred when the stirring member 151 rotates. It is understood that only one stirring piece 1511 may be provided on the stirring member 151.
In one embodiment, two stirring pieces 1511 are provided on the stirring member 151 to make the stirring member 151 small and ensure a good stirring effect. It is understood that three, four, etc. stirring pieces 1511 may be provided on the stirring member 151.
In one embodiment, the agitator 15 further includes a tray 154, the tray 154 is mounted on an output shaft of the motor 153, the magnetic attraction 152 is mounted on the tray 154, and the tray 154 is configured to mount and support the magnetic attraction 152 and facilitate coupling the magnetic attraction 152 to the motor 153. It will be appreciated that the magnetically attractive element 152 may also be directly connected to the output shaft of the motor 153.
In one embodiment, the magnetic members 152 are two, and the magnetic poles of the two magnetic members 152 are oppositely arranged to position the stirring member 151. It is understood that the magnetic attracting elements 152 can be provided in one, three, etc. number.
In one embodiment, the magnetic attraction 152 may be secured to the tray 154 using glue. It will be appreciated that the magnetically attractive element 152 may also be conveniently secured to the tray 154 by adhesive, interference fit, or the like.
In one embodiment, the bottom of the main body 11 is provided with a receiving cavity 115, and the magnetic attraction member 152 is disposed in the receiving cavity 115, so that the magnetic attraction member 152 is closer to the stirring member 151, and the magnetic attraction member 152 attracts the stirring member 151.
In one embodiment, referring to fig. 2, 4 and 6, the reactor 10 further includes a support 16, the main body 11 is mounted on the support 16, and the agitator 15 is supported on the support 16. The support 16 is provided to facilitate mounting of the fixing body 11 and the agitator 15, and to facilitate mounting and use.
In one embodiment, the motor 153 may be mounted on the support base 16 to support the motor 153 through the support base 16 to support the magnetic attracting member 152.
In one embodiment, a sealing ring 161 is disposed between the main body 11 and the support 16, and the sealing ring 161 is disposed around the accommodating cavity 115 to prevent impurities from entering the accommodating cavity 115, so as to protect the magnetic attraction member 152 well.
In one embodiment, referring to fig. 4 to fig. 6, the diameter of the reaction chamber 111 is in a range of 5-8mm, for example, the diameter of the reaction chamber 111 can be 5mm, 6mm, 7mm, 8mm, etc., so as to make the reaction chamber 111 smaller, reduce the usage of sample and substrate solution, and increase the reaction and detection speed and efficiency. If the diameter of the reaction chamber 111 is too small, the diameter of the stirring bar 151 becomes small, and the depth of the reaction chamber 111 becomes too large, so that the stirring effect is deteriorated and the efficiency is deteriorated. When the diameter of the reaction chamber 111 is too large, the depth of the reaction chamber 111 is small, and a vortex is generated during stirring, which is inconvenient for detection.
In one embodiment, the depth of the reaction chamber 111 ranges from 22mm to 28mm, e.g., the depth of the reaction chamber 111 can be 22mm, 23mm, 24mm, 25mm, 26mm, 27mm, 28mm, etc. So as to avoid the depth of the reaction chamber 111 from being too small or too large, and facilitate the stirring by the stirrer 15. If the depth of the reaction chamber 111 is too large, the stirring effect is deteriorated and the efficiency is deteriorated. When the depth of the reaction chamber 111 is small, a vortex is generated during stirring, which is inconvenient for detection.
In one embodiment, the volume of the reaction chamber 111 is in the range of 350-450 μ l, for example, the volume of the reaction chamber 111 may be 350mm 3 、360mm 3 、370mm 3 、380mm 3 、390mm 3 、400mm 3 、410mm 3 、420mm 3 、430mm 3 、440mm 3 、450mm 3 Etc. to reduce the use of substrate fluids and samples.
In one embodiment, the outer diameter of the stirrer 15 is 0.4mm to 0.8mm smaller than the inner diameter of the reaction chamber 111, such as the outer diameter of the stirrer 15 is 0.4mm, 0.5mm, 0.6mm, 0.7mm, 0.8mm smaller than the inner diameter of the reaction chamber 111 so that the stirrer 15 can rotate flexibly in the reaction chamber 111 and stir the liquid in the reaction chamber 111 well.
In one embodiment, the distance between the tray 154 and the bottom of the reaction chamber 111 is in a range of 0.8mm to 1.5mm, for example, the distance between the tray 154 and the bottom of the reaction chamber 111 is 0.8mm, 0.9mm, 1mm, 1.1mm, 1.2mm, 1.3mm, 1.4mm, 1.5mm, etc., so as to ensure that the magnetic attraction member 152 can attract the stirring member 151 well. When the distance between the tray 154 and the bottom of the reaction chamber 111 is too small, the structural strength of the bottom of the reaction chamber 111 is poor; when the distance between the tray 154 and the bottom of the reaction chamber 111 is too large, the magnetic attraction of the magnetic attraction member 152 to the stirring member 151 is poor, which is not favorable for driving the stirring member 151 to rotate.
In one embodiment, each magnetic member 152 has a semi-circular shape, and the two magnetic members 152 form a circular shape. It is understood that the magnetic element 152 can be provided in other shapes.
In one embodiment, the diameter of the magnetic element 152 is in the range of 10-20mm, for example, the diameter of the magnetic element 152 can be 10mm, 11mm, 12mm, 13mm, 14mm, 15mm, 16mm, 17mm, 18mm, 19mm, 20mm, etc. to ensure that the magnetic element 152 can well attract the stirring element 151. When the diameter of the magnetic attraction piece 152 is too small, the magnetic attraction force is small, and the magnetic attraction force of the magnetic attraction piece 152 on the stirring piece 151 is poor, so that the stirring piece 151 is not driven to rotate; when the diameter of the magnetic member 152 is too large, it takes up too much space, and miniaturization of the reactor 10 is not utilized.
In one embodiment, the diameter of the through hole 142 on the overflow cover 14 ranges from 0.8mm to 1.2mm, for example, the diameter of the through hole 142 can be 0.8mm, 0.9mm, 1mm, 1.1mm, 1.2mm, etc., so as to facilitate the liquid passing through the through hole 142 and facilitate the processing. When the diameter of the through hole 142 on the overflow cover 14 is too small, the liquid passing resistance is larger, and overflow and sample adding are not utilized; when the diameter of the through hole 142 on the overflow cover 14 is too large, the reaction chamber 111 is easily interfered by the external environment, which affects the detection effect.
The working process of the reaction detection system 100 according to the embodiment of the present application is as follows:
pouring: the reaction chamber 111 is filled with a buffer.
The double peristaltic pump 21 operates to pump the buffer solution into the reaction chamber 111, the motor 153 drives the magnetic attraction member 152 to rotate, and drives the stirring member 151 to rotate and stir until the buffer solution is excessive and overflows, and the overflowing buffer solution is pumped out, so that the liquid amount in the reaction chamber 111 is in a dynamic balance state.
Cleaning: the reaction chamber 111, the overflow chamber 112 and the respective pipes of the reaction detection system are cleaned, and the portion of the detector 13 extending into the reaction chamber 111 is cleaned.
The single peristaltic pump 22 is operated to drain the reaction chamber 111, and then the double peristaltic pump 21 is operated to inject the buffer solution into the reaction chamber 111 and keep the stirring member 151 rotating until the electric signal of the electrode type biosensor 131 drops to a set point, which is referred to as a zero voltage V0, V0 may be 580 mv, and V0 may be set to other positions, and then the reaction detecting system 100 enters a ready state.
Calibration: the electrode type biosensor 131 is calibrated.
In order to ensure the accuracy of the system test, the electrode biosensor 131 is calibrated with a standard solution with a standard concentration of C1 at each time interval. Calibrating the correlation between the current electrode test signal and the actual measured object concentration;
after the reaction detecting system 100 enters the ready state, the stirring element 151 is driven to stir, and then a specified amount of standard solution, such as 25 microliters, 26 microliters, 27 microliters, etc., is precisely added into the reaction chamber 111 by using a manual quantitative sample injector or an automatic quantitative sample injector, which can be specifically set as required. After entering the reaction chamber 111, the standard solution is rapidly mixed with the buffer solution under the stirring effect. The reaction detecting system 100 records the time of sample application, counts the designated time after sample application, and then reads the voltage V1 of the electrical signal collected by the electrode type biosensor 131. And calculating the slope K according to the voltage V1 corresponding to the standard solution and the concentration C1 of the standard solution. After calibration is completed, the reaction detection system 100 performs a cleaning step and enters a ready state.
And (3) sample measurement: and detecting the sample.
After the reaction detecting system 100 completes the calibration step and enters the ready state, the stirring element 151 is driven to stir, and then a manual quantitative sample injector or an automatic quantitative sample injector is used to add a specified amount of sample into the reaction chamber 111, and the sample enters the reaction chamber 111 and then rapidly reaches a uniform mixing state with the buffer solution under the stirring effect. The reaction detection system 100 can record the time of sample addition, count the designated time after sample addition, and then read the electrical signal V2 collected by the electrode type biosensor 131. The sample concentration C2 was calculated according to the relation K = V1/C1= V2/C2 to achieve sample detection.
After the test is completed, the reaction detection system 100 performs a cleaning step, and enters a ready state for the next detection.
The sample volume of the system is 25 microliter, the volume of the reaction cavity 111 is 400 microliter, and the sample is diluted by 16 times when being tested.
The detector 13 of the reaction detecting system 100 can use different electrode biosensors 131, such as by replacing the electrode mold film of the electrode biosensors 131, so as to test the concentration of glucose, lactic acid, glutamic acid, glutamine, lysine, ethanol, methanol, glycerol, xylose, and other components.
Taking glucose as an example, the optimal test concentration range is 0.003-0.563 g/L, and the general sample solubility range is 0-9 g/L, based on this consideration, the dilution factor range of the reaction detection system 100 of this embodiment is 10-20 times, such as 10 times, 11 times, 12 times, 13 times, 14 times, 15 times, 16 times, 17 times, 18 times, 19 times, 20 times, etc. Too small a dilution factor may affect the accuracy and repeatability of testing high-value samples, and may also cause the test voltage to exceed the upper limit of the voltage of the electrode biosensor 131 and fail to measure the true concentration. The dilution factor is too large, which may affect the accuracy and repeatability of low-value samples, or the test voltage may be too low, for example, the voltage is lower than the zero voltage, so that the real concentration cannot be measured.
The above description is only exemplary of the present application and should not be taken as limiting the present application, as any modification, equivalent replacement, or improvement made within the spirit and principle of the present application should be included in the protection scope of the present application.
Claims (10)
1. A reactor, comprising:
the device comprises a main body, a reaction cavity with an open top is arranged in the main body, an overflow pipe for discharging redundant liquid in the reaction cavity, an emptying pipe for emptying liquid in the reaction cavity and a liquid injection pipe for injecting liquid into the reaction cavity are arranged on the main body, the emptying pipe is communicated with the bottom of the reaction cavity, the overflow pipe is communicated with the top of the reaction cavity, the liquid injection pipe is communicated with the reaction cavity, a mounting hole is formed in the side surface of the main body, and the mounting hole is communicated with the reaction cavity;
the detector is arranged in the mounting hole and extends into the reaction cavity; and the number of the first and second groups,
and the stirrer extends into the reaction cavity.
2. The reactor of claim 1, wherein: the top of the main body is provided with an overflow cavity, the reaction cavity is positioned at the bottom of the overflow cavity, and the overflow pipe is positioned at the bottom of the overflow cavity.
3. The reactor of claim 2, wherein: the overflow cover is installed at the top of the reaction cavity, the inverted cone-shaped cavity is arranged at the top of the overflow cover, the inverted cone-shaped cavity is communicated with the bottom of the inverted cone-shaped cavity and the through hole of the reaction cavity, and the top of the overflow cover extends into the overflow cavity.
4. The reactor of claim 3, wherein: the bottom of the overflow cover is provided with a conical cavity, and the through hole is communicated with the top of the conical cavity.
5. The reactor of any one of claims 1 to 4, wherein: the reactor also comprises a support, the main body is arranged on the support, and the stirrer is supported on the support.
6. The reactor according to any one of claims 1 to 4, wherein the agitator comprises:
the magnetic suction piece is arranged at the bottom of the main body and is arranged at an interval with the reaction cavity;
the stirring piece is connected with the magnetic attraction piece in a magnetic adsorption manner and is arranged in the reaction cavity; and the number of the first and second groups,
and the motor is connected with the magnetic suction piece and used for driving the magnetic suction piece to rotate.
7. The reactor of claim 6, wherein: the stirrer further comprises a tray, the tray is installed on the output shaft of the motor, and the magnetic attraction piece is installed on the tray.
8. The reactor of claim 6, wherein: the stirring piece is provided with at least two stirring sheets, and the two stirring sheets are arranged at intervals.
9. A reaction detection system, comprising:
a reactor according to any one of claims 1 to 8;
the substrate liquid bottle is used for storing the substrate liquid;
a waste liquid bottle for collecting waste liquid;
and the pump set is used for pumping the base liquid in the base liquid bottle to the reaction cavity through the liquid injection pipe, pumping the liquid overflowing from the reaction cavity to the waste liquid bottle through the overflow pipe, and pumping the liquid discharged from the emptying pipe to the waste liquid bottle.
10. The reaction detection system of claim 9, wherein: the pump package is including two peristaltic pumps and single peristaltic pump, two peristaltic pumps have first pump line and second pump line, the both ends of first pump line are connected respectively the basement liquid bottle with annotate the liquid pipe, the both ends of second pump line are connected respectively the waste liquid bottle with the overflow pipe, single peristaltic pump has the third pump line, the both ends of third pump line are connected respectively the waste liquid bottle with the evacuation pipe.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202221633034.0U CN217212408U (en) | 2022-06-28 | 2022-06-28 | Reactor and reaction detection system |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202221633034.0U CN217212408U (en) | 2022-06-28 | 2022-06-28 | Reactor and reaction detection system |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN217212408U true CN217212408U (en) | 2022-08-16 |
Family
ID=82779413
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202221633034.0U Active CN217212408U (en) | 2022-06-28 | 2022-06-28 | Reactor and reaction detection system |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN217212408U (en) |
-
2022
- 2022-06-28 CN CN202221633034.0U patent/CN217212408U/en active Active
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105784571B (en) | Double-pool measuring method and device for specific reaction protein CRP | |
| CN107091875B (en) | A kind of hand-held aspiration-type electrochemical detection device automatically processing sample | |
| CN111721956A (en) | Liquid path system of full-automatic biochemical analyzer | |
| CN217212408U (en) | Reactor and reaction detection system | |
| CN113116348B (en) | Continuous arterial blood detection device | |
| CN114236161A (en) | Liquid suction structure and liquid supply device for immunoassay | |
| CN217385319U (en) | Sample analyzer and electrolyte measuring mechanism | |
| CN217277890U (en) | Sample analyzer | |
| JP7123993B2 (en) | Sensor unit and cell culture analyzer equipped with same | |
| CN215641281U (en) | Liquid path system of biological sample detection analyzer and biological sample detection analyzer | |
| CN209946178U (en) | Liquid path system of full-automatic biochemical analyzer | |
| JP2021153576A (en) | Cell culture analysis device and cell culture analysis method using the same | |
| CN103105425B (en) | Water quality detection device and method | |
| CN209745975U (en) | Efficient full-automatic urine detector | |
| CN217543141U (en) | Integrated interface device and biochemical analyzer | |
| CN202049162U (en) | Conventional inspection instrument for whole blood transaminase | |
| CN207281087U (en) | A kind of micro- water gaging sampling device | |
| CN219475624U (en) | Waste liquid tank mechanism and full-automatic inspection instrument | |
| CN217212404U (en) | Biochemical analyzer | |
| CN213398198U (en) | Liquid storage tank, liquid path system and sample analyzer | |
| CN214310561U (en) | Full-automatic specific protein analyzer with rotatable display screen | |
| CN216792137U (en) | Automatic add trace moisture tester of flowing back | |
| CN114323783B (en) | Sampling method, sampling assembly and sample analyzer | |
| CN215312445U (en) | In vitro diagnosis testing device | |
| JP2021153404A (en) | Additive agent supply member and cell culture analyzer comprising the same |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant |