CN217077610U

CN217077610U - Full-automatic animal epidemic disease nucleic acid extraction and fluorescence PCR detect all-in-one

Info

Publication number: CN217077610U
Application number: CN202221046421.4U
Authority: CN
Inventors: 刘颖; 周刚; 谷楠; 苏伟果
Original assignee: Jiangsu Murun Biotechnology Co ltd
Current assignee: Jiangsu Murun Biotechnology Co ltd
Priority date: 2022-04-29
Filing date: 2022-04-29
Publication date: 2022-07-29
Anticipated expiration: 2032-04-29

Abstract

The utility model discloses a full-automatic animal epidemic disease nucleic acid draws and fluorescence PCR detects all-in-one, including the detection case, the same baffle of relative one side inner wall fixedly connected with of detection case one, the inside of detection case is provided with the conveyer belt, the conveyer belt is the symmetric distribution, the top outer wall fixedly connected with fixed disk of conveyer belt, the top outer wall fixedly connected with motor one of fixed disk, the output shaft one end fixedly connected with rolling disc of motor one, one the top outer wall fixedly connected with a plurality of stop collar of rolling disc, the inner wall of stop collar is pegged graft and is had the sample box. Through setting up the conveyer belt, the below of magnetic rod is carried to the sample box that a conveyer belt will be equipped with the magnetic bead, will have the nucleic acid magnetic bead by the magnetic rod and adsorb to put into the nucleic acid extraction box that drives the liquid medicine, another conveyer belt is sent nucleic acid extraction box to detection mechanism's below and is detected, and then realizes drawing, detects the integration, has improved work efficiency greatly.

Description

Full-automatic animal epidemic disease nucleic acid extraction and fluorescence PCR detection all-in-one machine

Technical Field

The utility model relates to a nucleic acid extraction and PCR detect technical field, more specifically say, it relates to a full-automatic animal epidemic disease nucleic acid extraction and fluorescence PCR detect all-in-one.

Background

The methods for extracting nucleic acid are classified into a centrifugal column method and a magnetic bead method, and the PCR is called a polymerase chain reaction in its entirety, which is a molecular biology technique for amplifying and amplifying a specific DNA fragment, and can be regarded as a special DNA replication in vitro, and the greatest feature of PCR is that a trace amount of DNA can be greatly increased, so that it is very useful, and it can be amplified and compared by PCR as long as a few DNA fragments can be separated from the remains of ancient organisms and historical figures in fossil, and the hair, skin or blood left several decades ago.

The existing machine is used for extracting nucleic acid and performing fluorescence PCR detection separately, and cannot perform detection at the first time of extracting nucleic acid, so that the working efficiency is low, and therefore improvement is needed.

SUMMERY OF THE UTILITY MODEL

Aiming at the defects of the prior art, the utility model aims to provide a full-automatic animal epidemic disease nucleic acid extraction and fluorescence PCR detection integrated machine, which comprises a detection box, wherein the inner wall of the opposite side of the detection box is fixedly connected with a first baffle plate, a conveyor belt is arranged in the detection box and is symmetrically distributed, the outer wall of the top part of the conveyor belt is fixedly connected with a fixed disc, the outer wall of the top part of the fixed disc is fixedly connected with a first motor, one end of the output shaft of the first motor is fixedly connected with a rotating disc, the outer wall of the top part of one rotating disc is fixedly connected with a plurality of limit sleeves, the inner wall of each limit sleeve is inserted with a sample box, the outer wall of the top part of the other rotating disc is connected with a plurality of nucleic acid extraction boxes through buckles, the outer wall of the top part of the detection box is fixedly connected with a second motor, one end of the output shaft of the second motor is fixedly connected with a connecting block, the bottom outer wall fixedly connected with electric putter one of connecting block, electric putter one's piston rod one end fixedly connected with magnetic frame, a plurality of magnetic rod of the bottom outer wall fixedly connected with of magnetic frame, nucleic acid draws box and sample box all with magnetic rod looks adaptation, the inside of detection case is provided with detection mechanism, magnetic bead and adds mechanism, liquid medicine and adds mechanism and shading subassembly.

In order to achieve the above purpose, the utility model provides a following technical scheme:

preferably, the magnetic bead adding mechanism comprises a magnetic bead box, the magnetic bead box is fixedly connected with the inner wall of the detection box, the inner wall of the magnetic bead box is provided with a first mounting opening, the inner walls on the two sides of the mounting opening are rotatably connected with the same material blocking convex disc through bearings, the outer wall of the magnetic bead box is fixedly connected with a third motor, and one end of an output shaft of the third motor is fixedly connected with the material blocking convex disc.

Preferably, the liquid medicine adding mechanism comprises a second electric push rod, a second mounting opening is formed in the inner wall of one side of the detection box, the inner wall of the second mounting opening is fixedly connected with the second electric push rod, and a medicine adding gun is fixedly connected to one end of a piston rod of the second electric push rod.

Preferably, the detection mechanism comprises a laser emitter, the laser emitter is fixedly connected with the inner wall of the detection box, and a fluorescence detection device is arranged on the inner wall of the top of the detection box.

Preferably, the light shielding assembly comprises a second partition plate, the second partition plate is fixedly connected with the first partition plate, a sliding groove is formed in the outer wall of one side of the second partition plate, a light shielding plate is slidably connected to the inner wall of the sliding groove, a pull rope is fixedly connected to the outer wall of the top of the light shielding plate, a take-up reel is fixedly connected to the outer wall of an output shaft of the second motor, and the outer wall of the take-up reel is fixedly connected with the pull rope.

Compared with the prior art, the utility model discloses possess following beneficial effect:

1. the utility model discloses in, through setting up the conveyer belt, a conveyer belt will be equipped with the sample box of magnetic bead, carries the below of magnetic rod, will have the nucleic acid magnetic bead by the magnetic rod and adsorb to put into the nucleic acid extraction box that drives the liquid medicine, another conveyer belt is sent nucleic acid extraction box to detection mechanism's below and is detected, and then realizes drawing, detect the integration, has improved work efficiency greatly.

2. The utility model discloses in, through setting up the light screen, when two drive magnetic rod of motor move nucleic acid and draw the box directly over, three motors pull up the light screen through the take-up reel, draw the below that the detection device was carried to the box with nucleic acid through the conveyer belt, and three motors drive the magnetic rod again and reset, fall the light screen simultaneously, guarantee at the in-process that detects, reduce the interference that outside light brought, guarantee the accuracy of testing result.

Drawings

Fig. 1 is a schematic view of the overall structure of the present invention;

FIG. 2 is a schematic view of the nucleic acid extraction cassette of the present invention;

fig. 3 is the material stopping convex disc of the utility model.

1. A detection box; 2. a magnetic bead box; 3. a conveyor belt; 4. fixing the disc; 5. a first motor; 6. rotating the disc; 7. A limiting sleeve; 8. a sample cartridge; 9. a magnetic bar; 10. a magnetic frame; 11. an electric push rod I; 12. connecting blocks; 13. a second motor; 14. a take-up reel; 15. pulling a rope; 16. a first clapboard; 17. a second electric push rod; 18. a dosing gun; 19. a nucleic acid extraction cassette; 20. a visor; 21. a second clapboard; 22. a laser transmitter; 23. a sliding groove; 24. a fluorescence detection device; 25. a material blocking convex disc; 26. and a third motor.

Detailed Description

Referring to fig. 1 to fig. 3, the embodiment further illustrates a full-automatic animal epidemic disease nucleic acid extraction and fluorescence PCR detection integrated machine provided by the present invention.

A full-automatic animal epidemic disease nucleic acid extraction and fluorescence PCR detection integrated machine comprises a detection box 1, the inner wall of one opposite side of the detection box 1 is fixedly connected with a first partition plate 16, a conveyor belt 3 is arranged in the detection box 1, the conveyor belt 3 is symmetrically distributed, the outer wall of the top of the conveyor belt 3 is fixedly connected with a fixed disc 4, the outer wall of the top of the fixed disc 4 is fixedly connected with a motor 5, one end of an output shaft of the motor 5 is fixedly connected with a rotating disc 6, the outer wall of the top of one rotating disc 6 is fixedly connected with a plurality of limiting sleeves 7, the inner wall of each limiting sleeve 7 is inserted with a sample box 8, the outer wall of the top of the other rotating disc 6 is connected with a plurality of nucleic acid extraction boxes 19 through buckles, the outer wall of the top of the detection box 1 is fixed with a motor II 13, one end of the output shaft of the motor II 13 is fixedly connected with a connecting block 12, and the outer wall of the bottom of the connecting block 12 is fixedly connected with an electric push rod I11, piston rod one end fixedly connected with magnetic frame 10 of electric putter 11, a plurality of magnetic rod 9 of the bottom outer wall fixedly connected with of magnetic frame 10, nucleic acid draws box 19 and sample box 8 all with magnetic rod 9 looks adaptation, the inside of detection case 1 is provided with detection mechanism, the magnetic bead adds the mechanism, liquid medicine adds mechanism and shading subassembly, the sample box 8 of magnetic bead will be equipped with to a conveyer belt 3, carry the below of magnetic rod 9, it adsorbs to have the nucleic acid magnetic bead by magnetic rod 9, and put into the nucleic acid that drives the liquid medicine and draw box 19, another conveyer belt 3 draws box 19 with nucleic acid and send the below of detection mechanism to detect, and then realize drawing, detect the integration, work efficiency has been improved greatly.

Further, the magnetic bead adding mechanism comprises a magnetic bead box 2, the magnetic bead box 2 is fixedly connected with the inner wall of the detection box 1, the inner wall of the magnetic bead box 2 is provided with a first installation opening, the inner walls of two sides of the first installation opening are rotatably connected with a same material blocking convex disc 25 through bearings, the outer wall of the magnetic bead box 2 is fixedly connected with a third motor 26, one end of an output shaft of the third motor 26 is fixedly connected with the material blocking convex disc 25, the first magnetic bead adding mechanism is matched with the first motor 5, and magnetic beads are added in the sample box 8 in sequence.

Further, the liquid medicine adding mechanism comprises a second electric push rod 17, a second mounting opening is formed in the inner wall of one side of the detection box 1, the inner wall of the second mounting opening is fixedly connected with the second electric push rod 17, a medicine adding gun 18 is fixedly connected to one end of a piston rod of the second electric push rod 17, and a proper amount of medicine is sequentially added into the nucleic acid extracting box 19 through the medicine adding gun 18.

Further, the detection mechanism comprises a laser emitter 22, the laser emitter 22 is fixedly connected with the inner wall of the detection box 1, the inner wall of the top of the detection box 1 is provided with a fluorescence detection device 24, and the nucleic acid in the nucleic acid extraction box 19 is subjected to fluorescence detection through the detection mechanism.

Further, the shading subassembly includes two 21 baffles, two 21 baffles and 16 fixed connection of baffle, one side outer wall of two 21 baffles is provided with sliding tray 23, the inner wall sliding connection of sliding tray 23 has light screen 20, the top outer wall fixedly connected with stay cord 15 of light screen 20, the outer wall fixedly connected with take-up reel 14 of the output shaft of two 13 motors, the outer wall and the stay cord 15 fixed connection of take-up reel 14, utilize light screen 20 at the in-process that detects, play the effect that blocks light, and then avoid the interference of external light.

The working principle is as follows:

opening a door on one side of the detection box 1, putting a sample box 8 containing a sample into a limiting sleeve 7, moving the sample box 8 to a position right below a discharge port of a magnetic bead box 2 through a conveyer belt 3, driving a material blocking convex disc 25 to rotate by a motor III 26, further conveying a magnetic bead into the sample box 8, moving the sample box 8 to a position right below a magnetic rod 9 after adding, adsorbing the magnetic bead with nucleic acid onto the magnetic rod 9 by the magnetic rod 9, driving the magnetic rod 9 to horizontally rotate to the position above a nucleic acid extraction box 19 by a motor II 13, putting the magnetic bead into the nucleic acid extraction box 19 containing a medicament, moving the nucleic acid extraction box 19 to a position right below a fluorescence detection device 24 through another conveyer belt 3, driving the magnetic rod 9 to reset by the motor II 13, simultaneously slowly dropping a light shielding plate 20 under the action of a pull rope 15, opening a laser emitter 22, and detecting through the fluorescence detection device 24, after completion of the detection, the door on the other side of the detection chamber 1 is opened, and the nucleic acid extracting cassette 19 is taken out.

It is above only the utility model discloses a preferred embodiment, the utility model discloses a scope of protection does not only confine above-mentioned embodiment, the all belongs to the utility model discloses a technical scheme under the thinking all belongs to the utility model discloses a scope of protection. It should be noted that, for those skilled in the art, various modifications and decorations can be made without departing from the principle of the present invention, and these modifications and decorations should also be regarded as the protection scope of the present invention.

Claims

1. A full-automatic animal epidemic disease nucleic acid extraction and fluorescence PCR detection all-in-one machine is characterized in that: the kit comprises a detection box (1), wherein the inner wall of one opposite side of the detection box (1) is fixedly connected with a first partition plate (16), a conveyor belt (3) is arranged in the detection box (1), the conveyor belt (3) is symmetrically distributed, the outer wall of the top of the conveyor belt (3) is fixedly connected with a fixed disc (4), the outer wall of the top of the fixed disc (4) is fixedly connected with a first motor (5), one end of an output shaft of the first motor (5) is fixedly connected with a rotating disc (6), the outer wall of the top of one rotating disc (6) is fixedly connected with a plurality of limiting sleeves (7), the inner wall of each limiting sleeve (7) is inserted with a sample box (8), the outer wall of the top of the other rotating disc (6) is fixedly connected with a plurality of nucleic acid extraction boxes (19) through buckles, and the outer wall of the top of the detection box (1) is fixedly connected with a second motor (13), output shaft one end fixedly connected with connecting block (12) of motor two (13), the bottom outer wall fixedly connected with electric putter (11) of connecting block (12), the piston rod one end fixedly connected with magnetism frame (10) of electric putter (11), a plurality of magnetism stick (9) of the bottom outer wall fixedly connected with of magnetism frame (10), nucleic acid extraction box (19) and sample box (8) all with magnetism stick (9) looks adaptation, the inside of detection case (1) is provided with detection mechanism, magnetic bead and adds mechanism, liquid medicine and shading subassembly.

2. The full-automatic animal epidemic nucleic acid extraction and fluorescence PCR detection all-in-one machine of claim 1, which is characterized in that: the magnetic bead adds the mechanism and includes magnetic bead box (2), the inner wall fixed connection of magnetic bead box (2) and detection case (1), the inner wall of magnetic bead box (2) is provided with installing port one, the both sides inner wall of installing port is rotated through the bearing and is connected with same material stopping convex disc (25), the outer wall fixedly connected with motor three (26) of magnetic bead box (2), the output shaft one end and the material stopping convex disc (25) fixed connection of motor three (26).

3. The full-automatic animal epidemic nucleic acid extraction and fluorescence PCR detection all-in-one machine of claim 2, characterized in that: the liquid medicine adding mechanism comprises a second electric push rod (17), a second mounting opening is formed in the inner wall of one side of the detection box (1), the inner wall of the second mounting opening is fixedly connected with the second electric push rod (17), and a medicine adding gun (18) is fixedly connected to one end of a piston rod of the second electric push rod (17).

4. The full-automatic animal epidemic nucleic acid extraction and fluorescence PCR detection all-in-one machine of claim 1, which is characterized in that: the detection mechanism comprises a laser emitter (22), the laser emitter (22) is fixedly connected with the inner wall of the detection box (1), and a fluorescence detection device (24) is arranged on the inner wall of the top of the detection box (1).

5. The full-automatic animal epidemic nucleic acid extraction and fluorescence PCR detection all-in-one machine of claim 1, which is characterized in that: the shading assembly comprises a second partition board (21), the second partition board (21) is fixedly connected with the first partition board (16), a sliding groove (23) is formed in the outer wall of one side of the second partition board (21), a shading plate (20) is connected to the inner wall of the sliding groove (23) in a sliding mode, a pull rope (15) is fixedly connected to the outer wall of the top of the shading plate (20), a take-up reel (14) is fixedly connected to the outer wall of an output shaft of the second motor (13), and the outer wall of the take-up reel (14) is fixedly connected with the pull rope (15).