CN216956072U - Immunoreaction and light detection system - Google Patents
Immunoreaction and light detection system Download PDFInfo
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- CN216956072U CN216956072U CN202220206521.2U CN202220206521U CN216956072U CN 216956072 U CN216956072 U CN 216956072U CN 202220206521 U CN202220206521 U CN 202220206521U CN 216956072 U CN216956072 U CN 216956072U
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Abstract
The utility model discloses an immunoreaction and optical detection system, which comprises a first rotary table, a second rotary table, a uniform mixing module, a chemiluminescence detection module, an exciting liquid separate injection module and a phi-axis transmission mechanism, wherein the first rotary table is connected with the second rotary table through a connecting rod; a plurality of station grooves for inserting reaction cups are formed in the first rotating disc and the second rotating disc respectively, and the first rotating disc and the second rotating disc are correspondingly provided with one mixing module; the first rotating disc is used for receiving the reaction cups and the samples and shaking up under the action of the blending module, and the second rotating disc is used for receiving the reaction cups of the first rotating disc and performing operations of adding pre-excitation liquid, shaking up, adding excitation liquid and detecting light; the chemiluminescence detection module and the exciting liquid dispensing module are arranged on the side edge of the second turntable; the phi-axis transmission mechanism is used for driving the first rotating disc and the second rotating disc to rotate. The utility model has compact structure, can realize the optimized system layout and reduce the occupied space.
Description
Technical Field
The utility model relates to the technical field of chemiluminescence immunoassay instruments, in particular to an immunoreaction and light detection system.
Background
Chemiluminescence immunoassay (CLIA) is a detection and analysis technique for various antigens, haptens, antibodies, hormones, enzymes, fatty acids, vitamins, drugs, and the like by combining chemiluminescence measurement technique with high sensitivity and high specificity immunoreaction. Is a latest immunoassay technology developed after radioimmunoassay, enzyme immunoassay, fluoroimmunoassay and time-resolved fluoroimmunoassay.
Chemiluminescent immunoassays comprise two components, an immunoreaction system and a chemiluminescent assay system. The chemiluminescence analysis system is characterized in that chemiluminescence substances are catalyzed by a catalyst and oxidized by an oxidant to form an excited intermediate, when the excited intermediate returns to a stable ground state, photons (hM) are emitted at the same time, and a luminescence quantum yield is measured by a luminescence signal measuring instrument. The immunoreaction system is to label the luminous matter (excited to produce excited state intermediate under the excitation of reactant) directly onto antigen (chemiluminescence immunoassay) or antibody (immunochemiluminescence assay), or to act enzyme on the luminous substrate.
In the existing chemiluminescence immunoassay instrument, the occupied space of an immunoassay system is large, the volume of the whole instrument is also influenced, and the existing chemiluminescence immunoassay instrument has large volume and cannot meet the use requirements of part of customers.
SUMMERY OF THE UTILITY MODEL
The utility model aims to provide an immunoreaction and light detection system, which realizes the optimization of system layout and reduces the space occupation.
In order to achieve the above purpose, the solution of the utility model is:
an immunoreaction and optical detection system comprises a first rotary table, a second rotary table, a uniform mixing module, a chemiluminescence detection module, an exciting liquid separate injection module and a phi-axis transmission mechanism; a plurality of station grooves for inserting reaction cups are formed in the first rotating disc and the second rotating disc respectively, and the first rotating disc and the second rotating disc are correspondingly provided with one mixing module; the first rotating disc is used for receiving the reaction cups and the samples and shaking up under the action of the blending module, and the second rotating disc is used for receiving the reaction cups of the first rotating disc and performing operations of adding pre-excitation liquid, shaking up, adding excitation liquid and detecting light; the chemiluminescence detection module and the exciting liquid dispensing module are arranged on the side edge of the second turntable; the phi-axis transmission mechanism is used for driving the first rotating disc and the second rotating disc to rotate.
The chemiluminescence detection module comprises a light detection component, a heat insulation heating component and a shutter component.
The excited liquid dispensing module comprises a first dispensing component, a second dispensing component, a waste liquid sucking component, a first plate, a shading component and a Z-axis transmission mechanism; the first dispensing component is used for dispensing a pre-excitation liquid, and the second dispensing component is used for dispensing an excitation liquid; the first sub-injection part, the second sub-injection part and the waste liquid suction part are all arranged on the first plate and respectively correspond to a station slot of the second turntable, and the first sub-injection part, the second sub-injection part and the waste liquid suction part are sequentially arranged along the rotating direction of the second turntable; the light shielding component is arranged opposite to the second dispensing component and is used for shielding light after the second dispensing component dispenses; the Z-axis transmission mechanism is used for driving the first plate to move up and down.
The blending module is arranged between the stations corresponding to the first split injection part and the second split injection part.
The shading parts comprise an upper shading part sleeved at the lower end of the second split injection part, a lower shading part positioned below the second turntable and opposite to the upper shading part, a second plate used for mounting the lower shading part, a first spring used for driving the upper shading part to reset downwards and a second spring used for driving the lower shading part to reset upwards; the second plate is driven by the Z-axis transmission mechanism to move up and down.
Z axle drive mechanism adopts the drive mode that hold-in range, guide rail and cam combine, realizes simultaneous control the relative motion of first plate, second plate.
The immunoreaction and light detection system also comprises a uniform mixing support component; the mixing supporting component comprises a third plate and mechanical claws arranged on two sides of the third plate, the mechanical claws are arranged opposite to the station grooves and used for movably gripping the upper end part of the reaction cup to realize fixation.
After the technical scheme is adopted, different work of distribution reaction and light detection is carried out in a double-turntable mode, so that operations such as adding a sample, shaking up, adding pre-excitation liquid, shaking up, adding excitation liquid and light detection are completed, the modules are matched more compactly, the space design is more reasonable, the space occupation is reduced, and the volume design of the whole instrument is smaller.
Drawings
FIG. 1 is a perspective view of an embodiment of the present invention
FIG. 2 is a second perspective view of the present invention;
FIG. 3 is a front view of a portion of the structure of an embodiment of the present invention;
the reference numbers illustrate:
1-a first turntable; 11- -a station groove; 2-a second turntable;
21- -a station tank; 3- -blending module; 4-a chemiluminescent detection module;
41- -light detection means; 42- -thermally insulated heating elements; 43- -shutter member;
5- - -an exciting liquid dispensing module; 51- - -first dispensing member; 52- -a second dispensing component;
53- -waste liquid suction means; 54-a first plate; 55- -a shutter member;
551-upper shade; 552-lower shade; 553- -a second plate;
554-a first spring; 555 — a second spring; 56- -Z-axis transmission;
6- - -shaft transmission mechanism; 7- -a blending support assembly; 71- -a third plate;
72- -gripper; a- - -a reaction cup.
Detailed Description
In order to further explain the technical solution of the present invention, the present invention is explained in detail by the following specific examples.
The utility model relates to an immunoreaction and optical detection system, which comprises a first rotary table 1, a second rotary table 2, a blending module 3, a chemiluminescence detection module 4, an excitation liquid separate injection module 5 and a phi-axis transmission mechanism 6;
a plurality of station grooves 11 and 21 for inserting the reaction cups a are respectively arranged on the first rotating disc 1 and the second rotating disc 2, and a uniformly mixing module 3 is correspondingly arranged on each of the first rotating disc 1 and the second rotating disc 2;
the first rotary table 1 is used for receiving the reaction cups a and the samples and shaking up under the action of the blending module 3, and the second rotary table 2 is used for receiving the reaction cups a of the first rotary table 1 and performing operations of adding pre-excitation liquid, shaking up, adding excitation liquid and detecting light; the chemiluminescence detection module 4 and the excitation liquid dispensing module 5 are both arranged on the side edge of the second turntable 2;
the phi-axis transmission mechanism 6 is used for driving the first rotating disc 1 and the second rotating disc 2 to rotate.
Referring to fig. 1-3, a specific embodiment of the present invention is shown.
The chemiluminescence detection module 4 comprises a light detection component 41, a heat insulation heating component 42 and a shutter component 43, the structure is completely sealed, light leakage is avoided, the shutter component 43 adopts a control mode driven by an electromagnet to control light feeding opportunity, and meanwhile, the heat insulation component is heated to ensure a constant temperature environment.
The excitation liquid dispensing module 5 includes a first dispensing member 51, a second dispensing member 52, a waste liquid suction member 53, a first plate 54, a light shielding member 55, and a Z-axis transmission mechanism 56; a first dispensing member 51 for dispensing a pre-excitation liquid, and a second dispensing member 52 for dispensing an excitation liquid; the first sub-injection part 51, the second sub-injection part 52 and the waste liquid suction part 53 are all arranged on the first plate 54 and respectively correspond to the station groove 21 of one second turntable 2, and the first sub-injection part, the second sub-injection part and the waste liquid suction part are sequentially arranged along the rotating direction of the second turntable 2; a light shielding member 55 provided opposite to the second dispensing member 52 for shielding light after dispensing from the second dispensing member 52; the Z-axis transmission mechanism 56 is used for driving the first plate 54 to perform the lifting movement. With the lowering of the first plate 54, the first dispensing part 51, the second dispensing part 52, and the waste liquid suction part 53 can operate the cuvettes a at three different stations at the same time to improve the efficiency of processing the sample.
In some embodiments of the excitation liquid dispensing module 5, the blending module 3 is disposed between the stations corresponding to the first dispensing member 51 and the second dispensing member 52, that is, after dispensing the pre-excitation liquid, shaking up is performed first, and then dispensing the excitation liquid.
In some embodiments of the aforementioned excitation liquid dispensing module 5, the light shielding member 55 includes an upper light shielding member 551 sleeved on the lower end of the second dispensing member 52, a lower light shielding member 552 located below the second turntable 2 and opposite to the upper light shielding member 551, a second plate 553 for mounting the lower light shielding member 552, a first spring 554 for driving the upper light shielding member 551 to return downward, and a second spring 555 for driving the lower light shielding member 552 to return upward; the second plate 553 is driven to move up and down by the Z-axis transmission mechanism 56. When the light shielding member 55 works, the upper light shielding member 551 and the lower light shielding member 552 respectively contact with the upper surface and the lower surface of the second turntable 2, and the first spring 554 and the second spring 555 play a buffering role, so that the light shielding member 55 presses the second turntable 2 under the elastic force of the first spring and the second spring, thereby avoiding light leakage.
Further, the Z-axis transmission mechanism 56 employs a transmission method combining a timing belt, a guide rail, and a cam, and simultaneously controls the opposite movement of the first plate 54 and the second plate 553, thereby realizing the synchronous operation of the light shielding member 55. Further, the first dispensing member 51 and the second dispensing member 52 are moved in synchronization with each other while light is blocked.
The utility model also comprises a blending support component 7; the blending support component 7 comprises a third plate 71 and mechanical claws 72 arranged on two sides of the third plate 71, wherein the mechanical claws 72 are arranged opposite to the station grooves 11 and 21 and used for movably holding the upper end parts of the reaction cups a to realize fixation, and the blending support component is matched with the blending module 3 to finish the blending operation of the reaction cups a.
The working principle of the utility model is as follows:
the first rotary table 1 receives and distributes cuvettes a sent from a cuvette loading system of the apparatus, and receives and shakes samples (added with reagents) sent from a sample dispensing system; then, under the action of the cuvette loading system, the cuvette a with the sample and the reagent mixed uniformly is transferred to the second turntable 2, and the operations of adding the pre-excitation liquid, shaking up and adding the excitation liquid are performed, and finally, the chemiluminescence reaction is performed and the detection is performed under the cooperation of the light shielding part 55; after the completion of the detection, the waste liquid is sucked by the waste liquid suction part 53 and the used cuvette a is discarded by the cuvette loading system.
Through the scheme, different work of distribution reaction and light detection is carried out in a double-turntable mode to complete operations of adding samples, shaking up, adding pre-excitation liquid, shaking up, adding excitation liquid, detecting light and the like, and the modules are matched more compactly, the space design is more reasonable, the space occupation is reduced, and the volume design of the whole instrument is smaller.
The above embodiments and drawings are not intended to limit the form and style of the present invention, and any suitable changes or modifications thereof by those skilled in the art should be considered as not departing from the scope of the present invention.
Claims (7)
1. An immune response and light detection system, comprising:
the device comprises a first rotating disc, a second rotating disc, a blending module, a chemiluminescence detection module, an exciting liquid separate injection module and a phi-axis transmission mechanism;
a plurality of station grooves for inserting reaction cups are formed in the first rotating disc and the second rotating disc respectively, and the first rotating disc and the second rotating disc are correspondingly provided with one mixing module;
the first rotating disc is used for receiving the reaction cups and the samples and shaking up under the action of the blending module, and the second rotating disc is used for receiving the reaction cups of the first rotating disc and performing operations of adding pre-excitation liquid, shaking up, adding excitation liquid and detecting light; the chemiluminescence detection module and the excitation liquid dispensing module are arranged on the side edge of the second turntable;
the phi-axis transmission mechanism is used for driving the first rotating disc and the second rotating disc to rotate.
2. The system of claim 1, wherein the light source comprises:
the chemiluminescence detection module comprises a light detection component, a heat insulation heating component and a shutter component.
3. The system of claim 1, wherein the light source comprises:
the excited liquid dispensing module comprises a first dispensing component, a second dispensing component, a waste liquid sucking component, a first plate, a shading component and a Z-axis transmission mechanism;
the first dispensing component is used for dispensing a pre-excitation liquid, and the second dispensing component is used for dispensing an excitation liquid;
the first sub-injection component, the second sub-injection component and the waste liquid suction component are all arranged on the first plate and respectively correspond to a station groove of the second turntable, and the first sub-injection component, the second sub-injection component and the waste liquid suction component are sequentially arranged along the rotating direction of the second turntable;
the light shielding component is arranged opposite to the second dispensing component and is used for shielding light after the second dispensing component dispenses;
the Z-axis transmission mechanism is used for driving the first plate to move up and down.
4. The system of claim 3, wherein the light source comprises:
the blending module is arranged between the stations corresponding to the first split injection part and the second split injection part.
5. The system of claim 3, wherein the light source comprises:
the shading parts comprise an upper shading part sleeved at the lower end of the second split injection part, a lower shading part positioned below the second turntable and opposite to the upper shading part, a second plate used for mounting the lower shading part, a first spring used for driving the upper shading part to reset downwards and a second spring used for driving the lower shading part to reset upwards;
the second plate is driven by the Z-axis transmission mechanism to move up and down.
6. The system of claim 5, wherein the light source comprises:
z axle drive mechanism adopts the drive mode that hold-in range, guide rail and cam combine, realizes simultaneous control the relative motion of first plate, second plate.
7. The system of claim 1, wherein the light source comprises:
the mixing device also comprises a mixing support component;
the blending supporting component comprises a third plate and mechanical claws arranged on two sides of the third plate, wherein the mechanical claws and the station grooves are oppositely arranged and are used for movably holding the upper end part of the reaction cup to realize fixation.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202220206521.2U CN216956072U (en) | 2022-01-25 | 2022-01-25 | Immunoreaction and light detection system |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202220206521.2U CN216956072U (en) | 2022-01-25 | 2022-01-25 | Immunoreaction and light detection system |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN216956072U true CN216956072U (en) | 2022-07-12 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202220206521.2U Active CN216956072U (en) | 2022-01-25 | 2022-01-25 | Immunoreaction and light detection system |
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| CN (1) | CN216956072U (en) |
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- 2022-01-25 CN CN202220206521.2U patent/CN216956072U/en active Active
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