CN216891029U

CN216891029U - Novel spacing adherent blake bottle of tissue

Info

Publication number: CN216891029U
Application number: CN202220681302.XU
Authority: CN
Inventors: 潘春雷; 成建平; 丁霞敏; 王军霞; 朱建荣
Original assignee: Genesis Stemcell Regenerative Medicine Engineering Co ltd
Current assignee: Genesis Stemcell Regenerative Medicine Engineering Co ltd
Priority date: 2022-03-28
Filing date: 2022-03-28
Publication date: 2022-07-05
Anticipated expiration: 2032-03-28

Abstract

The utility model provides a novel tissue limit adherent culture bottle, which comprises a tissue culture bottle, a culture bottle bottom plate, culture holes, a bottle mouth, a sealing cover, a limit grid plate and a limit baffle post, wherein the right end of the tissue culture bottle is provided with the bottle mouth, the right side of the annular side surface of the bottle mouth is provided with the sealing cover, the left side of the annular side surface in the sealing cover is provided with a baffle plate, the right side of the baffle plate is provided with a microporous filter membrane, the tissue culture bottle bottom plate is arranged in the tissue culture bottle, the culture holes are formed in the upper end surface of the culture bottle bottom plate, the left side of the edge of the culture holes is provided with the limit grid plate, and the back side of the edge of the culture holes is provided with the limit baffle post. The utility model has reasonable structure, enables the tissue to adhere to the wall quickly, shortens the tissue culture period and reduces the culture cost.

Description

Novel spacing adherent blake bottle of tissue

Technical Field

The utility model relates to a novel tissue limiting adherent culture bottle, belonging to the technical field of bioengineering.

Background

In the process of tissue adherent culture, key factors such as the size of tissue blocks, the inoculation density, the distance between tissue blocks, the inoculation amount of a culture medium and the like influence the indexes such as cell climbing time, the culture period of primary cells, the number and activity of harvested cells and the like. The tissue blocks have uniform size, proper spacing, proper culture medium, easy adherence of tissues, short cell growth period and easy climbing out of the cultured tissue blocks.

However, the conventional culture process using a common cell culture flask to inoculate a tissue block for culture has the following disadvantages:

1. the distance between tissue blocks is not controlled, and the tissue blocks are easily distributed unevenly. In the tissue block dense area, the time for the cells to climb out of the tissue is long, the migration space of the grown cells is limited, the cells are easy to gather, the nutrition supply is insufficient, the cells are easy to age and contact and inhibit, and the quality of the activity of the cells is reduced;

2. the tissue blocks of inoculation are different in size and uneven in distribution, the tissue is easy to stick to the bottom of the bottle, the tissue blocks which are not firm to adhere to the wall can slide down when cells are observed or liquid is changed, the pipette can be blocked by large tissue, and small tissue can be sucked. If the sucked culture solution is sieved or centrifuged to recover tissues again, the cost and risk are increased, and a novel tissue limiting adherent culture bottle is urgently needed to solve the problems.

SUMMERY OF THE UTILITY MODEL

Aiming at the defects in the prior art, the utility model aims to provide a novel tissue limiting adherent culture bottle to solve the problems in the background art, and the tissue limiting adherent culture bottle is reasonable in structure, enables tissues to adhere to the wall rapidly, shortens the tissue culture period and reduces the culture cost.

In order to achieve the purpose, the utility model is realized by the following technical scheme: a novel tissue limit adherent culture bottle comprises a tissue culture bottle, a culture bottle bottom plate, culture holes, a bottle mouth, a sealing cover, a limit grid plate and a limit baffle column, the right end of the tissue culture bottle is provided with a bottle mouth, the right side of the annular side surface of the bottle mouth is provided with a sealing cover, a baffle is arranged on the left side of the inner annular side surface of the sealing cover, a microporous filter membrane is arranged on the right side of the baffle, a culture bottle bottom plate is arranged in the tissue culture bottle, a culture hole is arranged in the upper end surface of the culture bottle bottom plate, the left side of the edge of the culture hole is provided with a limiting grid plate, the rear side of the edge of the culture hole is provided with a limiting baffle column, the left side of the annular side surface of the tissue culture bottle is provided with a limit baffle, the left end of the tissue culture bottle is assembled with an end head clamping cover, the end clamp cover is internally provided with a rubber sealing base plate, and the left end face of the end clamp cover is provided with a shifting piece.

Further, the bottom plate of the culture bottle is a thick solid plastic plate, and the culture holes are arranged in a bowl-shaped concave structure.

Further, the bottom plate and the culture hole of the culture bottle are processed by TC.

Furthermore, the culture holes are provided with a plurality of groups, the diameters of the culture holes are the same, and the culture holes are uniformly arranged in the upper end face of the bottom plate of the culture bottle.

Furthermore, the limiting grid plate and the limiting blocking column are arranged at the opening of the edge of the culture hole in a right-angle distribution structure.

Furthermore, the outer annular side of the bottle nozzle is provided with an external thread, the inner annular side of the sealing cover is provided with an internal thread, and the sealing cover is hermetically connected with the bottle nozzle through the thread.

Further, the grooves are formed in the upper end face and the lower end face of the shifting piece and are of arc structures, right-angle grooves are formed in the edge positions of the rubber sealing gasket plates, the height of each right-angle groove is the same as the wall thickness of the tissue culture bottle, and the end clamping covers and the tissue culture bottles are of detachable structures.

Furthermore, the separation blade sets up to ring annular structure, the separation blade internal diameter is the same with the bottleneck diameter, sealed lid right-hand member face is inside to be seted up multiunit bleeder vent.

The utility model has the beneficial effects that: the novel tissue limit adherent culture bottle provided by the utility model is added with a culture bottle bottom plate, culture holes, a sealing cover, a limit grid plate, a limit stop post, an end clamping cover, a limit baffle, a rubber sealing base plate and a microporous filter membrane, and has the beneficial effects that:

1. the tissue blocks are independent from each other, contact adhesion does not occur, cells obtained from each tissue can grow radially and adherently independently, and stacking aging does not occur.

2. In the process of culturing, when changing liquid operation, the difficult emergence displacement of the tissue piece in each cultivation cave shortens the tissue adherence cycle, improves and cultivates efficiency, and the tissue piece can not drop and block up the pipette and influence the liquid operation of changing, has improved the convenience of operation.

Drawings

Other features, objects and advantages of the utility model will become more apparent upon reading of the detailed description of non-limiting embodiments with reference to the following drawings:

FIG. 1 is a schematic structural view of a novel tissue-limiting adherent culture flask according to the utility model;

FIG. 2 is a schematic view of a local structure of a culture cavity in a novel tissue-limiting adherent culture bottle according to the utility model;

FIG. 3 is a front cross-sectional view of a novel tissue-limiting adherent culture flask of the present invention;

FIG. 4 is an enlarged schematic view of the structure of A in a novel tissue-limiting adherent culture flask of the present invention;

in the figure: 1-tissue culture bottle, 2-culture bottle bottom plate, 3-culture hole, 4-bottle mouth, 5-sealing cover, 6-limiting grid plate, 7-limiting baffle column, 8-end clamping cover, 9-limiting baffle plate, 10-rubber sealing backing plate, 11-shifting sheet, 12-baffle sheet, 13-microporous filter membrane, 51-air hole and 111-groove.

Detailed Description

In order to make the technical means, the creation characteristics, the achievement purposes and the effects of the utility model easy to understand, the utility model is further described with the specific embodiments.

Referring to fig. 1-4, the present invention provides a technical solution: a novel tissue limit adherent culture bottle comprises a tissue culture bottle 1, a culture bottle bottom plate 2, culture holes 3, a bottle mouth 4, a sealing cover 5, a limit grid plate 6 and a limit baffle post 7, wherein the right end of the tissue culture bottle 1 is provided with the bottle mouth 4, the right side of the annular side surface of the bottle mouth 4 is provided with the sealing cover 5, the left side of the annular side surface in the sealing cover 5 is provided with a baffle plate 12, the right side of the baffle plate 12 is provided with a microporous filter membrane 13, the tissue culture bottle 1 is internally provided with the culture bottle bottom plate 2, the upper end surface of the culture bottle bottom plate 2 is internally provided with the culture holes 3, the left side of the edge of the culture holes 3 is provided with the limit grid plate 6, the rear side of the edge of the culture holes 3 is provided with the limit baffle post 7, the left side of the annular side surface of the tissue culture bottle 1 is provided with a limit baffle plate 9, the left end of the tissue culture bottle 1 is provided with an end head clamping cover 8, the inside of which is provided with a rubber sealing cushion plate 10, the left end head clamping cover 8 is provided with a shifting plate 11, the design can ensure that tissue blocks are protected independently without contact adhesion, cells obtained by each tissue can grow in an independent radial adherence manner without stacking aging, and meanwhile, the tissue inoculation density can be controlled accurately, and more accurate flow data is provided for experiments and production.

The bottom plate 2 of the culture bottle is a thick solid plastic plate, the culture holes 3 are arranged in a bowl-shaped concave structure, the size, the inoculation density and the inoculation position of the tissue block can be conveniently controlled in the inoculation stage, and meanwhile, the production cost is reduced.

The culture bottle bottom plate 2 and the culture holes 3 are processed by TC, and the design is convenient for promoting the adherent growth of cells.

Cultivate cave 3 and be provided with the multiunit, 3 diameters of cave are the same for the multiunit, and 3 even setings in the blake bottle bottom plate 2 up end are cultivated to the multiunit that the cave 3 was cultivated to the multiunit that this design is through setting up are convenient for control the inoculation density of tissue piece to in the later stage trades liquid and observation operation, can make the tissue piece remain in the normal position, reduce the interference to tissue piece stability.

Spacing grid tray 6 is right angle distribution structure with spacing bumping post 7 and sets up in 3 edge openings of cultivation cave, and this design can control the distance between the tissue piece, makes to protect between the tissue piece independently mutually, does not take place the contact adhesion.

The outer annular side of mouth of a bottle 4 has seted up the external screw thread, and the internal thread has been seted up to annular side in the sealed lid 5, and sealed lid 5 passes through screw thread sealing connection with mouth of a bottle 4, and this design has improved the leakproofness of junction through threaded connection, carries out on-off operation to sealed lid 5 through the convenience.

Both ends face is inside all to be seted up fluted 111 about plectrum 11, and recess 111 sets up to the arc structure, right angle groove has been seted up to rubber seal backing plate 10 border position, and right angle groove height is the same with 1 wall thickness of tissue culture bottle, the arc recess 111 that should set up is convenient for the staff through the setting carries out the push-and-pull operation to plectrum 11 in this design, the antiskid nature of plectrum 11 has been improved, simultaneously through the right angle groove that sets up be convenient for make tissue culture bottle 1 and rubber seal backing plate 10 carry out airtight laminating, the leakproofness of junction has been improved, end card cover 8 is detachable construction with tissue culture bottle 1, this design can be dismantled so that place culture bottle bottom plate 2 inside tissue culture bottle 1 to end card cover 8.

Separation blade 12 sets up to ring annular structure, and separation blade 12 internal diameter is the same with the 4 diameters of bottleneck, and this design is convenient for carry out spacing fixed to bottleneck 4 through separation blade 12, and inside the 5 right-hand members face of sealed lid was seted up to multiunit bleeder vent 51, and this design is convenient for tissue culture and the external gas exchange that carries out through the multiunit bleeder vent 51 that sets up.

As an embodiment of the present invention: firstly, according to the specification of tissue blocks required by culture, selecting a tissue culture bottle 1 with a matched diameter, depth and density of a culture hole 3, crushing the tissue blocks to a required size, adding a proper amount of culture medium, uniformly blowing the tissue blocks by using a pipette, inoculating the tissue blocks into the tissue culture bottle 1, shaking the tissue culture bottle 1 to enable the tissue blocks with the proper size to fall into the culture hole 3, enabling overlarge tissue blocks not to fall into the culture hole 3, enabling undersize tissue blocks to float in the culture medium, continuing shaking the tissue culture bottle 1 to enable the tissue blocks not falling into the culture hole 3 to be gathered to one side of the tissue culture bottle 1, inclining the tissue culture bottle 1 to one side of a limiting grid plate 6, sucking out the redundant tissue blocks, inclining the tissue culture bottle 1 to one side of the limiting grid plate 6 during liquid change, sucking out old culture medium and adding new culture medium.

While there have been shown and described what are at present considered the fundamental principles and essential features of the utility model and its advantages, it will be apparent to those skilled in the art that the utility model is not limited to the details of the foregoing exemplary embodiments, but is capable of other specific forms without departing from the spirit or essential characteristics thereof. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the utility model being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein. Any reference sign in a claim should not be construed as limiting the claim concerned.

Furthermore, it should be understood that although the present description refers to embodiments, not every embodiment may contain only a single embodiment, and such description is for clarity only, and those skilled in the art should integrate the description, and the embodiments may be combined as appropriate to form other embodiments understood by those skilled in the art.

Claims

1. The utility model provides a novel spacing adherent blake bottle of tissue, includes tissue blake bottle, blake bottle bottom plate, cultivates cave, bottle neck, sealed lid, spacing grid tray and spacing bumping post, its characterized in that: the tissue culture bottle right-hand member is provided with the bottle neck, bottle neck annular side right side is equipped with sealed lid, sealed lid inner annular side left side is provided with the separation blade, the separation blade right side is provided with millipore filtration, the inside blake bottle bottom plate that is provided with of tissue culture bottle, the inside cultivation cave of having seted up of blake bottle bottom plate up end, it is provided with spacing grid tray to cultivate cave edge left side, it is provided with spacing bumping post to cultivate cave edge rear side, tissue culture bottle annular side left side is provided with limit baffle, the tissue culture bottle left end is equipped with the end card cover, the inside rubber seal backing plate that is provided with of end card cover, the plectrum is installed to end card cover left end face.

2. The novel tissue-limiting adherent culture flask of claim 1, wherein: the bottom plate of the culture bottle is a thick solid plastic plate, and the culture holes are of bowl-shaped concave structures.

3. The novel tissue-limiting adherent culture flask of claim 1, wherein: the bottom plate of the culture bottle and the culture holes are processed by TC.

4. The novel tissue-limiting adherent culture flask of claim 1, wherein: the culture holes are provided with a plurality of groups, the diameters of the culture holes are the same, and the culture holes are uniformly arranged in the upper end surface of the bottom plate of the culture bottle.

5. The novel tissue-limiting adherent culture flask of claim 1, wherein: the limiting grid plate and the limiting stop pillar are arranged at the opening of the edge of the culture hole in a right-angle distribution structure.

6. The novel tissue-limiting adherent culture flask of claim 1, wherein: the outer annular side of the bottle nozzle is provided with an external thread, the inner annular side of the sealing cover is provided with an internal thread, and the sealing cover is hermetically connected with the bottle nozzle through the thread.

7. The novel tissue-limiting adherent culture flask of claim 1, wherein: the utility model discloses a tissue culture bottle, including stirring piece, end card cover, groove, rubber sealing gasket, right-angle groove, tissue culture bottle wall thickness, the both ends face is inside all seted up flutedly about the stirring piece, just the groove sets up to arc structure, right-angle groove has been seted up to rubber sealing gasket flange edge position, and right-angle groove height is the same with tissue culture bottle wall thickness, the end card cover is detachable construction with tissue culture bottle.

8. The novel tissue-limiting adherent culture flask of claim 1, wherein: the separation blade sets up to ring annular structure, the separation blade internal diameter is the same with the bottleneck diameter, sealed lid right-hand member face is inside to be seted up multiunit bleeder vent.