CN216837911U - Microorganism separation and purification device - Google Patents

Microorganism separation and purification device Download PDF

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Publication number
CN216837911U
CN216837911U CN202220423608.5U CN202220423608U CN216837911U CN 216837911 U CN216837911 U CN 216837911U CN 202220423608 U CN202220423608 U CN 202220423608U CN 216837911 U CN216837911 U CN 216837911U
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separation
purification
mounting
microbial
mounting structure
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CN202220423608.5U
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Chinese (zh)
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张智彧
司同
庞任维
蓝云泉
乔龙江
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Shenzhen Institute of Advanced Technology of CAS
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Shenzhen Institute of Advanced Technology of CAS
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Abstract

The utility model discloses a microorganism separation and purification device, include: the connecting structure can be grabbed by a mechanical arm of the automatic pipetting station; the mounting structure is connected with the connecting structure and is provided with a plurality of separation and purification tools, and the mechanical arm can drive the separation and purification tools to simultaneously perform separation and purification operations; a support structure on which the mounting structure is supported in a state in which the connection structure is separated from the robot arm. The utility model discloses a microbial separation and purification device can utilize the arm of automatic liquid-transfering station to realize the automation of microbial separation and purification operation, and is efficient to can avoid the experimenter to produce the problem that tired sense caused experimental error because of repetitive operation.

Description

Microorganism separation and purification device
Technical Field
The utility model relates to a microorganism experimental facilities technical field especially relates to a microorganism separation and purification device.
Background
The microorganism separation and purification is to obtain a monoclonal colony by a microorganism sample through a solid agar culture medium plating operation, and belongs to a key process in microorganism research. The traditional microorganism separation and purification operation needs to be realized through steps of liquid transfer, coating and the like, and at present, technicians mainly hold a coating rod to move back and forth for coating, so that the standardization level is low; if a large number of samples need to be applied, it is time consuming and the technician is fatigued, often with mistakes. Therefore, the microorganism separation and purification is a complicated experimental link with more repetitive work, and common methods for separating pure microorganisms include a scribing separation method, a gradient dilution coating method and the like, which usually consume a great amount of time for experimental technicians, and have low flux and large experimental error.
SUMMERY OF THE UTILITY MODEL
The utility model aims at providing a little bioseparation purification device to solve present bioseparation purification operation and need consume a large amount of times of experiment technical staff, the flux is low and the big technical problem of experimental error.
The above object of the present invention can be achieved by the following technical solutions:
the utility model provides a microorganism separation and purification device, include: the connecting structure can be grabbed by a mechanical arm of the automatic pipetting station; the mounting structure is connected with the connecting structure and is provided with a plurality of separation and purification tools, and the mechanical arm can drive the separation and purification tools to simultaneously perform separation and purification operations; a support structure on which the mounting structure is supported in a state in which the connection structure is separated from the robot arm.
The utility model discloses an in the embodiment, mounting structure includes the mounting panel, a plurality of mounting holes have been arranged at the interval on the mounting panel, the both ends of separation and purification instrument are operating portion and installation department respectively, the separation and purification instrument is worn to locate in the mounting hole, the operating portion is arranged in the below of mounting hole, the installation department card is in the top of mounting hole.
The utility model discloses an among the embodiment, the pure industry utensil of separation can be followed the mounting hole slides and sets up.
The utility model discloses an among the embodiment, automatic arm that moves liquid station is equipped with a plurality of spliced poles, connection structure includes the piston board, be equipped with a plurality of jacks on the piston board, the jack is the same with the cartridge open-ended shape of liquid-transfering suction head, and is a plurality of the spliced pole can be with a plurality of the jack is pegged graft mutually.
In an embodiment of the present invention, the piston plate is detachably connected to be disposed above the mounting structure.
The utility model discloses an in the embodiment, bearing structure includes the supporting seat, the holding chamber has in the supporting seat, the top of supporting seat has the opening, mounting structure is supported the top of supporting seat, and is a plurality of separation and purification instrument is followed the opening extends to in the holding chamber.
The utility model discloses an among the embodiment, the top of supporting seat is equipped with the spacing groove, mounting structure's bottom is equipped with spacing boss, spacing boss with the spacing groove phase-match.
In an embodiment of the utility model, the separation and purification instrument includes the instrument main part, the one end of instrument main part with operation portion threaded connection, the other end of instrument main part with the installation department is connected.
In an embodiment of the present invention, the operation portion is a coating head.
In an embodiment of the present invention, the operation portion is a dot matrix needle.
The utility model discloses a characteristics and advantage are:
the utility model discloses a microorganism separation and purification device is through installing a plurality of separation and purification instruments on mounting structure to be connected this mounting structure with connection structure, and then snatch this connection structure through the arm of automatic liquid-transfering station, thereby drive a plurality of separation and purification instruments through the arm and separate the purification operation simultaneously, and before connection structure is snatched and after the separation and purification operation is accomplished, support mounting structure through bearing structure, consequently, the utility model discloses a microorganism separation and purification device can utilize the arm of automatic liquid-transfering station to realize the automation of microorganism separation and purification operation, and is efficient, and can avoid the experimenter to cause the problem of experimental error because repetitive operation produces fatigue.
Drawings
In order to more clearly illustrate the technical solutions in the embodiments of the present invention, the drawings needed to be used in the description of the embodiments will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art to obtain other drawings without creative efforts.
FIG. 1 is a perspective view of the microorganism separation and purification apparatus of the present invention.
FIG. 2 is a front view of the microorganism separation and purification apparatus of the present invention.
FIG. 3 is a side view of the microorganism separation and purification apparatus of the present invention.
FIG. 4 is an internal structure view of the microorganism separation and purification apparatus of the present invention.
Fig. 5 is a schematic structural diagram of the piston plate of the present invention.
Fig. 6 is a schematic structural diagram of the mounting plate of the present invention.
Fig. 7 is a use state diagram of the mounting plate of the present invention.
Fig. 8 is a schematic structural view of the support seat of the present invention.
Fig. 9 is a schematic structural view of the tool body of the present invention.
Fig. 10 is a schematic view of the installation of the coating head on the tool body of the present invention.
Fig. 11 is an installation schematic diagram of the dot matrix needle installed on the tool main body of the present invention.
In the figure:
1. a mounting structure; 11. mounting a plate; 12. mounting holes; 13. a limiting boss; 2. a connecting structure; 21. a piston plate; 22. a jack; 3. a support structure; 31. a supporting seat; 32. an opening; 33. an accommodating cavity; 34. a limiting groove; 4. separation and purification means; 41. a tool body; 411. an external thread surface; 42. an installation part; 43. an operation section; 5. coating heads; 6. and (5) dot matrix needles.
Detailed Description
The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.
As shown in fig. 1, the utility model provides a microorganism separation and purification device, which comprises: the connecting structure 2 can be grabbed by a mechanical arm of the automatic pipetting station; the mounting structure 1 is connected with the connecting structure 2, the mounting structure 1 is provided with a plurality of separation and purification tools 4, and the mechanical arm can drive the separation and purification tools 4 to simultaneously perform separation and purification operations; and a support structure 3 on which the mounting structure 1 is supported in a state where the connection structure 2 is separated from the robot arm 3.
The utility model discloses a microorganism separation and purification device is through installing a plurality of separation and purification instruments 4 on mounting structure 1, and be connected this mounting structure 1 with connection structure 2, and then snatch this connection structure 2 through the arm of automatic liquid-transfering station, thereby drive a plurality of separation and purification instruments 4 through the arm and separate the purification operation simultaneously, and before connection structure 2 is snatched and after the separation and purification operation is accomplished, support structure 1 through bearing structure 3, consequently, the utility model discloses a microorganism separation and purification device can utilize the arm of automatic liquid-transfering station to realize the automation of microorganism separation and purification operation, and is efficient to can avoid the experimenter to produce the problem that tired sense caused the experimental error because of repetitive operation. In addition, other mechanical arms and control devices are not required, the cost is low, and the implementation is easy.
In the embodiment, a plurality of liquid-transferring suction heads are grabbed by a mechanical arm of an automatic liquid-transferring station to suck a certain amount of bacteria liquid, and the bacteria liquid is transferred to a culture medium flat plate to be subjected to separation and purification operation, so that the liquid-transferring operation is completed; then, the connecting structure 2 is grabbed by a mechanical arm of the automatic liquid transfer station, the mounting structure 1 is separated from the supporting structure, and then a plurality of separation and purification tools 4 are driven to simultaneously perform microorganism separation and purification operations, so that the bacterial liquid is uniformly distributed on the culture medium flat plate, and the microorganism separation and purification operations are completed; finally the robot arm places the mounting structure 1 on the support structure 3 and releases the connecting structure 2. Therefore, the utility model discloses a microbial separation and purification device utilizes the automation that the arm of automatic liquid moving station realized microbial separation and purification operation, neither can influence this arm and move the liquid operation, can also make simultaneously move the liquid operation step and link up better between the microbial separation and purification operation step.
Optionally, the mechanical arm of the automatic liquid transfer station first grabs the connecting structure 2, so that the mounting structure 1 is separated from the supporting structure, the plurality of separation and purification tools 4 are driven to simultaneously dip a certain amount of bacteria liquid or pick single microbial cells, and then the plurality of separation and purification tools 4 are driven to simultaneously uniformly distribute the dipped bacteria liquid on the culture medium flat plate or transfer the picked single microbial cells on the culture medium flat plate, so that the microorganism separation and purification operation is completed; finally the robot arm places the mounting structure 1 on the support structure 3 and releases the attachment structure 2.
As shown in fig. 1, fig. 2 and fig. 3, specifically, the arm of automatic liquid-transfering workstation has 96 passageways, can snatch 96 liquid-transfering suction head simultaneously and carry out the liquid-transfering operation, therefore, the utility model discloses a microbial separation purification device, install 96 separation and purification instruments 4 on the mounting structure 1, 12 separation and purification instruments 4 are arranged into one row along the length direction interval of mounting structure 1, and 8 rows of separation and purification instruments 4 are arranged along the width direction interval of mounting structure 1, and the arm through automatic liquid-transfering workstation drives 96 separation and purification instruments 4 simultaneously and carries out microbial separation and purification operation.
As shown in fig. 1, 4 and 6, in the embodiment of the present invention, the mounting structure 1 includes a mounting plate 11, a plurality of mounting holes 12 are arranged on the mounting plate 11 at intervals, the two ends of the separation and purification tool 4 are respectively an operation portion 43 and an installation portion 42, the separation and purification tool 4 is inserted into the mounting holes 12, the operation portion 43 is arranged below the mounting holes 12, and the installation portion 42 is clamped above the mounting holes 12. Simple structure and convenient installation.
Specifically, the operation part 43 is one end of the separation and purification tool 4 that contacts with the microorganism to perform the microorganism separation and purification operation. The mounting portion 42 is the end of the separation and purification tool 4 connected to the mounting structure 1. The separation and purification tool 4 includes a tool body 41, an operation portion 43, and a mounting portion 42, and the diameter of the tool body 41 is slightly smaller than the diameter of the mounting hole 12. The diameters of the mounting portion 42 and the operating portion 43 are larger than the diameter of the mounting hole 12, the operating portion 43 is detachably connected to the bottom end of the tool body 41, and the mounting portion 42 is connected to the top end of the tool body 41. The bottom end of the tool body 41 is inserted through the mounting hole 12 and placed below the mounting hole 12, and the operating portion 43 is mounted on the bottom end of the tool body 41.
Optionally, the separation and purification tool comprises a tool body, an operation part and a mounting part, and the diameter of the tool body is slightly smaller than that of the mounting hole. The diameter of installation department and operation portion all is greater than the diameter of mounting hole, and this installation department is connected with the top detachably of this tool body, and the operation portion is connected with the bottom of this tool body. The top end of the tool main body penetrates through the lower part of the mounting hole and is arranged above the mounting hole, and then the mounting part is mounted at the top end of the tool main body, so that the mounting of the separation and purification tool is completed. Optionally, the separation and purification tool is an integrally formed structure, and the diameter of the operation portion is smaller than that of the installation hole, and the diameter of the installation portion is larger than that of the installation hole. And the operation part passes through the mounting hole from the upper part of the mounting hole and is arranged below the mounting hole, so that the installation of the separation and purification tool is completed.
As shown in fig. 4 and 10, in the present embodiment, the operation unit 43 of the separation and purification tool 4 is the coating head 5. The bacterial suspension is applied to the medium plate by the applicator head 5 and uniformly dispersed. One end of the tool body 41 is screwed to the applicator head 5. By screwing the coating head 5 to the tool body 41, the connection is firm and the replacement is facilitated.
In another embodiment, as shown in fig. 4 and 11, the operation part 43 of the separation and purification tool 4 is a dot matrix needle 6. One end of the tool body 41 is screwed to the dot matrix needle 6. By screwing the dot matrix needle 6 with the tool body 41, the connection is firm and the replacement is convenient. The mechanical arm through automatic liquid-transfering station drives a plurality of dot matrix needles 6 and dips in and get the fungus liquid, drives a plurality of dot matrix needles 6 simultaneously and will dip in the fungus liquid dot matrix of getting on the culture medium flat board to accomplish the microorganism separation purification operation.
Specifically, as shown in fig. 9, the bottom end of the tool body 41 has an external thread surface 411, and a suitable operation part 43, such as the coating head 5 or the dot matrix needle 6, is mounted on the tool body 41 by screwing according to the need (such as a culture medium plate of different specifications). Optionally, other detachable connection modes such as a buckle and a clamp can be adopted between the operation part and the tool main body.
As shown in fig. 1, 4, 6 and 7, in the embodiment of the present invention, the separation and purification tool 4 can be slidably disposed along the mounting hole 12. When the separation and purification tool 4 is brought into contact with the medium plate and the separation and purification operation is performed, the separation and purification tool 4 can be adaptively slid along the mounting hole 12 without being inserted into the medium plate. Specifically, the separation and purification means 4 has a slidable space above it. The connection structure 2 is located above the mounting structure 1, and the mounting portion 42 of the separation and purification tool 4 has a certain gap with the connection structure 2, so that the separation and purification tool 4 can slide along the mounting hole 12. Optionally, an elastic buffer structure is arranged between the separation and purification tool and the connecting structure, so that the separation and purification tool can adaptively slide along the mounting hole.
As shown in fig. 4 and 5, in the embodiment of the present invention, the mechanical arm of the automatic pipetting station is provided with a plurality of connecting posts, the connecting structure 2 includes a piston plate 21, a plurality of insertion holes 22 are provided on the piston plate 21, the insertion holes 22 are the same as the shape of the insertion opening of the pipetting tip, and the plurality of connecting posts can be inserted into the plurality of insertion holes 22. The arm of automatic move liquid station is pegged graft mutually through a plurality of spliced poles and a plurality of pipetting tip's cartridge opening to realize snatching of a plurality of pipetting tips, the utility model discloses a set up piston plate 21 to set up a plurality of jacks 22 the same with pipetting tip's cartridge open-ended shape on piston plate 21, thereby can use with the arm of automatic move liquid station is supporting.
The piston plate 21 is arranged above the mounting structure 1 in a detachable connection. The piston plate 21 is removed to facilitate the removal and installation of the plurality of separation and purification tools 4 from the mounting structure 1. Specifically, the piston plate 21 is connected to the mounting structure 1 by a connector, a snap, a clip, or other detachable connection.
As shown in fig. 1, 4 and 8, in the embodiment of the present invention, the supporting structure 3 includes a supporting seat 31, a containing cavity 33 is provided in the supporting seat 31, an opening 32 is provided at the top of the supporting seat 31, the mounting structure 1 is supported at the top of the supporting seat 31, and the plurality of separation and purification tools 4 extend from the opening 32 to the containing cavity 33. The supporting seat 31 supports the mounting structure 1 and extends the separation and purification tools 4 into the accommodating cavity 33, so that the separation and purification tools 4 can be prevented from contacting other objects before operation and being polluted. Specifically, the supporting base 31 is substantially a frame structure. The size of the receiving chamber 33 is not particularly limited, but the operation portion 43 of the separation and purification tool 4 is suspended in the receiving chamber 33 so as not to contact with the lower stage and the support base 31.
As shown in fig. 4, 7 and 8, the top of the supporting seat 31 is provided with a limiting groove 34, the bottom of the mounting structure 1 is provided with a limiting boss 13, and the limiting boss 13 is matched with the limiting groove 34. The mounting structure 1 does not slide on the support base 31 in the longitudinal direction and the width direction of the support base 31 to cause the separation and purification tool 4 to be deflected.
The above description is only for the embodiments of the present invention, and those skilled in the art can make various changes or modifications to the embodiments of the present invention according to the disclosure of the application document without departing from the spirit and scope of the present invention.

Claims (10)

1. A microbial separation and purification device, comprising:
the connecting structure can be grabbed by a mechanical arm of the automatic pipetting station;
the mounting structure is connected with the connecting structure and is provided with a plurality of separation and purification tools, and the mechanical arm can drive the separation and purification tools to simultaneously perform separation and purification operations;
a support structure on which the mounting structure is supported in a state in which the connection structure is separated from the robot arm.
2. The microbial separation and purification device according to claim 1,
mounting structure includes the mounting panel, a plurality of mounting holes have been arranged at the interval on the mounting panel, the both ends of separation and purification instrument are operating portion and installation department respectively, separation and purification instrument wears to locate in the mounting hole, the operating portion is arranged in the below of mounting hole, the installation department card is in the top of mounting hole.
3. The microbial separation and purification apparatus according to claim 2,
the separation purification tool can slide along the mounting hole.
4. The microbial separation and purification device according to claim 1,
the mechanical arm of the automatic pipetting station is provided with a plurality of connecting columns, the connecting structure comprises a piston plate, a plurality of jacks are arranged on the piston plate, the jacks are the same as the shape of a plug-in opening of the pipetting head, and the connecting columns can be connected with the jacks in an inserting mode.
5. The microbial separation and purification apparatus according to claim 4,
the piston plate is detachably connected to be arranged above the mounting structure.
6. The microbial separation and purification device according to claim 1,
the supporting structure comprises a supporting seat, an accommodating cavity is formed in the supporting seat, an opening is formed in the top of the supporting seat, the mounting structure is supported on the top of the supporting seat, and the separation and purification tools extend into the accommodating cavity from the opening.
7. The microbial separation and purification apparatus according to claim 6,
the top of supporting seat is equipped with the spacing groove, mounting structure's bottom is equipped with spacing boss, spacing boss with the spacing groove phase-match.
8. The microbial separation and purification apparatus according to claim 2,
the separation and purification tool comprises a tool main body, one end of the tool main body is in threaded connection with the operation part, and the other end of the tool main body is connected with the installation part.
9. The microbial separation and purification device according to claim 8,
the operation part is a coating head.
10. The microorganism separation and purification apparatus according to claim 8,
the operating part is a dot matrix needle.
CN202220423608.5U 2022-02-28 2022-02-28 Microorganism separation and purification device Active CN216837911U (en)

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CN202220423608.5U CN216837911U (en) 2022-02-28 2022-02-28 Microorganism separation and purification device

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Application Number Priority Date Filing Date Title
CN202220423608.5U CN216837911U (en) 2022-02-28 2022-02-28 Microorganism separation and purification device

Publications (1)

Publication Number Publication Date
CN216837911U true CN216837911U (en) 2022-06-28

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CN202220423608.5U Active CN216837911U (en) 2022-02-28 2022-02-28 Microorganism separation and purification device

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2024082134A1 (en) * 2022-10-18 2024-04-25 中国科学院深圳先进技术研究院 Sampling device for high-throughput preparation of microorganism sample for mass spectrometry

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2024082134A1 (en) * 2022-10-18 2024-04-25 中国科学院深圳先进技术研究院 Sampling device for high-throughput preparation of microorganism sample for mass spectrometry

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