WO2023159577A1

WO2023159577A1 - Separation and purification apparatus for microorganisms

Info

Publication number: WO2023159577A1
Application number: PCT/CN2022/078331
Authority: WO
Inventors: 张智彧; 司同; 庞任维; 蓝云泉; 乔龙江
Original assignee: 深圳先进技术研究院
Priority date: 2022-02-28
Filing date: 2022-02-28
Publication date: 2023-08-31

Abstract

Provided is a separation and purification apparatus for microorganisms, comprising: a connection structure (2) capable of being gripped by a robotic manipulator of an automatic pipetting station; a mounting structure (1) connected to the connecting structure (2), wherein a plurality of separation and purification tools (4) are mounted on the mounting structure (1), and the robotic manipulator can drive the plurality of separation and purification tools (4) to perform separation and purification operations at the same time; and a supporting structure (3), wherein the mounting structure (1) is supported on the supporting structure (3) in a state where the connection structure (2) is separated from the robotic manipulator. The separation and purification apparatus for microorganisms can achieve the automation of separation and purification operations for microorganisms by utilizing the robotic manipulator of the automatic pipetting station, which is highly efficient and can avoid the problem of the experimental error caused by experimenters when they are fatigued due to repeated operations.

Description

Microbial separation and purification device

technical field

The present application relates to the technical field of microbial experiment equipment, in particular, to a microorganism separation and purification device.

Background technique

Microbial isolation and purification is to obtain monoclonal colonies by plating microbial samples on solid agar medium, which is a key process in microbial research. The traditional separation and purification of microorganisms needs to be carried out through steps such as pipetting and coating. At present, technicians mainly use coating rods to move back and forth for coating, and the standardization level is low; if a large number of samples need to be coated, it is very time-consuming and technically People are prone to fatigue and tend to make mistakes. It can be seen from this that the separation and purification of microorganisms is a tedious and repetitive work in an experimental link. The methods usually used to separate pure microorganisms include streak separation method, gradient dilution coating method, etc., which often require a lot of experimental technicians. time, the throughput is low and the experimental error is large.

Contents of the invention

The purpose of this application is to provide a microorganism separation and purification device to solve the technical problems that the current microorganism separation and purification operation needs to consume a lot of time for experimental technicians, the throughput is low and the experimental error is large.

The above-mentioned purpose of the present application can adopt following technical scheme to realize:

The present application provides a microorganism separation and purification device, including: a connection structure, which can be grasped by a mechanical arm of an automatic liquid transfer station; an installation structure, which is connected to the connection structure, and the installation structure is equipped with multiple separation and purification tools. The mechanical arm can drive multiple separation and purification tools to perform separation and purification operations at the same time; the support structure, the installation structure is supported on the support structure when the connection structure is separated from the mechanical arm.

The features and advantages of this application are:

In the microorganism separation and purification device of the present application, a plurality of separation and purification tools are installed on the installation structure, and the installation structure is connected with the connection structure, and then the connection structure is grasped by the mechanical arm of the automatic liquid transfer station, so that the mechanical The arm drives multiple separation and purification tools to perform separation and purification operations at the same time, and before the connection structure is grasped and after the separation and purification operation is completed, the installation structure is supported by the support structure. Therefore, the microbial separation and purification device of the present application can use automatic The mechanical arm of the pipetting station realizes the automation of microorganism separation and purification operation, which has high efficiency and can avoid the problem of experimental errors caused by fatigue caused by repeated operations.

Description of drawings

In order to more clearly illustrate the technical solutions in the embodiments of the present application, the drawings that need to be used in the description of the embodiments will be briefly introduced below. Obviously, the drawings in the following description are only some embodiments of the present application. For those skilled in the art, other drawings can also be obtained based on these drawings without creative effort.

Fig. 1 is a perspective view of the microorganism separation and purification device of the present application.

Fig. 2 is a front view of the microorganism separation and purification device of the present application.

Fig. 3 is a side view of the microorganism separation and purification device of the present application.

Fig. 4 is an internal structure diagram of the microorganism separation and purification device of the present application.

Fig. 5 is a schematic structural diagram of the piston plate of the present application.

FIG. 6 is a schematic structural diagram of the mounting board of the present application.

Fig. 7 is a diagram of the use state of the mounting plate of the present application.

FIG. 8 is a schematic structural view of the support seat of the present application.

Fig. 9 is a schematic structural view of the tool body of the present application.

Fig. 10 is a schematic diagram of the installation of the coating head installed on the tool body of the present application.

Fig. 11 is a schematic diagram of the installation of dot matrix needles installed on the tool body of the present application.

In the picture:

1. Installation structure; 11. Installation plate; 12. Installation hole; 13. Limiting boss; 2. Connection structure; 21. Piston plate; 22. Jack; 3. Support structure; 31. Support seat; 32. Opening ;33, accommodating cavity; 34, limit groove; 4, separation and purification tool; 41, tool body; 411, external thread surface; 42, installation part; 43, operation part; 5, coating head; 6, dot matrix Needle.

Detailed ways

The following will clearly and completely describe the technical solutions in the embodiments of the application with reference to the drawings in the embodiments of the application. Apparently, the described embodiments are only some of the embodiments of the application, not all of them. Based on the embodiments in this application, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the scope of protection of this application.

As shown in Figure 1, the present application provides a microorganism separation and purification device, including: a connecting structure 2, which can be grasped by the mechanical arm of the automatic liquid transfer station; A separation and purification tool 4, the mechanical arm can drive multiple separation and purification tools 4 to perform separation and purification operations at the same time; the support structure 3, the installation structure 1 is supported on the support structure 3 when the connecting structure 2 is separated from the mechanical arm.

In the microorganism separation and purification device of the present application, a plurality of separation and purification tools 4 are installed on the installation structure 1, and the installation structure 1 is connected to the connection structure 2, and then the connection structure is grasped by the mechanical arm of the automatic liquid transfer station 2, so that multiple separation and purification tools 4 are driven by the mechanical arm to perform separation and purification operations at the same time, and before the connection structure 2 is grasped and after the separation and purification operation is completed, the installation structure 1 is supported by the support structure 3. Therefore, the present application The microbial separation and purification device can use the mechanical arm of the automatic pipetting station to realize the automation of microbial separation and purification operations, with high efficiency, and can avoid the problem of experimental errors caused by fatigue caused by repeated operations. In addition, it does not need to be equipped with other mechanical arms and control devices, and is low in cost and easy to implement.

In this embodiment, the robotic arm of the automatic liquid pipetting station grabs multiple pipetting tips to absorb a certain amount of bacterial liquid, and transfers the bacterial liquid to the medium plate to be separated and purified, thereby completing the transfer. liquid operation; then the mechanical arm of the automatic pipetting station first grabs the connection structure 2 to separate the installation structure 1 from the support structure, and then drives multiple separation and purification tools 4 to perform microbial separation and purification operations at the same time, so that the bacterial liquid is evenly distributed in the culture medium plate, thereby completing the microorganism separation and purification operation; finally, the mechanical arm places the installation structure 1 on the support structure 3, and releases the connection structure 2. It can be seen that the microorganism separation and purification device of the present application utilizes the mechanical arm of the automatic pipetting station to realize the automation of the microorganism separation and purification operation, which will not affect the pipetting operation of the mechanical arm, and can also separate the pipetting operation steps from the microorganisms. Better linkage between purification steps.

Optionally, the mechanical arm of the automatic liquid pipetting station first grasps the connection structure 2 to separate the installation structure 1 from the support structure, and first drives a plurality of separation and purification tools 4 to dip a certain amount of bacterial liquid or pick a single microbial cell at the same time , and then drive a plurality of separation and purification tools 4 to evenly distribute the dipped bacterial solution on the medium plate or transfer the picked single microbial cell to the medium plate, thereby completing the microorganism separation and purification operation; finally, the mechanical arm will The mounting structure 1 is placed on the supporting structure 3 and the connecting structure 2 is released.

As shown in Figure 1, Figure 2 and Figure 3, specifically, the mechanical arm of the automatic pipetting workstation has 96 channels, that is, it can simultaneously grab 96 pipetting tips for pipetting operation, therefore, the microbial separation of the present application In the purification device, 96 separation and purification tools 4 are installed on the

installation structure

1, 12 separation and purification tools 4 are arranged in a row at intervals along the length direction of the installation structure 1, and 8 rows of separation and purification tools 4 are spaced along the width direction of the installation structure 1 96 separation and purification tools 4 are simultaneously driven by the robotic arm of the automatic pipetting workstation to perform microbial separation and purification operations.

As shown in Fig. 1, Fig. 4 and Fig. 6, in the embodiment of the present application, installation structure 1 comprises installation plate 11, is arranged with a plurality of installation holes 12 at intervals on installation plate 11, and the two ends of separation and purification tool 4 are respectively The operating part 43 and the mounting part 42 , the separation and purification tool 4 are passed through the mounting hole 12 , the operating part 43 is placed below the mounting hole 12 , and the mounting part 42 is stuck above the mounting hole 12 . The structure is simple and the installation is convenient.

Specifically, the operation part 43 is the end where the separation and purification tool 4 is in contact with the microorganisms to perform the separation and purification operation of the microorganisms. The installation part 42 is the end where the separation and purification tool 4 is connected to the installation structure 1 . The separation and purification tool 4 includes a tool body 41 , an operating part 43 and a mounting part 42 . The diameter of the tool body 41 is slightly smaller than the diameter of the mounting hole 12 . The diameters of the installation part 42 and the operation part 43 are larger than the diameter of the installation hole 12 , the operation part 43 is detachably connected to the bottom end of the tool body 41 , and the installation part 42 is connected to the top end of the tool body 41 . Firstly, the bottom end of the tool body 41 is passed through above the installation hole 12 and placed below the installation hole 12 , and then the operation part 43 is installed on the bottom end of the tool body 41 .

Optionally, the separation and purification tool includes a tool body, an operating part and an installation part, and the diameter of the tool body is slightly smaller than the diameter of the installation hole. The diameters of the installation part and the operation part are larger than the diameter of the installation hole, the installation part is detachably connected with the top end of the tool body, and the operation part is connected with the bottom end of the tool body. Firstly, the top of the tool body is passed under the installation hole and placed above the installation hole, and then the installation part is installed on the top of the tool body, thereby completing the installation of the separation and purification tool. Optionally, the separation and purification tool has an integrated structure, and the diameter of the operating part is smaller than the diameter of the installation hole, and the diameter of the installation part is larger than the diameter of the installation hole. The operating part is passed through the installation hole from above the installation hole and placed under the installation hole, so as to complete the installation of the separation and purification tool.

As shown in FIG. 4 and FIG. 10 , in this embodiment, the operating part 43 of the separation and purification tool 4 is the coating head 5 . The bacterial solution is spread on the culture medium plate by the coating head 5 and uniformly dispersed. One end of the tool body 41 is screwed to the coating head 5 . By screwing the coating head 5 to the tool main body 41, the connection is firm and easy to replace.

As shown in FIG. 4 and FIG. 11 , in another embodiment, the operating part 43 of the separation and purification tool 4 is a dot matrix needle 6 . One end of the tool body 41 is threadedly connected with the dot matrix needle 6 . By threading the dot matrix needle 6 with the tool main body 41, the connection is firm and easy to replace. The robotic arm of the automatic liquid transfer station drives multiple dot array needles 6 to dip in the bacterial solution, and then drives the multiple dot matrix needles 6 to simultaneously dot the dipped bacterial liquid on the culture medium plate, thereby completing the microorganism separation and purification operation.

Specifically, as shown in FIG. 9 , the bottom end of the tool body 41 has an external threaded surface 411. According to needs (such as culture medium plates of different specifications), an appropriate operating part 43 is installed on the tool body 41 by threaded connection. Such as coating head 5 or dot matrix needle 6. Optionally, other detachable connection methods such as snaps and clamps may also be used between the operating part and the tool body.

As shown in FIG. 1 , FIG. 4 , FIG. 6 and FIG. 7 , in the embodiment of the present application, the separation and purification tool 4 can be slidably arranged along the installation hole 12 . When the separation and purification tool 4 is in contact with the culture medium plate for separation and purification operation, the separation and purification tool 4 can adaptively slide along the mounting hole 12 without being inserted into the culture medium plate. Specifically, there is a slidable space above the separation and purification tool 4 . The connection structure 2 is located above the installation structure 1 , and there is a certain gap between the installation part 42 of the separation and purification tool 4 and the connection structure 2 , so that the separation and purification tool 4 can slide along the installation hole 12 . Optionally, an elastic buffer structure is provided between the separation and purification tool and the connecting structure, so that the separation and purification tool can adaptively slide along the installation hole.

As shown in Fig. 4 and Fig. 5, in the embodiment of the present application, the mechanical arm of the automatic pipetting station is provided with a plurality of connecting columns, and the connecting structure 2 includes a piston plate 21, and the piston plate 21 is provided with a plurality of jacks 22, The insertion hole 22 has the same shape as the insertion opening of the pipette tip, and a plurality of connecting columns can be inserted into a plurality of insertion holes 22 . The mechanical arm of the automatic liquid pipetting station is plugged with the insertion openings of multiple pipetting tips through multiple connecting columns, so as to realize the grasping of multiple pipetting tips. In this application, the piston plate 21 is set, and the piston The plate 21 is provided with a plurality of sockets 22 having the same shape as the insertion opening of the pipetting tip, so that it can be used in conjunction with the mechanical arm of the automatic liquid pipetting station.

The piston plate 21 is detachably arranged above the installation structure 1 . By removing the piston plate 21 , it is convenient to assemble and disassemble a plurality of separation and purification tools 4 on the installation structure 1 . Specifically, the piston plate 21 is connected to the installation structure 1 through a connecting piece, buckle, clamp or other detachable connection methods.

As shown in Fig. 1, Fig. 4 and Fig. 8, in the embodiment of the present application, the support structure 3 includes a support seat 31, the support seat 31 has an accommodating cavity 33, the top of the support seat 31 has an opening 32, and the installation structure 1 is Supported on the top of the support base 31 , a plurality of separation and purification tools 4 extend from the opening 32 into the accommodating cavity 33 . The installation structure 1 is supported by the support base 31 , and the plurality of separation and purification tools 4 are extended into the accommodating cavity 33 , which can prevent the separation and purification tools 4 from contacting with other objects and being polluted before operation. Specifically, the supporting seat 31 generally has a frame structure. The size of the accommodating cavity 33 is not specifically limited, but the operating part 43 of the separation and purification tool 4 needs to be suspended in the accommodating cavity 33 , that is, it will not contact the table below and the supporting seat 31 .

As shown in FIG. 4 , FIG. 7 and FIG. 8 , the top of the support base 31 is provided with a limiting groove 34 , and the bottom of the installation structure 1 is provided with a limiting boss 13 , and the limiting boss 13 matches the limiting groove 34 . The installation structure 1 will not slide on the support base 31 along the length direction and the width direction of the support base 31 to cause the separation and purification tool 4 to deflect.

The above are only a few embodiments of the present application, and those skilled in the art can make various changes or modifications to the embodiments of the present application according to the contents disclosed in the application documents without departing from the spirit and scope of the present application.

Claims

A microorganism separation and purification device, including:

The connection structure can be grasped by the robotic arm of the automatic liquid transfer station;

The installation structure is connected with the connection structure, the installation structure is equipped with a plurality of separation and purification tools, and the mechanical arm can drive a plurality of the separation and purification tools to perform separation and purification operations at the same time;

A supporting structure, the mounting structure is supported on the supporting structure in a state where the connecting structure is separated from the robot arm.
The microorganism separation and purification device as claimed in claim 1, wherein,

The mounting structure includes a mounting plate, and a plurality of mounting holes are arranged at intervals on the mounting plate. The two ends of the separation and purification tool are respectively an operating part and a mounting part, and the separation and purification tool is passed through the mounting holes. Among them, the operation part is placed below the installation hole, and the installation part is clamped above the installation hole.
The microorganism separation and purification device as claimed in claim 2, wherein,

The separation and purification tool can be slidably arranged along the installation hole.
The microorganism separation and purification device as claimed in claim 1, wherein,

The mechanical arm of the automatic liquid pipetting station is provided with a plurality of connecting columns, and the connecting structure includes a piston plate, and the piston plate is provided with a plurality of jacks, and the jacks are connected to the insertion opening of the pipette tip. The shapes are the same, and multiple connecting posts can be plugged into multiple sockets.
The microorganism separation and purification device as claimed in claim 4, wherein,

The piston plate is detachably arranged above the mounting structure.
The microorganism separation and purification device as claimed in claim 1, wherein,

The support structure includes a support base, the support base has an accommodating cavity, the top of the support base has an opening, the installation structure is supported on the top of the support base, and a plurality of the separation and purification tools are separated from the The opening extends into the accommodating cavity.
The microorganism separation and purification device as claimed in claim 6, wherein,

The top of the support seat is provided with a limiting groove, and the bottom of the installation structure is provided with a limiting boss, and the limiting boss matches the limiting groove.
The microorganism separation and purification device as claimed in claim 2, wherein,

The separation and purification tool includes a tool body, one end of the tool body is threadedly connected to the operating part, and the other end of the tool body is connected to the installation part.
The microorganism separation and purification device as claimed in claim 8, wherein,

The operation part is a coating head.
The microorganism separation and purification device as claimed in claim 8, wherein,

The operating part is a dot matrix needle.