CN218969241U

CN218969241U - Nucleic acid extraction device

Info

Publication number: CN218969241U
Application number: CN202122078825.3U
Authority: CN
Inventors: 崔浩东; 戴恒; 茹晓玲; 叶东
Original assignee: Archean Gene Technology Shenzhen Co ltd
Current assignee: Archean Gene Technology Shenzhen Co ltd
Priority date: 2021-08-31
Filing date: 2021-08-31
Publication date: 2023-05-05
Anticipated expiration: 2031-08-31

Abstract

The utility model discloses a nucleic acid extraction device, which comprises a supporting frame and a plurality of magnetic frames/electromagnetic frames, wherein one side of each magnetic frame/electromagnetic frame is provided with a convex part, the other side of each magnetic frame/electromagnetic frame is provided with a concave part which can be in plug-in fit with the convex part, the supporting frame comprises two opposite end plates and a bottom plate connected with the two end plates, and the opposite positions of the two end plates are respectively provided with a fixed convex part and a fixed concave part which have the same shape as the convex part and the concave part on the magnetic frame. The utility model provides an electromagnetic force frame and a nucleic acid extraction device, which solve the problems that the existing magnetic bead preparation device is heavy and inflexible, and a plurality of magnetic force frames/electromagnetic force frames are not tightly connected and are easy to slip.

Description

Nucleic acid extraction device

Technical Field

The present utility model relates to a nucleic acid extraction device.

Background

The existing extraction method of free DNA mainly comprises a magnetic bead method, and the principle of the technology for extracting nucleic acid by the magnetic bead method is as follows: the superparamagnetism nano particles are adopted, and the principle that nucleic acid is adsorbed under the high-salt condition and low pH value and then is separated and purified under the low-salt and high pH value is utilized to separate and purify the sample nucleic acid, so that the high-flux automatic standardized operation can be realized due to the fact that the nucleic acid is separated and purified efficiently and simply only under the action of a magnetic field.

The magnetic bead method is classified into automatic extraction and manual extraction, has excellent matching property with an automatic machine, is more suitable for projects with larger sample size, and is most commonly used for automatic extraction, but requires special equipment, namely a nucleic acid extractor. When the sample size is small, the use automation can cause instrument abrasion and consumable waste, and the magnetic rack is more suitable for manual extraction; manual extraction still suffers from a number of disadvantages, such as: the magnetic force of the common magnetic rack is insufficient, and a small amount of magnetic beads are sunk at the bottom of the pipe and are not adsorbed; the centrifuge tube needs to be put into and out of the magnetic rack, so that time is consumed, and the probability of misoperation is increased; limited by the size and weight of the permanent magnets, high flux operation is difficult.

The conventional magnetic rack still has a plurality of defects although the conventional magnetic rack can meet the general experimental requirements of users. Comprising the following steps: the existing electromagnetic force frame is more than 10 holes, and cannot be flexibly adjusted according to experimental requirements; most magnetic force frames are of porous integral structures, occupy large space and are too heavy for experiments with small relative sample amounts.

The Chinese patent No. 201520662482.7 discloses an electromagnetic force frame for extracting nucleic acid by a magnetic bead method, which comprises a battery, a main switch, a ammeter, a slide rheostat and a plurality of groups of control branches formed by connecting electromagnets and independent switches in series, wherein the battery, the main switch, the ammeter and the plurality of groups of control branches which are connected in parallel are connected in series to form a main control loop. Each electromagnet is independently controlled through the parallel control branch, the operation is more flexible, the magnetic size of the electromagnet is adjusted by utilizing the sliding rheostat, and the problem of residual magnetic beads of the traditional magnetic frame is solved; meanwhile, the expansion of the module is realized by arranging an expansion interface, so that the processing capacity of the electromagnetic force frame is further increased. However, it has the following problems: each electromagnet is only used for extracting nucleic acid from one centrifuge tube, so that energy waste is caused; the magnetic field intensity generated by each electromagnet is the same, and different magnetic field intensities cannot be set for centrifugal tubes with single or multiple holes; there is no fixed structure between the extended electromagnetic force frames, which is easy to cause unstable electric connection and overturning of the electromagnetic force frames.

Chinese patent No. CN202022410778.3 discloses a magnetic rack and a nucleic acid extracting apparatus, the nucleic acid extracting apparatus comprising a set of electromagnetic racks and sample tubes placed in the tube holes. A magnetic stand, comprising: the test tube holder comprises a first wall plate, a second wall plate and a support frame fixedly connected between the first wall plate and the second wall plate, wherein test tube holes for inserting sample test tubes are formed in the first wall plate and/or the second wall plate, a first opening and a second opening are formed in the extending direction of the support frame, and magnetic blocks for gathering and adsorbing magnetic beads in the sample test tubes are arranged in the first opening and/or the second opening. The magnetic rack can adjust the magnetic attraction part and the magnetic attraction strength according to the experimental purposes, is small and exquisite in size, can be flexibly assembled into a plurality of nucleic acid extraction devices according to a reaction system, the number of samples and the like so as to adapt to corresponding experimental requirements, and is simple and portable. However, it has the following problems: the magnetic force is not adjustable, the magnetic attraction strength is adjusted by changing the magnet, and the operation is complex; the magnetic force frames are clamped and connected, and the magnetic force frames are easy to fall off when assembled together and move.

Disclosure of Invention

In view of the shortcomings of the prior art, the present utility model solves the above-mentioned problems by providing a nucleic acid extraction apparatus.

In order to achieve the above purpose, the technical scheme of the utility model is as follows:

the utility model provides a nucleic acid extraction device, includes the magnetic force frame, magnetic force frame one side is equipped with the convex part, and the opposite side is equipped with can with convex part grafting complex concave part, its characterized in that still includes the supporting rack, the supporting rack includes two relative end plates and connects the bottom plate of two end plates, two end plates relative position is equipped with fixed convex part and the fixed concave part the same with magnetic force frame upper lobe part and concave part shape respectively.

Preferably, the magnetic frame comprises a first wall plate, a second wall plate and a support frame fixedly connected between the first wall plate and the second wall plate, test tube holes are formed in the first wall plate and/or the second wall plate, the convex part and the concave part are respectively arranged on two sides of the support frame, a plurality of through holes are formed in the extending direction of the support frame, and magnets are arranged in the through holes.

Preferably, the magnetic force frame is an electromagnetic force frame, including: the test tube comprises a first wall plate, a second wall plate and a support frame fixedly connected between the first wall plate and the second wall plate, wherein test tube holes are formed in the first wall plate and/or the second wall plate, the convex part and the concave part are respectively arranged on two sides of the support frame, a plurality of through holes are formed in the extending direction of the support frame, and electromagnets are arranged in the through holes.

Preferably, a switch and a potentiometer/rheostat connected in series with the electromagnet are arranged on the first wall plate.

Preferably, an indicator light connected in series with the electromagnet is arranged on the first wall plate. The electromagnetic force frame is used for indicating the working state of the electromagnetic force frame, and is convenient to overhaul and replace.

Preferably, the convex portion is a T-shaped plate, and the concave portion is a T-shaped groove.

Preferably, the convex portion and the concave portion are provided with electrical connection units at corresponding positions.

Preferably, the electrical connection unit is a conductive contact arranged on the convex part and a conductive sheet arranged at a position corresponding to the concave part. The conductive contact is connected with a spring piece or a spring, and is arranged on the convex part in a telescopic manner, a groove is arranged on the concave part, and the conductive piece is arranged in the groove. The conductive contact is clamped into the groove to prevent sliding and falling between the electromagnetic force frames.

Preferably, one end plate is provided with a power supply and/or an electrical interface; the power supply is electrically connected with the electromagnetic force frame through the electric connection unit, and the electric interface is connected with an external power supply.

Preferably, the electromagnetic force rack is further provided with a power adapter for converting an external alternating current power supply into direct current power, and supplying power to the electromagnetic force rack and/or charging the power supply through an electric interface. The continuous use and the mobility of the nucleic acid extraction device can be considered.

Preferably, the electromagnetic force device comprises a plurality of electromagnetic force frames, wherein adjacent electromagnetic force frames are connected through plug-in fit between the convex part and the concave part; the electromagnetic irons of the electromagnetic force frame are connected in parallel.

Preferably, the end plate is provided with a power supply and a main switch; an end plate is provided with a mounting concave corresponding to the convex part of the electromagnetic force frame, the mounting concave is provided with a conductive sheet, and the power supply is electrically connected with the conductive contact of the electromagnetic force frame through the conductive sheet.

Preferably, the end plate is provided with a plurality of groups of fixing convex parts and fixing concave parts.

The utility model discloses a nucleic acid extraction device, which supports a magnetic rack through a support plate, better fixes the magnetic rack and simultaneously prevents a plurality of connected magnetic racks from sliding off. Furthermore, when the electromagnetic force frames are used, the adjacent electromagnetic force frames and the end plates are connected through the insertion fit between the convex parts and the concave parts, and the conductive contacts are clamped in the grooves to prevent the electromagnetic force frames from sliding in the vertical direction.

The utility model has the following beneficial effects:

the utility model provides a nucleic acid extraction device, which solves the problems that the existing magnetic bead extraction device is heavy and inflexible, and a plurality of magnetic force frames and electromagnetic force frames are not tightly connected. Meanwhile, the problem that the magnetic field intensity of the existing magnetic bead preparation device is not adjustable is solved.

Drawings

FIG. 1 is a schematic diagram of an embodiment 1 of a nucleic acid isolation apparatus of the present disclosure;

fig. 2 is a schematic front view of an electromagnetic force frame of embodiment 1 of the present disclosure;

FIG. 3 is a schematic view of the electromagnetic force frame of embodiment 1 of the present disclosure;

FIG. 4 is a circuit diagram of an embodiment 1 of the nucleic acid isolation apparatus of the present disclosure;

FIG. 5 is a schematic diagram showing an example 2 of a nucleic acid isolation apparatus of the present disclosure;

fig. 6 is a schematic view of a magnetic stand according to embodiment 2 of the present disclosure.

Detailed Description

The utility model is further illustrated by the following examples.

Example 1

As shown in fig. 1 to 4, the nucleic acid extracting apparatus includes a plurality of electromagnetic force frames and a support frame 100. The electromagnetic force frame comprises a first wall plate 1, a second wall plate 2 and a support frame 3 fixedly connected between the first wall plate 1 and the second wall plate 2, wherein test tube holes 4 are formed in the first wall plate 1 and the second wall plate 2, two through holes 5 are formed in the extending direction of the support frame 3, and electromagnets 6 are arranged in the through holes 5. A convex part 8 is arranged on one side of the support frame 3, a concave part 9 which can be in plug-in fit with the convex part is arranged on the other side of the support frame, and a potentiometer 10 and a switch 11 are arranged on the first wall plate 1; two elastically telescopic conductive contacts 7 are arranged on the convex part 8, two grooves are arranged on the concave part 9 corresponding to the conductive contacts 7, and conductive sheets 12 are arranged in the two grooves. The circuit diagram of the electromagnetic force frame is shown in fig. 3, the two corresponding conductive contacts 7 and the two conductive sheets 12 are respectively and electrically connected through two wires, and the switch 11, the potentiometer 10 and the electromagnet 6 are connected in series and then are bridged between the two wires. The switch 11, potentiometer 10 and electromagnet 6 are preferably also connected in series with an indicator light (not shown). The support frame 100 includes an end plate 101, an end plate 102, and a bottom plate 103, the end plate 101 is provided with a mounting concave portion that mates with the convex portion 8, and the end plate 102 is provided with a mounting convex portion that mates with the concave portion 9. The support frame further includes a switch provided on the end plate 101, a power source (not shown) provided in the end plate 101 and a conductive sheet provided in the mounting recess, the power source supplying power to the electromagnetic force frame through the conductive sheet.

Example 2

As shown in FIGS. 5 and 6, the nucleic acid isolation apparatus includes a plurality of magnetic racks and a support 100'. The electromagnetic force frame comprises a first wall plate 1', a second wall plate 2' and a support frame 3 'fixedly connected between the first wall plate 1' and the second wall plate 2', wherein test tube holes 4' are formed in the first wall plate 1 'and the second wall plate 2', two through holes 5 'are formed in the extending direction of the support frame 3', and magnets 6 'are arranged in the through holes 5'. One side of the supporting frame 3' is provided with a convex part 8', and the other side of the supporting frame is provided with a concave part 9' which can be in plug-in fit with the convex part. The support 100 'comprises an end plate 101', an end plate 102 'and a bottom plate 103', wherein the end plate 101 'is provided with a mounting concave part matched with the convex part 8', and the end plate 102 'is provided with a mounting convex part matched with the concave part 9'.

Example 3

Unlike embodiment 1, the end plate 101 of the support frame 100 is further provided with an electrical interface, and the nucleic acid isolation apparatus further includes a power adapter that converts an external ac power into a dc power, supplies power to the electromagnetic force frame through the electrical interface, and simultaneously charges the power.

The foregoing is merely a preferred embodiment of the present utility model and it should be noted that modifications and adaptations to those skilled in the art may be made without departing from the principles of the present utility model, which are intended to be comprehended within the scope of the present utility model.

Claims

1. The utility model provides a nucleic acid extraction device, includes the magnetic force frame, magnetic force frame one side is equipped with the convex part, and the opposite side is equipped with can with convex part grafting complex concave part, its characterized in that still includes the supporting rack, the supporting rack includes two relative end plates and connects the bottom plate of two end plates, two end plates relative position is equipped with fixed convex part and the fixed concave part the same with magnetic force frame upper lobe part and concave part shape respectively.

2. The nucleic acid isolation apparatus according to claim 1, wherein the magnetic rack comprises a first wall plate, a second wall plate, and a support frame fixedly connected between the first wall plate and the second wall plate, wherein the first wall plate and/or the second wall plate is provided with test tube holes, the protruding portions and the recessed portions are respectively arranged on two sides of the support frame, a plurality of through holes are formed along the extending direction of the support frame, and magnets are arranged in the through holes.

3. The nucleic acid extraction apparatus of claim 1, wherein the magnetic rack is an electromagnetic rack comprising: the test tube comprises a first wall plate, a second wall plate and a support frame fixedly connected between the first wall plate and the second wall plate, wherein test tube holes are formed in the first wall plate and/or the second wall plate, the convex part and the concave part are respectively arranged on two sides of the support frame, a plurality of through holes are formed in the extending direction of the support frame, and electromagnets are arranged in the through holes.

4. The nucleic acid isolation apparatus according to claim 3, wherein a switch and a potentiometer/varistor are provided in series with the electromagnet on the first wall plate.

5. The nucleic acid isolation apparatus according to claim 3, wherein an indicator lamp connected in series with the electromagnet is provided on the first wall plate.

6. The nucleic acid isolation apparatus according to claim 2 or 3, wherein the convex portion is a T-shaped plate and the concave portion is a T-shaped groove.

7. The nucleic acid isolation apparatus according to claim 3, wherein the protruding portion and the recessed portion are provided with electrical connection means at positions corresponding to each other.

8. The nucleic acid isolation apparatus according to claim 3, wherein one end plate is provided with a power source and/or an electrical interface; the power supply is electrically connected with the electromagnetic force frame through the electric connection unit, and the electric interface is connected with an external power supply.

9. The nucleic acid isolation apparatus of claim 8, further comprising a power adapter that converts an external ac power source to dc power, and provides power to the electromagnetic force rack and charges the power source via the electrical interface.

10. The nucleic acid extraction apparatus as claimed in claim 8, comprising a plurality of electromagnetic force frames, adjacent ones of the electromagnetic force frames being connected by a plug-in fit between the convex portion and the concave portion; the electromagnetic irons of the electromagnetic force frame are connected in parallel.