CN215856147U - Electromagnetic force frame and nucleic acid extraction element - Google Patents

Abstract

The utility model discloses an electromagnetic force frame and a nucleic acid extraction device. The nucleic acid extraction device comprises an end plate and a plurality of electromagnetic force frames, wherein the end plate, the electromagnetic force frames and the electromagnetic force frames are mutually inserted. The problem of current electromagnetism frame can't adjust magnetic field intensity in a flexible way, connect untight between the many magnetism frame is solved.

Description

Electromagnetic force frame and nucleic acid extraction element

Technical Field

The utility model relates to an electromagnetic force frame and a nucleic acid extraction device.

Background

The existing extraction method of free DNA mainly comprises a magnetic bead method, and the principle of the magnetic bead method for extracting nucleic acid is as follows: the superparamagnetic nanoparticle is adopted, nucleic acid is adsorbed under the condition of high salt and low pH, and then the separation and purification of the sample nucleic acid are carried out by utilizing the principle of separating the nucleic acid under the condition of low salt and high pH, and the nucleic acid is separated and purified efficiently and simply under the action of a magnetic field, so that the high-throughput automatic standardized operation can be realized.

The magnetic bead method is divided into automatic extraction and manual extraction, the magnetic bead method is extremely well matched with an automatic machine, is more suitable for projects with large sample sizes, is most commonly used for automatic extraction, and needs special equipment, namely a nucleic acid extractor. When the sample size is small, the use of automation can cause the abrasion of instruments and the waste of consumables, and the magnetic rack is more suitable for manual extraction; manual extraction still faces many deficiencies, such as: the common magnetic rack has insufficient magnetic force, and a small amount of magnetic beads sink to the bottom of the tube and are not adsorbed; the centrifugal tube needs to be put in and put out of the magnetic frame, so that time is consumed, and the probability of misoperation is increased; limited by the size and weight of the permanent magnets, high flux operation is difficult to achieve.

Although the existing conventional magnetic rack can meet the general experimental requirements of users, the existing conventional magnetic rack still has many defects. The method comprises the following steps: the existing magnetic frame is more than 10 holes, and can not be flexibly adjusted according to the experiment requirement; most magnetic force frames are of porous integral structures, occupy large space and are too heavy for experiments with small relative sample amount; as most of the magnetic racks on the market are produced to adapt to the diameter difference of micro test tube manufacturers, the maximum diameter of the existing micro test tube is forced to be selected as a template, so that part of the micro test tubes cannot be firmly attached to the magnetic racks, and the falling risk exists in the experiment needing to be reversed and mixed uniformly; most magnetic force frame inhale the position singleness and inhale the scope partially down in magnetism, when handling the experiment that single sample size is more, have the uneven phenomenon of elution, cause the sample extravagant.

Chinese utility model patent CN201520662482.7 discloses an electromagnetic force frame for extracting nucleic acid by the magnetic bead method, including battery, master switch, galvanometer, slide rheostat, a plurality of groups control branch that comprises electro-magnet and independent switch series connection, battery, master switch, galvanometer and a plurality of groups of parallelly connected control branch establish ties and constitute total control return circuit. The independent control of each electromagnet is realized through the parallel control branch, the operation is more flexible, the magnetic size of the electromagnet is adjusted by utilizing the slide rheostat, and the problem that magnetic beads are remained in the traditional magnetic rack is solved; meanwhile, the expansion of the module is realized by arranging the expansion interface, and the processing capacity of the electromagnetic force frame is further increased. However, it has the following problems: each electromagnet is only used for extracting nucleic acid of one centrifugal tube, so that energy waste is caused; the magnetic field intensity generated by each electromagnet is the same, and different magnetic field intensities can not be set for a single centrifugal tube or centrifugal tubes with a plurality of hole sites; there is no fixed structure between the extended electromagnetic force frames, which easily causes unstable electric connection and overturn of the electromagnetic force frames.

Chinese utility model patent CN202022410778.3 discloses a magnetic rack and nucleic acid extraction device, including a set of magnetic rack and place in the sample test tube in the test tube hole. A magnetic frame comprising: first wallboard, second wallboard and rigid coupling in first wallboard with support frame between the second wallboard, first wallboard and/or the test tube hole that is used for inserting the sample test tube is offered to the second wallboard, follows the support frame extending direction is equipped with first trompil and second trompil, install in first trompil and/or the second trompil will the absorptive magnetic path of magnetic bead gathering in the sample test tube. The magnetic force frame can be adjusted according to the experiment purpose and inhale the position and inhale intensity magnetically, and is small and exquisite, can assemble into multiple nucleic acid extraction element with the corresponding experiment demand of adaptation in a flexible way according to reaction system and sample quantity etc. simply portably. However, it has the following problems: the magnetic force is not adjustable, the adjustment of the magnetic attraction strength needs to be realized by replacing the magnet, and the operation is complex; the magnetic force frames are clamped and connected with each other, and are easy to fall off when moving.

SUMMERY OF THE UTILITY MODEL

In order to overcome the defects of the prior art, the utility model provides an electromagnetic rack and a nucleic acid extraction device, so as to solve the technical problems.

In order to achieve the purpose, the technical scheme of the utility model is as follows:

an electromagnetic rack comprising: first wallboard, second wallboard and rigid coupling in first wallboard with support frame between the second wallboard, the test tube hole has been seted up to first wallboard and/or the second wallboard, its characterized in that follows the support frame extending direction is equipped with a plurality of through-holes, be equipped with the electro-magnet in the through-hole.

Preferably, a potentiometer or a rheostat connected with the electromagnet in series is arranged on the first panel.

Preferably, an indicator light connected with the electromagnet in series is arranged on the first panel.

Preferably, one side of the support frame is provided with a convex part, and the other side of the support frame is provided with a concave part which can be in inserted connection with the convex part.

Preferably, the convex part is a T-shaped plate extending along one side of the support frame, and the concave part is a T-shaped groove extending along the other side of the support frame.

Preferably, an electrical connection unit is disposed at a position corresponding to the convex portion and the concave portion.

Preferably, the electrical connection unit is a conductive contact provided on the convex portion and a conductive sheet provided at a position corresponding to the concave portion. The conductive contact is connected with a spring piece or a spring, and is arranged on the convex part in a telescopic manner, the concave part is provided with a groove, and the conductive piece is arranged in the groove.

The utility model also discloses a nucleic acid extraction device which comprises a group of electromagnetic force racks and sample test tubes placed in the test tube holes. Preferably, a power supply and a main switch are also included.

The utility model also discloses a nucleic acid extraction device, which comprises an end plate and a plurality of electromagnetic force frames, wherein the end plate is also provided with a concave part matched with the convex part; adjacent between the electromagnetism frame, between electromagnetism frame and the end plate through the convex part with the grafting cooperation between the concave part is connected, and conductive contact is by the joint in the recess, prevents between the electromagnetism frame at vertical direction slip. The end plate also comprises a power supply and a main switch, the power supply and the main switch are connected in series and then connected with the two conducting strips of the end plate, and the electromagnets between the electromagnetic force frames are connected in parallel.

The utility model has the following beneficial effects:

the utility model provides an electromagnetic force frame and a nucleic acid extraction device, and solves the problems that the magnetic field intensity of the existing magnetic bead preparation device is not adjustable, and a plurality of magnetic force frames are not tightly connected.

Drawings

FIG. 1 is a schematic diagram of an embodiment of an electromagnetic stand according to the present disclosure;

FIG. 2 is a schematic rear view of an embodiment of an electromagnetic stand according to the present disclosure;

FIG. 3 is a circuit diagram of an embodiment of the electromagnetic stand disclosed herein;

FIG. 4 is a schematic view of an embodiment of the nucleic acid isolation apparatus according to the present disclosure;

FIG. 5 is a schematic view of an end plate of the nucleic acid isolation apparatus according to the present disclosure;

FIG. 6 is a schematic diagram showing a circuit of the nucleic acid isolation apparatus according to the present invention.

Detailed Description

The utility model is further illustrated by the following examples.

Example 1

As shown in the attached drawing 1, the electromagnetic force frame comprises a first wall plate 1, a second wall plate 2 and a support frame 3 fixedly connected between the first wall plate 1 and the second wall plate 2, wherein a test tube hole 4 is formed in the first wall plate 1 and the second wall plate 2, two through holes 5 are formed in the support frame 3 in the extending direction, and an electromagnet 6 is arranged in each through hole 5. A convex part 8 is arranged on one side of the support frame 3, a concave part 9 capable of being in plug-in fit with the convex part is arranged on the other side of the support frame, and a potentiometer 10 and a switch 11 are arranged on the first wall plate 1; the convex part 8 is provided with two elastically stretchable conductive contacts 7, and as shown in fig. 2, two grooves are provided on the concave part 9 corresponding to the conductive contacts 7, and conductive sheets 12 are provided in the two grooves. The circuit diagram of the electromagnetic force frame is shown in fig. 3, the two corresponding conductive contacts 7 and the two conductive sheets 12 are electrically connected through two wires respectively, and the switch 11, the potentiometer 10 and the electromagnet 6 are connected in series and then bridged between the two wires. Preferably, the switch 11, the potentiometer 10 and the electromagnet 6 are also connected in series with an indicator light (not shown).

Example 2

As shown in FIGS. 4 and 5, the nucleic acid extracting apparatus comprises an end plate and a plurality of electromagnetic force frames connected to the end plate, wherein adjacent electromagnetic force frames are connected by the insertion fit between the convex part and the concave part; the end plate is provided with a power supply (not shown) and a main switch; the end plate is provided with a concave part 9 'corresponding to the convex part 8 of the electromagnetic force frame, the concave part 9' is provided with two conducting strips corresponding to the conducting contact 7, one pole of the power supply is connected with one conducting strip, and the other pole of the power supply is connected with the other conducting strip after being connected with the main switch. As shown in FIG. 6, the circuit diagram of the nucleic acid isolation apparatus is that a switch 11, a potentiometer 10 and an electromagnet 6 of a single electromagnetic rack are connected in series, and the circuits of the electromagnetic racks are connected in parallel.

The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various modifications and decorations can be made without departing from the principle of the present invention, and these modifications and decorations should also be regarded as the protection scope of the present invention.

Claims (8)

1. An electromagnetic rack comprising: the test tube comprises a first wall plate, a second wall plate and a support frame fixedly connected between the first wall plate and the second wall plate, wherein the first wall plate and/or the second wall plate are/is provided with test tube holes; a potentiometer or a rheostat connected with the electromagnet in series is arranged on the first wall plate; one side of the support frame is provided with a convex part, and the other side of the support frame is provided with a concave part which can be matched with the convex part in an inserting way.

2. The electromagnetic rack of claim 1, wherein the first wall plate is provided with an indicator light connected in series with the electromagnet.

3. The electromagnetic rack of claim 1, wherein the protrusion is a T-shaped plate extending along one side of the support frame and the recess is a T-shaped slot extending along the other side of the support frame.

4. The electromagnetic rack according to claim 1 or 3, wherein the convex portion and the concave portion are provided with an electrical connection unit at corresponding positions.

5. The electromagnetic rack according to claim 4, wherein the electrical connection unit is a conductive contact provided on the convex portion and a conductive sheet provided at a position corresponding to the concave portion.

6. A nucleic acid isolation apparatus comprising a plurality of the electromagnetic racks according to any one of claims 1 to 5.

7. A nucleic acid isolation apparatus comprising at least two sets of the electromagnetic force racks according to any one of claims 4 to 5, wherein adjacent electromagnetic force racks are connected by a mating fit between the convex portion and the concave portion; the electromagnets of the electromagnetic force frame are connected in parallel.

8. The nucleic acid extraction apparatus according to any one of claims 6 to 7, further comprising an end plate provided with a power supply and a main switch; the end plate is provided with a concave part corresponding to the convex part of the electromagnetic force frame, and the power supply is electrically connected with the electromagnetic force frame through a conducting strip.

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