Tissue engineering skin model culture support
Technical Field
The utility model belongs to the technical field of biomedical tissue engineering, and particularly relates to a tissue engineering skin model culture bracket.
Background
The tissue engineering skin model as a substitute for animal experiments is widely applied to the safety and efficacy detection and evaluation of products such as cosmetics, medicines and the like. The tissue engineering skin model is prepared through culturing seed cell in vitro in liquid and gas-liquid medium to realize the proliferation and differentiation of skin cell in vitro and form complete skin structure. The skin model belongs to a sterile product, and the whole operation process needs to ensure sterile operation. At present, most of skin models are constructed in cell culture chambers, common cell culture chambers are divided into a suspension type cell culture chamber and a standing type cell culture chamber, the basic shape of the suspension type cell culture chamber is a cup-shaped container, a bracket is designed at the cup mouth, the chamber can be suspended on a culture pore plate, and the cell culture chamber is usually required to be matched with a standard cell culture plate with a corresponding specification for use; the standing cell culture cell is also mostly a container filled in a cup, and the difference is that support legs are distributed at the bottom of the cup, so that the cell can stand and the bottom surface is suspended, and the cell culture cell is placed in a single-hole plate or a culture dish to be cultured in a standing mode during use. The two model culture methods have the following defects: firstly, the operation is not beneficial to large-scale production operation in terms of convenience degree of operation and quality control; secondly, the supporting angle of the small culture chamber is low, and the gas-liquid surface culture condition of the skin model in the construction process is difficult to control.
To the above problem, a skin model's culture apparatus has been designed in patent CN201420500545.4, including the box body, the upper cover, cultivate extension board and cultivate the cell, there are the liquid changing district and the cultivation district of intercommunication in the box body, be equipped with equal altitude stand in the cultivation district, be used for supporting the cultivation extension board, it is downthehole at the round of cultivation extension board to cultivate the cell embedding, hang in cultivation district top, the upper cover has the draw-in groove with box body bottom support complex, make the box body can fix and stack, the device can cultivate 48 skin models at most 1 time, guarantee the required gas-liquid face culture condition of skin model construction in-process through box body edge stand. The patent CN201720706095.8 discloses a skin model culture device, which comprises a device box, a device upper cover arranged on the device box, a culture support plate and a plurality of culture cells; the device is covered and is provided with liquid feeding hole and liquid feeding handhole door, branch row handle hole and branch row handle handhole door to and supplementary handle, add the culture solution for the device box body through the liquid feeding hole, operate the cell through dividing row handle hole, supplementary handle is used for opening the device upper cover.
The skin model culture devices mentioned in the above documents are all directed to a diameter of 10mm or less and a growth area of less than 1.0cm2The cell culture chamber of (1) does not have a culture device aiming at a large-size cell culture chamber with the diameter exceeding 20mm at present, along with the development of in vitro substitution detection technology, a small-area skin model can not meet the increasingly diversified application requirements of researchers, and a plurality of detection instrument equipment cannot be used due to the restriction of the model area, so that skin models with different sizes and specifications need to be developed. The method is characterized in that a large-size skin model is constructed, the number of inoculated cells is increased, the amount of culture liquid required by growth of the skin model is correspondingly increased, the depth of a standard cell culture pore plate is limited, the optimal liquid addition volume is 1-2ml, the nutritional requirement in the growth process of the model cannot be met far away, if the volume of the culture liquid is increased, a cell culture small chamber can float, the culture liquid is easy to overflow, and the pollution risk is increased. The Corning company has developed the cell culture board of deepening type for this reason specially, but the price is expensive, and for disposable, cultivates the in-process moreover, need operate one by one to the culture cell and trade the liquid, causes the difference of pollution and culture condition easily for the quality between the skin model of same batch is different, is unfavorable for large-scale production and uses.
SUMMERY OF THE UTILITY MODEL
The utility model aims to solve the problems in the prior art, provides a tissue engineering skin model culture bracket which is low in cost, reusable and convenient to operate, can ensure the quality stability and uniformity among skin models, and is suitable for large-scale production of large-scale skin models.
In order to achieve the purpose, the utility model adopts the following technical scheme:
providing a tissue engineering skin model culture bracket, wherein the tissue engineering skin model culture bracket comprises a panel and a strut connected to the bottom of the panel; at least one bracket unit is arranged on the panel. The support column is used for supporting the panel, and the support unit is used for supporting the tissue engineering skin model culture chamber.
Preferably, the panels are approximately hexagonal.
Preferably, the support unit comprises 6 peripheral support units and 1 central through hole support unit, and the peripheral support units are arranged in a regular hexagon.
Preferably, the peripheral support unit is connected with the central support unit through a connecting rod, and the length of the connecting rod is 3 mm.
Preferably, a connecting part is arranged between the peripheral bracket units, and the shape of the connecting part is approximately trapezoidal. The connecting part is used for resisting deformation of the culture bracket in an autoclave sterilization process.
Preferably, each lower part of the approximate trapezoid is connected with a pillar with the same height, and the pillar is cylindrical.
Preferably, the height of the pillars is 17mm and the diameter is 2 mm. After the tissue engineering skin model culture chamber is placed in the bracket unit, the bottom of the tissue engineering skin model culture chamber is 2-8mm away from the bottom surface of the support column so as to provide nutrition supply for the skin model in the culture process.
Preferably, the shape of the support unit is circular ring, and the circle center distance of the support unit is 40-48 mm. For example 40mm, 42mm, 44mm, 45mm, 46mm, 47mm or 48 mm. The structural design of the scaffold unit and the struts may be used to reduce the accumulation of culture fluid between the struts and the culture chamber.
Preferably, the thickness of the panel is 1.8-3 mm. For example, 1.8mm, 2.0mm, 2.2mm, 2.5mm, 2.8mm or 3.0mm, to resist deformation of the culture stent during use, to extend the stent life, and to stabilize the skin model quality.
In another aspect, the utility model provides the application of the tissue engineering skin model culture scaffold in culturing a 3D full-thickness skin model.
Drawings
FIG. 1 is a schematic structural diagram of a tissue engineering skin model culture scaffold provided in an embodiment of the present invention;
FIG. 2 is a schematic structural diagram of a tissue engineering skin model culture scaffold according to a preferred embodiment of the present invention;
FIG. 3 is a side view of a tissue engineering skin model culture scaffold according to a preferred embodiment of the present invention.
Reference numerals: 10-panel, 20-pillar, 11-brace unit, 111-peripheral brace unit, 112-central brace unit, 12-connecting rod, 13-connecting portion.
Detailed Description
The technical solutions in the embodiments of the present invention will be described in detail below with reference to the accompanying drawings in the embodiments of the present invention.
Examples
Referring to fig. 1 to 3, the present embodiment provides a tissue engineering skin model culture scaffold, which includes a panel 10 and a pillar 20 connected to the bottom of the panel; at least one rack unit 11 is provided on the panel 10. The support column 20 is used for supporting the panel 10, and the support unit 11 is used for supporting the tissue engineering skin model culture chamber. The tissue engineering skin model culture chamber is a common culture chamber sold in the market.
In some embodiments, the panel 10 is approximately hexagonal.
In some embodiments, the number of stent units 11 is 7, comprising 6 peripheral stent units and 1 central through-hole stent unit, the peripheral stent units being in a regular hexagonal arrangement.
In some embodiments, the peripheral frame units and the central frame unit are connected by a connecting rod 12, the connecting rod 12 having a length of 3 mm.
In some embodiments, a connecting portion 13 is provided between the peripheral stand units, and the shape of the connecting portion 13 is approximately trapezoidal. The connection portion 13 is used to resist deformation during autoclaving of the culture stent.
In some embodiments, a post 20 of equal height is attached to each lower portion of the proximal trapezoid, the post 20 being cylindrical.
In some embodiments, the struts 20 are 17mm in height and 2mm in diameter. After the tissue engineering skin model culture chamber is placed on the bracket unit 11, the bottom of the tissue engineering skin model culture chamber is 2-8mm away from the bottom surface of the support column, so that the aggregation of culture solution formed by the bottom of the culture chamber and the bottom surface of a culture dish due to the action of surface tension is eliminated, and the nutrition supply of the skin model in the culture process is provided.
In some embodiments, the shape of the rack unit 11 is circular ring, and the circle center distance of the rack unit 11 is 40-48 mm. For example 40mm, 42mm, 44mm, 45mm, 46mm, 47mm or 48 mm. The structural design of the scaffold unit 11 and the struts 20 can be used to reduce the accumulation of culture fluid between the struts and the culture chamber.
In some embodiments, the thickness of the panel 10 is 1.8-3 mm. For example, 1.8mm, 2.0mm, 2.2mm, 2.5mm, 2.8mm or 3.0mm, to resist deformation of the culture stent during use, to extend the stent life, and to stabilize the skin model quality.
In some embodiments, the utility model provides the application of the tissue engineering skin model culture scaffold in culturing a 3D full-thickness skin model.
The use method of the tissue engineering skin model culture bracket of the utility model comprises the steps of placing the culture bracket which is sterilized by high pressure into most commercial disposable culture dishes with standard specification of 150mm on the market, and then placing culture cells into bracket units one by one; the skin model culture bracket is suitable for large-scale skin model construction, is convenient to operate, can be repeatedly used under high pressure, greatly reduces the using amount of culture solution, reduces the culture space, reduces the production cost, can reduce the pollution risk, ensures the smoothness of products and the stability of quality, and is suitable for large-scale production of large-scale tissue engineering skin.
The above description is only an embodiment of the present application, but the scope of the present application is not limited thereto, and any changes or substitutions within the technical scope of the present disclosure should be covered by the scope of the present application. Therefore, the protection scope of the present application shall be subject to the protection scope of the claims.