CN207259519U - A kind of organization engineering skin model culture apparatus - Google Patents
A kind of organization engineering skin model culture apparatus Download PDFInfo
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- CN207259519U CN207259519U CN201720706095.8U CN201720706095U CN207259519U CN 207259519 U CN207259519 U CN 207259519U CN 201720706095 U CN201720706095 U CN 201720706095U CN 207259519 U CN207259519 U CN 207259519U
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Abstract
The utility model embodiment provides a kind of organization engineering skin model culture apparatus; it is related to biomedical tissue field of engineering technology; the organization engineering skin model culture apparatus is easy to operation and can reduce pollution risk; it can ensure product quality at the same time, and be suitable for large-scale production organization engineering skin.Organization engineering skin model culture apparatus includes device box body, the device upper cover being arranged on device box body, and culture support plate and multiple culture cells；Device box body is used to accommodate culture cell and culture support plate；Stent is provided with the bottom of device box body, stent is used to support device box body；Multiple contour pillars are provided with the inside of device box body, pillar is used to support culture support plate；On device liquid filling hole and liquid feeding port lid are provided with lid, divide row handle hole and a point row operation port lid, and auxiliary handle, be that device box body adds nutrient solution by liquid filling hole, divide row handle hole corresponding culture cell by a point row operation hole operation, auxiliary handle is used for opening device upper cover.
Description
Technical field
It the utility model is related to biomedical tissue field of engineering technology, more particularly to a kind of training of organization engineering skin model
Support device.
Background technology
Organization engineering skin model is widely used to the peace of the products such as cosmetics, medicine as the substitute of zoopery
Full property and both effectiveness detection, evaluation.Skin model belongs to sterile product, and whole operation process is both needed to ensure sterile working.At present
Such skin model is more to be built in cell is cultivated, and the diameter of common culture cell is mostly the tubular container of 0.9cm, and base is divided
Branch angle is furnished with, cell standing can be made and bottom surface is hanging.Culture cell is individually placed in cell culture plate during use and carries out structure
Build culture.This building mode has some following deficiency：When either from the convenient degree of operation, or in quality control all
It is unfavorable for large-scale production operation；Second, the branch angle of culture cell is relatively low, gas-liquid face culture of the skin model in building process
The bad control of condition, and this process is the key point of skin model structure.It is a kind of convenient to need to design for these reasons, easily
In the skin model process units of large-scale production.
Devised in the patent of application number 201420500545.4 a kind of small containing box body, upper cover, culture support plate and culture
The skin model culture apparatus of room, the device can at most cultivate 48 skin models 1 time, pass through box body edge column and ensure skin
Gas-liquid face condition of culture needed for model construction process, but this device still suffers from problems with：First, being integrated of upper cover is set
Meter, skin model cultivation stage under liquid need to open upper cover repeatedly and be operated, all models will exposure for a long time with environment,
In the presence of very big pollution risk；2nd, upper cover lacks handle, and the culture apparatus coverage in 48 holes is about 30cm2, and one hand is difficult to open
Open, make troubles to operation；3rd, middle culture support plate is only supported by several columns of box body lower edge, cultivates the smooth of support plate
Degree is difficult to maintain, and when especially carrying out structure culture in addition culture cell, multiple cells are easy to plus the weight of content
Cause middle support plate to sink to deforming, be unable to reach gas-liquid face condition of culture, cause structure culture failure.Therefore, with skin mould
The increasingly increase of type dosage, design it is a kind of it is more convenient, more stable model process units is significant.
The content of the invention
The utility model embodiment provides a kind of organization engineering skin model culture apparatus, the organization engineering skin model
Culture apparatus is easy to operation and can reduce pollution risk, while can ensure product quality, and is suitable for large-scale production tissue work
Journey skin.
To reach above-mentioned purpose, the utility model embodiment adopts the following technical scheme that：
The utility model embodiment provides a kind of organization engineering skin model culture apparatus, organization engineering skin model culture
Device includes device box body, the device upper cover being arranged on device box body, and culture support plate and multiple culture cells；
Wherein, device box body is used to accommodate culture cell and culture support plate；The bottom of device box body is provided with stent, props up
Frame is used to support device box body；Multiple contour pillars are provided with the inside of device box body, pillar is used to support culture support plate；
Liquid filling hole and liquid feeding port lid are provided with lid on device, divides row handle hole and point row operation port lid, and auxiliary handle
Hand, wherein, nutrient solution is added for device box body by liquid filling hole, divides row handle hole corresponding culture by a point row operation hole operation
Cell, auxiliary handle are used for opening device upper cover.
Further, the circular hole that multiple support cells are provided with support plate is cultivated, supports the circular hole of cell is used to support to put
Enter culture cell therein.
Further, the height of stent makes bottom surface of the bottom of culture cell away from device box body have the distance of 2~8mm.
Further, upper part of the cover edge is equipped with the card slot with the support assorted of device box body on device, makes device
Box body can be fitted together to be stuck in fix above device upper cover and stack, and make penetrating between device box body and device upper cover, guarantee air
Circulation.
Further, the spacing of the circular hole of multiple support cells is 2~3cm, is to engrave between the circular hole of multiple support cells
Hollow structure, assembles to reduce culture support plate and cultivate the nutrient solution between cell.
Further, cultivate and multiple vertical columns are additionally provided with support plate, the height of vertical columns and the insertion of culture cell
The depth of circular hole should make culture cell bottom have the distance of 2~8mm away from box bottom, and cell bottom and device box are cultivated to eliminate
The nutrient solution aggregation that body bottom surface is formed by surface tension effects.
Further, device box body is cuboid.
The utility model embodiment provides a kind of organization engineering skin model culture apparatus, including device box body, is arranged on
Device upper cover on device box body, and culture support plate and multiple culture cells；Device box body is used to accommodate culture cell and training
Support support plate；Stent is provided with the bottom of device box body, stent is used to support device box body；It is provided with the inside of device box body more
A contour pillar, pillar are used to support culture support plate；Liquid filling hole and liquid feeding port lid are provided with lid on device, divides row handle hole
With a point row operation port lid, and auxiliary handle, nutrient solution is added for device box body by liquid filling hole, passes through a point row operation hole operation
Divide the corresponding culture cell of row handle hole, auxiliary handle is used for opening device upper cover.
Description based on above-described embodiment, compared with the prior art, the utility model has the advantage that：
（1）Equipped with independent tegillum, avoid the area that operating process is exposed in air excessive, dirt can be reduced to greatest extent
Contaminate risk.
（2）Single-row independent operation, convenient operation, the pollution risk that can also avoid more hole operations from introducing.
（3）Culture support plate contains independent column, can avoid, because branch plate suqare is excessive, can not carrying excessive cell and becoming
Shape, influences gas-liquid face condition of culture, causes organization engineering skin structure quality unstable or fails.
（4）Auxiliary handle of uncapping is set up, it is convenient to open upper cover, improve operating efficiency.
（5）The large-scale production and test of organization engineering skin model can be achieved using this setting, and can be to greatest extent
Pollution risk is reduced, ensures the success rate and quality stability of model construction.
Brief description of the drawings
Fig. 1 is a kind of structure diagram for organization engineering skin model culture apparatus that the utility model embodiment provides
One；
Fig. 2 is a kind of structure diagram for organization engineering skin model culture apparatus that the utility model embodiment provides
Two；
Fig. 3 is a kind of structure diagram for organization engineering skin model culture apparatus that the utility model embodiment provides
Three；
Fig. 4 is a kind of structure diagram for organization engineering skin model culture apparatus that the utility model embodiment provides
Four；
Wherein, 1 is device upper cover, and 2 be liquid filling hole, and 3 be auxiliary handle, and 4 is divide row handle hole, and 5 be device box body, and 6 are
Stent, 7 be pillar, 8 between two stands notch, 9 be liquid filling hole lower zone, and 10 be culture support plate, and 11 is support the circle of cell
Hole, 12 be hollow part, and 13 be culture cell, and 14 be the vertical columns on support plate, and 15 be a point row operation port lid, and 16 be liquid filling hole
Lid.
Embodiment
The following is a combination of the drawings in the embodiments of the present utility model, and the technical scheme in the embodiment of the utility model is carried out
Describe in detail.
Each component of organization engineering skin model culture apparatus designed by the utility model can use plastic injected die to be molded,
It is Thermo Fischer Scient Inc. wherein to cultivate cell（China）Sale（Article No. 140720）.Box lid and handle shapes can be according to realities
Border needs to be adjusted.
Embodiment 1
The utility model embodiment provides a kind of organization engineering skin model culture apparatus, as shown in Figs 1-4, including box
Body, upper cover, independent tegillum, liquid feeding port lid, culture support plate and culture cell；It is characterized in that, the box body is rectangle,
Pillar is equipped with the inside of box body, strut height should make culture cell bottom have the distance of 2~8mm away from box bottom, coordinate culture branch
Contour column on plate, is used to support culture support plate；Side sets liquid-changing hole in box body, changes bottom and the opposite side of liquid zone
Cultivation region bottom connects；The edge on box body top can coordinate with the bottom of upper cover, make upper cover fastening lid on box body, on upper lid
The edge in portion is equipped with the card slot coordinated with box body bottom bracket, box body is fitted together to and is stuck in fixation above upper cover and stacks,
And make penetrating guarantee air circulation between box body and upper cover；It is opposite with changing liquid zone in box body that liquid-changing hole is equipped with the side of upper cover
Should, liquid-changing hole is equipped with matched liquid-changing hole lid；Upper cover liquid-changing hole homonymy fills handle of uncapping, convenient overall unlatching；Corresponding culture
Each column position of support plate designs independent tegillum, and height is adapted to box body outer bottom, box body is fixed and stack；The culture support plate
Geomery is consistent with cultivation region in box body, and box body pillar and the upright supports cultivated on support plate can be relied on to lie in a horizontal plane in training
Support in area, cultivate and some circular holes for placing culture cell are set in order on support plate, culture cell is embedded in circular hole, realize training
Foster support plate supports culture cell and is suspended from above cultivation region；The spacing of circular hole is 2~3cm, is engraved structure between circular hole,
Assemble to reduce culture support plate and cultivate the nutrient solution between cell；The height of column and the depth of culture cell insertion circular hole
Culture cell bottom should be made to have the distance of 2~8mm away from box bottom, to eliminate culture cell bottom with box bottom because of surface
Power acts on formed nutrient solution aggregation.
The application method of the skin test model culture apparatus of the utility model, opens upper cover, and culture support plate is placed in training
Support in device box body, then inserted cell is cultivated one by one in the circular hole of culture support plate（Culture support plate on can set circular hole 16~
96）；After covering upper cover, when cultivating small indoor inoculation cell, the corresponding independent tegillum in top can be opened by row, divides row to be grasped
Make, some culture apparatuses can be stacked to be placed in incubator after being fully completed and be cultivated；In gas-liquid incubation, it can also lead to
Cross the corresponding tegillum of unlatching and carry out cell operation；It is whole that liquid is changed by liquid-changing hole injection nutrient solution or absorption, until completing tissue work
Journey skin model is built.In the subsequent testing operation of organization engineering skin, it can also be carried out according to aforesaid operations mode, Ke Yibao
The homogeneity of test condition is demonstrate,proved, while the pollution risk that large area exposure can be avoided to introduce to greatest extent.
Exemplary, the utility model embodiment provides a kind of organization engineering skin model culture apparatus, specifically includes
Box body, upper cover, culture support plate and culture cell, box body grow 15.6 cm, wide 12.5 cm, in the corner of box body outer bottom for rectangle
Equipped with stent, the height 1.5cm of stent；Cultivate the tubular container that cell is diameter about 1cm, the uniformly distributed leg of bottom side portion, training
The bottom for supporting cell uses polycarbonate membrane（For pellicle）, nutrient solution can penetrate the film and realize nutrient supply, and raised changes
Liquid vent lid is just covered on liquid-changing hole, and a raised point row operation port lid is covered on row handle hole is divided.Uncap behaviour for liquid filling hole side
Make handle.Support plate long 15.4cm, wide 12.3 cm to be cultivated, there are 24 circular holes for placing culture cell thereon, circular hole is arranged in a straight line,
Circular hole spacing is 20mm, is engraved structure between circular hole, and culture support plate divides into stem height 4mm, in cultivation region box side wall
Height is uniformly arranged as high columns such as 4mm, referring to attached drawing, the user of the skin test model culture apparatus of the utility model
Method, culture support plate is placed in culture apparatus box body, then is inserted cell is cultivated one by one in the circular hole of culture support plate；It is small cultivating
Indoor inoculation cell, covers upper cover and point row operation lid, can stack to be placed in some culture apparatuses in incubator and carry out gas-liquid
Culture；The cultivation stage under liquid, by dividing row handle hole to carry out single-row operation to each cell of the row containing model, in gas-liquid culture
Cheng Zhong, is injected nutrient solution or is drawn and change liquid by liquid-changing hole, until completing the structure of organization engineering skin model.
Organization engineering skin model test in use, open each point of row port lid respectively, surveyed to culture cell inner skin
Test agent is added in die trial type, is tested by established procedure, irritation, corrosivity inspection available for skin test model
Survey.The utility model can ensure each culture cell inner skin test model acceptance test under identical environmental condition, obtain
While obtaining accurate test result data, ensure the homogeneity of model quality to greatest extent, greatly reduce model
The pollution risk introduced in production, operating process.
The utility model embodiment provides a kind of organization engineering skin model culture apparatus, including device box body, is arranged on
Device upper cover on device box body, and culture support plate and multiple culture cells；Device box body is used to accommodate culture cell and training
Support support plate；Stent is provided with the bottom of device box body, stent is used to support device box body；It is provided with the inside of device box body more
A contour pillar, pillar are used to support culture support plate；Liquid filling hole and liquid feeding port lid are provided with lid on device, divides row handle hole
With a point row operation port lid, and auxiliary handle, nutrient solution is added for device box body by liquid filling hole, passes through a point row operation hole operation
Divide the corresponding culture cell of row handle hole, auxiliary handle is used for opening device upper cover.Description based on above-described embodiment, the tissue
Engineering skin model culture apparatus is easy to operation and can reduce pollution risk, while can ensure product quality, and is suitable for scale
Metaplasia produces organization engineering skin.
The above, is only the embodiment of the application, but the protection domain of the application is not limited thereto, any
Change or replacement in the technical scope that the application discloses, should all cover within the protection domain of the application.Therefore, this Shen
Protection domain please should be based on the protection scope of the described claims.
Claims (7)
- A kind of 1. organization engineering skin model culture apparatus, it is characterised in that the organization engineering skin model culture apparatus bag The device upper cover for include device box body, being arranged on described device box body, and culture support plate and multiple culture cells；Wherein, described device box body is used to accommodate culture cell and culture support plate；The bottom of described device box body is provided with branch Frame, the stent are used to support described device box body；Multiple contour pillars, the branch are provided with the inside of described device box body Column is used to support the culture support plate；Liquid filling hole and liquid feeding port lid are provided with lid in described device, divides row handle hole and point row operation port lid, and auxiliary handle Hand, wherein, nutrient solution is added for described device box body by the liquid filling hole, point row described in hole operation are operated by described point of row The corresponding culture cell of handle hole, the auxiliary handle are used to open described device upper cover.
- 2. organization engineering skin model culture apparatus according to claim 1, it is characterised in that set on the culture support plate The circular hole of multiple support cells is equipped with, it is described to support the circular hole of cell is used to support to be put into culture cell therein.
- 3. organization engineering skin model culture apparatus according to claim 1 or 2, it is characterised in that the height of the stent Degree makes the bottom surface of the bottom of cell away from described device box body of cultivating have the distance of 2~8mm.
- 4. organization engineering skin model culture apparatus according to claim 1 or 2, it is characterised in that described device upper cover The edge on top is equipped with the card slot with the support assorted of described device box body, described device box body is fitted together to and is stuck in institute State fixation above device upper cover to stack, and make penetrating between described device box body and described device upper cover, guarantee air circulation.
- 5. organization engineering skin model culture apparatus according to claim 2, it is characterised in that multiple support cells The spacing of circular hole be 2~3cm, be engraved structure between multiple circular holes for supporting cells, to reduce culture support plate and Cultivate the nutrient solution aggregation between cell.
- 6. the organization engineering skin model culture apparatus according to claim 2 or 5, it is characterised in that the culture support plate On be additionally provided with multiple vertical columns, the height of the vertical columns and the depth of culture cell insertion circular hole should make culture cell The distance of 2~8mm is arranged away from box bottom at bottom, to eliminate culture cell bottom with described device box bottom because surface tension is made Assembled with the nutrient solution formed.
- 7. organization engineering skin model culture apparatus according to claim 1 or 2, it is characterised in that described device box body For cuboid.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720706095.8U CN207259519U (en) | 2017-06-18 | 2017-06-18 | A kind of organization engineering skin model culture apparatus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720706095.8U CN207259519U (en) | 2017-06-18 | 2017-06-18 | A kind of organization engineering skin model culture apparatus |
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| Publication Number | Publication Date |
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| CN207259519U true CN207259519U (en) | 2018-04-20 |
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|---|---|---|---|
| CN201720706095.8U Active CN207259519U (en) | 2017-06-18 | 2017-06-18 | A kind of organization engineering skin model culture apparatus |
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| Country | Link |
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| CN (1) | CN207259519U (en) |
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2017
- 2017-06-18 CN CN201720706095.8U patent/CN207259519U/en active Active
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| PE01 | Entry into force of the registration of the contract for pledge of patent right | ||
| PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of utility model: Organizational project skin model culture apparatus Effective date of registration: 20200426 Granted publication date: 20180420 Pledgee: Xianyang corporate finance Company limited by guarantee Pledgor: GUANGDONG BIOCELL BIOTECHNOLOGY Co.,Ltd. Registration number: Y2020980001783 |
|
| PC01 | Cancellation of the registration of the contract for pledge of patent right | ||
| PC01 | Cancellation of the registration of the contract for pledge of patent right |
Date of cancellation: 20210528 Granted publication date: 20180420 Pledgee: Xianyang corporate finance Company limited by guarantee Pledgor: GUANGDONG BIOCELL BIOTECHNOLOGY Co.,Ltd. Registration number: Y2020980001783 |