CN216594438U - Full-automatic high-flux multifunctional dyeing machine - Google Patents

Abstract

The utility model relates to a full-automatic high-flux multifunctional dyeing machine which comprises a base with an accommodating cavity, wherein a TIP head seat for storing a TIP head, a test tube seat for storing a test tube, a dyeing base, a reagent seat for storing a reagent, a washing tank and a mechanical arm capable of moving in three axes are arranged on the upper surface of the base at intervals, an operating mechanism is arranged on the side wall of the base, a control circuit board is arranged on the base, the operating mechanism and the mechanical arm are electrically connected with the control circuit board, and a sampling mechanism is arranged on the mechanical arm. The utility model discloses a can draw when cell film-making dyeing and shift and release the sample solution that contains the cell, other subassemblies are used to follow-up flow and immunohistochemical dyeing compatibility, have realized multi-functional dyeing, still can deposit multiple type simultaneously, and many specifications capacity reagent has realized the high flux according to current actual demand.

Description

Full-automatic high-flux multifunctional dyeing machine

Technical Field

The utility model relates to immunohistochemical dyeing and cell slide dyeing equipment, in particular to a full-automatic high-flux multifunctional dyeing machine.

Background

In the field of pathological diagnosis, the intensity of manual operation is increased due to the gradual rise of the number of experiments in hospitals and the standardization of dyeing technology, so that the whole process is difficult to complete quickly in time, the dyeing time of different reagents is difficult to accurately set, errors exist in manual operation, and the consistency is not high. Although there are many similarities for immunohistochemical staining and cell slide staining processes, different staining requirements in the existing instruments and equipment can only be applied by respective corresponding equipment, and the generalization cannot be achieved, and the resource utilization rate of the instrument is low.

SUMMERY OF THE UTILITY MODEL

Aiming at the existing defects, the utility model provides a full-automatic high-flux multifunctional dyeing machine.

The technical scheme adopted by the utility model for solving the technical problem is as follows: the utility model provides a multi-functional dyeing machine of full-automatic high flux, includes the base that has the holding cavity in, base upper surface interval is provided with a plurality of TIP headstock that are used for depositing the TIP head, a plurality of test tube seats that are used for depositing the test tube, a dyeing base that is used for placing the slide glass, a plurality of reagent seats, washing tank that are used for depositing the reagent, the arm that can the triaxial removal on the base, be provided with control mechanism on the lateral wall of base, be provided with control scheme board on the base, control mechanism and arm all with control scheme board electric connection, be provided with sampling mechanism on the arm.

Preferably, the dyeing base comprises a base bottom plate with a groove formed in the surface and a base cover plate with a sealing cover arranged on the groove, a heating sheet corresponding to the bottom surface of the base cover plate is arranged in the groove, a temperature sensor and a switch for controlling the heating sheet are further arranged in the groove, the heating sheet, the temperature sensor and the switch are all electrically connected with a control circuit board, and a glass slide placing seat for placing a glass slide is arranged on the upper surface of the base cover plate.

Preferably, a water pipe communicated with the base bottom plate is further arranged below the base bottom plate, a drain hole communicated with the water pipe is formed in the base cover plate, a drain groove is formed in the base cover plate around the glass slide placing seat, an atomizer electrically connected with the control circuit board is arranged at the water outlet of the water pipe, a spraying through hole communicated with the atomizer is further formed in the base cover plate, a placing seat groove is formed in the lower portion of the glass slide placing seat, a notch communicated with the drain groove is formed in one side wall of the placing seat groove, and the groove bottom of the placing seat groove is an inclined plane which inclines downwards from one side opposite to the notch to one side where the notch is located.

Preferably, the reagent holders include a single reagent holder for storing a single reagent, a mixed reagent holder for storing a mixed reagent, and an alcohol holder for storing alcohol.

Preferably, the control mechanism is a touch screen.

Preferably, the cleaning tank includes a sample application needle cleaning hole for cleaning the sample application needle, and a plate cleaning needle cleaning hole for cleaning the slide.

Preferably, the upper surface of the base is further provided with an identification pattern corresponding to the TIP holder, the test tube holder, the staining base, the reagent holder, and the cleaning tank for identifying the positions thereof.

Preferably, the upper surface of the base is further provided with a recovery box for recovering the TIP head.

Preferably, the sampling mechanism comprises a sampling needle arranged on the mechanical arm and a liquid level detection sensor arranged on the sampling needle.

Preferably, the upper surface of the base is further provided with a moisturizing cover which can be detachably arranged on the upper surface of the base and is used for moisturizing.

The utility model has the beneficial effects that: the utility model discloses an utilize TIP headstock and test tube seat, can draw when the cell film-making is dyed and shift and release the sample solution that contains the cell, follow-up flow and other subassemblies of compatible use of immunohistochemical dyeing, multi-functional dyeing has been realized, multiple type can be deposited to the reagent position seat simultaneously, the reagent of many specification capacity, the slide on the dyeing base, can be according to current actual demand, high flux has been realized, realize the general of specific immunohistochemical dyeing and cell film-making dyeing flow equipment, it is many to have solved the dyeing experiment among the prior art, it is difficult to standardize, need to deal with the problem that different dyeing requirements used different equipment, the automation has been realized, high flux, the technological effect of multi-functional dyeing.

Drawings

FIG. 1 is a schematic structural diagram of an embodiment of the present invention;

FIG. 2 is an exploded view of a dyeing base according to an embodiment of the present invention;

FIG. 3 is a schematic view of a base cover structure according to an embodiment of the present invention;

FIG. 4 is a schematic diagram of the structure of each part on the upper surface of the base according to the embodiment of the present invention;

FIG. 5 is a schematic view of a robotic arm according to an embodiment of the present invention;

part names and serial numbers in the figure: 1-base 10-identification pattern 11-recovery box 12-moisturizing cover 2-TIP headstock 3-test tube seat 4-dyeing base 40-glass slide 41-base bottom plate 42-base cover plate 43-heating plate 44-temperature sensor 45-switch 420-glass slide placing seat 421-drain hole 422-drain groove 423-spray through hole 424-placing seat groove 425-notch 5-reagent seat 50-single reagent seat 51-mixed reagent seat 52-alcohol seat 6-washing groove 7-mechanical arm 8-control mechanism 9-sampling mechanism.

Detailed Description

To more clearly illustrate the objects, technical solutions and advantages of the embodiments of the present invention, the present invention will be further described in detail with reference to the accompanying drawings and embodiments, and it is obvious that the described embodiments are some, but not all embodiments of the present invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments of the present invention without inventive step, are within the scope of the present invention. In addition, directional terms used in the present invention, such as "upper", "lower", "front", "rear", "left", "right", "inner", "outer", etc., refer to directions only as shown in the attached drawings, and are used for better and clearer explanation and understanding of the present invention, and do not indicate or imply orientation which the present invention must have, and thus, should not be construed as limiting the present invention.

Embodiment of the utility model as shown in fig. 1 to 5, a fully automatic high-flux multifunctional dyeing machine comprises a base 1 with a containing cavity, wherein the base 1 forms a machine shell of the dyeing machine and is used for installing various functional components, a plurality of TIP headstock 2 for storing TIP heads, a plurality of test tube seats 3 for storing test tubes, a plurality of dyeing bases 4 for placing glass slides 40, a plurality of reagent seats 5 for storing reagents, a cleaning tank 6 and a mechanical arm 7 capable of moving three shafts above the base are arranged on the upper surface of the base 1 at intervals, according to the using habit, the reagent seats 5 can be arranged at the leftmost side, the reagent seats 4 are arranged next to the reagent seats 5, the right side of the dyeing bases 4 is the test tube seats 3 and the TIP headstock 2, the TIP headstock 2 is positioned behind the test tube seats 3, the cleaning tank 6 is also arranged at the left side of the dyeing bases 4 and behind the reagent seats 5, meanwhile, the mechanical arm 7 is arranged at the rear end of the base 1 and moves three-axially above the base 1 as required, and at the moment, the TIP headstock 2 can be arranged into TIP headstock for placing TIP heads with different specifications according to the use requirement, each specification can be arranged into a plurality of TIP headstock, or the TIP headstock 2 for placing TIP heads with the same specification can be arranged, for example, 42 TIP headstock with the specification of 1ml are arranged, and the TIP headstock with single specification or multiple specifications are arranged on the base 1 in a row-column matrix structure, so that the use is convenient; the test tube seats 3 can also be arranged into test tube seats for placing test tubes of different specifications or test tubes of the same specification according to the use requirements, and are also arranged in a matrix structure in rows and columns, for example, the test tube seats 3 of 42 test tubes of 15ml in a single specification are arranged, and the sample solution containing cells is placed in the test tubes; the dyeing bases 4 are positioned in the middle of the base 1, operation and use are convenient, a plurality of the dyeing bases are arranged on the base 1 according to the row-column structure, each dyeing base 4 is provided with a glass slide 40 for dyeing, the glass slide 40 can be used for dripping sample solution or various reagents, cleaning solution, pure water and the like, at the moment, the number of the dyeing bases 4 can be set to 6, each dyeing base 4 is set to a base capable of placing 7 glass slides 40 for dyeing, and high-flux operation can be carried out; the reagent seats 5 are also provided with reagent seats 5 for placing reagents with different specifications or reagent seats 5 for placing reagents with the same specification according to the use requirements, and are also provided with a matrix structure in rows and columns, for example, 10 reagent seats with 15ml and 30 reagent seats with 1.5ml, the reagents required for dyeing are placed in containers with corresponding volumes, then the containers are placed in the reagent seats with the corresponding specifications, meanwhile, in order to meet the use requirements of mixed reagents, the reagent seats 5 comprise a single reagent seat 50 for storing single reagents, a mixed reagent seat 51 for storing mixed reagents and an alcohol seat 52 for storing alcohol, the single reagent seat 50 is used for placing single pure reagents, a large number of reagent seats can be provided, the mixed reagent seat 51 is used for storing the mixed reagents or a plurality of single reagents are mixed in the mixed reagent seat 51 and then stored, and a temporary storage function is achieved, a relatively small number, for example 2, can be set, and the alcohol seat 52 is used for storing alcohol, for example, 500ml alcohol bottles, so that the operator can add alcohol conveniently during the operation process; the cleaning tank 6 is a cleaning blind hole arranged on the base 1 and comprises a sample adding needle cleaning hole for cleaning a sample adding needle and a plate cleaning needle cleaning hole for cleaning a glass slide, and the cleaning tanks can be used for cleaning the sample adding needle and the plate cleaning needle independently or simultaneously; the side wall of the base 1 is provided with an operation mechanism 8, the operation mechanism 8 is used for operating the operation of the mechanical arm 7, the operation can be performed manually, an operation program burnt on a control circuit board can be used for controlling the operation of the mechanical arm 7, the operation mechanism 8 is preferably a touch screen, the use is convenient, at the moment, the upper surface of the base 1 is further provided with an identification pattern 10 which corresponds to the TIP headstock 2, the test tube seat 3, the dyeing base 4, the reagent seat 5 and the washing tank 6 and is used for identifying the position of the mechanical arm, the operation instruction of the mechanical arm 7 is reached up and down on the touch screen, the operation instruction sets the coordinates of each part through a preset program, then the mechanical arm 7 moves in three axes directions according to the coordinates by using the control of the control circuit board until the specified position is reached, at the moment, the movement of the mechanical arm 7 in the three axes directions is that the mechanical arm 7 moves along X, the motion of Y, Z axle direction sets up 2 guide rails in Y axle direction, but drags Z axle direction vertical drive application of sample needle, wash the board needle, the part of alcohol needle and TIP head and moisturizing cover seat and snatchs mechanical device respectively, still sets up 2 tow chains, place the motor pencil that drags Z axle direction motion in the tow chain, liquid level detection pencil, and reagent, alcohol, wash the board pipeline etc., be provided with the control scheme board on the base 1, can be according to different user demands, set up it in base 1 or any direction outside base 1, such as the side rear of base 1 or the left and right sides of base 1 etc., the control scheme board just is as the control center of this dyeing machine, can set up MCU on it to issue the instruction and receive the information that analysis each functional unit sent back to each part of operation of each part, control mechanism 8 and arm 7 all with control scheme board electric connection, the sampling mechanism 9 is arranged on the mechanical arm 7, the mechanical arm 7 carries out sampling and sample adding through the sampling mechanism 9 on the mechanical arm after moving to a specified position through three shafts, the plate is washed, alcohol is added and the like, the sampling mechanism 9 comprises a sampling needle arranged on the mechanical arm 7 and a liquid level detection sensor arranged on the sampling needle, the sampling needle absorbs a reagent and a sample solution and is added on a glass slide on a dyeing base, the liquid level detection sensor is used for judging the amount of a reagent in a reagent seat, a subsequent sample adding absorbing program is carried out after the sufficient amount of the reagent is judged, if a small-size liquid level detection plate is connected at the shortest distance position of the reagent sample adding needle, the detection of the amount of the reagent is realized, and meanwhile, the sampling mechanism 9 further comprises a plate washing needle for absorbing a cleaning liquid to wash the glass slide 40 and the dyeing base 4, an alcohol needle for absorbing alcohol and the like. Meanwhile, the upper surface of the base 1 is also provided with a recovery box 11 for recovering the TIP head, so that the TIP head waste is conveniently treated. When dyeing is carried out, setting and selecting a flow on a touch screen, placing a reagent bottle before dyeing, placing a glass slide 40 on a dyeing base 4, setting the flow and parameters, moving a mechanical arm 7 to a designated reagent bottle, moving a sampling needle on the mechanical arm 7, judging the amount of a reagent through liquid level detection, returning to a cleaning tank to clean the needle, judging the sufficient amount of the reagent, sucking the reagent according to the flow, moving the reagent to the glass slide 40 on the dyeing base 4, or adding a plurality of reagents to a mixed reagent seat to mix uniformly, transferring the mixed reagent to the glass slide 40 on the dyeing base 4, moving the reagent to the cleaning tank 6 after each reagent or reagent is added for detection in the whole process, cleaning the sampling needle, simultaneously cleaning the plate-washing needles when needed, moving the plate-washing needles to the cleaning tank 6 after each class of cleaning is executed, cleaning the plate-washing needles when needed, and simultaneously cleaning the reagent needles when needed, the cleaning mode is that the mechanical arm 7 moves the plate washing needle to the glass slide 40, and the plate washing needle is placed downwards for cleaning. When cell slide staining is carried out, the mechanical arm 7 can take the TIP head from the TIP headstock 2 to the test tube seats 3 corresponding to the serial numbers to suck samples, then the samples move to the positions of the slide glass 40 corresponding to the serial numbers, the TIP head releases sample solution, after release, the samples move to the TIP head recovery box 11 to drop the TIP head, then the alcohol needle releases alcohol on the slide glass to carry out cell fixation, and then the staining procedure is similar to the staining procedure.

In a further improvement, as shown in fig. 1 to 4, the dyeing base 4 includes a base bottom plate 41 with a groove on the surface, a base cover plate 42 with a sealing cover on the groove, the base cover plate 42 is made of an aluminum alloy metal block, the sealing cover is arranged on the groove to form a closed space inside the groove, the sealing is realized by arranging a waterproof rubber sealing ring between the two, the space can be used for arranging some parts, the groove is internally provided with a heating plate 43 corresponding to the bottom surface of the base cover plate 42, the groove is also internally provided with a temperature sensor 44 and a switch 45 for controlling the heating plate 43, the temperature sensor 44 and the switch 45 are all electrically connected with the control circuit board, the upper surface of the base cover plate 42 is provided with a slide placing seat 420 for placing a slide 40, and the slide placing seat 420 can be heated by the heating plate 43, and then the slide glass 40 placed on the temperature sensor 44 is heated to meet the temperature required during dyeing, the temperature sensor is used for collecting the temperature of the slide glass placing seat 420, when the temperature is too high, the switch 45 stops the operation of the heating plate 43, and at this time, the switch 45 is a temperature protection switch connected with the heating plate 43 in series. The upper surface of the base 1 is also provided with a moisturizing cover 23 which can be detachably arranged on the upper surface of the base 2 and is used for moisturizing, the upper surface of the base 2 is provided with a groove or a protrusion, so that the moisturizing cover 23 can be detachably clamped in the groove or on the protrusion, the bottom surface of the moisturizing cover 23 is set to be matched with the upper surface of the single dyeing base 4, the moisturizing cover 12 can completely cover the glass slide 40 placed on the single dyeing base 4 to preserve heat, 2 glass slides can be arranged as required, the use is convenient, the moisturizing function is realized when the dyeing base 4 is grabbed by the mechanical arm 7 to dye when the base is used, a water pipe which is communicated with the lower part of the base bottom plate 1 is also arranged below the base bottom plate 42, the base cover plate 42 is provided with a drain hole 421 communicated with the water pipe, the base cover plate 42 is provided with a drain groove around the glass slide placing seat 420, and the water outlet of the water pipe is provided with an atomizer which is electrically connected with the control circuit board, the base cover plate 42 is further provided with a spraying through hole 423 communicated with the atomizer, the lower part of the slide glass placing seat 420 is provided with a placing seat groove 424, one side wall of the placing seat groove 424 is provided with a notch 425 communicated with the drainage groove, the bottom of the placing seat groove 424 is an inclined plane which is inclined downwards from one side opposite to the notch 425 to one side where the notch 425 is located, when the glass slide placing seat is used, a water pipe is used for communicating an external water source, the atomizer which is discharged from a water outlet of the water pipe atomizes water and then sprays the water through the spraying through hole 423, therefore, the atomizer can be used for atomizing the water regularly and then spraying the slide glass 40 on the dyeing base 4 through the spraying through hole 423, the wet environment of the dyeing base 4 is kept, and the drainage groove 422 and the notch 425 can discharge redundant water from the dyeing base 4.

It will be understood that modifications and variations can be made by persons skilled in the art in light of the above teachings and all such modifications and variations are intended to be included within the scope of the utility model as defined in the appended claims.

Claims (10)

1. The full-automatic high-flux multifunctional dyeing machine is characterized by comprising a base with a containing cavity, wherein a plurality of TIP head seats for storing TIP heads, a plurality of test tube seats for storing test tubes, a plurality of dyeing bases for placing glass slides, a plurality of reagent seats for storing reagents, a washing tank and a mechanical arm capable of moving in a three-axis mode are arranged on the upper surface of the base at intervals, an operating mechanism is arranged on the side wall of the base, a control circuit board is arranged on the base, the operating mechanism and the mechanical arm are electrically connected with the control circuit board, and a sampling mechanism is arranged on the mechanical arm.

2. The full-automatic high-flux multifunctional dyeing machine as claimed in claim 1, wherein the dyeing base comprises a base bottom plate with a groove on the surface, a base cover plate with a sealing cover on the groove, a heating plate corresponding to the bottom surface of the base cover plate is arranged in the groove, a temperature sensor and a switch for controlling the heating plate are arranged in the groove, the heating plate, the temperature sensor and the switch are all electrically connected with a control circuit board, and a slide placing seat for placing a slide is arranged on the upper surface of the base cover plate.

3. The full-automatic high-flux multifunctional dyeing machine as claimed in claim 2, wherein a water pipe is further provided under the base bottom plate, a water drain hole is provided on the base cover plate, the water drain groove is provided on the base cover plate around the slide glass placing seat, an atomizer electrically connected to the control circuit board is provided at a water outlet of the water pipe, a spray through hole is further provided on the base cover plate, the atomizer is communicated with the spray through hole, a placing seat recess is provided on the lower portion of the slide glass placing seat, a notch communicated with the water drain groove is provided on one side wall of the placing seat recess, and a groove bottom of the placing seat recess is an inclined surface inclined downward from a side opposite to the notch to a side where the notch is located.

4. The full-automatic high-throughput multifunctional dyeing machine according to claim 1, wherein said reagent seat comprises a single reagent seat for storing single reagent, a mixed reagent seat for storing mixed reagent, and an alcohol seat for storing alcohol.

5. The full-automatic high-flux multifunctional dyeing machine according to claim 1, wherein the control mechanism is a touch screen.

6. The full-automatic high-throughput multifunctional dyeing machine according to claim 1, wherein the cleaning tank comprises a loading needle cleaning hole for cleaning the loading needle, and a plate cleaning needle cleaning hole for cleaning the slide.

7. The full-automatic high-flux multifunctional dyeing machine as claimed in claim 1, wherein the upper surface of said base is further provided with identification patterns corresponding to the TIP headstock, the test tube seat, the dyeing base, the reagent seat and the washing tank for identifying the positions thereof.

8. The fully automatic high flux multifunctional dying machine according to claim 1, characterised in that the upper surface of said base is also provided with a recovery box for recovering the TIP head.

9. The full-automatic high-flux multifunctional dyeing machine according to claim 1, wherein the sampling mechanism comprises a sampling needle disposed on the mechanical arm, and a liquid level detection sensor disposed on the sampling needle.

10. The full-automatic high-flux multifunctional dyeing machine as claimed in claim 1, wherein said base is further provided at its upper surface with a moisture-keeping cover for keeping moisture detachably attached to the upper surface of the base.

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