CN216473239U

CN216473239U - Vortex oscillation device

Info

Publication number: CN216473239U
Application number: CN202122462244.XU
Authority: CN
Inventors: 罗深恒; 孙国栋; 陈�光
Original assignee: Guangzhou Gaosheng Zhizao Technology Co ltd
Current assignee: Guangzhou Gaosheng Zhizao Technology Co ltd
Priority date: 2021-10-13
Filing date: 2021-10-13
Publication date: 2022-05-10
Anticipated expiration: 2031-10-13

Abstract

The utility model discloses a vortex oscillation device, which belongs to the technical field of oscillation devices, and comprises: an oscillating assembly comprising an oscillating platform capable of eccentric rotational motion; the sample loading plate placing platform comprises a plurality of sample loading plate placing platforms, wherein the sample loading plate placing platforms are arranged on the oscillation platform in an array mode, each sample loading plate placing platform can be used for placing one sample loading plate, and each sample loading plate placing platform is provided with a limiting block for limiting the sample loading plates on the sample loading plate placing platforms. The utility model can rapidly complete DNA collection of a large batch of samples.

Description

Vortex oscillation device

Technical Field

The utility model relates to the technical field of oscillation devices, in particular to a vortex oscillation device.

Background

When DNA is extracted by a magnetic bead method or a silica bead method, a vortex oscillator is adopted to carry out vortex oscillation on a sample solution.

In the prior art, the vortex oscillator can only carry out vortex oscillation on one sample bearing plate at a time. When the number of samples to be collected is large, the consumed time is long, and the DNA collection of a large number of samples cannot be completed quickly.

Therefore, a vortex oscillation device is needed to solve the above problems.

SUMMERY OF THE UTILITY MODEL

The utility model aims to provide a vortex oscillation device which can rapidly finish DNA collection of a large batch of samples.

As the conception, the technical scheme adopted by the utility model is as follows:

a vortex oscillation apparatus comprising:

an oscillating assembly comprising an oscillating platform capable of eccentric rotational movement;

the sample loading plate placing table comprises a plurality of sample loading plate placing tables, wherein the sample loading plate placing tables are arrayed on the oscillation platform, each sample loading plate placing table can be used for placing one sample loading plate, and each sample loading plate placing table is provided with a limiting block for limiting the sample loading plate on the sample loading plate placing table.

Optionally, each sample carrier plate placing table is provided with a heating assembly, and the heating assembly is used for heating the sample carrier plate on the sample carrier plate placing table.

Optionally, the heating assembly comprises a heating plate, the heating plate being capable of generating heat.

Optionally, a heat conducting plate is arranged above the heating plate, and the heat of the heating plate is transferred to the sample bearing plate through the heat conducting plate.

Optionally, a heat insulation plate is arranged below the heating plate.

Optionally, the limiting block includes a first limiting portion and a second limiting portion that are perpendicular to each other, so as to limit the sample carrier plate.

Optionally, the first limiting part and the second limiting part are both provided with an elastic buffer block, and the elastic buffer block can elastically collide with the sample bearing plate.

Optionally, the elastic buffer block includes a buffer housing and an elastic buffer sheet clamped in the buffer housing.

Optionally, the vortex oscillation device further includes a fixed plate, and the oscillation platform is disposed on the fixed plate and can make eccentric rotation motion relative to the fixed plate.

Optionally, the oscillating assembly further comprises:

a drive member;

and one end of the eccentric shaft is fixedly connected with the output end of the driving piece, the other end of the eccentric shaft penetrates through the fixing plate to be connected with the oscillating platform, and the eccentric shaft can drive the oscillating platform to do eccentric rotation motion.

According to the vortex oscillation device provided by the utility model, the sample bearing plate placing tables are arranged on the oscillation platform, so that the vortex oscillation device can finish DNA collection of samples on the sample bearing plates at one time, and the DNA collection speed of a large batch of samples is improved. Meanwhile, a limiting block is arranged on each bearing plate placing table to limit the sample bearing plates, so that the sample bearing plates are prevented from being unstable in oscillation in the oscillation process.

Drawings

FIG. 1 is a schematic view of the overall configuration of a vortex oscillation apparatus provided by an embodiment of the present invention in use;

FIG. 2 is a top view of the vortex oscillation device provided by the embodiment of the present invention without a sample carrier plate;

fig. 3 is a schematic structural diagram of the vortex oscillation device provided by the embodiment of the utility model with a part of the limiting block hidden;

FIG. 4 is a schematic structural diagram of a limiting block according to an embodiment of the present invention;

FIG. 5 is a schematic view of the structure of the elastic buffer sheet of FIG. 4;

FIG. 6 is a schematic view of the assembly of the eccentric shaft with the fixed plate and the oscillating platform provided by the embodiment of the present invention;

FIG. 7 is a schematic structural diagram of an eccentric shaft provided in an embodiment of the present invention;

FIG. 8 is a schematic view of a fixed plate and an oscillating platform provided by an embodiment of the present invention when they are not assembled together;

FIG. 9 is a schematic view of another perspective of the oscillating platform of FIG. 8;

fig. 10 is a schematic structural diagram of a limiting column according to an embodiment of the present invention.

In the figure:

1. an oscillating assembly; 11. a fixing plate; 111. a limiting column; 1111. a first flange; 1112. a second flange; 12. a drive member; 13. an eccentric shaft; 131. a first shaft portion; 132. a second shaft portion; 133. a third shaft portion; 134. a mounting cavity;

2. a heating assembly; 21. a heating plate; 24. a heat insulation plate;

3. a sample carrier plate;

4. an oscillating platform; 41. a first cylindrical cavity; 42. a second cylindrical cavity; 43. a limiting block; 431. an elastic buffer block; 4311. a buffer housing; 4312. an elastic buffer sheet; 43121. a first part; 43122. a second section; 43123. a third section; 432. a first limiting part; 433. a second limiting part;

5. a buffer structure; 51. a raised post; 52. a buffer ring;

61. a first bearing; 62. a second bearing; 63. a first bearing end cap; 64. a first annular portion; 65. a second annular portion;

8. the sample carrying plate is placed on the stage.

Detailed Description

In order to make the technical problems solved, the technical solutions adopted and the technical effects achieved by the present invention clearer, the technical solutions of the present invention are further described below by way of specific embodiments with reference to the accompanying drawings. It is to be understood that the specific embodiments described herein are merely illustrative of the utility model and are not limiting of the utility model. It should be further noted that, for the convenience of description, only some but not all of the elements associated with the present invention are shown in the drawings.

In the description of the present invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc., indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and simplicity of description, but do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus, should not be construed as limiting the present invention. Furthermore, the terms "first," "second," and the like are used for descriptive purposes only and are not to be construed as indicating or implying relative importance. Wherein the terms "first position" and "second position" are two different positions.

In the description of the present invention, it should be noted that unless otherwise explicitly stated or limited, the terms "mounted," "connected," and "connected" are to be construed broadly, e.g., as meaning either a fixed connection or a removable connection; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meanings of the above terms in the present invention can be understood in specific cases to those skilled in the art.

Referring to fig. 1 to 10, the present embodiment provides a vortex oscillation apparatus, which can perform vortex oscillation on a plurality of sample bearing plates simultaneously when DNA is collected by using a magnetic bead method or a silica bead method, so as to rapidly complete DNA collection of a large number of samples.

Specifically, referring to fig. 1 and 2, the vortex oscillation apparatus includes an oscillation assembly 1 and a plurality of sample carrier plate holding stages 8. The oscillating assembly 1 comprises an oscillating platform 4, and the oscillating platform 4 can perform eccentric rotation motion; a plurality of sample loading board are placed the platform 8 and are arranged on the vibration platform 4 in array, and each sample loading board is placed platform 8 and can be placed a sample loading board 3, all is provided with stopper 43 on each sample loading board is placed the platform 8 and carries on spacingly to sample loading board 3 on the platform 8 is placed to the sample loading board.

In particular, in the present embodiment, the sample carrier plate 3 is used for carrying the sample to be collected, and may be a 96-well plate, for example.

Specifically, in this embodiment, four sample bearing plate placing tables 8 are provided on the oscillation platform 4, and the four sample bearing plate placing tables 8 are arranged in a matrix of "2 × 2". Of course, in other embodiments, the plurality of sample carrier placement stations 8 may be arranged in other arrays, such as other rectangular arrays or circular arrays.

Specifically, each sample carrier plate placement stage 8 includes four stopper blocks 43, and the four stopper blocks 43 are located at four corners of the sample carrier plate placement stage 8, respectively.

The stopper 43 includes a first stopper portion 432 and a second stopper portion 433 which are perpendicular to each other to limit the position of the sample carrier plate 3.

In order to prevent the sample bearing plate 3 from being damaged by too large collision force with the limiting block 43 during the oscillation process, the limiting block 43 is further provided with an elastic buffer block 431 to buffer the collision between the sample bearing plate 3 and the limiting block 43. The elastic buffer 431 is located inside the stopper 43 and buffers the collision between the sample carrier plate 3 and the stopper 43 during the oscillation process.

Preferably, the first stopper portion 432 and the second stopper portion 433 are both provided with an elastic buffer 431, and the elastic buffer 431 can elastically collide with the sample carrier plate 3.

Referring to fig. 4 and 5, in the present embodiment, the elastic buffer block 431 includes a buffer housing 4311 and an elastic buffer sheet 4312 clamped inside the buffer housing 4311.

Specifically, the elastic buffer sheet 4312 is made of spring steel, and includes a first portion 43121, a second portion 43122 and a third portion 43123 connected in sequence, where the first portion 43121 and the third portion 43123 are disposed at two opposite ends of the second portion 43122 and both form an obtuse angle with the second portion 43122, and the first portion 43121 and the third portion 43123 extend toward a direction close to the sample carrier plate 3. The ends of the first portion 43121 and the third portion 43123 far from the second portion 43122 are provided with hooks for engaging with the buffer housing 4311.

The second portion 43122 is provided with a connection hole through which a bolt or a screw passes to connect with the stopper 43.

In order to further improve the DNA collection speed to the sample, in this embodiment, each sample loading board is placed and all is provided with heating element 2 on the platform 8, and heating element 2 is used for placing sample loading board 3 on the platform 8 to the sample loading board and heats, can vibrate the magnetic bead fast, improves sampling speed.

Specifically, in the present embodiment, the heating assembly 2 includes a heating sheet 21 and a heat conductive plate.

The heating sheet 21 is provided with a heating wire therein to generate heat. The heat conductive plate is superposed above the heating sheet 21, and the heat of the heating sheet 21 is transferred to the sample carrier plate 3 via the heat conductive plate. The heat conducting plate can transfer heat more uniformly, and is favorable for rapidly extracting the sample DNA in the sample bearing plate 3.

Optionally, the heating assembly 2 further comprises a thermostat to control the temperature of the heating assembly 2. Of course, in other embodiments, the heating assembly 2 may be provided with a temperature sensor to monitor the temperature of the heating assembly 2 in real time.

Specifically, in the present embodiment, the thermostat is electrically connected to the heating sheet 21. The temperature sensor may be disposed on the heating sheet 21 to monitor the temperature of the heating sheet 21 in real time. The temperature controller is used for controlling the temperature of the heating sheet 21.

Of course, in other embodiments, the temperature sensor may also be disposed on the heat conducting plate to monitor the temperature of the heat conducting plate in real time, so as to detect the temperature of the sample bearing plate more accurately.

Further, in order to prevent heat loss, the heating assembly in this embodiment further includes a heat insulation board 24. Specifically, the heat insulation plate 24 is disposed below the heating sheet 21 to prevent heat dissipation and ensure the heating effect of the heating assembly 2 on the sample bearing plate 3.

In this embodiment, one heating element 2 is disposed on each sample plate placing stage 8, and when all sample plate placing stages 8 are not required, one or some sample plate placing stages 8 are required, and only the corresponding heating element 2 is required to be turned on, and not all the heating elements are required to be turned on, thereby avoiding energy waste.

Referring to fig. 6-10, further, the vortex oscillation device further includes a fixed plate 11, and the oscillation platform 4 is disposed on the fixed plate 11 and the oscillation platform 4 can perform eccentric rotation motion with respect to the fixed plate 11. In use of the vortex oscillation apparatus, the fixing plate 11 may be fixed to the frame to provide a support platform for the vortex oscillation apparatus. In the working process, the oscillating platform 4 can perform eccentric rotation motion relative to the fixed plate 11, so that the vortex oscillation of the sample bearing plate 3 is realized.

Referring to fig. 8 and 9, in the present embodiment, a limiting column 111 is disposed on the fixing plate 11, a first cylindrical cavity 41 matched with the limiting column 111 is disposed on the lower surface of the oscillating platform 4, and the limiting column 111 can rock in the first cylindrical cavity 41. When the oscillating platform 4 performs eccentric rotation, the limit column 111 can limit the oscillating platform 4, so as to prevent the oscillating platform 4 from exceeding a limit position due to inertia.

Further, referring to fig. 10, in this embodiment, two ends of the limiting column 111 are respectively provided with a first flange 1111 and a second flange 1112, the fixing plate 11 is provided with a limiting column mounting cavity, a lower half portion of the limiting column 111 is located in the limiting column mounting cavity, and the second flange 1112 is clamped in a second flange clamping groove in the limiting column mounting cavity, an upper half portion of the limiting column 111 is located in the first cylindrical cavity 41, and a diameter of the first cylindrical cavity 41 is greater than a diameter of the second flange 1112, so as to ensure that the limiting column 111 can rock in the first cylindrical cavity 41. Meanwhile, the arrangement of the second flange 1112 enables the upper end of the limiting column 111 to be abutted to the oscillating platform 4 more stably, so that the moving stability of the oscillating platform 4 is ensured.

Preferably, two limiting columns 111 are respectively arranged at four corners of the fixing plate 11. When the oscillating platform 4 oscillates at a high speed, the plurality of stopper posts 111 located at the four corners can restrict the respective orientations of the oscillating platform 4.

Further, referring to fig. 8, in order to reduce noise between the oscillation platform 4 and the fixed plate 11, a buffer structure 5 is provided between the oscillation platform 4 and the fixed plate 11.

The cushion structure 5 includes a protrusion post 51 and a cushion ring 52. The protruding column 51 is disposed on the upper surface of the fixing plate 11, and the buffer ring 52 is sleeved on the protruding column 51

Specifically, the cushion ring 52 is made of a flexible cushion material, such as silicone or rubber.

Referring to fig. 9, a second cylindrical cavity 42 matched with the buffer structure 5 is formed in the lower surface of the oscillating platform 4, and when the oscillating platform 4 is mounted on the fixing plate 11, the buffer structure 5 is located in the second cylindrical cavity 42 and the buffer ring 52 can elastically collide with the inner wall of the second cylindrical cavity 42, so that the collision force is reduced, and noise is reduced.

With reference to fig. 1 and 5, further, the oscillating assembly 1 also comprises a driver 12 and an eccentric shaft 13: one end of the eccentric shaft 13 is fixedly connected with the output end of the driving part 12, the other end of the eccentric shaft passes through the fixing plate 11 and is fixedly connected with the oscillating platform 4, and the eccentric shaft 13 can drive the oscillating platform 4 to do eccentric rotation motion.

Optionally. The driving member 12 is a servo motor, and the servo motor can ensure the oscillation frequency and the stability of oscillation.

Specifically, referring to fig. 5 and 6, in order to enable the eccentric shaft 13 to drive the oscillating platform 11 to perform circumferential shaking when rotating, the eccentric shaft 13 includes a first shaft portion 131, a second shaft portion 132 and a third shaft portion 133, and the axes of the first shaft portion 131, the second shaft portion 132 and the third shaft portion 133 are not overlapped.

The eccentric shaft 13 is provided with a mounting cavity 134 for fixedly connecting with the output shaft of the driver 12, and the mounting cavity 134 penetrates the first shaft portion 131 from the end surface of the first shaft portion 131 and extends to the second shaft portion 132. The axis of the mounting cavity 134 is not coincident with the axis of the third shaft portion 133, so as to form an eccentric shaft structure, the output shaft of the driving member 12 is fixedly arranged in the mounting cavity 134, when the output shaft of the driving member 12 rotates, the driving member 12 drives the eccentric shaft 13 to rotate around the axis of the mounting cavity 134, and at this time, the third shaft portion 133 performs eccentric rotation movement because the axis of the mounting cavity 134 is not coincident with the axis of the third shaft portion 133. The center of the oscillating platform 4 is provided with a connecting hole, and the third shaft portion 133 is sleeved in the connecting hole to drive the oscillating platform 4 to perform eccentric rotation motion.

Specifically, the third shaft portion 133 of the eccentric shaft 13 is sleeved with the oscillating platform 4 to drive the oscillating platform 4 to perform eccentric rotation motion. In order to ensure the stability and smoothness of the rotation of the eccentric shaft 13, the third shaft 133 is sleeved with a second bearing 62. The upper end of the third shaft portion 133 is provided with a second bearing end cap that mates with the second bearing 62. It should be noted that the upper surface of the second bearing end cap needs to be located on the same plane as the upper surface of the oscillating platform 4, so as to be able to stably place the heating element 2. The second bearing end cap may be fixed to the third shaft portion 133 by a screw or bolt.

The first shaft portion 131 and the second shaft portion 132 of the eccentric shaft 13 each pass through the fixed plate 11 and are rotatable relative to the fixed plate 11 about the axis of the mounting cavity 134.

Specifically, a first annular portion 64 and a second annular portion 65 are further sleeved between the first bearing 61 and the second bearing 62 on the eccentric shaft 13. Wherein the upper end of the first bearing 61 abuts the lower surface of the first annular portion 64 and the lower end of the second bearing 62 abuts the upper surface of the second annular portion 65.

The first annular portion 64 and the second annular portion 65 enable the two bearings to be restrained, while also facilitating the mounting of the eccentric shaft 13.

The eccentric shaft 13 is mounted on the fixed plate 11 through the first annular portion 64 and the second annular portion 65. Specifically, an eccentric shaft mounting hole is formed in the fixing plate 11, and the cross section of the eccentric shaft mounting hole is in a T shape and comprises a large hole end and a small hole end. The first and second

annular portions

64, 65 are located within the bore end and are fixedly connected to the fixed plate 11 by bolts. A first bearing 61 is located in the small bore end.

The provision of the first bearing 61 and the second bearing 62 makes the rotation of the eccentric shaft 13 more stable and smooth.

Meanwhile, in order to fix the fixing plate 11 to the support frame of the scroll oscillating device, connectors (not shown) are provided at opposite sides of the fixing plate 11. The fixed plate 11 can be fixedly connected with the support frame through two connecting pieces, so that the vortex oscillation device can work normally.

Alternatively, lightening holes may be provided on the fixing plate 11.

When gathering DNA, place four sample loading boards 3 respectively on a sample loading board places platform 8, start driving piece 12, the eccentric rotary motion is started to the oscillation platform 4 to drive heating element 2 and also be eccentric rotary motion, vortex oscillation promptly, heating element 2 also heats the sample of gathering simultaneously, shakes magnetic bead or silicon pearl fast and looses, accomplishes the collection of DNA.

The foregoing embodiments are merely illustrative of the principles and features of this invention, which is not limited to the above-described embodiments, but rather is susceptible to various changes and modifications without departing from the spirit and scope of the utility model, which changes and modifications are within the scope of the utility model as claimed. The scope of the utility model is defined by the appended claims and equivalents thereof.

Claims

1. A vortex oscillation apparatus comprising:

an oscillating assembly (1) comprising an oscillating platform (4), said oscillating platform (4) being capable of eccentric rotary motion;

the sample loading plate placing platform comprises a plurality of sample loading plate placing platforms (8), the sample loading plate placing platforms (8) are arrayed on the oscillating platform (4), each sample loading plate placing platform (8) can place one sample loading plate (3), and each sample loading plate placing platform (8) is provided with a limiting block (43) for limiting the sample loading plates (3) on the sample loading plate placing platforms (8).

2. A vortex oscillation device according to claim 1, characterized in that a heating assembly (2) is arranged on each sample carrier plate placement stage (8), said heating assembly (2) being used to heat the sample carrier plate (3) on the sample carrier plate placement stage (8).

3. A scroll oscillation apparatus as claimed in claim 2, wherein the heating assembly (2) comprises a heating plate (21), the heating plate (21) being capable of generating heat.

4. A scroll oscillation device as claimed in claim 3, wherein a heat conductive plate is provided above the heating plate (21), and the heat of the heating plate (21) is transferred to the sample carrier plate (3) via the heat conductive plate.

5. A scroll oscillation apparatus as claimed in claim 3, wherein a heat insulating plate (23) is provided below the heating sheet (21).

6. A vortex oscillation device according to claim 1, characterized in that the stopper (43) comprises a first stopper portion (432) and a second stopper portion (433) arranged perpendicular to each other to limit the sample carrier plate (3).

7. A scroll oscillation device according to claim 6, wherein an elastic buffer block (431) is provided on each of the first stopper portion (432) and the second stopper portion (433), the elastic buffer block (431) being capable of elastically colliding with the sample carrier plate (3).

8. A scroll oscillation apparatus as claimed in claim 7, wherein said elastic damping block (431) includes a damping housing (4311) and an elastic damping piece (4312) which is caught in said damping housing (4311).

9. A scroll vibration device according to any one of claims 1 to 8, further comprising a fixed plate (11), wherein said vibration table (4) is provided on said fixed plate (11) and said vibration table (4) is capable of eccentric rotational movement with respect to said fixed plate (11).

10. A scroll oscillation apparatus as claimed in claim 9, wherein the oscillation assembly (1) further comprises:

a driver (12);

one end of the eccentric shaft (13) is fixedly connected with the output end of the driving piece (12), the other end of the eccentric shaft penetrates through the fixing plate (11) to be connected with the oscillating platform (4), and the eccentric shaft (13) can drive the oscillating platform (4) to do eccentric rotation motion.