CN212246984U - Vortex oscillation device - Google Patents
Vortex oscillation device Download PDFInfo
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- CN212246984U CN212246984U CN202020394098.4U CN202020394098U CN212246984U CN 212246984 U CN212246984 U CN 212246984U CN 202020394098 U CN202020394098 U CN 202020394098U CN 212246984 U CN212246984 U CN 212246984U
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Abstract
The utility model discloses a vortex oscillation device, it belongs to oscillation device technical field, and vortex oscillation device includes: an oscillating assembly comprising an oscillating platform capable of eccentric rotational movement; the heating assembly is arranged on the oscillating platform, the oscillating platform can drive the heating assembly to do eccentric rotation motion, a cell culture plate for placing a sample can be placed on the heating assembly, and the heating assembly can heat the cell culture plate. The utility model discloses can accomplish sample DNA's collection fast.
Description
Technical Field
The utility model relates to an oscillation device technical field especially relates to a vortex oscillation device.
Background
When extracting DNA by the magnetic bead method or the silica bead method, a vortex oscillator is generally used to perform vortex oscillation on a sample solution.
Taking magnetic bead method to extract DNA as an example, in the prior art, the collected sample is processed in the early stage and is cracked to obtain supernatant. The magnetic bead suspension was added to the supernatant and vortexed using a vortexer in preparation for extracting DNA from the liquid.
In the prior art, the speed of vortex oscillation for vibrating magnetic beads is slow, which affects the extraction efficiency of DNA.
Therefore, a vortex oscillation device is needed to solve the above problems.
SUMMERY OF THE UTILITY MODEL
An object of the utility model is to provide a vortex oscillation device to solve the technical problem that the extraction rate is slower when the vortex oscillation appearance that exists among the prior art draws DNA.
As the conception, the utility model adopts the technical proposal that:
a vortex oscillation apparatus comprising:
an oscillating assembly comprising an oscillating platform capable of eccentric rotational movement;
the heating assembly is arranged on the oscillating platform, the oscillating platform can drive the heating assembly to do eccentric rotation motion, a cell culture plate for placing a sample can be placed on the heating assembly, and the heating assembly can heat the cell culture plate.
Optionally, the heating assembly comprises a heating plate, the heating plate being capable of generating heat.
Optionally, the heating assembly further comprises a heat conducting plate, the heat conducting plate is stacked above the heating plate, and the heat of the heating plate is transferred to the cell culture plate through the heat conducting plate.
Optionally, the heating assembly further comprises a temperature controller to control the temperature of the heating assembly.
Optionally, the vortex oscillation device further includes a fixed plate, and the oscillation platform is disposed on the fixed plate and can make eccentric rotation motion relative to the fixed plate.
Optionally, a limiting column is arranged on the fixing plate, and a first cylindrical cavity matched with the limiting column is arranged on the lower surface of the oscillating platform.
Optionally, a buffering structure is disposed between the oscillating platform and the fixing plate, so that noise between the oscillating platform and the fixing plate can be reduced.
Optionally, the buffer structure comprises:
the convex column is arranged on the upper surface of the fixing plate;
the buffer ring is sleeved on the protruding column.
Optionally, the lower surface of the oscillation platform is provided with a second cylindrical cavity matched with the buffer structure, the oscillation platform is installed in the fixing plate, the buffer structure is located in the second cylindrical cavity, and the buffer ring can elastically collide with the inner wall of the second cylindrical cavity.
Optionally, the oscillating assembly further comprises:
a drive member;
and one end of the eccentric shaft is fixedly connected with the output end of the driving piece, the other end of the eccentric shaft penetrates through the fixing plate to be connected with the oscillating platform, and the eccentric shaft can drive the oscillating platform to do eccentric rotation motion.
The utility model provides a vortex oscillation device has following advantage:
when gathering DNA, gather the sample and place on the cell culture board, heat the cell culture board by heating element, and then make the temperature rise of gathering the sample, drive heating element by the oscillation platform and be eccentric rotary motion, and then drive the cell culture board and carry out the vortex oscillation. The temperature is increased, magnetic beads or silicon beads can be quickly scattered, and the extraction speed of DNA is increased.
Drawings
Fig. 1 is a schematic view of a vortex oscillation apparatus provided by an embodiment of the present invention in use;
fig. 2 is a schematic cross-sectional view of a part of the internal structure of a vortex oscillation device according to an embodiment of the present invention;
fig. 3 is a schematic structural diagram of the vortex oscillation device provided by the embodiment of the present invention after the limiting block is hidden;
fig. 4 is a schematic view of a viewing angle when the oscillating platform and the fixing plate are not assembled according to an embodiment of the present invention;
fig. 5 is a schematic view of another view angle of the oscillating platform and the fixing plate provided by the embodiment of the present invention when they are not assembled;
fig. 6 is a schematic structural view of a limiting post provided in an embodiment of the present invention;
fig. 7 is a schematic structural diagram of an elastic buffer block according to an embodiment of the present invention;
fig. 8 is a schematic structural view of an elastic buffer sheet according to an embodiment of the present invention;
fig. 9 is a schematic structural diagram of an eccentric shaft according to an embodiment of the present invention.
In the figure:
1. an oscillating assembly; 11. a fixing plate; 111. a limiting column; 1111. a first flange; 1112. a second flange; 12. a drive member; 13. an eccentric shaft; 131. a first shaft portion; 132. a second shaft portion; 133. a third shaft portion; 134. a mounting cavity;
2. a heating assembly; 21. a heating plate; 22. a heat conducting plate; 23. a temperature controller; 24. a heat insulation plate;
3. a cell culture plate;
4. an oscillating platform; 41. a first cylindrical cavity; 42. a second cylindrical cavity; 43. a limiting block; 431. an elastic buffer block; 4311. a buffer housing; 4312. an elastic buffer sheet; 43121. a first part; 43122. a second section; 43123. a third section;
5. a buffer structure; 51. a raised post; 52. a buffer ring;
61. a first bearing; 62. a second bearing; 63. a first bearing end cap; 64. a first annular portion; 65. a second annular portion;
7. a connecting member.
Detailed Description
In order to make the technical problem solved by the present invention, the technical solution adopted by the present invention and the technical effect achieved by the present invention clearer, the technical solution of the present invention will be further explained by combining the drawings and by means of the specific implementation manner. It is to be understood that the specific embodiments described herein are merely illustrative of the invention and are not limiting of the invention. It should be further noted that, for the convenience of description, only some but not all of the elements related to the present invention are shown in the drawings.
In the description of the present invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and simplification of description, but do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus, should not be construed as limiting the present invention. Furthermore, the terms "first," "second," and the like are used for descriptive purposes only and are not to be construed as indicating or implying relative importance. Wherein the terms "first position" and "second position" are two different positions.
In the description of the present invention, it is to be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "connected," and "connected" are to be construed broadly, e.g., as meaning either a fixed connection or a removable connection; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meaning of the above terms in the present invention can be understood in specific cases to those skilled in the art.
The embodiment provides a vortex oscillation device, can vibrate magnetic bead or silica bead fast, accomplish the extraction of sample DNA.
Referring to fig. 1 to 9, in the present embodiment, the vortex oscillation apparatus includes an oscillation assembly 1 and a heating assembly 2.
The oscillating assembly 1 comprises an oscillating platform 4, the oscillating platform 4 being capable of eccentric rotary motion. The heating assembly 2 is arranged on the oscillating platform 4. The oscillation platform 4 can drive the heating element 2 to do eccentric rotation motion, the cell culture plate 3 for placing the sample can be placed on the heating element 2, and the heating element 2 can heat the cell culture plate 3.
The cell culture plate 3 serves to carry the sample to be collected. Alternatively, in this embodiment, the cell culture plate 3 is a 96-well plate.
Specifically, in the present embodiment, the heating assembly 2 includes a heating sheet 21 and a heat conductive plate 22.
The heating sheet 21 is provided with a heating wire therein to generate heat. The heat conducting plate 22 is stacked above the heating sheet 21, and the heat of the heating sheet 21 is transferred to the cell culture plate 3 via the heat conducting plate 22. The heat conducting plate 22 can transfer heat more uniformly, which is beneficial to the rapid extraction of the sample DNA in the cell culture plate 3.
Optionally, the heating assembly 2 further comprises a thermostat 23 to control the temperature of the heating assembly 2. Of course, in other embodiments, the heating assembly 2 may be provided with a temperature sensor to monitor the temperature of the heating assembly 2 in real time.
Specifically, in the present embodiment, the thermostat 23 is electrically connected to the heating sheet 21. The temperature sensor may be disposed on the heating sheet 21 to monitor the temperature of the heating sheet 21 in real time. The temperature controller 23 is used to control the temperature of the heating sheet 21.
Of course, in other embodiments, the temperature sensor may also be disposed on the heat conducting plate 22 to monitor the temperature of the heat conducting plate 22 in real time, so as to detect the temperature of the cell culture plate more accurately.
Further, in order to prevent heat loss, the heating assembly in this embodiment further includes a heat insulation board 24. Specifically, the heat insulation plate 24 is disposed below the heating sheet 21 to prevent heat dissipation and ensure the heating effect of the heating assembly 2 on the cell culture plate 3.
Further, the vortex oscillation device further comprises a fixing plate 11, the oscillation platform 4 is arranged on the fixing plate 11, and the oscillation platform 4 can do eccentric rotation motion relative to the fixing plate 11. In using the vortex oscillation device, the fixing plate 11 may be fixed to the frame to provide a support platform for the vortex oscillation device. In the working process, the oscillating platform 4 can do eccentric rotation motion relative to the fixed plate 11, so that the vortex oscillation of the cell culture plate 3 is realized.
Specifically, referring to fig. 4 and 5, in the present embodiment, the fixing plate 11 is provided with a limiting column 111, the lower surface of the oscillating platform 4 is provided with a first cylindrical cavity 41 matched with the limiting column 111, and the limiting column 111 can rock in the first cylindrical cavity 41. When the oscillating platform 4 performs eccentric rotation, the limit column 111 can limit the oscillating platform 4, so as to prevent the oscillating platform 4 from exceeding a limit position due to inertia.
Further, referring to fig. 6, in this embodiment, two ends of the limiting column 111 are respectively provided with a first flange 1111 and a second flange 1112, the fixing plate 11 is provided with a limiting column mounting cavity, a lower half portion of the limiting column 111 is located in the limiting column mounting cavity, the second flange 1112 is clamped in a second flange clamping groove in the limiting column mounting cavity, an upper half portion of the limiting column 111 is located in the first cylindrical cavity 41, and a diameter of the first cylindrical cavity 41 is greater than a diameter of the second flange 1112, so as to ensure that the limiting column 111 can rock in the first cylindrical cavity 41. Meanwhile, the arrangement of the second flange 1112 enables the upper end of the limiting column 111 to be more stably abutted to the oscillating platform 4, so that the moving stability of the oscillating platform 4 is ensured.
Preferably, two limiting columns 111 are respectively arranged at four corners of the fixing plate 11. When the oscillating platform 4 oscillates at a high speed, the plurality of stopper posts 111 located at the four corners can restrict the respective orientations of the oscillating platform 4.
Further, in order to reduce noise between the oscillation platform 4 and the fixed plate 11, a buffer structure 5 is provided between the oscillation platform 4 and the fixed plate 11.
Referring to fig. 4, the buffer structure 5 includes a protrusion post 51 and a buffer ring 52. The protruding column 51 is disposed on the upper surface of the fixing plate 11, and the buffer ring 52 is sleeved on the protruding column 51.
Specifically, the cushion ring 52 is made of a flexible cushion material, such as silicone or rubber.
Referring to fig. 5, the lower surface of the oscillating platform 4 is provided with a second cylindrical cavity 42 matched with the buffer structure 5, and when the oscillating platform 4 is installed on the fixing plate 11, the buffer structure 5 is located in the second cylindrical cavity 42 and the buffer ring 52 can elastically collide with the inner wall of the second cylindrical cavity 42, so that the collision force is reduced, and the noise is reduced.
Further, in order to prevent that heating element 2 and cell culture plate 3 break away from shaking platform 4 among the oscillation process, in this embodiment, four bights of shaking platform 4 respectively are provided with a stopper 43, and each stopper 43 all with heating element 2 and cell culture plate 3's lateral wall butt, four stoppers 43 block heating element 2 and cell culture plate 3 for heating element 2 and cell culture plate 3 are fixed with shaking platform 4's relative position, further guarantee the oscillation effect.
Specifically, the limiting block 43 is right-angled and includes a first limiting portion and a second limiting portion that are perpendicular to each other. In order to prevent the cell culture plate 3 from being damaged by too large collision force with the limiting block 43 in the oscillation process, the limiting block 43 is further provided with an elastic buffer block 431 to buffer the collision between the cell culture plate 3 and the limiting block 43. The elastic buffer block 431 is located inside the stopper 43 and buffers the collision between the cell culture plate 3 and the stopper 43 during the oscillation process.
Referring to fig. 7 and 8, in particular, in the present embodiment, the elastic buffer block 431 includes a buffer housing 4311 and an elastic buffer sheet 4312 clamped inside the buffer housing 4311.
Specifically, the elastic buffer sheet 4312 is made of spring steel, and includes a first portion 43121, a second portion 43122 and a third portion 43123 connected in sequence, where the first portion 43121 and the third portion 43123 are disposed at two opposite ends of the second portion 43122 and both form an obtuse angle with the second portion 43122, and the first portion 43121 and the third portion 43123 extend toward a direction close to the cell culture plate 3. The ends of the first portion 43121 and the third portion 43123 far from the second portion 43122 are provided with hooks for engaging with the buffer housing 4311.
The second portion 43122 is provided with a connection hole through which a bolt or a screw passes to connect with the stopper 43.
Preferably, in this embodiment, the first position-limiting part and the second position-limiting part are respectively provided with an elastic buffer block 431, which can limit two adjacent sides of the cell culture plate 3.
Further, the oscillating assembly 1 also comprises a driver 12 and an eccentric shaft 13: one end of the eccentric shaft 13 is fixedly connected with the output end of the driving part 12, the other end of the eccentric shaft passes through the fixing plate 11 and is fixedly connected with the oscillating platform 4, and the eccentric shaft 13 can drive the oscillating platform 4 to do eccentric rotation motion.
Optionally. The driving member 12 is a servo motor, and the servo motor can ensure the oscillation frequency and the stability of oscillation.
Specifically, referring to fig. 2 and 9, in order to enable the eccentric shaft 13 to drive the oscillating platform 11 to perform circumferential shaking when rotating, the eccentric shaft 13 includes a first shaft portion 131, a second shaft portion 132 and a third shaft portion 133, and the axes of the first shaft portion 131, the second shaft portion 132 and the third shaft portion 133 are not overlapped.
The eccentric shaft 13 is provided with a mounting cavity 134 for fixedly connecting with the output shaft of the driver 12, and the mounting cavity 134 penetrates the first shaft portion 131 from the end surface of the first shaft portion 131 and extends to the second shaft portion 132. The axis of the mounting cavity 134 is not coincident with the axis of the third shaft portion 133, so as to form an eccentric shaft structure, the output shaft of the driving member 12 is fixedly arranged in the mounting cavity 134, when the output shaft of the driving member 12 rotates, the driving member 12 drives the eccentric shaft 13 to rotate around the axis of the mounting cavity 134, and at this time, the third shaft portion 133 performs eccentric rotation movement because the axis of the mounting cavity 134 is not coincident with the axis of the third shaft portion 133. The center of the oscillating platform 4 is provided with a connecting hole, and the third shaft portion 133 is sleeved in the connecting hole to drive the oscillating platform 4 to perform eccentric rotation motion.
Specifically, the third shaft portion 133 of the eccentric shaft 13 is sleeved with the oscillating platform 4 to drive the oscillating platform 4 to perform eccentric rotation motion. In order to ensure the stability and smoothness of the rotation of the eccentric shaft 13, the third shaft 133 is sleeved with a second bearing 62. The upper end of the third shaft portion 133 is provided with a second bearing end cap that mates with the second bearing 62. It should be noted that the upper surface of the second bearing end cap needs to be located on the same plane as the upper surface of the oscillating platform 4, so as to be able to stably place the heating element 2. The second bearing end cap may be fixed to the third shaft portion 133 by a screw or bolt.
The first shaft portion 131 and the second shaft portion 132 of the eccentric shaft 13 each pass through the fixed plate 11 and are rotatable relative to the fixed plate 11 about the axis of the mounting cavity 134.
Specifically, a first annular portion 64 and a second annular portion 65 are further sleeved between the first bearing 61 and the second bearing 62 on the eccentric shaft 13. Wherein the upper end of the first bearing 61 abuts the lower surface of the first annular portion 64 and the lower end of the second bearing 62 abuts the upper surface of the second annular portion 65.
The first annular portion 64 and the second annular portion 65 enable the two bearings to be restrained, while also facilitating the mounting of the eccentric shaft 13.
The eccentric shaft 13 is mounted on the fixed plate 11 through the first annular portion 64 and the second annular portion 65. Specifically, an eccentric shaft mounting hole is formed in the fixing plate 11, and the cross section of the eccentric shaft mounting hole is in a T shape and comprises a large hole end and a small hole end. The first and second annular portions 64, 65 are located within the bore end and are fixedly connected to the fixed plate 11 by bolts. A first bearing 61 is located in the small bore end.
The provision of the first bearing 61 and the second bearing 62 makes the rotation of the eccentric shaft 13 more stable and smooth.
Meanwhile, in order to fix the fixing plate 11 to the supporting frame of the vortex oscillation device, the two opposite sides of the fixing plate 11 are provided with connecting pieces 7. The fixed plate 11 can be fixedly connected with the support frame through the two connecting pieces 7, and the vortex oscillation device can work normally.
Alternatively, lightening holes may be provided on the fixing plate 11.
When gathering DNA, start driving piece 12, the eccentric rotary motion is started to the vibration platform 4 to drive heating element 2 and also be eccentric rotary motion, vortex oscillation promptly, heating element 2 also heats gathering the sample simultaneously, and the collection of DNA is accomplished to quick vibration magnetic bead or silicon pearl.
The above embodiments have been described only the basic principles and features of the present invention, and the present invention is not limited by the above embodiments, and is not departing from the spirit and scope of the present invention. The scope of the invention is defined by the appended claims and equivalents thereof.
Claims (10)
1. A vortex oscillation apparatus comprising:
an oscillating assembly (1) comprising an oscillating platform (4), said oscillating platform (4) being capable of eccentric rotary motion;
heating element (2), locate on vibration platform (4), vibration platform (4) can drive heating element (2) are eccentric rotary motion, and cell culture board (3) for placing the sample can place in on heating element (2), heating element (2) can be right cell culture board (3) heating.
2. A scroll oscillation apparatus as claimed in claim 1, wherein the heating assembly (2) comprises a heating plate (21), the heating plate (21) being capable of generating heat.
3. A scroll-type oscillation device as claimed in claim 2, wherein the heating assembly (2) further comprises a heat-conducting plate (22), the heat-conducting plate (22) being superposed above the heating plate (21), the heat of the heating plate (21) being transferred to the cell culture plate (3) via the heat-conducting plate (22).
4. A scroll oscillator device according to claim 1, wherein the heating assembly (2) further comprises a thermostat (23) to control the temperature of the heating assembly (2).
5. The vortex oscillation apparatus of claim 1 further comprising a fixed plate (11), wherein the oscillation platform (4) is disposed on the fixed plate (11) and the oscillation platform (4) is capable of eccentric rotational movement relative to the fixed plate (11).
6. A scroll oscillation device as claimed in claim 5, wherein the fixing plate (11) is provided with a stopper post (111), and the lower surface of the oscillation platform (4) is provided with a first cylindrical cavity (41) engaged with the stopper post (111).
7. A scroll oscillation device according to claim 5, wherein a buffering structure (5) is provided between the oscillation platform (4) and the fixed plate (11) to reduce noise between the oscillation platform (4) and the fixed plate (11).
8. A scroll oscillation device according to claim 7, wherein the buffering structure (5) comprises:
the protruding columns (51) are arranged on the upper surface of the fixing plate (11);
and the buffer ring (52) is sleeved on the convex column (51).
9. A scroll oscillating device according to claim 8, wherein the lower surface of the oscillating platform (4) is provided with a second cylindrical cavity (42) cooperating with the buffer structure (5), the buffer structure (5) being located in the second cylindrical cavity (42) and the buffer ring (52) being capable of elastically colliding with the inner wall of the second cylindrical cavity (42) when the oscillating platform (4) is mounted to the fixed plate (11).
10. A scroll oscillation apparatus as claimed in claim 5, wherein the oscillation assembly (1) further comprises:
a driver (12);
one end of the eccentric shaft (13) is fixedly connected with the output end of the driving piece (12), the other end of the eccentric shaft penetrates through the fixing plate (11) to be connected with the oscillating platform (4), and the eccentric shaft (13) can drive the oscillating platform (4) to do eccentric rotation motion.
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CN202020394098.4U CN212246984U (en) | 2020-03-25 | 2020-03-25 | Vortex oscillation device |
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CN202020394098.4U CN212246984U (en) | 2020-03-25 | 2020-03-25 | Vortex oscillation device |
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Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN111269805A (en) * | 2020-03-25 | 2020-06-12 | 广州高盛生物科技股份有限公司 | Vortex oscillation device |
CN114015572A (en) * | 2022-01-04 | 2022-02-08 | 浙江金仪盛世生物工程有限公司 | Oscillator movement and oscillator |
-
2020
- 2020-03-25 CN CN202020394098.4U patent/CN212246984U/en active Active
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN111269805A (en) * | 2020-03-25 | 2020-06-12 | 广州高盛生物科技股份有限公司 | Vortex oscillation device |
CN114015572A (en) * | 2022-01-04 | 2022-02-08 | 浙江金仪盛世生物工程有限公司 | Oscillator movement and oscillator |
CN114015572B (en) * | 2022-01-04 | 2022-04-26 | 浙江金仪盛世生物工程有限公司 | Oscillator movement and oscillator |
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