CN216236959U

CN216236959U - Multi-stage cooperative algae culture reactor

Info

Publication number: CN216236959U
Application number: CN202122767143.3U
Authority: CN
Inventors: 易启甲; 张成武; 关辉
Original assignee: Shenzhen Yuanqi Biotechnology Co ltd
Current assignee: Shenzhen Yuanqi Biotechnology Co ltd
Priority date: 2021-11-12
Filing date: 2021-11-12
Publication date: 2022-04-08
Anticipated expiration: 2031-11-12

Abstract

The utility model relates to a multi-stage collaborative algae culture reactor, which comprises a reaction tank body, wherein the upper part of the reaction tank body is provided with a CIP cleaner and a liquid inlet, the middle part of the reaction tank body is provided with a plurality of LED light supplement lamps and sensors, the middle lower part of the reaction tank body is provided with a condensing coil pipe, and the bottom of the reaction tank body is provided with an aeration pipe; the interior of the reaction tank body comprises a plurality of subareas, each subarea is divided into a red-stage culture area and a green-stage culture area by a retractable light screen, the interior of a cylindrical area formed by the unfolded light screens is the red-stage culture area, and the exterior of the light screens is the green-stage culture area; a plurality of circles of LED light supplement lamps are arranged in the red stage cultivation area, and a plurality of circles of LED light supplement lamps are also arranged in the green stage cultivation area.

Description

Multi-stage cooperative algae culture reactor

Technical Field

The utility model belongs to the technical field of algae cultivation equipment, and particularly relates to a multi-stage collaborative algae cultivation reactor.

Background

Algae are eukaryon or prokaryote in the protist kingdom, are mostly aquatic, have no vascular bundle, and can perform photosynthesis. The size of the body is mostly below 100 microns, and a few algae have larger bodies and are similar to plants. Algae produce, reproduce and produce metabolites through autotrophy, are the main species in the nature, and as the living standard of people is improved, the attention to food health is increased, and algae such as chlorella and haematococcus pluvialis which can accumulate metabolites with nutritional values through self metabolism are more and more popular with consumers.

Haematococcus pluvialis is a unicellular green alga, and the Haematococcus pluvialis in the growth and proliferation stage is green cell; when the environmental conditions change to be unfavorable for the growth, such as nitrogen deficiency, phosphorus deficiency or strong light energy irradiation, the haematococcus pluvialis cells grow slowly and lose the motor flagella to form a glue colony or a chlamydospore, and simultaneously, a large amount of astaxanthin is accumulated in the cells to rapidly change the color of the haematococcus pluvialis cells to deep red.

In China, the culture of haematococcus pluvialis has already been primarily successful in the laboratory culture stage, and the industrial large-scale culture is being explored. However, the productivity of the industrial culture of haematococcus pluvialis is still low internationally. On the one hand, the reason is that the haematococcus pluvialis needs higher culture environment requirements and is sensitive to the change of the culture environment; on the other hand, the different production stages of haematococcus pluvialis have different requirements on culture environments, so that the current culture scheme is that algae cells cultured in the previous stage are transferred to another container for culture in the next stage, partial cells are dead in the transfer process, and the dead algae cells release metabolites such as toxins and the like in a new culture environment to pollute other algae cells and influence the overall astaxanthin yield.

The traditional haematococcus pluvialis culture reactor can only singly culture algae cells in a green stage (green stage) or algae cells in a red stage (red stage), and generally comprises two culture modes, wherein one mode is that the green stage culture is finished in one reactor, and then the algae cells are transferred to the other reactor for red stage culture; the other is to carry out green stage cultivation first and then red stage cultivation in the same reactor. The two modes are both used for providing a culture condition in the same reactor and the same time period, so that the culture operation is convenient, and the growth state of the algae cells is unified. However, the existing mode either occupies more reactors or has a long whole culture period, and continuous production cannot be realized.

SUMMERY OF THE UTILITY MODEL

Aiming at the problems, the utility model provides a multi-stage collaborative algae culture reactor, which comprises a reaction tank body, wherein the upper part of the reaction tank body is provided with a CIP cleaner and a liquid inlet, the middle part of the reaction tank body is provided with a plurality of LED light supplement lamps and sensors, the middle lower part of the reaction tank body is provided with a condensing coil pipe, and the bottom of the reaction tank body is provided with an aeration pipe;

the interior of the reaction tank body comprises a plurality of subareas, each subarea is divided into a red-stage culture area and a green-stage culture area by a retractable light screen, the interior of a cylindrical area formed by the unfolded light screens is the red-stage culture area, and the exterior of the light screens is the green-stage culture area; a plurality of circles of LED light supplement lamps are arranged in the red stage cultivation area, and a plurality of circles of LED light supplement lamps are also arranged in the green stage cultivation area.

Optionally, the liquid inlet comprises a water inlet, an algae seed inlet, a nutrient solution inlet and a steam inlet, and the water inlet is connected with the pure water tank and supplies pure water to the reactor; the algae seed inlet is connected with an algae seed box and supplies algae seeds for the reactor when the reactor is just started; the nutrient solution inlet is connected with a nutrient solution box and supplies nutrient solution for the reactor; the steam inlet is connected with a steam generator, and when a batch of algae is cultured and algae liquid is discharged, steam is used for disinfecting the reactor.

Optionally, the sensors include a temperature sensor, a density sensor, a pH sensor and a liquid level sensor, and the aeration pipes include a carbon dioxide aeration pipe and a compressed air aeration pipe.

Further optionally, the carbon dioxide aeration pipe is sequentially connected with a carbon dioxide flow meter, a carbon dioxide air pump and a carbon dioxide storage tank through an air pipe, and the compressed air aeration pipe is sequentially connected with a compressed air flow meter, a compressed air pump and a compressed air storage tank through an air pipe.

Optionally, the CIP cleaner is connected in parallel with the pure water tank and the disinfectant tank through a pipeline, and is used for spraying disinfectant and cleaning water into the reactor; and two ends of the condensing coil are respectively connected with a water cooler through pipelines.

Optionally, a harvesting outlet is arranged at the bottom of the reaction tank body, and the reaction tank body is sequentially connected with a liquid pump, a centrifuge and a product storage tank through pipelines; the middle lower part of the reaction tank body is provided with a sampling port.

Optionally, the culture reactor is in the shape of a vertical cylinder, and the interior of the culture reactor comprises a plurality of partitions in the shape of vertical cylinders.

Optionally, the arrangement form of the subareas in the reaction tank body of the culture reactor is as follows: the plurality of the subareas are arranged into a plurality of circles from the inner wall of the reaction tank body to the center of the reaction tank body (namely from outside to inside), the subarea of each circle is tangent to the subareas of the adjacent circles, and the subareas forming each circle are tangent to each other; and an irregularly-shaped vacant area is formed between the partitions of the two adjacent circles.

Optionally, one of the subareas comprises the red-stage cultivation area, a light shielding plate and a green-stage cultivation area from inside to outside, the red-stage cultivation area is provided with a plurality of lamp rings from inside to outside, each lamp ring is formed by arranging a plurality of LED light supplement lamps at the same interval, the intervals between adjacent lamp rings are equal, and algal cells or spores in the red stage are distributed among LED light supplement lamp gaps of the red-stage cultivation area to metabolize to generate astaxanthin;

the green stage culture area is outwards set up a plurality of rings of lamp rings by the light screen, and every lamp ring is formed by the same distance arrangement of a plurality of LED light filling lamp interval, and the interval of adjacent lamp ring is equal, and the algae cell or the spore that are in the green stage distribute and grow and breed between the LED light filling lamp clearance in green stage culture area, when cell density and/or cell number reach the default, under the aeration, get into red stage culture area and accumulate astaxanthin.

Optionally, the light screen includes that a plurality of root are arranged into bracing piece and the shading of circle and are rolled up the curtain, the vertical setting of bracing piece, the inside spool that is equipped with of bracing piece, roll up on the spool and roll up the shading and roll up the curtain, the side of bracing piece is equipped with a roll curtain export, the height that highly is not less than the shading of rolling up the curtain export is rolled up the top and the bottom that the shading is rolled up the curtain and is close to one side of rolling up the curtain export and is equipped with the haulage rope respectively, the top and the bottom of the spool of adjacent bracing piece are connected respectively to the other end of haulage rope.

Optionally, the nutrient solution inlet is connected in parallel with a plurality of nutrient solution branch pipes, and each nutrient solution branch pipe corresponds to the upper part of the red-stage culture area of each partition.

Optionally, the algae seed inlet is connected in parallel with a plurality of algae seed branch pipes, and each algae seed branch pipe corresponds to the upper part of the green stage culture area of each subarea.

Optionally, the compressed air aeration pipe is divided into a strong aeration area and a weak aeration area at a part corresponding to each partition, and the strong aeration area corresponds to a red-stage culture area and provides more oxygen for heterotrophic algae cells; the weak aeration area corresponds to the green-stage culture area, the aeration holes of the weak aeration area are divided into two parts, one part of the aeration holes are inclined towards the red-stage culture area, the inclination angle is 20-60 degrees, and when algae cells are about to enter the red culture stage, the algae cells which grow mature are driven to move to the red-stage culture area to accumulate astaxanthin; the other part of aeration holes are in the common vertical upward direction and provide oxygen for the algae cells in the green stage culture area; the air holes of the two parts are uniformly distributed in a staggered way.

Optionally, each vacant zone is provided with a vertical mixing aeration pipe, and the height of the mixing aeration pipe is not lower than that of the subarea; the bottom of the mixing aeration pipe is communicated with the carbon dioxide aeration pipe and the compressed air aeration pipe, so that both carbon dioxide and compressed air can be introduced into the mixing aeration pipe; the side surfaces of the mixing aeration pipes facing the subareas are provided with vertical aeration holes.

Drawings

FIG. 1 is a schematic diagram of a culture reactor;

FIG. 2 is a schematic view of the arrangement of the zones in the reaction tank;

FIG. 3 is a schematic illustration of a partition;

fig. 4 is a schematic structural diagram of the light shielding plate.

In the attached drawing, 1-a reaction tank body, 101-a water inlet, 102-an algae seed inlet, 103-a nutrient solution inlet, 104-a steam inlet, 105-a temperature sensor, 106-a density sensor, 107-a pH sensor, 108-a liquid level sensor, 109-a carbon dioxide aeration pipe, 110-a compressed air aeration pipe, 111-a harvesting outlet, 112-a sampling port, 113-a sight glass, a 2-CIP cleaner, a 3-LED light supplement lamp, 301-a bracket, 4-a condensing coil, 5-a light screen, 501-a support rod, 502-a light-shielding rolling curtain, 503-a rolling shaft, 504-a rolling curtain outlet, 505-a traction rope, a 6-red stage culture area, a 7-green stage culture area, an 8-PLC (programmable logic controller) and 9-a subarea, 10-vacant area, 11-lamp ring and 12-mixed aeration pipe.

Detailed Description

The multi-stage collaborative algae culture reactor provided by the embodiment comprises a reaction tank body 1, wherein a CIP cleaner 2 and a liquid inlet are arranged at the upper part of the reaction tank body 1, a plurality of LED light supplement lamps 3 and sensors are arranged at the middle part of the reaction tank body, a condensing coil 4 is arranged at the middle lower part of the reaction tank body, and an aeration pipe is arranged at the bottom of the reaction tank body;

the inside of the reaction tank body 1 comprises a plurality of subareas 9, each subarea 9 is divided into a red-stage culture area 6 and a green-stage culture area 7 by a retractable light screen 5, the inside of a cylindrical area formed by the unfolded light screen 5 is the red-stage culture area 6, and the outside of the light screen 5 is the green-stage culture area 7; a plurality of circles of LED light supplement lamps 3 are arranged in the red-stage cultivation area 6, and a plurality of circles of LED light supplement lamps 3 are also arranged in the green-stage cultivation area 7.

Optionally, the liquid inlet includes a water inlet 101, an algae seed inlet 102, a nutrient solution inlet 103 and a steam inlet 104, and the water inlet 101 is connected to the pure water tank to supply pure water to the reactor; the algae seed inlet 102 is connected with an algae seed box and supplies algae seeds for the reactor when the reactor is just started; the nutrient solution inlet 103 is connected with a nutrient solution box and supplies nutrient solution for the reactor; the steam inlet 104 is connected to a steam generator, and when a batch of algae is completed and algae liquid is discharged, steam is used to sterilize the reactor.

Optionally, the sensors include a temperature sensor 105, a density sensor 106, a pH sensor 107 and a liquid level sensor 108, the aeration pipes include a carbon dioxide aeration pipe 109 and a compressed air aeration pipe 110, the LED light supplement lamp 3 is fixed by a bracket 301, and the LED light supplement lamp 3 is a common LED light supplement lamp 3 in the market.

Optionally, the CIP cleaner 2, the LED fill light 3, the temperature sensor 105, the density sensor 106, the pH sensor 107 and the liquid level sensor 108 are in communication connection with the PLC controller 8.

Optionally, the CIP cleaner 2 is connected in parallel with the pure water tank and the disinfectant tank through a pipeline, and is used for spraying disinfectant and cleaning water into the reactor; the two ends of the condensing coil 4 are respectively connected with a water chiller through pipelines, and the cold energy brought by the condensing coil 4 can adjust the temperature of the algae liquid rising due to the heat generated by the LED light supplement lamp 3 and the metabolism of algae cells.

Further optionally, the carbon dioxide aeration pipe 109 is sequentially connected to the carbon dioxide flow meter, the carbon dioxide air pump and the carbon dioxide storage tank through an air pipe, and the compressed air aeration pipe 110 is sequentially connected to the compressed air flow meter, the compressed air pump and the compressed air storage tank through an air pipe.

Further optionally, a plurality of peeping mirrors 113 are arranged on the side surface of the reaction tank body 1, so that the culture condition in the reaction tank body 1 can be observed conveniently; the bottom of the reaction tank body 1 is provided with a harvesting outlet 111, and is sequentially connected with a liquid pump, a centrifugal machine and a product storage tank through pipelines, the pipelines can also be connected with a discharge pipeline in parallel, and the discharge pipeline is connected with a waste recovery device or a sewer pipeline and is used for discharging the waste water body in the collected reaction tank body 1; the middle lower part of the reaction tank body 1 is provided with a sampling port 112 for sampling and detecting the growth condition of algae cells and the condition of algae liquid in real time.

Optionally, the PLC controller 8 controls the culture reactor through an internal preset control program, so as to realize full-automatic culture.

Optionally, the culture reactor is in the shape of a vertical cylinder, and the interior of the culture reactor comprises a plurality of partitions 9 in the shape of vertical cylinders.

Optionally, the arrangement form of the partition 9 in the reaction tank body 1 of the culture reactor is as follows: the plurality of the subareas 9 are arranged into a plurality of circles from the inner wall of the reaction tank body 1 to the center (namely from outside to inside) of the reaction tank body 1, the subarea 9 of each circle is tangent with the subareas 9 of the adjacent circles, and the subareas 9 forming each circle are tangent with each other; the partitions 9 of two adjacent circles form an irregularly shaped vacant area 10.

In one embodiment of the utility model, a first subarea 9 circle is arranged along the inner wall of the reaction tank body 1, the subareas 9 forming the first subarea 9 circle are tangent to the inner wall of the reaction tank body 1, and the adjacent subareas 9 are tangent to each other; 9 circles of second partitions are arranged in the 9 circles of first partitions, the partitions 9 forming the 9 circles of second partitions are tangent to the partitions 9 of the 9 circles of first partitions, and the adjacent partitions 9 are tangent to each other; a third partition 9 ring is arranged in the second partition 9 ring, the partitions 9 forming the third partition 9 ring are all tangent to the partitions 9 of the second partition 9 ring, and the adjacent partitions 9 are tangent to each other; by analogy, it is possible to accommodate only one or two compartments 9, arranged up to the centre of the cross-section of the reaction vessel 1.

The reaction tank body 1 is internally divided into a plurality of cylindrical subareas 9, each subarea 9 has the same structure, and algae cell culture in a red stage and a green stage can be simultaneously carried out in each subarea 9, so that areas except the vacant area 10 in the reaction tank body 1 can obtain culture conditions and environments with balanced conditions, the problem that the culture conditions are unbalanced or light cannot irradiate certain areas in the traditional reactor is avoided, and the problem that the algae cell density in a certain area is too high to shade the light or the nutrient content is insufficient is also avoided. Technicians can independently adjust the culture conditions of the subareas 9 according to the conditions of the subareas 9 to prevent the algae cells in other areas from being influenced, so that the regulation and control inside the whole reaction tank body 1 are more accurate, and the improvement of the culture efficiency of algae and the accumulation of astaxanthin are facilitated.

Optionally, one of the sub-areas 9 includes, from inside to outside, the red-stage cultivation area 6, the light shielding plate 5 and the green-stage cultivation area 7, the red-stage cultivation area 6 is provided with a plurality of lamp rings 11 from inside to outside, each lamp ring 11 is formed by arranging a plurality of LED light supplement lamps 3 at equal intervals, the intervals between adjacent lamp rings 11 are equal, and algal cells or spores in the red stage are distributed among the gaps of the LED light supplement lamps 3 in the red-stage cultivation area 6 to be metabolized to generate astaxanthin;

the green phase cultivation area 7 is outwards set up a plurality of rings of lamp rings 11 by light screen 5, and every lamp ring 11 is formed by arranging the same distance at a plurality of LED light filling lamps 3 interval, and the interval of adjacent lamp rings 11 is equal, and the algae cell or spore that are in the green phase distribute and grow and breed between the 3 clearances of LED light filling lamp in green phase cultivation area 7, when cell density and/or cell quantity reach the default, under the aeration effect, get into 6 accumulations astaxanthin in red phase cultivation area.

The distance between the adjacent lamp rings 11 of the green-stage cultivation area 7 and the red-stage cultivation area 6 and the distance between the adjacent LED light supplement lamps 3 can be adjusted according to the actual cultivation situation. The centers of the lamp rings 11 in the two regions are the same, that is, the lamp rings 11 are arranged in a concentric circle manner. The area inside the lamp ring 11 and the area outside the lamp ring can contain and culture algae cells, for example, the distance between the outermost lamp ring 11 of the green-stage culture area 7 and the adjacent lamp ring 11 at the inner side is h meters, the outward irradiation range of the outermost lamp ring 11 is h/meter, the inward irradiation range is h/meter, the outward irradiation range of the adjacent lamp ring 11 at the inner side is h/meter, namely, the algae cells between the two lamp rings 11 can all receive illumination, and the light energy is not overlapped and wasted; the boundary of one subarea 9 is the position of the outermost circle of the lamp ring 11 of the green-stage cultivation area 7 extending outwards for h/meter, namely the boundary of the subarea 9 is not physically separated by equipment, but is defined according to the action range of the outermost circle of the lamp ring 11 of the green-stage cultivation area 7.

The interior of the subarea 9 is divided into two areas, the algae cells in the green stage swim independently or are driven to the red-stage culture area 6 in the subarea 9 by aeration, the two areas can implement different culture conditions at the same time, the algae cells in the two stages are cultured at the same time, the culture efficiency is improved, the occupied land is saved, and the production period is shortened.

Optionally, the light shielding plate 5 includes a plurality of supporting rods 501 arranged in a circle and a light shielding roller shutter 502, the supporting rods 501 are vertically arranged, a roller 503 is arranged inside the supporting rods 501, the light shielding roller shutter 502 is wound on the roller 503, a roller shutter outlet 504 is arranged on a side of the supporting rods 501, the height of the roller shutter outlet 504 is not less than the height of the light shielding roller shutter 502, traction ropes 505 are respectively arranged at the top and the bottom of one side of the light shielding roller shutter 502 close to the roller shutter outlet 504, and the other ends of the traction ropes 505 are respectively connected to the top and the bottom of the roller shutter 503 of the adjacent supporting rods 501.

In a specific embodiment of the utility model, a plurality of support rods 501 are arranged in a circle at a certain distance from the outermost lamp ring 11 of the red-stage cultivation area 6 inside the support rods, and the circle of each support rod 501 is the same as the circle center of the lamp ring 11; the rolling screen outlet 504 of one support bar 501 faces the opposite side of the rolling screen outlet 504 of the adjacent support bar 501, for example, the side of the support bar 501 having the rolling screen outlet 504 is defined as the front side, the opposite side of the rolling screen outlet 504 is defined as the back side, the front side of one support bar 501 faces the back side of the adjacent support bar 501 in the clockwise direction or the counterclockwise direction, and so on. The reel 503 is fixed on the bottom plate of the reaction tank body 1, and penetrates through the bottom plate to be connected with the corresponding driving motor, the reel 503 penetrates through the bottom plate to be provided with a sealing device, a plurality of driving motors are arranged below the reaction tank body 1, one driving motor is connected with the reel 503 of one support rod 501, the driving reel 503 rotates, the shading roller shutter 502 is unreeled, and meanwhile the traction rope 505 of the adjacent support rod 501 is reeled, so that the shading roller shutter 502 of the adjacent support rod 501 is pulled, the outer side edge of the shading roller shutter 502 reaches the back surface of the adjacent support rod 501, and the shading of the area between the two adjacent support rods 501 is completed.

The algae cells in the red stage need strong illumination to accumulate astaxanthin, so the illumination of the LED light supplement lamp 3 of the red stage culture area 6 is stronger than that of the green stage culture area 7, and after the shading roller shutter 502 is unreeled, the strong light of the red stage culture area 6 can be shielded; after the shading roller shutter 502 is rolled, the algae cells in the green stage culture area 7 can be allowed to enter the red stage culture area 6.

Optionally, the nutrient solution inlet 103 is connected in parallel with a plurality of nutrient solution branch pipes, and each nutrient solution branch pipe corresponds to the upper part of the red-stage culture area 6 of each partition 9 and is used for providing nutrients required by growth, reproduction and metabolism of algae cells to each partition 9. As the algae cells in the red stage are mainly heterotrophic and need more nutrients, the nutrient solution is divided into tubes and is positioned in the red stage culture area 6, the algae cells in the red stage are supplied firstly, the nutrient solution diffuses outwards into the green stage culture area 7, the concentration of the nutrients is reduced to some extent, and the nutrient solution is just suitable for the algae cells in the green stage to take nutrition.

Optionally, the algae seed inlet 102 is connected in parallel with a plurality of algae seed branch pipes, and each algae seed branch pipe corresponds to the upper part of the green stage cultivation area 7 of each partition 9 and is used for providing raw algae seeds for each partition 9. Because the green-stage culture area 7 surrounds the periphery of the red-stage culture area 6 in a circular ring shape, the algae seed branch pipes are preferably arranged at the part of the green-stage culture area 7 far away from the red-stage culture area 6, so that the algae seeds are driven by aeration to move towards the red-stage culture area 6 in the growth and propagation processes.

Optionally, the compressed air aeration pipe 110 is divided into a strong aeration area and a weak aeration area at the part corresponding to each partition 9, and the strong aeration area corresponds to the red stage culture area 6 and provides more oxygen for the heterotrophic algae cells; the weak aeration area corresponds to the green-stage culture area 7, and aeration holes of the weak aeration area are divided into two parts, wherein one part of the aeration holes are inclined towards the direction of the red-stage culture area 6, the inclination angle is 20-60 degrees, and when algae cells are about to enter the red culture stage, the grown and mature algae cells are driven to swim to the red-stage culture area 6 to accumulate astaxanthin; the other part of aeration holes are in the common vertical upward direction and provide oxygen for the algae cells in the green stage culture area 7; the air holes of the two parts are uniformly distributed in a staggered way.

Optionally, each vacant zone 10 is provided with a vertical mixing aeration pipe 12, and the height of the mixing aeration pipe 12 is not lower than that of the subarea 9; the bottom of the mixing aeration pipe 12 is communicated with the carbon dioxide aeration pipe 109 and the compressed air aeration pipe 110, so that both carbon dioxide and compressed air can be introduced into the mixing aeration pipe 12; the side surfaces of the mixing aeration pipes 12 facing the subareas 9 are provided with vertical aeration holes, so that the free algae cells in the green stage in the vacant areas 10 are driven to the subareas 9 in the corresponding direction, and the quantity of the algae cells free outside the subareas 9 is reduced; in addition, the mixing aeration pipe 12 can disturb the algae liquid in the vacant zone 10, so that part of nutrients in the green-stage culture zone 7 overflow, and the air supply quantity of a small quantity of algae cells in the vacant zone 10 is increased, so that the part of algae cells can be normally produced and propagated under the proper culture condition far away from the green-stage culture zone 7.

In a specific embodiment of the present invention, a vertical mixing aeration pipe 12 is disposed in the middle of the vacant zone 10 surrounded by the three subareas 9, the height of the vertical mixing aeration pipe 12 is equal to the height of the three subareas 9, the bottom of the mixing aeration pipe 12 is communicated with a carbon dioxide aeration pipe 109 and a compressed air aeration pipe 110, the side surfaces of the mixing aeration pipe 12 facing the three subareas 9 are respectively provided with vertically arranged aeration holes, aeration is performed in the direction of the corresponding subarea 9, and the free algae cells in the green stage in the vacant zone 10 are respectively driven towards the three subareas 9, that is, the free algae cells are driven into the nearest subarea 9 according to the principle. The middle part of the vacant area 10 enclosed by the four subareas 9 is provided with a vertical mixing aeration pipe 12, which is the same as the above form, and the difference is that the side surfaces of the mixing aeration pipe 12 facing the four subareas 9 are provided with aeration holes in vertical rows.

Therefore, the volume in the reaction tank body 1 can be utilized to the maximum extent, the algae cells are promoted to accumulate astaxanthin in the red-stage culture area 6, the algae cells are cultured in the green-stage culture area 7, the algae cells are driven in the vacant area 10, meanwhile, the culture environment is provided, and the utilization rate and the culture efficiency of the reaction tank body 1 are greatly improved.

Preferably, the green-stage culture area 7 and the red-stage culture area 6 of each subarea 9 are provided with a temperature sensor 105, a density sensor 106 and a pH sensor 107, so that the culture environment conditions of different areas can be monitored in real time.

Optionally, a harvesting branch is arranged at the bottom of each red-stage culture area 6 and used for harvesting the astaxanthin-accumulated algal cells of each partition 9, and the harvesting branches of all the partitions 9 are connected with the harvesting outlet 111 through parallel pipelines.

Optionally, each red-stage culture area 6 is provided with a sampling branch for sampling algae cell samples corresponding to the subareas 9, and the sampling branches of all the subareas 9 are connected with the sampling port 112 through parallel pipelines.

Claims

1. A multi-stage collaborative algae culture reactor is characterized by comprising a reaction tank body, wherein the upper part of the reaction tank body is provided with a CIP cleaner and a liquid inlet, the middle part of the reaction tank body is provided with a plurality of LED light supplement lamps and sensors, the middle lower part of the reaction tank body is provided with a condensing coil pipe, and the bottom of the reaction tank body is provided with an aeration pipe;

2. The multi-stage collaborative algae cultivation reactor of claim 1, wherein the liquid inlet comprises a water inlet, an algae seed inlet, a nutrient solution inlet and a steam inlet, the water inlet is connected with a pure water tank to supply pure water to the reactor; the algae seed inlet is connected with an algae seed box and supplies algae seeds for the reactor when the reactor is just started; the nutrient solution inlet is connected with a nutrient solution box and supplies nutrient solution for the reactor; the steam inlet is connected with a steam generator, and when a batch of algae is cultured and algae liquid is discharged, steam is used for disinfecting the reactor.

3. The multi-stage collaborative algae cultivation reactor of claim 1, wherein the sensors comprise a temperature sensor, a density sensor, a pH sensor, and a level sensor, and the aeration tubes comprise a carbon dioxide aeration tube and a compressed air aeration tube.

4. The multi-stage collaborative algae cultivation reactor of claim 3, wherein the carbon dioxide aeration pipe is connected with a carbon dioxide flow meter, a carbon dioxide air pump and a carbon dioxide storage tank in sequence through an air pipe, and the compressed air aeration pipe is connected with a compressed air flow meter, a compressed air pump and a compressed air storage tank in sequence through an air pipe.

5. The multi-stage collaborative algae cultivation reactor of claim 1, wherein the CIP washer is connected in parallel with a pure water tank and a disinfectant tank through pipes for spraying disinfectant and washing water into the reactor; two ends of the condensing coil are respectively connected with a water cooler through pipelines;

a harvesting outlet is formed in the bottom of the reaction tank body, and the reaction tank body is sequentially connected with a liquid pump, a centrifugal machine and a product storage tank through pipelines; the middle lower part of the reaction tank body is provided with a sampling port.

6. The multi-stage cooperative algae cultivation reactor of claim 3, wherein the plurality of partitions are arranged in a plurality of circles from the inner wall of the reaction tank to the center of the reaction tank, the partitions of each circle are tangent to the partitions of the adjacent circle, and the partitions forming each circle are tangent to each other; and an irregularly-shaped vacant area is formed between the partitions of the two adjacent circles.

7. The multi-stage collaborative algae cultivation reactor according to claim 6, wherein one of the sub-areas comprises the red stage cultivation area, a shading plate and a green stage cultivation area from inside to outside, the red stage cultivation area is provided with a plurality of lamp rings from inside to outside, each lamp ring is formed by arranging a plurality of LED light supplement lamps at the same interval, and the intervals between adjacent lamp rings are equal;

the green stage is bred the district and is outwards set up a plurality of rings of lamp rings by the light screen, and every lamp ring is formed by the same distance arrangement of a plurality of LED light filling lamp interval, and the interval of adjacent lamp ring equals.

8. The multi-stage cooperative algae cultivation reactor according to claim 7, wherein the light shielding plate comprises a plurality of support rods arranged in a circle and a light shielding rolling curtain, the support rods are vertically arranged, a rolling shaft is arranged inside the support rods, the light shielding rolling curtain is wound on the rolling shaft, a rolling curtain outlet is arranged on the side surface of the support rods, the height of the rolling curtain outlet is not less than that of the light shielding rolling curtain, traction ropes are respectively arranged at the top and the bottom of one side of the light shielding rolling curtain close to the rolling curtain outlet, and the other ends of the traction ropes are respectively connected with the top and the bottom of the rolling shaft of the adjacent support rods.

9. The multi-stage collaborative algae cultivation reactor of claim 6, wherein the compressed air aeration pipe is divided into a strong aeration zone and a weak aeration zone at a portion corresponding to each division, the strong aeration zone corresponding to a red-stage cultivation zone;

the weak aeration zone corresponds to the green stage culture zone, the aeration holes of the weak aeration zone are divided into two parts, one part of the aeration holes are inclined towards the red stage culture zone, the other part of the aeration holes are normal and vertical upwards, and the air holes of the two parts are uniformly distributed in a staggered manner.

10. The multi-stage cooperative algae cultivation reactor of claim 9, wherein each of the vacant zones is provided with a vertical mixing aeration pipe having a height not lower than that of the partitioned zone; the bottom of the mixing aeration pipe is communicated with the carbon dioxide aeration pipe and the compressed air aeration pipe, so that both carbon dioxide and compressed air can be introduced into the mixing aeration pipe; the side surfaces of the mixing aeration pipes facing the subareas are provided with vertical aeration holes.