CN216090720U - Improved human nuclear genome transplantation micromanipulation needle - Google Patents
Improved human nuclear genome transplantation micromanipulation needle Download PDFInfo
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- CN216090720U CN216090720U CN202122485773.1U CN202122485773U CN216090720U CN 216090720 U CN216090720 U CN 216090720U CN 202122485773 U CN202122485773 U CN 202122485773U CN 216090720 U CN216090720 U CN 216090720U
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Abstract
The utility model discloses an improved human nuclear genome transplantation micro-operation needle, which comprises a needle tube communicated with a micro-operation tube, wherein the needle tube comprises an inclined rod, one end of the inclined rod is detachably connected with the micro-operation tube, the included angle between the inclined rod and the horizontal plane is 30-40 degrees, one end of the inclined rod, far away from the micro-operation tube, is a needle head, the inner diameter of the needle head is 5-60 mu m, one end of the needle head, far away from the inclined rod, is provided with an opening, and the length of the needle head is 0.8-1 mm; the improved human nuclear genome transplantation micromanipulation needle is mainly used for micromanipulation such as nucleus taking, nuclear transplantation, polar body taking, polar body fusion, polar body injection and the like, is designed according to the size of a spindle chromosome complex, a first polar body and a second polar body, is more convenient to operate, has smaller damage to an ovum, and further improves the survival rate of a reconstructed ovum and the embryonic development potential.
Description
Technical Field
The utility model relates to the field of human gamete and embryo micromanipulation needles, in particular to an improved human nuclear genome transplantation micromanipulation needle.
Background
In recent years, with the deep research of basic theory of human reproductive medicine and the rapid development of new technology, reproductive genetic technology derived on the basis of the research brings healthy children to families with various genetic diseases. For maternally inherited disabling lethal mitochondrial DNA genetic diseases, there has been no effective treatment or prevention. Mitochondrial replacement techniques based on nuclear genome transplantation (including spindle-chromosome complex transplantation, prokaryotic transplantation, first polar body transplantation, second polar body transplantation) hold promise for such families. Since the prior mitochondrial replacement technology research mainly focuses on animal gametes and embryos such as mice and monkeys, the size and texture difference between the animal gametes and the embryos and human gametes and embryos still exists, and invasive operations such as nucleus taking and polar body injection have high requirements on a micro-injection needle, the success degree of ICSI operation is greatly influenced by the micro-operation tool technology, and the success rate of micro-operation can be greatly improved by a proper operation tool. The micro-operation needle with accurate size, perfect shape and convenient operation of workers is just the guarantee of the success of the nuclear transplantation micro-operation.
The prior nuclear transfer needle is easy to damage the ovum because of the overlarge or undersize inner diameter in the operation process, so that an improved human nuclear genome transfer micromanipulation needle is needed to solve the problems.
SUMMERY OF THE UTILITY MODEL
The present invention aims at providing an improved human nuclear genome transplantation micromanipulation needle to solve the problems of the prior art.
In order to achieve the purpose, the utility model provides the following scheme: the utility model provides an improved human nuclear genome transplantation micro-operation needle, which comprises a needle tube communicated with a micro-operation tube, wherein the needle tube comprises an inclined rod, one end of the inclined rod is detachably connected with the micro-operation tube, an included angle between the inclined rod and the horizontal plane is 30-40 degrees, one end of the inclined rod, far away from the micro-operation tube, is a needle head, the inner diameter of the needle head is 5-60 mu m, one end of the needle head, far away from the inclined rod, is provided with an opening, and the length of the needle head is 0.8-1 mm.
Preferably, the angle between the inclined rod and the horizontal plane is 35 degrees, the length of the needle head is 1mm, the opening of the needle head is a flat opening, the inner diameter of the needle head is 13 μm, and the outer diameter of the needle head is gradually reduced from the end of the inclined rod to the opening end.
Preferably, the contained angle of down tube and horizontal plane is 35, the length of syringe needle is 0.8mm, the opening of syringe needle is the bevel connection, the internal diameter of syringe needle is 6.5 mu m, the external diameter of syringe needle by down tube end to the open end reduces gradually.
Preferably, the included angle between the inclined rod and the horizontal plane is 35 degrees, the length of the needle head is 0.8mm, the opening of the needle head is a flat opening, the inner diameter of the needle head is 55-60 μm, and the outer diameter of the needle head is 120 μm.
Preferably, the length of the bevel rod is greater than the length of the needle.
Preferably, the inner diameter of the needle is the same as the inner diameter of the bevel shaft.
Preferably, the inclined rod is inserted into the micromanipulation tube.
The utility model discloses the following technical effects: the improved human nuclear genome transplantation micromanipulation needle is mainly used for micromanipulation such as nucleus taking, nuclear transplantation, polar body taking, polar body fusion, polar body injection and the like, is designed according to the size of a spindle chromosome complex, a first polar body and a second polar body, is more convenient to operate, has smaller damage to an ovum, and further improves the survival rate of a reconstructed ovum and the embryonic development potential.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings needed to be used in the embodiments will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art to obtain other drawings without inventive exercise.
FIG. 1 is a schematic structural diagram of an improved human nuclear genome transplantation micromanipulation needle according to the present invention;
FIG. 2 is an enlarged view of a portion of FIG. 1;
FIG. 3 is a schematic structural view of example 2;
FIG. 4 is a schematic structural view of embodiment 3;
wherein: 2. a diagonal bar; 3. a needle.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
In order to make the aforementioned objects, features and advantages of the present invention comprehensible, embodiments accompanied with figures are described in further detail below.
Example 1
Referring to fig. 1-2, the utility model provides an improved human nuclear genome transplantation micro-operation needle, which comprises a needle tube communicated with a micro-operation tube, wherein the needle tube comprises an inclined rod 2, one end of the inclined rod 2 is detachably connected with the micro-operation tube, an included angle between the inclined rod 2 and a horizontal plane is 30-40 degrees, one end of the inclined rod 2, which is far away from the micro-operation tube, is a needle head 3, the inner diameter of the needle head 3 is 5-60 micrometers, one end of the needle head 3, which is far away from the inclined rod 2, is provided with an opening, and the length of the needle head 3 is 0.8-1 mm.
The improved human nuclear genome transplantation micro-operation needle is mainly used for nucleus taking, nuclear transplantation, pole body taking, pole body fusion and other works, is designed according to the size of a spindle chromosome complex, a first pole body and a second pole body, has smaller damage to an ovum and further improves the survival rate of the ovum.
In a further optimized scheme, the length of the inclined rod 2 is greater than that of the needle 3.
In a further optimized scheme, the inner diameter of the needle head 3 is the same as that of the inclined rod 2.
According to the further optimized scheme, the inclined rod 2 is inserted into the micro-operation tube, and one end, close to the micro-operation tube, of the inclined rod 2 is directly inserted into the micro-operation tube when the micro-operation tube is used.
In a further optimization scheme, when the improved human nuclear genome transplantation micro-operation needle is a nuclear transplantation needle, the inner diameter of the nuclear transplantation needle is changed into 13 mu m according to the size of a spindle chromosome compound, a first polar body and a second polar body, the damage to eggs is small, the included angle between the inclined rod 2 and the horizontal plane is 35 degrees, the length of the needle head 3 is 1mm, the outer diameter of the needle head 3 is gradually reduced from the end of the inclined rod 2 to the open end, the operation of nucleus/polar body taking and transplantation is convenient, the nucleus/polar body is not easy to lose, the opening of the needle head 3 is a flat opening, and the damage to the eggs is reduced.
Example 2
Referring to fig. 3, when the improved human nuclear genome transplantation micro-needle of the present invention is a polar body injection needle, in order to facilitate the injection of the polar body, an angle between the inclined rod 2 and the horizontal plane is 35 °, the length of the needle 3 is 0.8mm, the opening of the needle 3 is an inclined opening, the inner diameter of the needle 3 is 6.5 μm, and the outer diameter of the needle 3 is gradually reduced from the end of the inclined rod 2 to the opening end, so that the loss of the polar body can be reduced, and the ovum cannot be damaged due to the too coarse inner diameter.
Example 3
Referring to fig. 4, when the improved human nuclear genome transplantation micromanipulation needle of the present invention is an ovum holding needle, in order to fix the ovum, an included angle between the diagonal rod 2 and the horizontal plane is 35 °, the length of the needle head 3 is 0.8mm, the opening of the needle head 3 is a flat mouth, the inner diameter of the needle head 3 is 55 to 60 μm, the outer diameter of the needle head 3 is 120 μm, the inner diameter of the ovum holding needle is increased, the fixing effect on the ovum is better, and the damage is smaller.
In the description of the present invention, it is to be understood that the terms "longitudinal", "lateral", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", and the like, indicate orientations or positional relationships based on those shown in the drawings, are merely for convenience of description of the present invention, and do not indicate or imply that the referenced devices or elements must have a particular orientation, be constructed and operated in a particular orientation, and thus, are not to be construed as limiting the present invention.
The above-described embodiments are merely illustrative of the preferred embodiments of the present invention, and do not limit the scope of the present invention, and various modifications and improvements of the technical solutions of the present invention can be made by those skilled in the art without departing from the spirit of the present invention, and the technical solutions of the present invention are within the scope of the present invention defined by the claims.
Claims (7)
1. An improved human nuclear genome transplantation micromanipulation needle, which is characterized in that: the needle tube comprises a needle tube communicated with a micro-operation tube, wherein the needle tube comprises an inclined rod (2), one end of the inclined rod (2) is detachably connected with the micro-operation tube, the inclined rod (2) and a horizontal plane form an included angle of 30-40 degrees, one end of the micro-operation tube, far away from the inclined rod (2), is a needle head (3), the inner diameter of the needle head (3) is 5-60 mu m, one end of the needle head (3), far away from the inclined rod (2), is provided with an opening, and the length of the needle head (3) is 0.8-1 mm.
2. The improved human nuclear genome transplantation micromanipulation needle of claim 1, wherein: the contained angle of down tube (2) and horizontal plane is 35, the length of syringe needle (3) is 1mm, the opening of syringe needle (3) is the flat mouth, the internal diameter of syringe needle (3) is 13 μm, the external diameter of syringe needle (3) by down tube (2) end extremely the open end reduces gradually.
3. The improved human nuclear genome transplantation micromanipulation needle of claim 1, wherein: the contained angle of down tube (2) and horizontal plane is 35, the length of syringe needle (3) is 0.8mm, the opening of syringe needle (3) is the bevel connection, the internal diameter of syringe needle (3) is 6.5 mu m, the external diameter of syringe needle (3) by down tube (2) end extremely the open end reduces gradually.
4. The improved human nuclear genome transplantation micromanipulation needle of claim 1, wherein: the contained angle of down tube (2) and horizontal plane is 35, the length of syringe needle (3) is 0.8mm, the opening of syringe needle (3) is the flat mouth, the internal diameter of syringe needle (3) is 55 ~ 60 mu m, the external diameter of syringe needle (3) is 120 mu m.
5. The improved human nuclear genome transplantation micromanipulation needle of claim 1, wherein: the length of the inclined rod (2) is greater than that of the needle head (3).
6. The improved human nuclear genome transplantation micromanipulation needle of claim 1, wherein: the inner diameter of the needle head (3) is the same as that of the inclined rod (2).
7. The improved human nuclear genome transplantation micromanipulation needle of claim 1, wherein: the inclined rod (2) is inserted with the micro-operation tube.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202122485773.1U CN216090720U (en) | 2021-10-15 | 2021-10-15 | Improved human nuclear genome transplantation micromanipulation needle |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202122485773.1U CN216090720U (en) | 2021-10-15 | 2021-10-15 | Improved human nuclear genome transplantation micromanipulation needle |
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| Publication Number | Publication Date |
|---|---|
| CN216090720U true CN216090720U (en) | 2022-03-22 |
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| CN202122485773.1U Active CN216090720U (en) | 2021-10-15 | 2021-10-15 | Improved human nuclear genome transplantation micromanipulation needle |
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| CN (1) | CN216090720U (en) |
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2021
- 2021-10-15 CN CN202122485773.1U patent/CN216090720U/en active Active
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