CN204589203U - A kind of body-cell neucleus transplanting reconstructed embryo merges electrode - Google Patents
A kind of body-cell neucleus transplanting reconstructed embryo merges electrode Download PDFInfo
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- CN204589203U CN204589203U CN201520234850.8U CN201520234850U CN204589203U CN 204589203 U CN204589203 U CN 204589203U CN 201520234850 U CN201520234850 U CN 201520234850U CN 204589203 U CN204589203 U CN 204589203U
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- reconstructed embryo
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Abstract
The utility model discloses a kind of body-cell neucleus transplanting reconstructed embryo and merge electrode, it relates to Mammals breeding and technical field of animal reproduction.It comprises electrode insulation bar, electrode supply coupling end, electrode inboardend, stainless steel electrode and electrode and merges end, the front end of electrode insulation bar is fixed with stainless steel electrode by electrode inboardend, the front end of stainless steel electrode is that electrode merges end, the rear end of stainless steel electrode is electrode supply coupling end, electrode supply coupling end is positioned at electrode insulation bar hollow 18mm place, and the rear end of electrode insulation bar is fixed on micrurgy instrument.The utility model simplifies the difficulty that reconstructed embryo merges, and improves fusion efficiencies, significant for raising nucleus transplantation efficiency.
Description
Technical field
The utility model relates to Mammals breeding and technical field of animal reproduction, be specifically related to a kind of body-cell neucleus transplanting reconstructed embryo and merge electrode.
Background technology
Body-cell neucleus transplanting is also known as somatic cell clone, namely somatic cell nuclear is moved in enucleation oocyte, make it recombinate and can grow for new embryo, this embryo finally grows for animal individual, cloned animal is called with the animal that method of nuclear transfer obtains, due to the differentiation of somatocyte height, recovery totipotency difficulty, namely the principle of body-cell neucleus transplanting is nuclear totipotency, along with nuclear transplantation research steps is constantly accelerated, people achieve great successes in animal cloning research field, more and more somatic cell clone animal is born in succession, demonstrate well differentiated somatocyte in ovocyte matter after reprogrammed, the cytocerastic totipotency of its embryo support can be recovered.
So far, nuclear transplantation inefficiency is the bottleneck of its development of restriction always, such as classical micromanipulator is expensive, professional technique difficulty is higher, cloning efficiency is very low, clone embryos abortion ratio is high, perinatal mortality is high, after birth, individual surviving rate is low, survival offspring is physically different etc., these all significantly limit the cultivation that clone technology is planted excellent poultry, the structure of bio-reactor, high added value transgenic animal produce, animal plasm resource protection, the foundation of disease model, the agriculturals such as regenerative medicine, the widespread use in biotechnology and the multiple field of medical science.
In order to solve the problem, design a kind of body-cell neucleus transplanting reconstructed embryo and merging electrode or necessary.
Utility model content
For the deficiency that prior art exists, the utility model object is to provide a kind of body-cell neucleus transplanting reconstructed embryo to merge electrode, and structure is simple, reasonable in design, simplify the difficulty that reconstructed embryo merges, improve fusion efficiencies, significant for raising nucleus transplantation efficiency.
To achieve these goals, the utility model realizes by the following technical solutions: a kind of body-cell neucleus transplanting reconstructed embryo merges electrode, comprise electrode insulation bar, electrode supply coupling end, electrode inboardend, stainless steel electrode and electrode and merge end, the front end of electrode insulation bar is fixed with stainless steel electrode by electrode inboardend, the front end of stainless steel electrode is that electrode merges end, the rear end of stainless steel electrode is electrode supply coupling end, electrode supply coupling end is positioned at electrode insulation bar hollow 18mm place, and the rear end of electrode insulation bar is fixed on micrurgy instrument.
As preferably, described electrode insulation bar is made up of inner hollow duroplasts material, and length is 120mm, and diameter is 4.59mm, and internal diameter is 2mm.
As preferably, described electrode supply coupling end connects fusion instrument power supply, connects power supply place V-shaped; Described electrode inboardend adopts stainless steel electrode inboardend, and diameter is 2.1mm.
As preferably, described stainless steel electrode diameter is 0.49mm, and merging end to the entire length of electrode supply coupling end from electrode is 50mm, and stainless steel electrode adopts Z-shaped or L shape electrode.
As preferably, the diameter that described electrode merges end is 0.02mm.
The beneficial effects of the utility model: simplify the difficulty that reconstructed embryo merges, operation is simpler, improves fusion efficiencies, significant for raising nucleus transplantation efficiency.
Accompanying drawing explanation
The utility model is described in detail below in conjunction with the drawings and specific embodiments;
Fig. 1 is structural representation of the present utility model.
Embodiment
The technique means realized for making the utility model, creation characteristic, reaching object and effect is easy to understand, below in conjunction with embodiment, setting forth the utility model further.
With reference to Fig. 1, this embodiment is by the following technical solutions: a kind of body-cell neucleus transplanting reconstructed embryo merges electrode, comprise electrode insulation bar 1, electrode supply coupling end 2, electrode inboardend 3, stainless steel electrode 4 and electrode and merge end 5, the front end of electrode insulation bar 1 is fixed with stainless steel electrode 4 by electrode inboardend 3, the front end of stainless steel electrode 4 is that electrode merges end 5, the rear end of stainless steel electrode 4 is electrode supply coupling end 2, electrode supply coupling end 2 is positioned at electrode insulation bar 1 hollow 18mm place, for connecting fusion instrument power supply, connect power supply place V-shaped.
It should be noted that described electrode insulation bar 1 is made up of inner hollow duroplasts material, length is 120mm, diameter is 4.59mm, internal diameter is 2mm, and the front end of electrode insulation bar 1 connects stainless steel electrode 4, and rear end is mainly used in being fixed on micrurgy instrument when electrode merges.
Described electrode inboardend 3 is made up of stainless steel, and diameter is 2.1mm, and after fixed electorde, front end is that electrode merges end 5, and rear end is electrode power-line coupling end 2, is connected to electrode insulation bar 1 after stainless steel electrode inboardend 3 fixed electorde.
Described stainless steel electrode 4 diameter is 0.49mm, and be 50mm from electrode fusion end 5 to the entire length of electrode supply coupling end 2, stainless steel electrode 4 can make Z-shaped or L shape according to matching used fiber operations instrument.
In addition, it is point diameter 0.02mm that described electrode merges that stainless steel electrode 4 that end 5 is 0.49mm by diameter polishes, and merges for fixing reconstructed embryo.
When this embodiment uses, first the electrode insulation bar 1 of two electrodes is fixed on micrurgy instrument, electrode supply coupling end 2 is connected with fusion instrument power supply, electro' asion liquid droplet is put into by needing the recombinant chou merged, under the optical frames of micrurgy instrument, operate two electrodes by operation handle arm and coordinate, make its electrode merge end 5 and block recombinant chou, and carry out electro' asion by the pulse output of fusion instrument, very easy.
This embodiment is improved merging difficult problem in body-cell neucleus transplanting reconstructed embryo electro' asion process, simplifies and merges difficulty, improve fusion efficiencies, have wide market application foreground.
More than show and describe ultimate principle of the present utility model and principal character and advantage of the present utility model.The technician of the industry should understand; the utility model is not restricted to the described embodiments; what describe in above-described embodiment and specification sheets just illustrates principle of the present utility model; under the prerequisite not departing from the utility model spirit and scope; the utility model also has various changes and modifications, and these changes and improvements all fall within the scope of claimed the utility model.The claimed scope of the utility model is defined by appending claims and equivalent thereof.
Claims (6)
1. a body-cell neucleus transplanting reconstructed embryo merges electrode, it is characterized in that, comprise electrode insulation bar (1), electrode supply coupling end (2), electrode inboardend (3), stainless steel electrode (4) and electrode merge end (5), the front end of electrode insulation bar (1) is fixed with stainless steel electrode (4) by electrode inboardend (3), the front end of stainless steel electrode (4) is that electrode merges end (5), the rear end of stainless steel electrode (4) is electrode supply coupling end (2), electrode supply coupling end (2) is positioned at electrode insulation bar (1) hollow 18mm place, the rear end of electrode insulation bar (1) is fixed on micrurgy instrument.
2. a kind of body-cell neucleus transplanting reconstructed embryo according to claim 1 merges electrode, and it is characterized in that, described electrode insulation bar (1) is made up of inner hollow duroplasts material, and length is 120mm, and diameter is 4.59mm, and internal diameter is 2mm.
3. a kind of body-cell neucleus transplanting reconstructed embryo according to claim 1 merges electrode, it is characterized in that, described electrode supply coupling end (2) connects fusion instrument power supply, connects power supply place V-shaped.
4. a kind of body-cell neucleus transplanting reconstructed embryo according to claim 1 merges electrode, it is characterized in that, described electrode inboardend (3) adopts stainless steel electrode inboardend, and diameter is 2.1mm.
5. a kind of body-cell neucleus transplanting reconstructed embryo according to claim 1 merges electrode, it is characterized in that, described stainless steel electrode (4) diameter is 0.49mm, merging end (5) to the entire length of electrode supply coupling end (2) from electrode is 50mm, and stainless steel electrode (4) adopts Z-shaped or L shape electrode.
6. a kind of body-cell neucleus transplanting reconstructed embryo according to claim 1 merges electrode, it is characterized in that, the diameter that described electrode merges end (5) is 0.02mm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520234850.8U CN204589203U (en) | 2015-04-13 | 2015-04-13 | A kind of body-cell neucleus transplanting reconstructed embryo merges electrode |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520234850.8U CN204589203U (en) | 2015-04-13 | 2015-04-13 | A kind of body-cell neucleus transplanting reconstructed embryo merges electrode |
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| Publication Number | Publication Date |
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| CN204589203U true CN204589203U (en) | 2015-08-26 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201520234850.8U Expired - Fee Related CN204589203U (en) | 2015-04-13 | 2015-04-13 | A kind of body-cell neucleus transplanting reconstructed embryo merges electrode |
Country Status (1)
| Country | Link |
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| CN (1) | CN204589203U (en) |
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2015
- 2015-04-13 CN CN201520234850.8U patent/CN204589203U/en not_active Expired - Fee Related
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20150826 Termination date: 20160413 |
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| CF01 | Termination of patent right due to non-payment of annual fee |