CN215302156U - Chinese-medicinal material tissue culture equipment - Google Patents

Chinese-medicinal material tissue culture equipment Download PDF

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Publication number
CN215302156U
CN215302156U CN202121609930.9U CN202121609930U CN215302156U CN 215302156 U CN215302156 U CN 215302156U CN 202121609930 U CN202121609930 U CN 202121609930U CN 215302156 U CN215302156 U CN 215302156U
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plate
incubator
culture
vibration
tissue culture
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杨光喜
蒲小华
陈蓉
汤云富
杨利民
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Huaihua Dafengyuan Ecological Agriculture Development Co ltd
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Huaihua Dafengyuan Ecological Agriculture Development Co ltd
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Abstract

The utility model discloses a traditional Chinese medicine tissue culture device which comprises an incubator and a transparent sealing cover hinged to the incubator, wherein the incubator is connected with a middle plate, an installation space is formed between the middle plate and the bottom plate of the incubator, a culture space is formed between the middle plate and a top plate of the incubator, a vibration mechanism is arranged in the installation space, a clamping mechanism is connected above the vibration mechanism, the clamping mechanism is positioned in the culture space, an illuminating lamp, a warm air blower and a temperature sensor are arranged in the culture space, and the bottom plate of the incubator is connected with an L plate; the utility model has simple structure, low cost and convenient installation and disassembly, improves the culture efficiency and the oxygen content in the culture device by vibration culture, thereby leading the tissue propagation of the traditional Chinese medicine to be more uniform and the propagation period to be shorter; the incubator that can the centre gripping adaptation unidimensional model, improved the universality, centre gripping easy operation, the centre gripping is stable, has improved the practicality.

Description

Chinese-medicinal material tissue culture equipment
Technical Field
The utility model belongs to the technical field of tissue culture equipment, and particularly relates to traditional Chinese medicine tissue culture equipment.
Background
The aseptic culture technology of medicinal plant or clone technology of medicinal plant is characterized by that according to the theory of that plant cell possesses totipotency, it utilizes the isolated organ, tissue or cell of medicinal plant body, such as root, stem, flower, leaf, fruit, seed, embryo, pollen, parenchyma and vascular bundle tissue, etc. under the conditions of aseptic and proper artificial culture device and illumination and temp. the process of inducing callus, adventitious bud and adventitious root, finally differentiating and growing to form complete plant is called isolated culture or test tube culture, and said culture mode possesses the following advantages: 1. plants are obtained by rapid propagation, and the propagation coefficient is high; 2. the method is not limited by seasons, and can be used for annual large-scale industrial production; 3. fast growth, short period and strong repeatability.
In cultivateing chinese-medicinal material tissue in-process, the mode of accessible vibrations culture apparatus makes nutrient solution oxygen content improve in the culture apparatus, and help chinese-medicinal material tissue and nutrient solution fully contact, the improvement of oxygen content and the full contact of nutrient solution help chinese-medicinal material tissue to breed the differentiation, the culture apparatus of current vibrations formula is not convenient for observe the tissue of cultivateing indoor, also hardly adapt to the culture apparatus of different size of a dimension, and the structure is complicated, the cost is higher, a simple structure is provided now, the cost is lower, and the chinese-medicinal material tissue culture apparatus that the practicality is high.
SUMMERY OF THE UTILITY MODEL
In view of the problems raised by the above background art, the present invention is directed to: aims to provide a traditional Chinese medicine tissue culture device.
In order to realize the technical purpose, the Chinese medicinal material tissue culture equipment adopts the following technical scheme:
a traditional Chinese medicine tissue culture device comprises an incubator and a transparent sealing cover hinged to the incubator, wherein the incubator is connected with a middle plate, an installation space is formed between the middle plate and the incubator bottom plate, a culture space is formed between the middle plate and an incubator top plate, a vibration mechanism is arranged in the installation space, a clamping mechanism is connected above the vibration mechanism, the clamping mechanism is located in the culture space, an illuminating lamp, a warm air blower and a temperature sensor are arranged in the culture space, and the incubator bottom plate is connected with an L plate;
the vibration mechanism comprises a motor and a plurality of slide rails, the slide rails are connected with slide blocks, an arc plate is connected between the slide blocks, one side of the arc plate is connected with a plurality of springs, one end of each spring is fixedly connected with the arc plate, the other end of each spring is fixedly connected with an L plate, a guide rod is arranged in each spring and fixedly connected with the L plate, the guide rods are not in contact with the arc plates, and the output end of the motor is connected with a vibration generating plate;
fixture is including cultivateing the board, it is equipped with the holder groove of symmetric distribution to cultivate the board, sliding mounting has the clamp splice in the holder groove, the clamp splice is equipped with cambered surface and recess, it is equipped with the ear seat to cultivate the board both sides, the ear seat is connected with the stock, the stock both ends respectively with ear seat, clamp splice threaded connection, the clamp splice both sides are connected with respectively and are drawn spring and compression spring, it is equipped with the limiting plate to cultivate the board, the limiting plate matches with the recess, limiting plate length is less than the length in holder groove.
Further inject, the board takes place to shake is similar to triangle-shaped, three angles of board takes place to shake are circular-arc, such structural design for when the board takes place to shake rotatory, the arc angle of the board takes place to shake can act on the radian board, thereby makes radian board extrusion spring retreat, when the arc angle does not act on the radian board, the spring resets again, ejecting radian board, thereby makes the radian board can the seesaw.
Further inject, the inside bottom surface of incubator is equipped with the spacing groove, the appearance matching of spacing groove and L board, slide rail, such structural design has directly confirmed the mounted position of L board and slide rail in the incubator through the spacing groove.
Further inject, the incubator is equipped with the installation piece, the medium plate is formed by preceding medium plate and the concatenation of back medium plate, preceding medium plate and back medium plate are the mirror image structure, back medium plate is equipped with the hole of stepping down, preceding medium plate and back medium plate are connected with the incubator through the installation piece, and the mounted position of preceding medium plate and back medium plate is confirmed through the installation piece to such structural design, through the concatenation design of preceding medium plate and back medium plate, makes fixture install more easily on vibrations mechanism.
Further inject, the incubator bottom surface is equipped with the spout, the incubator side is equipped with the groove of stepping down, the incubator is connected with the shrouding through the spout with the groove of stepping down, such structural design for the shrouding that is used for sealed installation space is easier to assemble with the incubator.
The utility model has the beneficial effects that:
1. the structure is simple, the cost is low, the installation and the disassembly are convenient, the culture efficiency and the oxygen content in the culture device are improved through vibration culture, so that the tissue propagation of the traditional Chinese medicine is more uniform, and the propagation period is shorter;
2. the incubator that can the centre gripping adaptation unidimensional model, improved the universality, centre gripping easy operation, the centre gripping is stable, has improved the practicality.
Drawings
The utility model is further illustrated by the non-limiting examples given in the accompanying drawings;
FIG. 1 is a schematic structural diagram of an embodiment of a tissue culture apparatus for Chinese herbs according to the present invention;
FIG. 2 is a schematic structural view of an embodiment of a Chinese medicinal material tissue culture apparatus according to the present invention with the clamping mechanism removed;
FIG. 3 is a schematic front view of an embodiment of a Chinese medicinal material tissue culture apparatus according to the present invention with the clamping mechanism removed;
FIG. 4 is a schematic view of an internal structure of an embodiment of a tissue culture apparatus for Chinese herbs according to the present invention;
FIG. 5 is a schematic structural diagram of an incubator in an embodiment of the tissue culture apparatus for Chinese medicinal materials of the present invention;
FIG. 6 is a schematic structural diagram of a middle plate in an embodiment of a tissue culture apparatus for Chinese herbs according to the present invention;
FIG. 7 is a schematic structural diagram of a clamping mechanism in an embodiment of the tissue culture apparatus for traditional Chinese medicinal materials of the present invention;
FIG. 8 is an enlarged view of a portion of the tissue culture apparatus for traditional Chinese herbs at A in an embodiment of the present invention;
the main element symbols are as follows:
the incubator comprises an incubator 1, a limiting groove 101, a mounting block 103, a sliding groove 104, an abdicating groove 105, a middle plate 2, a rear middle plate 21, an abdicating hole 211, a front middle plate 22, a vibration mechanism 3, a sliding rail 31, a sliding block 32, a motor 33, a vibration generating plate 34, a guide rod 35, a spring 36, a radian plate 37, an L plate 4, a sealing plate 5, a temperature sensor 6, an illuminating lamp 7, a warm air blower 8, a clamping mechanism 9, an ear seat 901, a long rod 902, a clamping block 903, a cambered surface 9031, a groove 9032, a compression spring 904, a tension spring 905, a limiting plate 906 and a culture plate 907.
Detailed Description
In order that those skilled in the art can better understand the present invention, the following technical solutions are further described with reference to the accompanying drawings and examples.
As shown in fig. 1-8, the traditional Chinese medicine tissue culture equipment of the present invention comprises an incubator 1 and a transparent sealing cover hinged to the incubator 1, wherein the incubator 1 is connected with a middle plate 2, an installation space is formed between the middle plate 2 and a bottom plate of the incubator 1, a culture space is formed between the middle plate 2 and a top plate of the incubator 1, a vibration mechanism 3 is arranged in the installation space, a clamping mechanism 9 is connected above the vibration mechanism 3, the clamping mechanism 9 is located in the culture space, the culture space is provided with an illuminating lamp 7, a warm air blower 8 and a temperature sensor 6, and the bottom plate of the incubator 1 is connected with an L plate 4;
the vibration mechanism 3 comprises a motor 33 and a plurality of slide rails 31, the slide rails 31 are connected with slide blocks 32, radian plates 37 are connected between the slide blocks 32, one side of each radian plate 37 is connected with a plurality of springs 36, one end of each spring 36 is fixedly connected with the radian plate 37, the other end of each spring 36 is fixedly connected with the L plate 4, guide rods 35 are arranged inside the springs 36, the guide rods 35 are fixedly connected with the L plate 4, the guide rods 35 are not in contact with the radian plates 37, and the output end of the motor 33 is connected with a vibration generation plate 34;
the clamping mechanism 9 comprises a culture plate 907, the culture plate 907 is provided with symmetrically distributed clamping seat grooves, clamping blocks 903 are arranged in the clamping seat grooves in a sliding mode, the clamping blocks 903 are provided with cambered surfaces 9031 and grooves 9032, ear seats 901 are arranged on two sides of the culture plate 907, the ear seats 901 are connected with long rods 902, two ends of each long rod 902 are respectively in threaded connection with the ear seats 901 and the clamping blocks 903, two sides of each clamping block 903 are respectively connected with a tension spring 905 and a compression spring 904, the culture plate 907 is provided with a limiting plate 906, the limiting plate 906 is matched with the grooves 9032, and the length of the limiting plate 906 is smaller than that of the clamping seat grooves.
In the implementation of the scheme, the incubator for placing the traditional Chinese medicine tissues is clamped by the clamping mechanism 9, firstly, the culture personnel outwards expands the clamping blocks 903, the tension spring 905 is pulled open, the compression spring 904 is compressed, a sufficient placing space is formed between the clamping blocks 903 for placing the incubator, after the culture personnel loosens the clamping blocks 903, the clamping blocks 903 clamp the incubator under the combined action of the tension spring 905 and the compression spring 904, the clamping force is provided by elastic connection, so the clamping distance of the clamping blocks 903 can be changed according to the size change of the incubator, in order to ensure that the tension spring 905 and the compression spring 904 cannot generate secondary deformation in subsequent shaking, the ear seat 901 and the clamping blocks 903 are connected through the long rod 902, so that the clamping blocks 903 are always fixed in place after adapting to the size of the incubator, in order to ensure that the clamping blocks 903 can only horizontally slide, through the matching of the limiting plate 906 and the grooves 9032, the freedom degree of the clamping block 903 is regulated, and the length of the limiting plate 906 is smaller than that of the clamping seat groove so that the clamping block 903 can be put in or taken out of the clamping seat groove (for convenience of observation, the long rod 902 and the limiting plate 906 on one side are hidden in the drawing);
after clamping, carrying out vibration culture, starting a motor 33, driving a vibration generation plate 34 to rotate, starting the vibration generation plate 34 to intermittently extrude a radian plate 37, starting the radian plate 37 to intermittently extrude a spring 36, and driving the radian plate 37 to move back and forth left and right on a slide rail 31 through a slide block 32 under the action of the spring 36 and the vibration generation plate 34, wherein the radian plate 37 moves back and forth to drive a clamping mechanism 9 to move back and forth, so that the incubator moves back and forth, nutrient solution in the incubator is fully fused with air, the oxygen content is increased, and the traditional Chinese medicine tissues are fully contacted with culture solution in the incubator, so that the traditional Chinese medicine tissues are uniformly propagated, and the propagation efficiency is also improved;
in the process of vibration culture of the traditional Chinese medicine tissues, the warm air blower 8 is also started to raise the temperature in the incubator 1 to the preset temperature, the temperature sensor 6 can feed back information of the temperature in the incubator 1, and the illuminating lamp 7 provides a good observation visual field and illumination conditions.
Preferably, the vibration generating plate 34 is approximately triangular, and three corners of the vibration generating plate 34 are arc-shaped, so that when the vibration generating plate 34 rotates, the arc angle of the vibration generating plate 34 can act on the arc plate 37, so that the arc plate 37 extrudes the spring 36 to move backward, and when the arc angle does not act on the arc plate 37, the spring 36 resets again, and pushes out the arc plate 37, so that the arc plate 37 can move forward and backward. In fact, other configurations of the shock-generating plate 34 are specifically contemplated as appropriate.
Preferably, the bottom surface in the incubator 1 is provided with a limit groove 101, and the limit groove 101 matches with the shapes of the L-plate 4 and the slide rail 31, so that the installation positions of the L-plate 4 and the slide rail 31 in the incubator 1 are directly determined by the limit groove 101. In fact, other structural shapes of the incubator 1 for determining the positions of the L-plate 4 and the slide rail 31 may be specifically considered according to the specific situation.
Preferably, the incubator 1 is provided with the mounting block 103, the middle plate 2 is formed by splicing a front middle plate 22 and a rear middle plate 21, the front middle plate 22 and the rear middle plate 21 are in mirror image structures, the rear middle plate 21 is provided with a abdicating hole 211, the front middle plate 22 and the rear middle plate 21 are connected with the incubator 1 through the mounting block 103, the mounting positions of the front middle plate 22 and the rear middle plate 21 are determined through the mounting block 103, and the clamping mechanism 9 is more easily mounted on the vibration mechanism 3 through the splicing design of the front middle plate 22 and the rear middle plate 21. In fact, other configurations of the middle plate 2 may be specifically considered as the case may be.
Preferably, 1 bottom surfaces of incubator are equipped with spout 104, and 1 side of incubator is equipped with the groove of stepping down 105, and incubator 1 is connected with shrouding 5 through spout 104 and the groove of stepping down 105, such structural design for shrouding 5 for sealed installation space assembles with incubator 1 more easily. In fact, other configurations of the closure plate 5 and the incubator 1 may be specifically contemplated, as the case may be.
The foregoing embodiments are merely illustrative of the principles of the present invention and its efficacy, and are not to be construed as limiting the utility model. Any person skilled in the art can modify or change the above-mentioned embodiments without departing from the spirit and scope of the present invention. Accordingly, it is intended that all equivalent modifications or changes which can be made by those skilled in the art without departing from the spirit and technical spirit of the present invention be covered by the claims of the present invention.

Claims (5)

1. The utility model provides a chinese-medicinal material tissue culture equipment, incubator (1) and articulate in the transparent sealed cowling of this incubator (1), its characterized in that: the incubator (1) is connected with a middle plate (2), an installation space is formed between the middle plate (2) and a bottom plate of the incubator (1), an incubation space is formed between the middle plate (2) and a top plate of the incubator (1), a vibration mechanism (3) is arranged in the installation space, a clamping mechanism (9) is connected above the vibration mechanism (3), the clamping mechanism (9) is located in the incubation space, the incubation space is provided with an illuminating lamp (7), a warm air blower (8) and a temperature sensor (6), and the bottom plate of the incubator (1) is connected with an L plate (4);
the vibration mechanism (3) comprises a motor (33) and a plurality of sliding rails (31), the sliding rails (31) are connected with sliding blocks (32), arc plates (37) are connected between the sliding blocks (32), one sides of the arc plates (37) are connected with a plurality of springs (36), one ends of the springs (36) are fixedly connected with the arc plates (37), the other ends of the springs (36) are fixedly connected with an L plate (4), guide rods (35) are arranged inside the springs (36), the guide rods (35) are fixedly connected with the L plate (4), the guide rods (35) are not in contact with the arc plates (37), and the output end of the motor (33) is connected with a vibration generation plate (34);
the clamping mechanism (9) comprises a culture plate (907), a square block used for being connected with the vibration mechanism (3) is arranged below the culture plate (907), clamp seat grooves which are symmetrically distributed are formed in the culture plate (907), clamping blocks (903) are arranged in the clamp seat grooves in a sliding mode, cambered surfaces (9031) and grooves (9032) are formed in the clamping blocks (903), lug seats (901) are arranged on two sides of the culture plate (907), long rods (902) are connected with the lug seats (901), the clamping blocks (903) are in threaded connection with two ends of each long rod (902), tension springs (905) and compression springs (904) are connected to two sides of each clamping block (903) respectively, a limiting plate (906) is arranged on the culture plate (907), the limiting plate (906) is matched with the grooves (9032), and the length of the limiting plate (906) is smaller than that of the clamp seat grooves.
2. The traditional Chinese medicine tissue culture equipment of claim 1, wherein: the vibration generating plate (34) is approximately triangular, and three corners of the vibration generating plate (34) are arc-shaped.
3. The tissue culture apparatus for traditional Chinese medicinal materials according to claim 2, wherein: the inside bottom surface of incubator (1) is equipped with spacing groove (101), spacing groove (101) match with the appearance of L board (4), slide rail (31).
4. The tissue culture apparatus for traditional Chinese medicinal materials according to claim 3, wherein: incubator (1) is equipped with installation piece (103), well board (2) are formed by preceding well board (22) and back well board (21) concatenation, preceding well board (22) and back well board (21) are the mirror image structure, back well board (21) are equipped with hole of stepping down (211), preceding well board (22) and back well board (21) are connected with incubator (1) through installation piece (103).
5. The tissue culture apparatus for traditional Chinese medicinal materials according to claim 4, wherein: incubator (1) bottom surface is equipped with spout (104), incubator (1) side is equipped with the groove of stepping down (105), incubator (1) is connected with shrouding (5) through spout (104) and the groove of stepping down (105).
CN202121609930.9U 2021-07-15 2021-07-15 Chinese-medicinal material tissue culture equipment Active CN215302156U (en)

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CN202121609930.9U CN215302156U (en) 2021-07-15 2021-07-15 Chinese-medicinal material tissue culture equipment

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Application Number Priority Date Filing Date Title
CN202121609930.9U CN215302156U (en) 2021-07-15 2021-07-15 Chinese-medicinal material tissue culture equipment

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CN215302156U true CN215302156U (en) 2021-12-28

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114342809A (en) * 2022-02-22 2022-04-15 山西农业大学高粱研究所 Sorghum breeding callus culture apparatus

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114342809A (en) * 2022-02-22 2022-04-15 山西农业大学高粱研究所 Sorghum breeding callus culture apparatus

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