CN216614683U - Salmonidae roe fixing device for microinjection - Google Patents
Salmonidae roe fixing device for microinjection Download PDFInfo
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- CN216614683U CN216614683U CN202220112269.9U CN202220112269U CN216614683U CN 216614683 U CN216614683 U CN 216614683U CN 202220112269 U CN202220112269 U CN 202220112269U CN 216614683 U CN216614683 U CN 216614683U
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- fixing groove
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- salmonidae
- base
- microinjection
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Abstract
The utility model discloses a Salmonidae roe fixing device for microinjection, which comprises a base, wherein two sides of the top end of the base are fixedly connected with a support frame, a round fixing groove is arranged at the top end of the base and positioned between the two support frames, the round fixing groove is internally provided with a agar layer, the top end of the base is provided with a limiting piece, the round fixing groove is detachably connected with the base through the limiting piece, the supporting frame is provided with an extrusion part which is positioned above the circular fixing groove and is arranged corresponding to the circular fixing groove, the supporting frame is fixedly connected with a motor, the motor is in transmission connection with the extrusion part, a constant-temperature heat-insulation plate is arranged in the circular fixing groove, the circular fixing groove is provided with a temperature adjusting device, and the constant-temperature heat insulation plate is electrically connected with the temperature adjusting device. The utility model has simple structure and convenient operation, and greatly improves the efficiency of microinjection of salmonidae roes.
Description
Technical Field
The utility model relates to the technical field of roe microinjection, in particular to a Salmonidae roe fixing device for microinjection.
Background
When the microinjection operation is carried out on salmonidae fishes in a laboratory, the roes in the cell stage are injected through the microinjection with extremely fine tube tips, so that the researches of transient overexpression and knockout of genes, preparation of transgenes or mutant lines and the like are realized. The prior art for fixing the roe of the salmonidae fishes by microinjection has the following problems: the existing Salmonidae fish roe microinjection is mainly directly injected on a culture dish, the Salmonidae fish roe is non-sticky and the roe skin is hard, the roe is difficult to fix during injection, the operation difficulty is high, the injection efficiency is influenced, and the Salmonidae fish belongs to typical cold water fish, the temperature requirement of the roe in the hatching process is strict, the state of the Salmonidae fish roe is poor due to the temperature rise caused by long-time operation, the survival rate is reduced, and the experiment effect is seriously influenced. Therefore, there is a need for a salmonidae roe fixation device for microinjection that solves the above problems.
SUMMERY OF THE UTILITY MODEL
The present invention aims to provide a salmonidae roe fixing device for microinjection, which solves the problems of the prior art.
In order to achieve the purpose, the utility model provides the following scheme: the utility model provides a Salmonidae roe fixing device for microinjection, which comprises a base, wherein two sides of the top end of the base are fixedly connected with a supporting frame, the top end of the base is provided with a circular fixing groove which is positioned between the two support frames, the round fixing groove is internally provided with a agar layer, the top end of the base is provided with a limiting piece, the round fixing groove is detachably connected with the base through the limiting piece, the supporting frame is provided with an extrusion part which is positioned above the circular fixing groove and is arranged corresponding to the circular fixing groove, the supporting frame is fixedly connected with a motor, the motor is in transmission connection with the extrusion part, a constant temperature insulation board is arranged in the circular fixing groove, the circular fixing groove is provided with a temperature adjusting device, and the constant-temperature heat-insulation board is electrically connected with the temperature adjusting device.
Preferably, the extrusion portion is including rotating to be connected two-way threaded rod at carriage top, two-way threaded rod parallel arrangement just is located same horizontal plane, threaded connection has two screw thread sliders on the two-way threaded rod, screw thread slider bottom is provided with the extruded article, the extruded article with circular fixed slot corresponds the setting, be provided with the driving medium in the support frame, two-way threaded rod all with the driving medium transmission is connected, the driving medium with motor drive is connected.
Preferably, a cavity is formed in the support frame, the transmission part is connected to a worm in the cavity in a rotating mode, one end of the worm extends out of the cavity and is fixedly connected with an output shaft of the motor, one end, close to the worm, of the bidirectional threaded rod extends into a worm wheel fixedly connected in the cavity, and the worm wheel is meshed with the worm.
Preferably, the extruded part includes that one end articulates the connecting rod of screw thread slider bottom, the connecting rod other end articulates there is square frame, square frame with support frame sliding contact, be provided with circular stripper plate in the square frame, be provided with the connecting piece on the square frame, circular stripper plate passes through the connecting piece with the connection can be dismantled to the square frame.
Preferably, the connecting piece includes two splint that are located on same horizontal plane, the spout has all been seted up to the relative both sides of square frame inner wall, two splint all with spout sliding connection, two all seted up convex double-layered groove on the splint, two on the splint convex double-layered groove corresponds the setting, the one end of two springs of fixedly connected with on the square frame inside wall, two the other end of spring respectively with two splint fixed connection.
Preferably, the upper end and the lower end of the arc-shaped clamping groove are fixedly connected with arc-shaped supporting plates which are arranged in parallel.
Preferably, the locating part comprises a plurality of square blocks fixedly connected to the top end of the base, a plurality of square holes are formed in the bottom end of the circular fixing groove along the circumferential direction, and the square holes correspond to the square blocks
The utility model discloses the following technical effects: according to the Salmonidae roe fixing device for microinjection, the agar layer is arranged in the circular fixing groove, mechanical grooving is realized through the extrusion part, the labor force of operators is reduced, the grooving efficiency is improved, the stability of roes during microinjection is ensured through the arranged agar layer grooving, the phenomenon that the roes contact the circular fixing groove to cause overlarge local pressure is avoided, and the negative influence of environmental temperature on the roes is avoided through the arrangement of the constant-temperature heat insulation plate.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings needed to be used in the embodiments will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art to obtain other drawings without inventive exercise.
FIG. 1 is a schematic side view of a fixing device according to the present invention;
FIG. 2 is a schematic top view of the fixing device of the present invention;
FIG. 3 is a schematic view of the transmission member of the present invention;
FIG. 4 is a schematic view of a position limiting member according to the present invention;
wherein, 1, a base; 2. a support frame; 3. a circular fixing groove; 4. a motor; 5. a constant temperature insulation board; 6. a temperature adjusting device; 7. a bidirectional threaded rod; 8. a threaded slider; 9. a worm; 10. a worm gear; 11. a connecting rod; 12. a square frame; 13. a circular extrusion plate; 14. a splint; 15. a spring; 16. a circular arc-shaped support plate; 17. and (6) square blocks.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
In order to make the aforementioned objects, features and advantages of the present invention comprehensible, embodiments accompanied with figures are described in further detail below.
The utility model provides a Salmonidae roe fixing device for microinjection, which comprises a base 1, wherein two sides of the top end of the base 1 are fixedly connected with support frames 2, the top end of the base 1 is provided with a circular fixing groove 3, the circular fixing groove 3 is positioned between the two support frames 2, in order to ensure the stability of roes during microinjection, an agar layer is arranged in the circular fixing groove, a circular groove or a strip-shaped groove is arranged on the agar layer, the size of the groove is matched with that of the roes, the top end of the base 1 is provided with a limiting part, the circular fixing groove 3 is detachably connected with the base 1 through the limiting part, the support frames 2 are provided with an extrusion part, the extrusion part is positioned above the circular fixing groove 3 and is arranged corresponding to the circular fixing groove 3, the support frames 2 are fixedly connected with a motor 4, the motor 4 is in transmission connection with the extrusion part, in order to ensure the temperature of the roes in the circular fixing groove 3 to be constant during the microinjection process, avoid the negative effects that ambient temperature brought, be provided with constant temperature heated board 5 in the circular fixed slot 3, be provided with attemperator 6 on the circular fixed slot 3, constant temperature heated board 5 and attemperator 6 electric connection, the temperature of adjustment constant temperature heated board 5 maintains at 6-8 ℃, satisfies the invariable low temperature in the operation process, helps improving the survival rate of the embryo after the injection.
Further optimizing the scheme, in order to make things convenient for observing injection dosage and injection position under the microscope, make the operation more accurate, effective, circular fixed slot 3 is this transparent material of polymethyl methacrylate.
Further optimization scheme, in order to realize the upper and lower displacement of extruded article, extrusion portion is including rotating two-way threaded rod 7 of connection at 2 tops of support frame, 7 parallel arrangement of two-way threaded rod just are located same horizontal plane, threaded connection has two screw thread slider 8 on 7 two-way threaded rod, 8 bottoms of screw thread slider are provided with the extruded article, the extruded article corresponds the setting with circular fixed slot 3, be provided with the driving medium in the support frame 2, two-way threaded rod 7 all are connected with the driving medium transmission, the driving medium is connected with motor 4 transmission.
Further optimization scheme, in order to realize going on in step of two-way threaded rod 7, stability when guaranteeing the extruded article lift, seted up the cavity in the support frame 2, the driving medium is including rotating the worm 9 of connecting in the cavity, the output shaft fixed connection of cavity and motor 4 is stretched out to the one end of worm 9, and fixedly connected with worm wheel 10 in the cavity is stretched into to the one end that two-way threaded rod 7 is close to worm 9, and worm wheel 10 meshes with worm 9.
Further optimize the scheme, in order to realize carrying out extrusion to the agar layer that 3 portions of circular fixed slot were placed, the extruded article includes that one end articulates at the connecting rod 11 of 8 bottoms of screw thread slider, and the connecting rod 11 other end articulates there is square frame 12, square frame 12 and 2 sliding contact of support frame, is provided with circular stripper plate 13 in the square frame 12, is provided with the connecting piece on the square frame 12, and circular stripper plate 13 passes through the connecting piece and can dismantle with square frame 12 and be connected.
Further optimizing the scheme, in order to guarantee the stability of the fish eggs when microinjection is carried out on the fish eggs placed in the agar layer, the agar layer can be provided with a circular groove and a strip-shaped groove, the bottom end of the corresponding circular extrusion plate 13 is provided with a hemispherical bulge or a plurality of strip-shaped bulges which are arranged in parallel, the agar layer is extruded and grooved, the agar layer is guaranteed not to penetrate through the bottom of the groove, the surface contact between the fish eggs and the circular fixed groove 3 is avoided, the situation that the local part of the fish eggs is pressed too large is avoided, in order to guarantee the convenient installation and disassembly of the circular extrusion plate 13, the replacement of different types of circular extrusion plates 13 is convenient, the connecting piece comprises two clamping plates 14 which are positioned on the same horizontal plane, the opposite two sides of the inner wall of the square frame 12 are provided with sliding grooves, the two clamping plates 14 are both provided with arc-shaped clamping grooves, the two arc-shaped clamping grooves on the two clamping plates 14 are correspondingly arranged, and one end of two springs 15 is fixedly connected on the inner side wall of the square frame 12, the other ends of the two springs 15 are fixedly connected with the two clamping plates 14 respectively.
In order to further ensure the stability of the circular extrusion plate 13, the upper end and the lower end of the circular clamping groove are fixedly connected with circular arc support plates 16, and the two circular arc support plates 16 are arranged in parallel.
Further optimize the scheme, in order to guarantee the stability of circular fixed slot 3, the locating part includes a plurality of square pieces 17 of fixed connection on 1 top of base, and a plurality of quad slit have been seted up along circumference in 3 bottoms of circular fixed slot, and the quad slit corresponds the setting with square piece 17.
The utility model provides a Salmonidae roe fixing device for microinjection, when in use, an agar layer is laid in a circular fixing groove 3, the circular fixing groove 3 is inserted into a square block 17, a circular extrusion plate 13 is selected, two clamping plates 14 are manually controlled to slide in opposite directions, the circular extrusion plate 13 corresponds to an arc-shaped supporting plate 16 on the two clamping plates 14, the two clamping plates 14 are fixed by the elasticity of a spring 15, the circular extrusion plate 13 is fixed by controlling the rotation of a worm 9 through a motor 4, the worm 9 drives two worm wheels 10 to rotate, the two worm wheels 10 drive two bidirectional threaded rods 7 to rotate, two threaded sliders 8 on the bidirectional threaded rods 7 move in opposite directions to drive a hinged connecting rod 11 to move downwards, so that a square frame 12 is driven to slide downwards to complete batch grooving treatment of the agar layer, the motor 4 is controlled to rotate in opposite directions to realize the separation of the circular extrusion plate 13 and the agar layer, roe are placed in an extruded groove, and adjusting the temperature of the constant-temperature heat-insulation plate 5, and taking down the circular fixing groove 3 to complete microinjection operation of the fish eggs under a microscope.
According to the Salmonidae roe fixing device for microinjection, which is provided by the utility model, the agar layer is arranged in the circular fixing groove 3, the mechanical slotting is realized through the extrusion part, the labor force of operators is reduced, the slotting efficiency is improved, the stability of roes during microinjection is ensured through the arranged slotting of the agar layer, the overlarge local pressure caused by the contact of the roes with the circular fixing groove 3 is avoided, the negative influence of the environmental temperature on the roes is avoided through the arrangement of the constant-temperature heat-insulation board 5, the structure of the device is simple, the operation is convenient, and the roe microinjection efficiency is greatly improved.
In the description of the present invention, it is to be understood that the terms "longitudinal", "lateral", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", and the like, indicate orientations or positional relationships based on those shown in the drawings, are merely for convenience of description of the present invention, and do not indicate or imply that the referenced devices or elements must have a particular orientation, be constructed and operated in a particular orientation, and thus, are not to be construed as limiting the present invention.
The above-described embodiments are merely illustrative of the preferred embodiments of the present invention, and do not limit the scope of the present invention, and various modifications and improvements of the technical solutions of the present invention can be made by those skilled in the art without departing from the spirit of the present invention, and the technical solutions of the present invention are within the scope of the present invention defined by the claims.
Claims (7)
1. A Salmonidae roe fixing device for microinjection is characterized by comprising a base (1), wherein two sides of the top end of the base (1) are fixedly connected with support frames (2), a circular fixing groove (3) is formed in the top end of the base (1), the circular fixing groove (3) is positioned between the two support frames (2), an agar layer is arranged in the circular fixing groove (3), a limiting part is arranged at the top end of the base (1), the circular fixing groove (3) is detachably connected with the base (1) through the limiting part, an extrusion part is arranged on the support frame (2), the extrusion part is positioned above the circular fixing groove (3) and corresponds to the circular fixing groove (3), a motor (4) is fixedly connected onto the support frame (2), and the motor (4) is in transmission connection with the extrusion part, the round fixing groove (3) is internally provided with a constant-temperature insulation board (5), the round fixing groove (3) is provided with a temperature adjusting device (6), and the constant-temperature insulation board (5) is electrically connected with the temperature adjusting device (6).
2. A salmonidae egg fixation device for microinjection according to claim 1, wherein: extrusion portion is connected including rotating two-way threaded rod (7), two at support frame (2) top two-way threaded rod (7) parallel arrangement just is located same horizontal plane, threaded connection has two screw thread slider (8) on two-way threaded rod (7), screw thread slider (8) bottom is provided with the extruded article, the extruded article with circular fixed slot (3) correspond the setting, be provided with the driving medium in support frame (2), two-way threaded rod (7) all with the driving medium transmission is connected, the driving medium with motor (4) transmission is connected.
3. A salmonidae egg fixation device for microinjection according to claim 2, wherein: the cavity is arranged in the support frame (2), the transmission part is connected with a worm (9) in the cavity in a rotating mode, one end of the worm (9) extends out of the cavity and is fixedly connected with an output shaft of the motor (4), the two-way threaded rod (7) is close to one end of the worm (9) extends into a worm wheel (10) fixedly connected with in the cavity, and the worm wheel (10) is meshed with the worm (9).
4. A salmonidae egg fixation device for microinjection according to claim 2, wherein: the extruded article include that one end articulates connecting rod (11) in screw slider (8) bottom, connecting rod (11) other end articulates there is square frame (12), square frame (12) with support frame (2) sliding contact, be provided with circular stripper plate (13) in square frame (12), be provided with the connecting piece on square frame (12), circular stripper plate (13) pass through the connecting piece with square frame (12) can be dismantled the connection.
5. A Salmonidae egg securing device for microinjection according to claim 4, wherein: the connecting piece is including two splint (14) that are located same horizontal plane, the spout has all been seted up to the relative both sides of square frame (12) inner wall, two splint (14) all with spout sliding connection, two arc-shaped clamp groove has all been seted up on splint (14), two on splint (14) arc-shaped clamp groove corresponds the setting, the one end of two springs (15) of fixedly connected with on square frame (12) inside wall, two the other end of spring (15) respectively with two splint (14) fixed connection.
6. A Salmonidae egg securing device for microinjection according to claim 5, wherein: arc-shaped supporting plates (16) are fixedly connected to the upper end and the lower end of each arc-shaped clamping groove, and the two arc-shaped supporting plates (16) are arranged in parallel.
7. A salmonidae egg fixation device for microinjection according to claim 1, wherein: the locating part includes fixed connection in a plurality of square pieces (17) on base (1) top, a plurality of quad slit have been seted up along circumference in circular fixed slot (3) bottom, the quad slit with square piece (17) correspond the setting.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202220112269.9U CN216614683U (en) | 2022-01-17 | 2022-01-17 | Salmonidae roe fixing device for microinjection |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202220112269.9U CN216614683U (en) | 2022-01-17 | 2022-01-17 | Salmonidae roe fixing device for microinjection |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN216614683U true CN216614683U (en) | 2022-05-27 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202220112269.9U Active CN216614683U (en) | 2022-01-17 | 2022-01-17 | Salmonidae roe fixing device for microinjection |
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| Country | Link |
|---|---|
| CN (1) | CN216614683U (en) |
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2022
- 2022-01-17 CN CN202220112269.9U patent/CN216614683U/en active Active
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