CN208532756U - A kind of mammal body-cell neucleus transplanting reconstructed embryo fusing device - Google Patents
A kind of mammal body-cell neucleus transplanting reconstructed embryo fusing device Download PDFInfo
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- CN208532756U CN208532756U CN201820555089.1U CN201820555089U CN208532756U CN 208532756 U CN208532756 U CN 208532756U CN 201820555089 U CN201820555089 U CN 201820555089U CN 208532756 U CN208532756 U CN 208532756U
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Abstract
The utility model discloses a kind of mammal body-cell neucleus transplanting reconstructed embryo fusing devices, including fusion ware, two slot electrodes and several micro-cavities；Several micro-cavities are the blind hole set on fusion ware bottom surface, accommodate reconstructed embryo in blind hole, and the diameter of micro-cavity is slightly less than the diameter of reconstructed embryo；Several micro-cavities are that two rows are arranged, the micro-cavity of each row is sequentially arranged at intervals, reconstructed embryo can be fixed and be arranged to micro-cavity, and due to adjusting the tiny movement in other reconstructed embryo directions the offset in direction will not occur for the reconstructed embryo for having adjusted direction and position, and can once operate 20-30 reconstructed embryo；One first groove is respectively provided on fusion ware bottom surface and positioned at the two sides of micro-cavity, the opposite two sidewalls of fusion ware are respectively provided with two second grooves, the both ends of one first groove are respectively connected to one second groove, and then form a slot electrode, there is wire in slot electrode, wire does not need to be fixed with glue, and wire directly passes through slot electrode and is inserted into ware bottom and can fix.
Description
Technical field
The utility model relates to transplant reconstructed embryo fusion device technology field, and in particular to a kind of mammalian somatic cell core
Transplant reconstructed embryo fusing device.
Background technique
The body-cell neucleus transplanting of mammal, i.e. somatic cell clone are one of modern age biotechnology and life science
The important breakthrough having an epoch-marking significance, preparation of the technology in the genetic improvement, bioreactor of domestic animal, diseased n animal mould
The fields such as type, organ xenotransplantation all have broad application prospects.In the nuclear transplantation operation of animal, by donorcells
Nucleus import egg mother cell matter in process be known as merge, the fusion method generallyd use in animal cloning at present is electric Jie
The method for leading fusion.The electricity common equipment of mediated fusion is platinum needle-like electrode and two kinds of BTX integration slot at present.
Platinum needle-like electrode needs under inverted microscope, with micromanipulation arm coordination electrode, keeps direction of an electric field thin with body
The contact surface of two cell membranes is vertical in born of the same parents-egg mother cell reconstructed embryo, is only capable of 1 piece of reconstructed embryo of operation every time, use scope is small,
Operating time is long, high to degree of being skilled in technique requirement.
BTX integration slot is most common fusion device, can operate more pieces of reconstructed embryos every time, needs to be arranged manually
The adjustment of column direction, needed for fusion liquid it is more, need to frequently replace fusion liquid, and the minor fluctuations of liquid level can all lead to two
Reconstructed embryo among electrode moves.At more pieces of once-through operation, the reconstructed embryo for having adjusted direction and position can be due to adjustment
The tiny movement in other reconstructed embryo directions and the offset that direction occurs, it is necessary to re-operate, repetitive operation is mostly and working efficiency
It is low.
It can be seen that there is also certain defects for the prior art.
Utility model content
In view of this, in order to solve the problems in the prior art, the utility model proposes a kind of mammalian somatic cell cores
Transplant reconstructed embryo fusing device.
The utility model is solved the above problems by following technological means:
A kind of mammal body-cell neucleus transplanting reconstructed embryo fusing device, including fusion ware, two slot electrodes and several
Micro-cavity；
Several described micro-cavities are the blind hole set on fusion ware bottom surface, can accommodate reconstructed embryo in blind hole, and micro-cavity is straight
Diameter is slightly less than the diameter of reconstructed embryo；
Several described micro-cavities are that two rows are arranged, and the micro-cavity of each row is sequentially arranged at intervals；
It is respectively provided with one first groove on the fusion ware bottom surface and positioned at the two sides of micro-cavity, it is described to merge the opposite of ware
Two second grooves are respectively provided on two sidewalls, the both ends of one first groove are respectively connected to one second groove, and then form a slot electrode,
There is wire in slot electrode.
Preferably, the half of the wire of the fusion ware bottom surface is sunk into slot electrode, and half is exposed at outside slot electrode.
Preferably, the micro-cavity relative misalignment arrangement of two rows.
Preferably, the wire is in the type of falling Π.
Preferably, the fusion ware be 35mm capsule for diameter.
Compared with prior art, the beneficial effects of the utility model are as follows:
The fusion liquid that the utility model needs is less, reduces picking up for embryo and searches range, reduces repetitive operation, can be with one
More pieces of reconstructed embryos of secondary operation, improve the efficiency of mixing operation.
Detailed description of the invention
It, below will be to required in embodiment description in order to illustrate more clearly of the technical scheme in the embodiment of the utility model
Attached drawing to be used is briefly described, it should be apparent that, the accompanying drawings in the following description is only some realities of the utility model
Example is applied, it for those of ordinary skill in the art, without creative efforts, can also be according to these attached drawings
Obtain other attached drawings.
Fig. 1 is the top view of the utility model；
Fig. 2 is the shaft side figure of the utility model；
Fig. 3 is the bottom view of the utility model；
Fig. 4 is the structural schematic diagram of wire in the utility model；
Fig. 5 is the relative position relation figure of micro-cavity and micro-cavity in the utility model.
Description of symbols:
1, ware is merged；2, micro-cavity；3, slot electrode；4, wire.
Specific embodiment
To keep the above objects, features, and advantages of the utility model more obvious and easy to understand, below in conjunction with attached drawing and
The technical solution of the utility model is described in detail in specific embodiment.It should be pointed out that described embodiment is only
It is only the utility model a part of the embodiment, instead of all the embodiments, based on the embodiments of the present invention, this field
Those of ordinary skill's every other embodiment obtained without making creative work, belongs to the utility model
The range of protection.
It is to be appreciated that the orientation or positional relationship of the instructions such as term " top ", " bottom " is based on the figure
Orientation or positional relationship is merely for convenience of describing the present invention and simplifying the description, rather than the dress of indication or suggestion meaning
It sets or element must have a particular orientation, be constructed and operated in a specific orientation, therefore should not be understood as to the utility model
Limitation.
Term " first ", " second ", " third " are used for description purposes only, and are not understood to indicate or imply relatively important
Property or implicitly indicate the quantity of indicated technical characteristic.Define as a result, " first ", " second ", " third " feature can
To explicitly or implicitly include one or more of the features.In the description of the present invention, unless otherwise indicated,
" one group " is meant that two or more.
Embodiment
As shown in Figs. 1-5, a kind of mammal body-cell neucleus transplanting reconstructed embryo fusing device, including fusion 1, two electricity of ware
Pole slot 3 and several micro-cavities 2；
Several described micro-cavities 2 are the blind hole set on fusion 1 bottom surface of ware, can accommodate reconstructed embryo in blind hole, and micro-cavity 2
Diameter is slightly less than the diameter of reconstructed embryo；
Several described micro-cavities 2 are that two rows are arranged, and the micro-cavity 2 of each row is sequentially arranged at intervals, and the micro-cavity 2 can
With fixed and arrangement reconstructed embryo, and the reconstructed embryo for having adjusted direction and position will not be due to adjusting the thin of other reconstructed embryo directions
Little trick and the offset that direction occurs, and can once operate 20-30 reconstructed embryo；
One first groove, the phase of the fusion ware 1 are respectively provided on fusion 1 bottom surface of ware and positioned at the two sides of micro-cavity 2
Pair two sidewalls on be respectively provided with two second grooves, the both ends of one first groove are respectively connected to one second groove, so formed one electricity
Pole slot 3 has wire 4 in slot electrode 3, and wire 4 does not need to be fixed with glue, and wire 4 is directly inserted into ware by slot electrode 3
Bottom can be fixed, and wire 4 does not use glue to fix, and eliminate the negative effect of glue.
Preferably, the half of the wire 4 of fusion 1 bottom surface of ware is sunk into slot electrode 3, and half is exposed at outside slot electrode 3
Face.
Preferably, 2 relative misalignment of the micro-cavity arrangement of two rows.
Preferably, the wire 4 is in the type of falling Π.The both ends of the wire 4 extend to outside fusion ware 1, in order to
The connection of wire 4 and fusion instrument.
Preferably, it is 35mm capsule that the fusion ware 1, which is diameter,.Using 35mm capsule as fusion ware 1, integration slot is opposite
There was only one third in the integration slot of existing 90mm, merge liquid small volume, not only reduces the consumption of fusion liquid, and compared with
Small merges liquid product so that operating the moving area diminution of embryo, and then reduce picking up for embryo and search range, is conducive to merge
The efficient completion of operation.
Above-described embodiments merely represent several embodiments of the utility model, the description thereof is more specific and detailed,
But it should not be understood as limiting the scope of the patent of the utility model.It should be pointed out that for the common of this field
For technical staff, without departing from the concept of the premise utility, various modifications and improvements can be made, these all belong to
In the protection scope of the utility model.Therefore, the scope of protection shall be subject to the appended claims for the utility model patent.
Claims (5)
1. a kind of mammal body-cell neucleus transplanting reconstructed embryo fusing device, which is characterized in that including merging ware, two slot electrodes
With several micro-cavities；
Several described micro-cavities are the blind hole set on fusion ware bottom surface, reconstructed embryo can be accommodated in blind hole, and the diameter of micro-cavity omits
Less than the diameter of reconstructed embryo；
Several described micro-cavities are that two rows are arranged, and the micro-cavity of each row is sequentially arranged at intervals；
One first groove, the opposite two sides of the fusion ware are respectively provided on the fusion ware bottom surface and positioned at the two sides of micro-cavity
Two second grooves are respectively provided on wall, the both ends of one first groove are respectively connected to one second groove, and then form a slot electrode, electrode
There is wire in slot.
2. mammal body-cell neucleus transplanting reconstructed embryo fusing device according to claim 1, which is characterized in that described to melt
The half for closing the wire of ware bottom surface is sunk into slot electrode, and half is exposed at outside slot electrode.
3. mammal body-cell neucleus transplanting reconstructed embryo fusing device according to claim 1, which is characterized in that two rows
The micro-cavity relative misalignment arrangement.
4. mammal body-cell neucleus transplanting reconstructed embryo fusing device according to claim 1, which is characterized in that the gold
Belonging to silk is in the type of falling Π.
5. mammal body-cell neucleus transplanting reconstructed embryo fusing device according to claim 1-4, feature exist
In the fusion ware is that diameter is 35mm capsule.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820555089.1U CN208532756U (en) | 2018-04-18 | 2018-04-18 | A kind of mammal body-cell neucleus transplanting reconstructed embryo fusing device |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201820555089.1U CN208532756U (en) | 2018-04-18 | 2018-04-18 | A kind of mammal body-cell neucleus transplanting reconstructed embryo fusing device |
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| CN208532756U true CN208532756U (en) | 2019-02-22 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2022011729A1 (en) * | 2020-07-14 | 2022-01-20 | 苏州大学 | Multi-efficiency accurate electrical stimulation device and method for egg cell |
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2018
- 2018-04-18 CN CN201820555089.1U patent/CN208532756U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2022011729A1 (en) * | 2020-07-14 | 2022-01-20 | 苏州大学 | Multi-efficiency accurate electrical stimulation device and method for egg cell |
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Effective date of registration: 20230404 Address after: One of Shop 202, No. 16, Peixian East Road, Dongyong Town, Nansha District, Guangzhou City, Guangdong Province, 510000 Patentee after: Guangdong Zhongxin Seed Technology Co.,Ltd. Address before: 527400 9 East Causeway North Road, Xinxing County new town, Yunfu, Guangdong Patentee before: WENS FOODSTUFF GROUP Co.,Ltd. |
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| TR01 | Transfer of patent right |