CN206858588U - A kind of fusing device for nuclear transplantation - Google Patents
A kind of fusing device for nuclear transplantation Download PDFInfo
- Publication number
- CN206858588U CN206858588U CN201720726892.2U CN201720726892U CN206858588U CN 206858588 U CN206858588 U CN 206858588U CN 201720726892 U CN201720726892 U CN 201720726892U CN 206858588 U CN206858588 U CN 206858588U
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- China
- Prior art keywords
- adhesive tape
- glass adhesive
- chassis
- wire
- fusing device
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Abstract
It the utility model is related to nuclear transfer technology field, more particularly to a kind of fusing device for nuclear transplantation.It includes chassis and two wires being oppositely arranged, the both ends of the wire are individually fixed in the relative both sides in the chassis edge, the wire includes left section, stage casing and right section, and the left section of axial direction is socketed with left glass adhesive tape, and the right section of axial direction is socketed with right glass adhesive tape；The stage casing of two wires is respectively connected with an arc glass adhesive tape for being fixed on chassis and outwards arching upward, and the both ends of the both ends of the arc glass adhesive tape arc respectively with the corresponding stage casing are connected；The left glass adhesive tape, right glass adhesive tape and two arc glass adhesive tape are collectively forming the integration slot for holding fusion liquid.Fusion liquid used in the utility model is few, not only reduces the consumption of fusion liquid, and less fusion liquid make it that operating embryo's moving area reduces, and then reduces picking up for embryo and search scope, beneficial to the efficient completion of mixing operation.
Description
Technical field
Nuclear transfer technology field is the utility model is related to, more particularly to a kind of fusion for nuclear transplantation fills
Put.
Background technology
The body-cell neucleus transplanting of mammal, i.e. somatic cell clone are one of modern age biotechnology and life science
The important breakthrough having an epoch-marking significance, the body cell that it demonstrates differentiation can recover its undifferentiated state, and it is one to develop
Complete individual.The technology all succeeds in a variety of mammals, the system of genetic improvement, galactophore biological reactor in domestic animal
The inventions such as standby and human therapeutic clone all have broad application prospects.In the program of body-cell neucleus transplanting, body cell with
The fusion rate of egg mother cell is one of important step for influenceing nuclear transfer efficiency.Due to the small volume of body cell, its diameter is only
For 1/10 or so of egg mother cell, therefore the contact surface both when merging is smaller；And orientation is difficult to control so that fusion rate
It is low, generally 60% or so.The fusion device commonly used at present in nuclear transfer is two kinds of platinum needle-like electrode and BTX integration slots.
Platinum needle-like electrode is needed under inverted microscope, with micromanipulation arm coordination electrode, makes direction of an electric field thin with body
The contact surface of two cell membranes is vertical in born of the same parents-egg mother cell reconstructed embryo；In addition, it is only used for melting for oolemma reconstructed embryo
Close, and be only capable of operating 1 piece of reconstructed embryo every time；These factors cause that needle-like electrode use range is small, the operating time is long, technology is ripe
White silk degree requires high.
For BTX integration slots, its each operable more pieces of reconstructed embryo；And it can be used for having oolemma reconstructed embryo and lensless Fourier transform holography
Handmade cloning embryo, the orientation of body cell and egg mother cell can be relatively accurately controlled the latter by alternating current；And
To the former, then need to carry out the adjustment of orientation manually.However, BTX integration slots have the following disadvantages：
First, the fusion liquid needed for it is more, and the integration slot is fixed in a diameter of 90mm culture dish, to be completely covered
Integration slot, the fusion liquid operated every time need 40mL, and accumulation for harmful substance caused by avoiding shocking by electricity and bring into when moving ovum
Mineral oil pollution, it is necessary to frequently change fusion liquid, this result in merge liquid a large amount of consumption.
Second, because fusion liquid product is larger, the minor fluctuations of liquid level can all cause the shifting of the reconstructed embryo among two electrodes
It is dynamic, especially there is the reconstructed embryo of oolemma.If during more pieces of once-through operation, the reconstructed embryo for having adjusted direction and position can be due to
Adjust the tiny action in other reconstructed embryo directions and the skew in direction occur, it is necessary to re-operate, so cause the duplication of labour and
Operating efficiency reduces.
3rd, usually, in the fusion of more pieces of reconstructed embryos of progress, in advance more pieces of reconstructed embryos can be moved into integration slots
Row balance, but because fusion liquid product is big, it is also larger so as to the migration area of reconstructed embryo, cause to pick up the difficulty for searching operation on the contrary.
Utility model content
(1) technical problems to be solved
The technical problems to be solved in the utility model is to provide a kind of fusing device for nuclear transplantation, fusion dress
It is less to put the fusion liquid of needs, reduces picking up for embryo and searches scope, improve the efficiency of mixing operation.
(2) technical scheme
In order to solve the above-mentioned technical problem, the utility model provides a kind of fusing device for nuclear transplantation, its
Including chassis and two wires being oppositely arranged, the both ends of the wire are individually fixed in relative two in the chassis edge
Side, the wire include left section, stage casing and right section, and the left section of axial direction is socketed with left glass adhesive tape, and passes through the left glass
Glass adhesive tape is connected with tray bottom, and the right section of axial direction is socketed with right glass adhesive tape, and passes through the right glass adhesive tape and chassis
Bottom connects；
The stage casing of two wires is respectively connected with an arc glass adhesive tape for being fixed on chassis and outwards arching upward,
Both ends of the both ends of the arc glass adhesive tape arc respectively with the corresponding stage casing are connected；
It is collectively forming between the left glass adhesive tape, right glass adhesive tape and two arc glass adhesive tape for holding
Merge the integration slot of liquid.
Further, wherein one end of the wire extends to outside chassis, the connection for wire and fusion instrument.
Further, one end that the wire extends to outside chassis is bent in Q shapes, in order to wire and fusion instrument
Connection.
Further, two relative edges of the chassis are fixed in the both ends of the wire by glass cement respectively.
Further, the chassis is culture dish.Preferably, a diameter of 60mm or 90mm of the culture dish.
Further, the material of the wire is stainless steel.
Further, a diameter of 0.5~0.7mm of the wire, preferably 0.5mm；And/or the wire
Left section, stage casing and right section of length ratio are 2: 0.8~1.2: 2, preferably 2: 1: 2.
Further, in cross-sectional direction, the arc glass adhesive tape semicircular in shape；The cross-sectional direction refers to
The direction parallel with tray bottom.
Further, two wires are axisymmetricly set with a diameter of axle on the chassis.
Further, the two 0.5~1.0mm of wire interval, preferably 0.5mm.
(3) beneficial effect
Above-mentioned technical proposal of the present utility model has the advantages that：
Fusing device of the present utility model, left glass adhesive tape, right glass adhesive tape and two arc glass adhesive tape are enclosed jointly
Into the integration slot for holding fusion liquid, relative to existing integration slot, the fusion liquid used in integration slot of the present utility model
Product is smaller, not only reduces the consumption of fusion liquid, and less fusion liquid accumulates and the moving area of operation embryo is reduced,
And then reduce picking up for embryo and search scope, be advantageous to the efficient completion of mixing operation.
Fusing device of the present utility model, the stage casing of its wire are the electrode part for arranging reconstructed embryo, the part
Do not fixed with glue, be advantageous to the accurate of embryo and paste, eliminate the negative effect of glue.
Brief description of the drawings
Fig. 1 is the structural representation of fusing device described in the utility model embodiment；
Fig. 2 is the A-A sectional views in Fig. 1；
Fig. 3 is the top view of wire in chassis described in the utility model embodiment；
Wherein, 1, chassis；2nd, wire；3rd, left section；4th, stage casing；5th, right section；6th, left glass adhesive tape；7th, right glass adhesive tape；
8th, the first arc glass adhesive tape；9th, the second arc glass adhesive tape；10th, the first integration slot；11st, the second integration slot；12nd, the 3rd fusion
Groove；13rd, Q shapes are bent.
Embodiment
Embodiment of the present utility model is described in further detail with reference to the accompanying drawings and examples.Following examples
For illustrating the utility model, but can not be used for limiting the scope of the utility model.
In description of the present utility model, unless otherwise indicated, " more " are meant that two or more than two；Term
The orientation or position relationship of the instruction such as "left", "right", " interior ", " outer ", " bottom ", " top " be based on orientation shown in the drawings or
Position relationship, it is for only for ease of description the utility model and simplifies description, rather than indicates or imply signified device or member
Part must have specific orientation, with specific azimuth configuration and operation, therefore it is not intended that to limitation of the present utility model.
In addition, term " first ", " second ", " the 3rd " etc. are only used for describing purpose, and it is not intended that indicating or implying relatively important
Property.
, it is necessary to illustrate in description of the present utility model, unless otherwise clearly defined and limited, term " phase
Even ", " connection " should be interpreted broadly, for example, it may be being fixedly connected or being detachably connected, or be integrally connected；Can
To be to be joined directly together, can also be indirectly connected by intermediary.For the ordinary skill in the art, tool can be regarded
Body situation understands concrete meaning of the above-mentioned term in the utility model.
As shown in FIG. 1 to 3, a kind of fusing device for nuclear transplantation is present embodiments provided, it includes chassis 1
With two wires being oppositely arranged 2, the relative both sides in the edge of chassis 1 are fixed in the both ends of wire 2 by glass cement respectively.
Referring to Fig. 3, wire 2 includes left section 3, stage casing 4 and right section 5；Referring to Fig. 2 and Fig. 3, left section of 3 axial peripheries are socketed with left glass
Adhesive tape 6, and be connected by left glass adhesive tape 6 with the bottom of chassis 1, right section of 5 axial peripheries are socketed with right glass adhesive tape 7, and pass through
Right glass adhesive tape 7 is connected with the bottom of chassis 1；
Referring to Fig. 1 and Fig. 2, the stage casing 4 of wire 2 is connected with the first arc glass for being fixed on chassis 1 and outwards arching upward
Glass adhesive tape 8, the stage casing 4 of another wire 2 are connected with the second arc glass adhesive tape 9 for being fixed on chassis 1 and outwards arching upward, the
One arc glass adhesive tape 8 and the second arc glass adhesive tape 9 are axisymmetricly set, the first arc glass adhesive tape 8 and the second arc glass
Both ends of the both ends of the arc of glass adhesive tape 9 respectively with corresponding stage casing 4 are connected；
Two one metal wires 2 and its corresponding left glass adhesive tape 6, the and of 7 and first arc glass adhesive tape of right glass adhesive tape 8
The integration slot for holding fusion liquid is collectively forming between second arc glass adhesive tape 9, specifically：First arc glass adhesive tape 8
The first integration slot 10 is surrounded with the stage casing 4 of corresponding wire 2, two one metal wires 2 surround the second integration slot 11, the second arc glass
Glass adhesive tape 9 and corresponding stage casing 4 surround the 3rd integration slot 12.
In the present embodiment, chassis 1 is a diameter of 90mm culture dish.In cross-sectional direction, the first arc glass adhesive tape 8
With 9 equal semicircular in shape of the second arc glass adhesive tape.Wherein one end of wire 2 extends to outside chassis 1 in Q shapes bending 13, so as to
In the connection of wire 2 and fusion instrument.The material of wire 2 is stainless steel, a diameter of 0.5mm, left section 3, stage casing 4 and right section 5
Length ratio be 2：1：2.Two one metal wires 2 are axisymmetricly set with a diameter of axle on chassis 1, two one metal wire intervals
0.5mm.Left glass adhesive tape 6, right glass adhesive tape 7, height (the i.e. glue of the first arc glass adhesive tape 8 and the second arc glass adhesive tape 9
The distance between bar and culture dish bottom) it is equal.
The process that body-cell neucleus transplanting is carried out using above-mentioned fusing device is as follows：
(1) median size volume, the body cell for the smooth in appearance for recovering circle is selected to be used as donorcells, by egg mother cell
After handling 3~4s in 200 μ g/mL phytolectins (PHA) drops, it is put into one-to-one in the drop containing above-mentioned donorcells
Adhesion body cell；
(2) fusion liquid is full of the first integration slot 10, the second integration slot 11 and the 3rd integration slot 12；
(3) the more pieces of egg mother cells-body cell complex for intending operation is moved into the first integration slot 10, balances 2min；
(4) 3V alternating currents (AC) are imposed, multiple egg mother cells in the first integration slot 10-body cell complex are moved into the
In two integration slots 11, and it is arranged on the electrode of downside, direction is vertical with electrode；
(5) equally in the presence of AC field, another egg mother cell is adhered on above-mentioned complex, make its arrangement
Order is egg mother cell-body cell-egg mother cell, and ensures that its orientation is vertical with electrode；
(6) it is multiple to above-mentioned egg mother cell-body cell-egg mother cell that 50V/mm, 9 μ s, the direct current (DC) of 1 pulse are imposed
Zoarium carries out electro' asion；
(7) will be put into by egg mother cell-body cell-oocyte complex of mixing operation in the 3rd integration slot 12,
Fusing device is removed after balance 2min, detection fusion situation after 30min.
In summary, the fusing device of the present embodiment, two one metal wires and its corresponding left glass adhesive tape, right glass cement
Bar and the first arc glass adhesive tape and the second arc glass adhesive tape surround the integration slot for holding fusion liquid jointly, relatively
In existing integration slot, the fusion liquid small volume used in integration slot of the present utility model, the consumption of fusion liquid is not only reduced,
And the space of less fusion liquid product and separation to operate the area reduction that embryo arbitrarily moves, and then reduce embryo
Pick up and search scope, be advantageous to the efficient completion of mixing operation.
Nuclear transfer is carried out using this fusing device, the fusion rate of lensless Fourier transform holography reconstructed embryo can be made to be up to more than 95%
(95.24%)；Fusion liquid product used greatly reduces, as long as operation 3mL every time, is the 1/10 of existing equipment；Operate embryo
Moving area reduce, only integration slots 12 of first the second integration slots of integration slot 10+ 11+ the 3rd, rather than whole a diameter of
90mm culture dish, reduce picking up for embryo and search scope, accelerate the efficiency of mixing operation, reduce labor intensity；For arranging
The electrode part (i.e. wire stage casing) of row reconstructed embryo is not fixed with glue, is advantageous to the accurate of embryo and is sticked, improves fusion
Efficiency.
Embodiment of the present utility model in order to example and description for the sake of and provide, and be not exhaustively or will
The utility model is limited to disclosed form.Many modifications and variations are aobvious and easy for the ordinary skill in the art
See.Selection and description embodiment are to more preferably illustrate principle and practical application of the present utility model, and make this area
Those of ordinary skill it will be appreciated that the utility model so as to designing the various embodiments with various modifications suitable for special-purpose.
Claims (10)
- A kind of 1. fusing device for nuclear transplantation, it is characterised in that including chassis and two wires being oppositely arranged, The both ends of the wire are individually fixed in the relative both sides in the chassis edge, and the wire includes left section, stage casing and the right side Section, the left section of axial direction is socketed with left glass adhesive tape, and is connected by the left glass adhesive tape with tray bottom, the right section of axle To being socketed with right glass adhesive tape, and it is connected by the right glass adhesive tape with tray bottom；The stage casing of two wires is respectively connected with an arc glass adhesive tape for being fixed on chassis and outwards arching upward, described Both ends of the both ends of arc glass adhesive tape arc respectively with the corresponding stage casing are connected；It is collectively forming between the left glass adhesive tape, right glass adhesive tape and two arc glass adhesive tape for holding fusion The integration slot of liquid.
- 2. the fusing device according to claim 1 for nuclear transplantation, it is characterised in that the wire is wherein One end extends to outside chassis.
- 3. the fusing device according to claim 2 for nuclear transplantation, it is characterised in that the wire is to chassis One end of outer extension is bent in Q shapes.
- 4. the fusing device according to claim 1 for nuclear transplantation, it is characterised in that the both ends of the wire Two relative edges of the chassis are fixed on by glass cement respectively.
- 5. the fusing device according to claim 1 for nuclear transplantation, it is characterised in that the chassis is culture Ware.
- 6. the fusing device according to claim 1 for nuclear transplantation, it is characterised in that the material of the wire For stainless steel.
- 7. the fusing device according to claim 1 for nuclear transplantation, it is characterised in that the diameter of the wire For 0.5~0.7mm；And/or left section of the wire, stage casing and right section of length ratio are 2：0.8~1.2：2.
- 8. the fusing device according to claim 1 for nuclear transplantation, it is characterised in that in cross-sectional direction, The arc glass adhesive tape semicircular in shape.
- 9. the fusing device according to claim 1 for nuclear transplantation, it is characterised in that two wires with A diameter of axle on the chassis is axisymmetricly set.
- 10. the fusing device according to claim 1 for nuclear transplantation, it is characterised in that two wires It is spaced 0.5~1.0mm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720726892.2U CN206858588U (en) | 2017-06-21 | 2017-06-21 | A kind of fusing device for nuclear transplantation |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720726892.2U CN206858588U (en) | 2017-06-21 | 2017-06-21 | A kind of fusing device for nuclear transplantation |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN206858588U true CN206858588U (en) | 2018-01-09 |
Family
ID=60826446
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201720726892.2U Expired - Fee Related CN206858588U (en) | 2017-06-21 | 2017-06-21 | A kind of fusing device for nuclear transplantation |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN206858588U (en) |
-
2017
- 2017-06-21 CN CN201720726892.2U patent/CN206858588U/en not_active Expired - Fee Related
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20180109 Termination date: 20190621 |