CN207376051U - A kind of microinjection pipe - Google Patents

Abstract

The utility model discloses a kind of microinjection pipes, including tube rod and tube head, tube rod is hollow-core construction, the lower end of tube rod is provided with tube head, tube head is hollow cone structure, the lower end of tube head is provided with tip, tip includes nozzle and pointed section, nozzle includes oval inner wall and oval outer wall, and the lower end of oval outer wall is provided with pointed section, and fixing sleeve is equipped with baffle on tube head, baffle includes casing and catch, catch is arranged on the lower end of casing, offered on catch for tube head and tip by through hole, distance of the catch away from pointed section tip is 8 15 μm.The utility model novel in structural design, and it is simple and practical, it can solve the problems, such as that current microinjection pipe has no idea accurately to control injection depth.

Description

A kind of microinjection pipe

Technical field

The utility model belongs to RESEARCH ON CELL-BIOLOGY technical field, more particularly to a kind of microinjection pipe.

Background technology

Microinjection technique is that external source target gene is directly injected into the protokaryon of fertilized eggs using microinjection, is made Exogenous origin gene integrator is moved to the fertilized eggs that will be integrated with foreign gene in the genome of recipient cell by embryo transfer technology again The intrauterine growth of receptor is planted so as to obtain transgenic animals.It is preferably a kind of gene of current gene transfering efficiency Transfer method can be shifted directly with the foreign gene for not containing prokaryotic vector DNA fragmentation, and the length of foreign gene is unrestricted System, up to 100kb, experimental period is relatively short.

Application of the microinjection technique in the cell development research of plant, animal is research body physiological and external life Change process erects bridge, and specific molecular probe and derivative cytoplasm ingredient are imported active somatic cell, to study cell work( The regulatory mechanism of energy provides the new visual field, therefore in the operating process of microinjection, operation instrument especially injection-tube Quality, shape and internal diameter be an important factor for influencing embryo quality, to directly affect the success or failure of microinjection technique, it is and current Microinjection pipe be difficult control to the injection depth of cell, and then influence the effect of injection, thus it is necessary to design one kind can The microinjection pipe of the high quality of injection depth is controlled, so that microscopic insemination technology can be smoothly completed.

Utility model content

The purpose of this utility model is to provide a kind of microinjection pipes, it is intended to solve current microinjection pipe and have no idea The problem of accurate control injection depth.

To achieve the above object, the technical solution adopted in the utility model is：

A kind of microinjection pipe, including tube rod and tube head, tube rod is hollow-core construction, and the lower end of tube rod is provided with tube head, pipe Head is hollow cone structure, and the lower end of tube head is provided with tip, and tip includes nozzle and pointed section, and fixing sleeve is equipped with gear on tube head Plate, baffle include casing and catch, and catch is arranged on the lower end of casing, offered on catch for tube head and tip by it is logical Hole.Distance of the catch away from pointed section tip is 8-15 μm..

It is further preferred that the internal diameter of tube rod is 0.5-0.12mm, the outer diameter of tube rod is 0.15-0.25mm.

It is further preferred that nozzle includes oval inner wall and oval outer wall, the lower end of oval outer wall is provided with pointed section.

It is further preferred that the minor axis diameter of oval inner wall is 4-6 μm, the minor axis diameter of the ellipse outer wall is 6-7 μm.

It is further preferred that nozzle is an inclined-plane, bevel angle is 24-30 °.

It is further preferred that the length of pointed section is 2-4 μm.

It is further preferred that tube rod, tube head and tip are in line.

It is further preferred that tube rod, tube head and tip are made by transparent glass tube body one-pass molding.

It is further preferred that the material of catch is transparent material.

Compared to the prior art the utility model, the advantage is that：

The utility model provides a kind of microinjection pipe, novel in structural design, and simple and practical, can solve at present The problem of microinjection pipe has no idea accurately to control injection depth.The microinjection pipe has unique tube head and tip, energy It is easy to, enters into the cell hurtless measure, be conducive to the flowing of intracellular liquid, and egg cell will not be caused to damage Wound, simultaneously because tube rod, tube head and tip are all transparent, facilitates look at the mobility status of liquid, and by tube head Catch can accurately control the depth of injection, it is ensured that the depth of per injection is the same, prevent injection depth it is excessively shallow or Too deep situation, so that injection Job Operations are convenient, additionally it is possible to improve work efficiency and injection effect.

The additional aspect and advantage of the utility model will be set forth in part in the description, partly will be from following description In become apparent or recognized by the practice of the utility model.

Description of the drawings

Fig. 1 is a kind of structure diagram of microinjection pipe of the utility model.

Fig. 2 is the enlarged diagram of the tip of the utility model.

Fig. 3 is the enlarged diagram of the baffle of the utility model.

Wherein：1st, tube rod；2nd, tube head；3rd, tip；4th, nozzle；5th, pointed section；6th, baffle；7th, casing；8th, catch；9th, lead to Hole；10th, oval inner wall；11st, oval outer wall.

Specific embodiment

The exemplary embodiment of the disclosure is more fully described below with reference to accompanying drawings.Although the disclosure is shown in attached drawing Exemplary embodiment, it being understood, however, that may be realized in various forms the disclosure without should be by embodiments set forth here It is limited.On the contrary, these embodiments are provided to facilitate a more thoroughly understanding of the present invention, and can be by the scope of the present disclosure Completely it is communicated to those skilled in the art.

As shown in Figure 1 to Figure 3, a kind of microinjection pipe that the utility model embodiment provides, including tube rod 1 and tube head 2, Tube rod 1 is hollow-core construction, and the lower end of tube rod 1 is provided with tube head 2, and tube head 2 is hollow cone structure, and the lower end of tube head 2 is provided with Tip 3, tip 3 include nozzle 4 and pointed section 5, and fixing sleeve is equipped with baffle 6 on tube head 2, and baffle 6 includes casing 7 and catch 8, gear Piece 8 is arranged on the lower end of casing 7, offered on catch 8 for tube head 2 and tip 3 by through hole 9.Catch 8 is away from 5 point of pointed section The distance at end is 8-15 μm.

The microinjection pipe that the utility model embodiment provides, novel in structural design, and simple and practical, tube rod 1 and pipe First 2 to contain foreign gene, and can once carry multiple sperms can inject multiple egg mother cells, the unique circular cone of injecting adaptor 2 Structure is conducive to the smooth motion of inclusion foreign gene, and foreign gene can be input among egg mother cell by nozzle 4, Egg cell can be pierced through by pointed section 5 easily, hurtless measure and carry out microinjection, casing 7 is set on tube head 2, to incite somebody to action Catch 8 is fixed on tube head 2, and catch 8 is to determine the depth of injection, in injection process, is made when pointed section 5 is pierced into egg cell When catch 8 is close to egg mother cell wall, stop again toward starting to inject into piercing, the depth that so can be ensured that per injection is the same , the excessively shallow or too deep situation of injection depth is prevented, so that injection Job Operations are convenient, additionally it is possible to make work efficiency with And the success rate of microinjection technique improves.

The internal diameter of tube rod 1 is 0.5-0.12mm, and the outer diameter of tube rod 1 is 0.15-0.25mm.It is such to be designed with beneficial to liquid The flowing of substance.

Nozzle 4 includes oval inner wall 10 and oval outer wall 11, and the lower end of oval outer wall 11 is provided with pointed section 5.It is such It is designed with beneficial to egg cell is pierced through, so as to be conducive to the importing of foreign gene.

The minor axis diameter of oval inner wall 7 is 4-6 μm, and the minor axis diameter of oval outer wall 8 is 6-7 μm.Such shape and interior It footpath when in use just can be for a sperm by so that the success rate of microinjection technique improves.

Nozzle 2 is an inclined-plane, and bevel angle is 24-30 °.Such be designed with enters cell beneficial to the microinjection pipe Process is more steady, improves injection effect.

The length of pointed section 5 is 2-4 μm.It is thin that short and sharp keen being designed with of pointed section is beneficial to precisely undamaged puncture ovum Born of the same parents.

Tube rod 1, tube head 2 and tip 3 are in line.Such design can not only be beneficial to injection, and convenient for injection behaviour Observation when making.

Tube rod 1, tube head 2 and tip 3 are made by transparent glass tube body one-pass molding.It is such design convenient for observation by The flowing of liquid when smart, at the same in fertilization procedures convenient for it is clear, comprehensively control sperm, and microinjection pipe is once Shaping can be conducive to improve the rigidity of injection-tube entirety, extend the service life used.

The material of catch 6 is transparent material.The design of transparent catch not only contributes to the depth of control injection, while also not It can influence the operation to liquid object qualitative observation and to sperm in injection-tube.

In conclusion a kind of microinjection pipe that the utility model embodiment provides, novel in structural design, and also it is simple real With can solve the problems, such as that current microinjection pipe has no idea accurately to control injection depth.The microinjection pipe has uniqueness Tube head and tip, can be easy to, hurtless measure into intracellular, be conducive to the flowing of intracellular liquid, and will not Egg cell is caused to damage, simultaneously because tube rod, tube head and tip are all transparent, facilitates look at the mobility status of liquid, And the depth of injection can be accurately controlled by the catch on tube head, it is ensured that the depth of per injection is the same, is prevented The excessively shallow or too deep situation of injection depth, so that injection Job Operations are convenient, additionally it is possible to make work efficiency and injection effect It improves.

While there has been shown and described that the embodiment of the utility model, it will be understood by those skilled in the art that： In the case where not departing from the principle and objective of the utility model can these embodiments be carried out with a variety of variations, modification, replaced And modification, the scope of the utility model are limited by claim and its equivalent.

Claims (9)

1. a kind of microinjection pipe, which is characterized in that including tube rod (1) and tube head (2), the tube rod (1) be hollow-core construction, institute The lower end for stating tube rod (1) is provided with tube head (2), and the tube head (2) is hollow cone structure, and the lower end of the tube head (2) is set There is tip (3), tip (3) includes nozzle (4) and pointed section (5), and fixing sleeve is equipped with baffle (6), baffle (6) bag on tube head (2) Casing (7) and catch (8) are included, catch (8) is arranged on the lower end of casing (7), is offered on catch (8) for tube head (2) and tip (3) by through hole (9), distance of the catch (8) away from pointed section (5) tip be 8-15 μm.

2. a kind of microinjection pipe as described in claim 1, which is characterized in that the internal diameter of the tube rod (1) is 0.5- 0.12mm, the outer diameter of tube rod (1) is 0.15-0.25mm.

3. a kind of microinjection pipe as described in claim 1, which is characterized in that the nozzle (4) includes oval inner wall (10) With oval outer wall (11), the lower end of oval outer wall (11) is provided with pointed section (5).

4. a kind of microinjection pipe as claimed in claim 3, which is characterized in that the minor axis diameter of the ellipse inner wall (10) is 4-6 μm, the minor axis diameter of the ellipse outer wall (11) is 6-7 μm.

5. a kind of microinjection pipe as described in claim 1, which is characterized in that the nozzle (2) is an inclined-plane, bevel angle For 24-30 °.

6. a kind of microinjection pipe as described in claim 1, which is characterized in that the length of the pointed section (5) is 2-4 μm.

7. a kind of microinjection pipe as described in claim 1, which is characterized in that the tube rod (1), tube head (2) and tip (3) In line.

8. a kind of microinjection pipe as claimed in claim 7, which is characterized in that the tube rod (1), tube head (2) and tip (3) It is made by transparent glass tube body one-pass molding.

9. a kind of microinjection pipe as described in claim 1, which is characterized in that the material of the catch (8) is transparent material.