CN215209423U

CN215209423U - Double-narrow-part horn mouth ovum holding tube

Info

Publication number: CN215209423U
Application number: CN202121477739.3U
Authority: CN
Inventors: 吕祁峰
Original assignee: Ninth Peoples Hospital Shanghai Jiaotong University School of Medicine
Current assignee: Ninth Peoples Hospital Shanghai Jiaotong University School of Medicine
Priority date: 2021-06-30
Filing date: 2021-06-30
Publication date: 2021-12-17
Anticipated expiration: 2031-06-30

Abstract

The utility model discloses a double-narrow-part horn mouth ovum holding tube, which comprises a glass capillary tube body, a capillary tube drawing part and a suction port, wherein the capillary tube drawing part is provided with a first narrow part and a second narrow part at local positions, the first narrow part is close to one end of the suction port, and the second narrow part is positioned at the bending part of the capillary tube drawing part; the inner diameters of the first narrow part and the second narrow part are both smaller than 30 μm, the inner diameter of the suction port is larger than 50 μm, and the first narrow part and the suction port form a horn mouth shape facing to the port direction. The ovum holding tube enables the operation of micro suction and injection of the ovum to be stable and controllable, and improves the accuracy, the high efficiency and the simplicity of the operation.

Description

Double-narrow-part horn mouth ovum holding tube

Technical Field

The utility model relates to an ovum holding tube, in particular to an ovum holding tube with a horn mouth at two narrow parts.

Background

At present, the known ovum holding tube is often used for carrying out fine operations of holding and fixing the ovum of an animal under a microscope, such as intracytoplasmic sperm injection, nuclear transplantation and the like, so that the ovum of the animal, such as the ovum of a mouse, can be fertilized regularly for scientific research; and the research on human mitochondrial genetic diseases and the like can be carried out by nuclear transplantation cloning animals or nuclear substance replacement therapy. Among these high-precision tip biological microscopy techniques, the holding of the fallopian tube is a very critical technical tool.

However, the caliber of the open end of the conventional fallopian tube is small at present. The larger the caliber is, the more difficult the direction, the size and the conversion speed of the positive and negative pressure loaded on the pipe are to be controlled, so that the direction, the size and the conversion process of the flow speed of the liquid in the pipe are very sensitive and difficult to be stably controlled. For the holding operation of the mouse ovum, a technology which can suck part of the ovum into the holding ovum tube to elongate the ovum and is beneficial to reducing the disintegration of the ovum after the ovum puncture operation appears at present. This requires a large opening of the ovum holding tube, which often causes a certain probability of ovum breakage in actual micromanipulation because it is difficult to stably control the speed of holding the ovum by the ovum holding tube. Also making this fine technique a highly difficult operation technique.

The key reasons for this are: the larger the minimum caliber in the micro glass tube is, the more difficult it is to control the direction, magnitude and switching speed of the positive and negative pressure loaded thereon. In contrast, in a microscopic glass tube having a minimum tube inner diameter of more than 10 μm, such as a fallopian tube, it is necessary to control the liquid inlet and outlet speed during the holding by filling the minimum tube inner diameter with a high viscosity liquid such as mineral oil. The mouse bellmouthing oviduct disclosed at present has a narrow part, but high viscous liquid such as mineral oil cannot exist in the narrow part for control, because the sharp change shape of the narrow part can cause short and uncontrollable change of the flow rate of the mineral oil at the narrow part. That is, the existing bellmouthed ovum holding tube still has difficulty in controlling the holding speed of the ovum.

SUMMERY OF THE UTILITY MODEL

In order to overcome the problem that the existing horn mouth holds the fallopian tube and still is difficult to control the holding speed of the ovum, the utility model aims to provide a double-narrow horn mouth holds the fallopian tube, which is used for holding the fine operation of holding and fixing the ovum of an animal under a microscope, particularly holding and fixing the ovum when the ovum of a mouse is subjected to micro insemination or nuclear transplantation.

In order to achieve the above object, the present invention provides the following technical solutions:

a double-narrow-part horn mouth oviduct comprises a glass capillary tube body, a capillary narrowing part and a suction port, wherein the capillary narrowing part is provided with a first narrowing part and a second narrowing part which are arranged at local positions, the first narrowing part is close to one end of the suction port, and the second narrowing part is arranged at the bending part of the capillary narrowing part; the inner diameters of the first narrow part and the second narrow part are both smaller than 30 μm, the inner diameter of the suction port is larger than 50 μm, and the first narrow part and the suction port form a horn mouth shape facing to the port direction.

Furthermore, the first narrow part and the second narrow part are both in an arc structure with a convex middle part.

Furthermore, the first narrow part and the second narrow part are formed by hot melting of a local pipe wall by a micro heating body. Further, the second stenosis is filled with mineral oil in use.

Has the advantages that: the utility model provides a two narrow portion horn mouths hold the oviduct, the capillary that holds the oviduct draws thin portion and is equipped with two narrow portions of local position, and the internal diameter of narrow portion is less than the internal diameter of holding the port, and the narrow portion that is close to the port constitutes loudspeaker form with the port to keep away from the narrow portion of port and pack mineral oil, because the mineral oil through the stagnation nature that produces when narrow portion, has slowed down the too fast problem of holding speed, makes the operation stable controllable, has improved the precision of operation, has reduced the loss ratio of sample; and the narrow part close to the port blocks part of the ovum sucked from the port with large caliber, so that the length of the ovum in the sucking direction can be controlled. The manufacturing method of the fallopian tube of the utility model is simple, the raw materials are easy to obtain, and the manufacturing cost is low; meanwhile, the use method of the ovum holding tube is simple, and the working efficiency is improved, so that the ovum holding tube is suitable for the micro-suction and injection operation of the ovum.

Drawings

Fig. 1 is a schematic structural view of the fallopian tube of the present invention.

FIG. 2 is a sectional view of the fallopian tube of the present invention.

FIG. 3 is a diagram illustrating the usage status of the fallopian tube of the present invention.

In the figure: 1. a glass capillary tube body; 2. drawing the thin part by a capillary tube; 21. a first stricture portion; 22. a second stricture portion; 3. a suction port; 4. a microheating body; 5. mineral oil; 6. an ovum.

Detailed Description

The present invention has been described in detail with reference to the specific embodiments, but the present invention is only by way of example and is not limited to the specific embodiments described above. Any equivalent modifications and substitutions to the disclosed embodiments are within the scope of the present invention as those skilled in the art will recognize. Accordingly, variations and modifications in equivalents may be made without departing from the spirit and scope of the invention, which is intended to be covered by the following claims.

The utility model provides a two pars narrowly hold fallopian tube with a horn mouth, as shown in fig. 1, includes glass capillary tube body 1, capillary draw-down portion 2 and holds port 3, capillary draw-down portion 2 is equipped with local position's first stenosis portion 21 and second stenosis portion 22, the appearance of first stenosis portion 21 and second stenosis portion 22 is the bellied arc structure in middle part, first stenosis portion 21 and second stenosis portion 22 are formed by microheating body (4) to local pipe wall hot melt back.

The first narrowing portion 21 is located at one end of the suction port 3, and the second narrowing portion 22 is located at the bend of the capillary narrowed portion 2; the inner diameters of the first narrow part 21 and the second narrow part 22 are both less than 30 μm, the inner diameter of the suction port 3 is greater than 50 μm, and the first narrow part 21 and the suction port 3 form a bell mouth shape facing the port direction; said second stenosis 22 is in use filled with mineral oil (5).

The design principle of the fallopian tube is as follows:

the utility model provides a fallopian tube, which comprises a glass capillary tube body, a capillary tube thinning part and a suction and injection port, wherein the capillary tube thinning part is provided with two narrowing parts at local positions, and the narrowing part close to the port and the port form a horn mouth shape; the two narrow parts are formed by thickening the tube walls at two local positions of the capillary drawn part by a hot melting method to form local narrow channels; when the device is used, the narrow part far away from the port is always in a state of being filled with mineral oil, the holding speed is slowed down by using the resistance of the mineral oil when the mineral oil passes through the narrow part, the operation is stable and controllable, and the narrow part near the port blocks part of the eggs sucked from the port with large caliber, so that the growth of the eggs in the holding direction is controllable.

Claims

1. The utility model provides a two pars narrowly hold fallopian tube with a horn mouth, includes glass capillary body (1), capillary draws thin portion (2) and holds port (3) with inhaling, its characterized in that: the capillary narrowing part (2) is provided with a first narrowing part (21) and a second narrowing part (22) at local positions, the first narrowing part (21) is close to one end of the suction port (3), and the second narrowing part (22) is positioned at the bending part of the capillary narrowing part (2); the inner diameters of the first narrow part (21) and the second narrow part (22) are both smaller than 30 μm, the inner diameter of the suction port (3) is larger than 50 μm, and the first narrow part (21) and the suction port (3) form a horn mouth shape facing the port direction.

2. The double-stenosis-flaring fallopian tube of claim 1, wherein said first (21) and second (22) stenosis portions are each convexly curved in configuration.

3. The double-stenosis-flaring ovum-holding tube as claimed in claim 1, wherein the first stenosis portion (21) and the second stenosis portion (22) are formed by heat-melting a partial tube wall by a microheating body (4).

4. The double-stenosis-trumpet tubal of claim 1 wherein the second stenosis (22) is filled with mineral oil in use.