CN211005406U - Laboratory is with simple and easy device of enzyme reaction - Google Patents

Abstract

The utility model discloses a laboratory is with simple and easy device of enzyme reaction, including the glass bottle body, the glass bottle body comprises upper end bottleneck and the oval flat bottom bottle of lower extreme, has seted up the same glass slot of a plurality of structures on the bottle, and the interpolation is provided with enzyme liquid dropwise add pipe in the glass slot. The utility model discloses novel structure, reasonable in design, operation convenient to use can improve experimental efficiency greatly. The oval bottle body is more convenient to arrange with the glass slot; the upper end of the glass slot is wide, and the lower end of the glass slot is narrow, so that the enzyme solution dripping pipe can be conveniently inserted into the glass slot, can be fixed in the glass slot without leaking, and is vertical to the bottom of the bottle; the arrangement of the enzyme solution dripping pipe is convenient for accurate quantification.

Description

Laboratory is with simple and easy device of enzyme reaction

Technical Field

The utility model belongs to the technical field of experimental device, concretely relates to laboratory is with simple and easy device of enzyme reaction.

Background

Enzyme engineering refers to the process of converting a quantitative reaction substrate into a product within a quantitative time by utilizing the biocatalytic action of an enzyme through sufficient contact of the enzyme and the substrate in a reaction vessel under certain reaction conditions (temperature, pH, ionic strength, etc.). The success or failure of an enzyme reaction experiment is generally related to whether the reaction conditions are satisfactory, whether the reaction time is accurate, and whether the enzyme preparation and the substrate solution are accurately quantified. The following problems often occur in the operation of specific enzyme reaction experiments: firstly, because the enzymatic reaction is very rapid and is usually required to be carried out at a specific optimal action temperature, the enzymatic preparation and the substrate solution need to be preheated to the temperature in the water bath in advance respectively, so that the reaction can be carried out at the same temperature after the two are mixed. However, in a specific experiment, since the enzyme solution and the substrate solution are preheated in separate vessels, there is a possibility that the preheating temperatures of the two solutions may deviate due to operational problems. Secondly, the enzyme reaction needs to be finished within a certain time, and the reaction speed is high, so that the experimental result is greatly different due to the deviation of the reaction time, and the accurate timing is needed. When the enzyme reaction conditions are more, the reaction time is likely to be inaccurate when a liquid is removed by a pipette at ordinary times, the operation is relatively complex, and the repeatability of the experiment is poor. At present, enzyme reaction devices are generally large in size, complex in structure and high in cost, while laboratory enzyme reaction experiments are generally small in volume and are only carried out in a glass container such as a beaker or a test tube, and no relevant patent or literature reports that an enzyme preparation and a substrate solution are simultaneously integrated into one glass container to be preheated to complete the reaction.

SUMMERY OF THE UTILITY MODEL

To the problem that exists among the prior art, the utility model aims to provide a simple and easy device of enzyme reaction is used in laboratory that simple structure, can preheat the completion reaction in integrated a glass container simultaneously with enzyme preparation and substrate solution.

The utility model discloses a following technical scheme realizes:

the simple enzyme reaction device for the laboratory is characterized by comprising a glass bottle body, wherein the glass bottle body consists of an upper end bottleneck and a lower end oval flat-bottom bottle body, a plurality of glass slots with the same structure are formed in the bottle body, and an enzyme solution dropping pipe is inserted and connected in the glass slots.

The simple enzyme reaction device for the laboratory is characterized in that the bottleneck, the bottle body and the glass slot are arranged in an integrated mode.

The utility model provides a laboratory is with simple and easy device of enzyme reaction, its characterized in that the glass slot is the glass pipe that link up from top to bottom, outside the upper end of this glass slot stretched out the bottle, inside the lower extreme stretched into the bottle, and the diameter of glass slot is by last to reducing gradually down.

The simple enzyme reaction device for the laboratory is characterized in that the enzyme solution dropping pipe is a graduated pipe with a piston, and the lower end of the enzyme solution dropping pipe is reduced to form a sharp outlet.

The simple enzyme reaction device for the laboratory is characterized in that a pointed outlet at the lower end of the enzyme liquid dropping pipe is just clamped and arranged in a glass slot, and the clamping position is arranged at the position of a bottle body.

The simple enzyme reaction device for the laboratory is characterized in that the glass slot is vertical to the bottle body.

The utility model discloses novel structure, reasonable in design, operation convenient to use can improve experimental efficiency greatly. The oval bottle body is more convenient to arrange with the glass slot; the upper end of the glass slot is wide, and the lower end of the glass slot is narrow, so that the enzyme solution dripping pipe can be conveniently inserted into the glass slot, can be fixed in the glass slot without leaking, and is vertical to the bottom of the bottle; the arrangement of the enzyme solution dripping pipe is convenient for accurate quantification.

Drawings

FIG. 1 is a schematic view of the overall structure of the present invention;

in the figure, 1-bottle neck, 2-bottle body, 3-glass slot, 4-piston and 5-graduated tube.

Detailed Description

The present invention will be described in further detail with reference to the accompanying drawings, and specific embodiments will be given.

As shown in figure 1, the utility model relates to a laboratory is with simple and easy device of enzyme reaction, this simple and easy device structure is comparatively simple, easily realizes, and the practicality is stronger, and simultaneously, this simple and easy device still can realize that enzymic preparation and substrate solution heat simultaneously, need not to move after the heating and gets, and direct reaction ensures that reaction time is accurate. Specifically including the glass bottle body, the glass bottle body comprises upper end bottleneck and the oval flat bottom bottle of lower extreme, sets up the glass slot of the perpendicular same with the bottle of a plurality of structures on the bottle, and bottleneck, bottle, glass slot formula as an organic whole set up, and the glass pipe that the glass slot is for lining up from top to bottom, and outside this glass pipe's the upper end stretched out the bottle, the lower extreme stretched into inside the bottle, and the diameter of glass slot was reduced by last to bottom gradually.

In order to realize the dropwise addition of the enzyme liquid, an enzyme liquid dropwise adding pipe is inserted and connected in the glass slot, the enzyme liquid dropwise adding pipe is a scale pipe with a piston, the lower end of the scale pipe is reduced to form a sharp outlet, the sharp outlet at the lower end of the enzyme liquid dropwise adding pipe is just clamped and connected in the glass slot, and the clamping position is at the position of the bottle body. The piston is in close contact with the graduated tube and can reciprocate in the graduated tube under the action of external force, and volume scales are displayed on the graduated tube.

When the enzyme reaction experiment is carried out, a certain volume of substrate solution can be placed in the bottle in advance, the enzyme solution dripping pipe and the connected piston are drawn out from the glass slot, the excessive enzyme solution is sucked by pulling the piston, and then the graduated tube and the connected piston are placed in the glass slot. The reaction device is placed into a water tank with a certain temperature, and the water in the water tank is preferably used for immersing the substrate solution and the enzyme solution, but cannot exceed the upper opening of the glass slot so as to fully preheat the substrate solution and the enzyme solution simultaneously. After the preheating is finished and the reaction temperature is reached, the piston is pressed forcibly, so that the enzyme liquid in the graduated tube is injected into the bottle body, and the specific numerical value can be accurately determined through the graduation due to the fact that the graduated tube is inserted vertically. When the enzyme solution is brought into contact with the substrate solution, the enzyme reaction starts rapidly. When the reaction time is up and the reaction needs to be stopped, the stopping liquid is injected into the bottle body. Therefore, the enzyme solution and the substrate solution can reach the reaction temperature at the same time, the accuracy of the reaction time can be ensured, and the accuracy and the repeatability of the enzyme reaction result can be ensured as much as possible.

Claims (6)

1. The utility model provides a laboratory is with simple and easy device of enzyme reaction, its characterized in that includes the glass bottle body, the glass bottle body comprises upper end bottleneck (1) and the oval flat bottom bottle of lower extreme (2), a plurality of glass slots (3) that the structure is the same have been seted up on bottle (2), the interpolation is provided with enzyme liquid dropwise add pipe in glass slot (3).

2. The laboratory enzyme reaction simple device according to claim 1, wherein the bottle neck (1), the bottle body (2) and the glass slot (3) are integrally formed.

3. The laboratory enzyme reaction simple device according to claim 1, wherein the glass insertion groove (3) is a glass tube which is through from top to bottom, the upper end of the glass insertion groove (3) extends out of the bottle body (2), the lower end of the glass insertion groove (3) extends into the bottle body (2), and the diameter of the glass insertion groove (3) is gradually reduced from top to bottom.

4. The laboratory enzyme reaction simple device according to claim 1, wherein the enzyme solution dropping pipe is a graduated tube (5) with a piston (4), and the lower end of the graduated tube is reduced to form a sharp outlet.

5. The laboratory enzyme reaction simple device according to claim 4, wherein the pointed outlet at the lower end of the enzyme solution dropping pipe is just clamped and arranged in the glass slot (3), and the clamping position is at the bottle body (2).

6. The laboratory enzyme reaction facile device of claim 1, wherein the glass insert groove (3) is disposed vertically to the bottle body (2).

Images