CN218618068U - PCR detect reagent box apron - Google Patents

Abstract

The utility model discloses a PCR detect reagent box apron, a serial communication port, including the pellosil, one side of pellosil is arranged and is had a plurality of annular stopcock, annular stopcock with pellosil integrated into one piece. The PCR reagent kit cover plate made of the annular tube plug and the silica gel film can reduce the consumption of materials and the production cost on one hand, and on the other hand, when a sample is made, the injection needle can inject the reagent by penetrating through the silica gel film.

Description

PCR detect reagent box apron

Technical Field

The utility model relates to a kit apron field, concretely relates to PCR detect reagent box apron.

Background

Biological detection technology is increasingly developed, the experimental process is increasingly complex, the requirements on experimental equipment are gradually improved, and the traditional biological detection instrument with single function cannot meet the current requirements, so that a biological detection automation workstation with the characteristics of high precision, high flux, high speed and the like becomes one of the necessary instruments for biochemical research, clinical diagnosis and other researches. The automatic biological detection workstation can replace manual operation to perform full-automatic biological detection, so that labor and cost are saved, the working efficiency is improved, and subjective errors are reduced.

The PCR detection technology is one of the most common biological detection methods in laboratories at present, and has high sensitivity. PCR, i.e., polymerase chain reaction, is a process of in vitro replication of daughter strand DNA complementary to a parent strand template DNA by denaturing, annealing, extending, etc., using the parent strand DNA as a template and a specific primer as an extension origin, under the catalysis of DNA polymerase. At present, the PCR detection technology is mature at home and abroad, all relevant standards are complete, the cost of the instrument and reagents is low, and other molecular biological experiments can be carried out after the PCR product is recovered. Although the existing PCR technology has been developed to a brand-new level, the problems of cross contamination, poor detection effect, poor experimental repeatability and the like caused by the phenomena of poor closure, leakage, reagent volatilization and the like in the sample preparation process still exist, so that the speed and the accuracy of PCR detection are directly influenced.

PCR reagent box cover plate's material, the structure and with the cooperation of kit directly influence detection speed, the accuracy of testing result and result, at present, mainstream PCR cover plate includes aluminium membrane apron in the market, transparent plastic film apron and silicon rubber apron, aluminium membrane apron need cooperate with the heat-sealing machine usually when using, higher heat-seal temperature can improve the ambient temperature of reagent, can cause uncertain influence to the detection of reagent, secondly, because the aluminium membrane is opaque, hardly in time observe the reaction condition of reagent during the detection, there is hysteresis quality and influence uncertainty in the detection, in addition, when the aluminium membrane uses as the apron, can only use once, not only extravagant resource has also greatly influenced actual detection efficiency. The transparent plastic film cover plate is usually matched with an adhesive, so that the disassembly is difficult, the adhesive is easy to remain on the kit after the disassembly, and the plastic film cover plate cannot be reused. The silicon rubber cover plate on the market has high transparency, does not need heat sealing or bonding by using an adhesive, can be used for multiple times, is not influenced by environment and reagents, and has high cost. In addition, in the process of manufacturing a PCR sample, reagents or RNA (ribonucleic acid) need to be repeatedly dripped into a test tube of the kit for many times, when an aluminum film cover plate and a transparent plastic film cover plate are used, the existing cover plate is usually required to be torn off and then a new cover plate is heat-sealed or attached again, when the existing silicon rubber cover plate is used, the existing silicon rubber cover plate needs to be covered and uncovered for many times, and cross contamination is easily caused by many times of operation, so that the experimental result is influenced. Therefore, it is important to develop a PCR kit cover plate with high transparency, which can be tightly fitted with the kit and does not need to be repeatedly removed and covered.

Disclosure of Invention

An object of the utility model is to overcome the defect that prior art exists, provide a PCR detect reagent box apron to solve current PCR apron because of having nontransparent, not inseparable and need repeatedly take off when the preparation sample with the kit cooperation and cover and close sample pollution, detection efficiency low and the inaccurate scheduling problem of testing result that defects such as apron lead to.

In order to realize the above purpose, the utility model provides a PCR detect reagent box apron, a serial communication port, including the pellosil, one side of pellosil is arranged and is had a plurality of annular stopcock, annular stopcock with pellosil integrated into one piece.

In order to solve current PCR reagent box apron opaque and with the untight problem of kit cooperation, the utility model provides a PCR detect reagent box apron, this apron adopts transparent silica gel preparation, and the preferred solvent resistance that uses is good, the wearability is excellent, high temperature resistant and the high fluorine silica gel preparation of transparency, uses transparent silica gel preparation kit apron, when sample preparation even carries out the sample detection, can observe the condition in each test tube in the reagent box at any time, have the problem to handle in time, greatly improved the efficiency that reagent detected. The utility model provides an apron includes the silica gel membrane and sets up the annular stopcock in silica gel membrane one side, and the quantity and the internal diameter of size according to the quantity of test tube and test tube in the reagent box of annular stopcock are confirmed. The shape of the pipe plug is set to be annular instead of circular or boss shape, on one hand, the consumption of materials can be reduced, the production cost is reduced, on the other hand, when a sample is manufactured, the injection needle can penetrate through the silica gel film to inject the reagent, since the silica gel has self-repairability, after the injection needle is pulled out, the needle hole can be quickly repaired, so that an operator does not need to repeatedly uncover and cover the cover plate when the sample is manufactured, the possibility of cross contamination of the reagent in the test tube is reduced, and the accuracy of a detection result is improved.

According to a further preferred technical scheme, the silicon membrane is provided with a plurality of notches, and the notches are arranged inside the annular pipe plug.

Further preferred technical scheme is, the incision includes any one of a straight shape, a cross shape, a Y shape and a rice shape.

In order to make the test tube of inserting that the syringe needle can be better, the utility model discloses among the preferred technical scheme, still be provided with the incision on the pellosil, the incision sets up in the inside of annular stopcock, preferably sets up at annular center. The shape of the notch can be arranged in a plurality of shapes, common shapes of the notch comprise a straight shape, a cross shape, a Y shape and a rice shape, and besides the common shapes, the notch can also be arranged in other shapes, such as a herringbone shape, a snowflake shape and the like.

The further preferred technical scheme is that the outer diameter of the upper end of the annular pipe plug is larger than that of the lower end of the annular pipe plug. In order to make better the cooperation with the test tube of annular stopcock ability, it is preferred, the external diameter of annular stopcock upper end is greater than the external diameter of lower extreme, the external diameter of stopper mouth department is less than the external diameter of keeping away from stopper mouth department promptly, specifically, the external diameter of annular stopcock upper end is less than the internal diameter of test tube, the external diameter of lower extreme is less than or equal to the internal diameter of test tube, in the actual operation in-process, can be more convenient fill in the test tube with annular stopcock lower extreme, the upper end block is in test tube mouth department, annular stopcock and kit can inseparabler cooperation, reduce the emergence of weeping phenomenon.

According to a further preferable technical scheme, the silicon membrane is provided with a bulge, the bulge and the annular pipe plug are arranged on the same side of the silicon membrane, and the bulge is arranged on the periphery of the annular pipe plug.

Further preferably, the shape of the protrusion includes any one of a cylindrical shape, a spherical shape, a conical shape, a pyramidal shape, a prismatic shape, and a columnar body having a cross-shaped or a m-shaped cross section.

In order to make annular plug even apron and kit can inseparabler cooperation, avoid leading to actual pollution because of the apron drops at the in-process of taking the kit, further preferred technical scheme is last still to be provided with the protrusion, and the protrusion sets up around annular stopcock, and after inserting the annular stopcock into the test tube of kit, the protrusion block can effectively prevent droing of stopcock and apron in the periphery of test tube. The protrusions may be of various shapes, preferably including one of a cylindrical shape, a spherical shape, a conical shape, a pyramidal shape, a prismatic shape, a columnar body having a cross-shaped or a m-shaped cross-section, and may be of other shapes than those listed above.

Further preferably, the number of the annular pipe plugs is 96. The number of annular stopcock mainly depends on the quantity of test tube in the reagent box with it matched with, and the PCR kit of most on the market is 96 hole reagent boxes at present, can be in order to hold the reagent box of 96 test tubes, the utility model discloses in, the number of annular stopcock is preferred 96 on the apron.

The utility model has the advantages and the beneficial effects that:

1. the PCR reagent kit cover plate made of the annular tube plug and the silica gel film can reduce the consumption of materials and the production cost on the one hand, and on the other hand, when a sample is made, the injection needle can inject the reagent by penetrating the silica gel film.

2. The incision setting on the silica gel membrane can make the syringe needle insert the test tube more easily, and the back is extracted to the syringe needle, and the incision healing further prevents the pollution of reagent in the reagent box.

3. Insert the in vitro back of kit with annular stopcock, but the bellying block that annular stopcock periphery set up effectively prevents droing of stopcock and apron in the periphery of test tube.

Drawings

FIG. 1 is a schematic diagram of the structure of the cover plate of the PCR detection kit in the embodiment.

FIG. 2 is a side view of the cover plate of the PCR detecting kit in the embodiment.

In the figure: 1. a silica gel film; 2. an annular plug; 3. cutting; 4. and (4) a bulge.

Detailed Description

The following description will further describe embodiments of the present invention with reference to the accompanying drawings and examples. The following examples are only for illustrating the technical solutions of the present invention more clearly, and the protection scope of the present invention is not limited thereby.

Example 1

As shown in fig. 1, a PCR detection kit cover plate comprises a silicone membrane 1, 96 annular tube plugs 2 are arranged on one side of the silicone membrane 1, and the annular tube plugs 2 and the silicone membrane 1 are integrally formed. 96 notches 3 are formed in the silicon membrane 1, the notches 3 are cross-shaped, and the notches 3 are arranged in the annular center inside the annular pipe plug 2. The outer diameter of the upper end of the annular pipe plug 3 is larger than that of the lower end. The periphery of the annular pipe plug of the silicon membrane 1 is also provided with a bulge 4, and the bulge 4 is a columnar body with a cross-shaped section.

The above description is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, many modifications and decorations can be made without departing from the technical principle of the present invention, and these modifications and decorations should also be regarded as the protection scope of the present invention.

Claims (7)

1. The utility model provides a PCR detect reagent box apron, its characterized in that, includes the pellosil, a plurality of annular stopcock is arranged to one side of pellosil, annular stopcock with pellosil integrated into one piece.

2. The cover plate for a PCR detection kit according to claim 1, wherein the silicone membrane has a plurality of notches formed therein, and the notches are disposed inside the annular plug.

3. The cover plate for PCR detection kit of claim 2, wherein the notch comprises any one of a straight line shape, a cross shape, a Y shape and a rice shape.

4. The cover plate for PCR detection kit according to claim 1, wherein the outer diameter of the upper end of the annular plug is larger than that of the lower end.

5. The cover plate for a PCR detection kit according to claim 1, wherein the silicone membrane is provided with a protrusion, the protrusion and the annular tube plug are arranged on the same side of the silicone membrane, and the protrusion is arranged on the periphery of the annular tube plug.

6. The cover plate for PCR detection kit of claim 5, wherein the shape of the protrusions comprises any one of a cylinder, a sphere, a cone, a pyramid, a prism, and a column with a cross-shaped or a rice-shaped cross section.

7. The cover plate for PCR detection kit of claim 6, wherein the number of the ring-shaped tube plugs is 96.

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