CN215924962U

CN215924962U - Pulse type cell hypoxia high-pressure culture device

Info

Publication number: CN215924962U
Application number: CN202120374278.0U
Authority: CN
Inventors: 郭衍俊; 夏海龙; 何雪永
Original assignee: Shanghai Tow Int Co ltd
Current assignee: Shanghai Tow Int Co ltd
Priority date: 2021-02-10
Filing date: 2021-02-10
Publication date: 2022-03-01
Anticipated expiration: 2031-02-10

Abstract

The utility model relates to the technical field of cell culture devices, and discloses a pulse type cell hypoxia high-pressure culture device which comprises an incubator, wherein a water tray is fixedly arranged on the inner bottom wall of the incubator, a supporting plate is fixedly arranged on the inner side wall of the incubator, and a cell culture dish is arranged at the top of the supporting plate; the inner top wall of the incubator is fixedly provided with a fan and a sensor group, and the outer wall of the incubator is fixedly provided with an oxygen tube, a nitrogen tube and a carbon dioxide tube. The method provides personalized culture conditions by regulating parameters such as oxygen, pressure, temperature, carbon dioxide concentration and the like, and enables cells to be stably cultured and maintain the characteristics of the cells in vivo by simulating the microenvironment of the cells in vivo, so that the cells and organoids show better in vivo correlation in subsequent research. The utility model has novel design and the advantages of providing periodic pressure intervention, accurately regulating and controlling the microenvironment for cell growth and comprehensively simulating the physiological environment of different parts in vivo.

Description

Pulse type cell hypoxia high-pressure culture device

Technical Field

The utility model relates to the technical field of cell culture devices, in particular to a pulse type cell hypoxia high-pressure culture device.

Background

Long-term studies have shown that: cells in various tissue parts of the human body are generally in a hypoxic and hyperbaric microenvironment. Meanwhile, under the regulation and control of different oxygen and pressure, the cells can change in metabolism, gene and protein levels to different degrees, thereby affecting the appearance of the cells in form and behavior. Regulation of the microenvironment is an effective means of controlling cells.

For example, when a wound is formed on the skin surface, blood will flow out because the pressure in the human body is greater than atmospheric pressure, and because of this, it is known that cells in the human body are subjected to the pressure all the time, and when the cells leave the human body, the original pressure disappears, and the original pressure disappears together with the pressure-sensitive factors in the cells, thereby affecting the expression of certain proteins in the cells.

The pressure environment is an important factor for the in vitro proliferation of cells, and stem cells are affected by various pressures after being transplanted into a body. The mechanical environment in vivo is complex, and the biological effects of cells and various organelles under the action of pressure and the mechano-biochemical signal transduction pathways are all available. Researchers at home and abroad conduct mechanical stress intervention research on stem cells cultured in vitro, and find that a series of biological characteristics of the stem cells, such as proliferation and differentiation, can be changed correspondingly under a certain mechanical environment.

In vitro experiments, the response of cells to mechanical stimuli can be observed by designing specific loading patterns, controlling various variables (different types of force, magnitude, frequency, time, different strains of cells, etc.).

However, the conventional cell culture box cannot regulate the pressure to which the cells are subjected, and cannot accurately simulate the microenvironment of the cells in the body, so that organoid cells derived from the primary tumor cannot normally grow and exhibit the physiological activity in the body in subsequent experiments, which is very disadvantageous to the cell culture.

Based on this, we propose a pulsed low-oxygen high-pressure cell culture device.

SUMMERY OF THE UTILITY MODEL

Technical problem to be solved

Aiming at the defects of the prior art, the utility model provides the pulse type cell hypoxia high-pressure culture device which has the advantages of providing periodic pressure intervention, accurately regulating and controlling the microenvironment for cell growth and comprehensively simulating physiological environments of different parts in vivo.

(II) technical scheme

In order to realize the purposes of providing periodic pressure intervention, accurately regulating and controlling the microenvironment for cell growth and comprehensively simulating the physiological environments of different parts in vivo, the utility model provides the following technical scheme: a pulse type cell hypoxia high-pressure culture device comprises a culture box, wherein a water disc is fixedly arranged on the inner bottom wall of the culture box, a supporting plate is fixedly arranged on the inner side wall of the culture box, and a cell culture dish is arranged at the top of the supporting plate;

the air-conditioning system is characterized in that a fan and a sensor group are fixedly arranged on the inner top wall of the incubator, an oxygen pipe, a nitrogen pipe and a carbon dioxide pipe are fixedly arranged on the outer wall of the incubator, one ends of the oxygen pipe, the nitrogen pipe and the carbon dioxide pipe are communicated with the inside of the incubator, and the other ends of the oxygen pipe, the nitrogen pipe and the carbon dioxide pipe are respectively and fixedly connected with an oxygen tank, a nitrogen tank and a carbon dioxide tank;

the outer wall of incubator still through connection has communicating pipe, the terminal fixedly connected with pulse pressure generator of communicating pipe, pulse pressure generator is used for the air of periodically impressing through communicating pipe to the incubator, makes the incubator in produce the pressure and vibrate.

As a preferred technical scheme of the utility model, the incubator comprises an outer shell and an inner shell, and heating wires are uniformly arranged in a gap between the outer shell and the inner shell.

As a preferred technical solution of the present invention, the sensor group includes a temperature sensor, a humidity sensor, an oxygen concentration sensor, a carbon dioxide concentration sensor, and a pressure sensor.

As a preferable technical scheme of the utility model, the oxygen pipe, the nitrogen pipe and the carbon dioxide pipe are internally provided with a filter and an electromagnetic valve.

As a preferred technical scheme of the utility model, the outer wall of the incubator is also fixedly provided with an air leakage pipe, the air leakage pipe is communicated with the inside of the incubator, and the inside of the air leakage pipe is provided with an air leakage valve.

As a preferable technical scheme of the utility model, the pulse pressure generator comprises a seal box, an electric push rod and a piston, the electric push rod is fixedly arranged at the top of the seal box, the piston is fixedly arranged at the top of an output shaft of the electric push rod, the piston is movably arranged in the seal box, and the seal box is communicated with the incubator through a communicating pipe.

As a preferred technical scheme of the utility model, the incubator further comprises a touch screen and a single chip microcomputer, wherein the touch screen is fixedly arranged on the outer wall of the incubator.

As a preferred technical scheme of the present invention, the output ends of the sensor group and the touch screen are electrically connected to the input end of the single chip, and the output end of the single chip is electrically connected to the input ends of the heating wire, the electromagnetic valve, the air release valve and the electric push rod respectively.

(III) advantageous effects

Compared with the prior art, the utility model provides an impulse type cell hypoxia high-pressure culture device, which has the following beneficial effects:

1. the pulse type cell hypoxia high-pressure culture device provides personalized culture conditions by regulating and controlling parameters such as oxygen, pressure, temperature and carbon dioxide concentration and combining with a culture medium meeting physiological needs, enables cells to be stably cultured by simulating a microenvironment of the cells in vivo, and keeps the characteristics of the cells in vivo, so that the cells and organoids show better in vivo correlation in subsequent research.

2. According to the pulse type cell hypoxia high-pressure culture device, the output shaft of the electric push rod is controlled by the single chip microcomputer to extend out or retract, the output shaft of the electric push rod extends out or retracts to drive the piston to ascend or descend, the piston ascends or descends to push and pull gas in the sealing box to enter and exit the culture box, periodic pressure change is generated in the culture box, and therefore periodic pressure intervention is convenient to provide.

3. This pulsed cell hypoxia high pressure culture apparatus through the temperature in the temperature sensor monitoring incubator, then with data transmission to singlechip, the size of target setting value and monitoring value is compared to the singlechip, and when being less than the target setting value, singlechip control heater strip heating makes the temperature rise in the incubator, and when the temperature exceeded the target value in the incubator, singlechip control heater strip stop heating, the temperature reduces gradually in the messenger incubator.

4. This pulsed cell hypoxemia high pressure culture apparatus, the carbon dioxide concentration in the carbon dioxide sensor real-time supervision incubator, when the carbon dioxide concentration in the incubator was crossed lowly, single chip microcomputer control opened the solenoid valve of carbon dioxide pipe, let in carbon dioxide, when the carbon dioxide concentration in the incubator was too high, single chip microcomputer control opened the solenoid valve of nitrogen gas pipe or oxygen hose, let in nitrogen gas or oxygen to dilute carbon dioxide, make concentration reduce.

5. This pulsed cell hypoxemia high pressure culture apparatus, the oxygen concentration in the oxygen concentration sensor real-time supervision incubator, when the oxygen concentration in the incubator is low excessively, single chip microcomputer control opens the solenoid valve of oxygen pipe, lets in oxygen, and when the oxygen concentration in the incubator is too high, single chip microcomputer control opens the solenoid valve of nitrogen gas pipe or carbon dioxide pipe, lets in nitrogen gas or carbon dioxide to dilute oxygen, make concentration reduce.

6. This pulsed cell hypoxemia high pressure culture apparatus, pressure sensor real-time supervision incubator in, when pressure in the incubator was crossed low, single chip microcomputer control opened the solenoid valve, lets in gas with increased pressure, when the pressure in the incubator was too high, single chip microcomputer control opened the release valve, and exhaust gas makes pressure reduction.

7. This pulsed cell hypoxia high pressure culture apparatus, the control fan operation can make the air current in the incubator obtain the circulation to keep the uniformity of incubator internal environment parameter.

Drawings

FIG. 1 is a schematic view of the overall structure of the present invention;

fig. 2 is a cross-sectional view of the overall structure of the present invention.

In the figure: 1. an incubator; 101. an outer housing; 102. heating wires; 103. an inner housing; 2. a water pan; 3. a support plate; 4. a cell culture dish; 5. a sensor group; 6. a touch screen; 7. an oxygen tube; 8. a nitrogen gas pipe; 9. a carbon dioxide tube; 10. a filter; 11. an electromagnetic valve; 12. a fan; 13. an air escape pipe; 14. a gas release valve; 15. a communicating pipe; 16. a sealing box; 17. an electric push rod; 18. a piston.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

In the description of the present invention, it should be noted that the terms "vertical", "upper", "lower", "horizontal", and the like indicate orientations or positional relationships based on those shown in the drawings, and are only for convenience of describing the present invention and simplifying the description, but do not indicate or imply that the referred device or element must have a specific orientation, be constructed in a specific orientation, and be operated, and thus, should not be construed as limiting the present invention.

In the description of the present invention, it should also be noted that, unless otherwise explicitly specified or limited, the terms "disposed," "mounted," "connected," and "connected" are to be construed broadly and may, for example, be fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meanings of the above terms in the present invention can be understood by those skilled in the art according to specific situations.

Referring to fig. 1-2, a pulse type low-oxygen and high-pressure cell culture device comprises an incubator 1, wherein the inner wall of the incubator 1 is made of mirror stainless steel, a water tray 2 is fixedly installed on the inner bottom wall of the incubator 1, a supporting plate 3 is fixedly installed on the inner side wall of the incubator 1, and a cell culture dish 4 is arranged at the top of the supporting plate 3;

the fan 12 and the sensor group 5 are fixedly arranged on the inner top wall of the incubator 1, and the fan 12 can circulate the airflow in the incubator 1 so as to keep the consistency of the environmental parameters in the incubator 1;

an oxygen tube 7, a nitrogen tube 8 and a carbon dioxide tube 9 are fixedly installed on the outer wall of the incubator 1, one ends of the oxygen tube 7, the nitrogen tube 8 and the carbon dioxide tube 9 are communicated with the inside of the incubator 1, and the other ends of the oxygen tube 7, the nitrogen tube 8 and the carbon dioxide tube 9 are respectively and fixedly connected with an oxygen tank, a nitrogen tank and a carbon dioxide tank;

the outer wall of the incubator 1 is further connected with a communicating pipe 15 in a penetrating manner, and the tail end of the communicating pipe 15 is fixedly connected with a pulse pressure generator which is used for periodically pressing air into the incubator 1 through the communicating pipe 15 to enable the pressure in the incubator 1 to vibrate.

In this embodiment, the incubator 1 includes an outer casing 101 and an inner casing 103, and heating wires 102 are uniformly arranged in a gap between the outer casing 101 and the inner casing 103.

In the present embodiment, the sensor group 5 includes a temperature sensor, a humidity sensor, an oxygen concentration sensor, a carbon dioxide concentration sensor, and a pressure sensor.

In this embodiment, the oxygen pipe 7, the nitrogen pipe 8 and the carbon dioxide pipe 9 are all provided with a filter 10 and an electromagnetic valve 11, and the filter 10 can be a 0.2um disk filter pipe or other equivalent filter pipes, and can filter out dust or impurities in the gas.

In this embodiment, the outer wall of incubator 1 still fixed mounting has run-flat 13, and run-flat 13 is linked together with the inside of incubator 1, and the inside of run-flat 13 is provided with snuffle valve 14, and when the pressure in incubator 1 was too high, single chip microcomputer control opened snuffle valve 14, can the exhaust gas make pressure reduction.

In this embodiment, the pulse pressure generator comprises a seal box 16, an electric push rod 17 and a piston 18, the electric push rod 17 is fixedly installed on the top of the seal box 16, the piston 18 is fixedly installed on the top of an output shaft of the electric push rod 17, the piston 18 is movably arranged inside the seal box 16, and the seal box 16 is communicated with the incubator 1 through a communication pipe 15;

the single chip microcomputer controls the output shaft of the electric push rod 17 to extend or retract, the output shaft of the electric push rod 17 extends or retracts to drive the piston 18 to ascend or descend, the piston 18 ascends or descends to push and pull gas in the sealed box 16 into and out of the incubator 1, and periodic pressure change is generated in the incubator 1, so that periodic pressure intervention is provided conveniently.

In this embodiment, still include touch-control screen 6 and singlechip, touch-control screen 6 fixed mounting is on the outer wall of incubator 1 for the input of parameter and the demonstration of data.

In this embodiment, the humidity in the incubator 1 is maintained by evaporation of water in the water tray 2.

In this embodiment, the output ends of the sensor group 5 and the touch screen 6 are electrically connected to the input end of the single chip, and the output end of the single chip is electrically connected to the input ends of the heating wire 102, the electromagnetic valve 11, the air release valve 14 and the electric push rod 17 respectively.

In this embodiment, the temperature in the incubator 1 is usually 37 ℃, the carbon dioxide concentration is usually 5%, and the oxygen concentration is usually 0.1 to 21.0%, and can be adjusted as needed.

The working principle and the using process of the utility model are as follows:

the single chip microcomputer controls the output shaft of the electric push rod 17 to extend or retract, the output shaft of the electric push rod 17 extends or retracts to drive the piston 18 to ascend or descend, the piston 18 ascends or descends to push and pull the gas in the seal box 16 into and out of the incubator 1, and the periodic pressure change is generated in the incubator 1, so that the periodic pressure intervention is provided conveniently;

monitoring the temperature in the incubator 1 through a temperature sensor, transmitting data to a single chip microcomputer, comparing a target set value with a monitored value by the single chip microcomputer, controlling the heating wire 102 to heat by the single chip microcomputer when the target set value is smaller than the target set value, so that the temperature in the incubator 1 is raised, and controlling the heating wire 102 to stop heating by the single chip microcomputer when the temperature in the incubator 1 exceeds the target value, so that the temperature in the incubator 1 is gradually lowered;

the carbon dioxide sensor monitors the concentration of carbon dioxide in the incubator 1 in real time, when the concentration of the carbon dioxide in the incubator 1 is too low, the single chip microcomputer controls to open the electromagnetic valve 11 of the carbon dioxide tube 9 and introduce the carbon dioxide, and when the concentration of the carbon dioxide in the incubator 1 is too high, the single chip microcomputer controls to open the electromagnetic valve 11 of the nitrogen tube 8 or the oxygen tube 7 and introduce nitrogen or oxygen to dilute the carbon dioxide so as to reduce the concentration;

the oxygen concentration sensor monitors the oxygen concentration in the incubator 1 in real time, when the oxygen concentration in the incubator 1 is too low, the single chip microcomputer controls to open the electromagnetic valve 11 of the oxygen tube 7 and introduce oxygen, and when the oxygen concentration in the incubator 1 is too high, the single chip microcomputer controls to open the electromagnetic valve 11 of the nitrogen tube 8 or the carbon dioxide tube 9 and introduce nitrogen or carbon dioxide to dilute the oxygen so as to reduce the concentration;

the pressure sensor monitors the pressure in the incubator 1 in real time, when the pressure in the incubator 1 is too low, the single chip microcomputer controls to open the electromagnetic valve 11, gas is introduced to increase the pressure, when the pressure in the incubator 1 is too high, the single chip microcomputer controls to open the gas release valve 14, and the gas is discharged to reduce the pressure;

controlling the operation of fan 12 also allows the air flow inside incubator 1 to be circulated, thus maintaining the consistency of the environmental parameters inside incubator 1.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the utility model, the scope of which is defined in the appended claims and their equivalents.

Claims

1. The utility model provides a pulsed cell hypoxia high-pressure culture apparatus, includes incubator (1), its characterized in that: a water tray (2) is fixedly arranged on the inner bottom wall of the incubator (1), a supporting plate (3) is fixedly arranged on the inner side wall of the incubator (1), and a cell culture dish (4) is arranged at the top of the supporting plate (3);

a fan (12) and a sensor group (5) are fixedly mounted on the inner top wall of the incubator (1), an oxygen pipe (7), a nitrogen pipe (8) and a carbon dioxide pipe (9) are fixedly mounted on the outer wall of the incubator (1), one ends of the oxygen pipe (7), the nitrogen pipe (8) and the carbon dioxide pipe (9) are communicated with the inside of the incubator (1), and the other ends of the oxygen pipe (7), the nitrogen pipe (8) and the carbon dioxide pipe (9) are respectively and fixedly connected with an oxygen tank, a nitrogen tank and a carbon dioxide tank;

the outer wall of incubator (1) still through connection has communicating pipe (15), the terminal fixedly connected with pulse pressure generator of communicating pipe (15), pulse pressure generator is used for through communicating pipe (15) to the interior periodic air of impressing of incubator (1), produces the pressure oscillation in messenger incubator (1).

2. The pulsed hypoxic high-pressure culture device for cells according to claim 1, wherein: the incubator (1) comprises an outer shell (101) and an inner shell (103), and heating wires (102) are uniformly arranged in a gap between the outer shell (101) and the inner shell (103).

3. The pulsed hypoxic high-pressure culture device for cells according to claim 2, wherein: the sensor group (5) comprises a temperature sensor, a humidity sensor, an oxygen concentration sensor, a carbon dioxide concentration sensor and a pressure sensor.

4. The pulsed hypoxic high-pressure culture device for cells according to claim 3, wherein: and a filter (10) and an electromagnetic valve (11) are arranged in the oxygen pipe (7), the nitrogen pipe (8) and the carbon dioxide pipe (9).

5. The pulsed hypoxic high-pressure culture device for cells according to claim 4, wherein: the outer wall of incubator (1) still fixed mounting have disappointing pipe (13), disappointing pipe (13) is linked together with the inside of incubator (1), the inside of disappointing pipe (13) is provided with snuffle valve (14).

6. The pulsed hypoxic high-pressure culture device for cells according to claim 5, wherein: the pulse pressure generator comprises a seal box (16), an electric push rod (17) and a piston (18), wherein the electric push rod (17) is fixedly installed at the top of the seal box (16), the piston (18) is fixedly installed at the top of an output shaft of the electric push rod (17), the piston (18) is movably arranged inside the seal box (16), and the seal box (16) is communicated with the incubator (1) through a communicating pipe (15).

7. The pulsed hypoxic high-pressure culture device for cells according to claim 6, wherein: the incubator further comprises a touch screen (6) and a single chip microcomputer, wherein the touch screen (6) is fixedly installed on the outer wall of the incubator (1).

8. The pulsed hypoxic high-pressure culture device for cells according to claim 7, wherein: the output ends of the sensor group (5) and the touch screen (6) are electrically connected with the input end of the single chip microcomputer, and the output end of the single chip microcomputer is electrically connected with the input ends of the heating wire (102), the electromagnetic valve (11), the air release valve (14) and the electric push rod (17) respectively.