CN117264764A - Automatic stem cell culture equipment imitating human body internal environment - Google Patents
Automatic stem cell culture equipment imitating human body internal environment Download PDFInfo
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- CN117264764A CN117264764A CN202310486443.5A CN202310486443A CN117264764A CN 117264764 A CN117264764 A CN 117264764A CN 202310486443 A CN202310486443 A CN 202310486443A CN 117264764 A CN117264764 A CN 117264764A
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- 238000004113 cell culture Methods 0.000 title claims abstract description 62
- 210000000130 stem cell Anatomy 0.000 title claims abstract description 23
- 241000282414 Homo sapiens Species 0.000 title claims abstract description 19
- 230000001105 regulatory effect Effects 0.000 claims abstract description 34
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims abstract description 25
- 239000001301 oxygen Substances 0.000 claims abstract description 25
- 229910052760 oxygen Inorganic materials 0.000 claims abstract description 25
- 239000007789 gas Substances 0.000 claims abstract description 10
- 238000001727 in vivo Methods 0.000 claims abstract description 8
- 230000001580 bacterial effect Effects 0.000 claims description 29
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 claims description 28
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims description 21
- 239000001569 carbon dioxide Substances 0.000 claims description 14
- 229910002092 carbon dioxide Inorganic materials 0.000 claims description 14
- 239000002184 metal Substances 0.000 claims description 12
- 229910052757 nitrogen Inorganic materials 0.000 claims description 10
- 239000012535 impurity Substances 0.000 claims description 4
- 241000894006 Bacteria Species 0.000 claims description 3
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 2
- 239000000741 silica gel Substances 0.000 claims description 2
- 229910002027 silica gel Inorganic materials 0.000 claims description 2
- 210000004027 cell Anatomy 0.000 abstract description 10
- 238000000034 method Methods 0.000 abstract description 6
- 238000000338 in vitro Methods 0.000 abstract description 5
- 238000011161 development Methods 0.000 abstract description 4
- 238000002659 cell therapy Methods 0.000 abstract description 3
- 230000036772 blood pressure Effects 0.000 abstract description 2
- 239000001963 growth medium Substances 0.000 abstract description 2
- 230000008569 process Effects 0.000 abstract description 2
- 238000001125 extrusion Methods 0.000 abstract 1
- 239000000835 fiber Substances 0.000 abstract 1
- 238000013508 migration Methods 0.000 abstract 1
- 230000005012 migration Effects 0.000 abstract 1
- 239000003570 air Substances 0.000 description 64
- 238000004519 manufacturing process Methods 0.000 description 5
- 238000005516 engineering process Methods 0.000 description 4
- 230000007613 environmental effect Effects 0.000 description 3
- 230000010261 cell growth Effects 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 230000035755 proliferation Effects 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 1
- 238000010923 batch production Methods 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000004891 communication Methods 0.000 description 1
- 230000001276 controlling effect Effects 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 210000001808 exosome Anatomy 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- JCXJVPUVTGWSNB-UHFFFAOYSA-N nitrogen dioxide Inorganic materials O=[N]=O JCXJVPUVTGWSNB-UHFFFAOYSA-N 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 238000004088 simulation Methods 0.000 description 1
- 238000002660 stem cell treatment Methods 0.000 description 1
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- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/26—Means for regulation, monitoring, measurement or control, e.g. flow regulation of pH
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- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
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- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
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- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
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- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
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Abstract
An automatic stem cell culture device imitating human body internal environment belongs to the technical field of biological equipment. According to the invention, a central controller is arranged outside an incubator, a pressure regulating module and a four-mixing air inlet valve are arranged at the upper end part of the incubator, a base is arranged inside the incubator, a shaking table is fixedly arranged on the base, and a cell culture tank is detachably arranged on the shaking table; the cell culture tank is a closed container, various controllable mixed gases are introduced in the culture process to apply a periodically-changing pressure field, the dissolved oxygen concentration and the pH value of a culture medium are maintained, the culture environment of cells is more similar to the in-vivo environment with blood pressure, the control of key parameters of the cell culture environment imitating the in-vivo environment of a human body is realized by a multi-physical-field decoupling method of the environment, the characteristics of greatly improving the fiber toughness, the extrusion resistance and the migration resistance of in-vitro cultured stem cells are provided, and the technical support is provided for the development of the cell therapy industry in China.
Description
Technical Field
The invention belongs to the technical field of biological equipment, and particularly relates to automatic stem cell culture equipment imitating human body internal environment.
Background
Cell therapy is an important direction of medical science and technology development, and has remarkable curative effect in treating some serious and problematic diseases of human beings. The need for large-scale, high-quality clinical cell use has forced us to develop new cell culture devices.
In order to improve the production quality of stem cells and ensure various physiological indexes of the cells, the culture environment can be accurately controlled during the in-vitro culture of the cells. The existing cell culture equipment mainly detects and regulates the pH, temperature and dissolved oxygen concentration in the cell culture environment, for example Chen Haijia in a patent No. CN115803428A, which is a method for efficiently preparing exosomes by utilizing a stem cell scale culture device, the pH, temperature and dissolved oxygen in a reaction kettle are monitored and regulated by a culture microenvironment regulating system. However, research proves that the pressure of the environment in the human body has an influence on the cell state, proliferation speed, pluripotency and stability of stem cells, and in the existing stem cell culture process, the pressure, pH, dissolved oxygen concentration and other environmental parameters are difficult to accurately regulate and control, so that a controllable pressure culture environment is not arranged. The existing stem cell culture method still has the problem that the control of environmental factors such as pressure, dissolved oxygen, pH and the like can not be simultaneously regulated, so that the cell state, proliferation speed and the like are different from those of the actual living in vivo, and the development of stem cell treatment is hindered. It is therefore desirable to invent a stem cell culture apparatus that mimics the in vivo environment of a human including blood pressure.
In addition, compared with the traditional manual culture equipment, the automatic production equipment can remarkably improve the production efficiency, and continuous and high-flux culture operation is realized, so that complicated and time-consuming steps possibly occurring in manual operation are avoided. Meanwhile, the culture environment can be monitored and regulated in real time by matching with an advanced control system and a sensor technology, and the stability and consistency of culture conditions are ensured, so that the efficiency and quality of stem cell culture are improved.
In summary, there is a need for a low-loss, expandable, fully automatic and large-scale stem cell culture device, which can couple and regulate the pH, pressure and dissolved oxygen concentration of a culture environment in a cell culture process, so as to achieve the purpose of simulating the cell growth environment in a human body in vitro, and promote the quantity and quality of the cultured stem cells, thereby promoting the development of the cell therapy industry in China.
Disclosure of Invention
The invention aims to solve the problems of the existing cell in-vitro culture technology, and provides an automatic stem cell culture device imitating the in-vivo environment of a human body.
The application provides automatic stem cell culture equipment imitating human internal environment, a central controller is arranged outside an incubator, a pressure regulating module, an air flow regulator, a nitrogen flow regulator, a carbon dioxide flow regulator, an oxygen flow regulator and a four-mixing air inlet valve are arranged at the upper end inside the incubator, a base is arranged at the lower end inside the incubator, a shaking table is fixedly arranged on the base, and a cell culture tank is detachably arranged on the shaking table; the cell culture tank is divided into a tank cover and a tank body; the pressure sensor and the sensor group are arranged in the cell culture tank; the tank cover comprises a metal cover, a rubber ring, a locking ring and a locking bolt; wherein the rubber ring is fixed in the groove on the metal cover, and the rubber ring is connected with the cell culture tank; the locking ring is wrapped and tightly fixed at the upper end of the outer wall of the cell culture tank, the metal cover is provided with a plurality of threaded holes, the threaded holes correspond to the plurality of through holes on the locking ring one by one, and the plurality of locking bolts are screwed with the threaded holes on the metal cover through the through holes on the locking ring, so that the tank cover and the cell culture tank form a closed system; an air inlet pipe, a first air pipe and a second air pipe are fixedly arranged on the tank cover; the sensor group is detachably assembled at the lower end of the interior of the cell culture tank; the air flow regulator, the nitrogen flow regulator, the carbon dioxide flow regulator and the oxygen flow regulator are connected with corresponding air cylinders, and the other end of the air flow regulator is connected with the four-mixing air inlet valve through an air pipe; the pressure regulating proportional valve in the pressure regulating module is fixedly arranged on the cover of the tank cover, an air inlet port of the pressure regulating proportional valve is connected with the safety valve through an air pipe, the safety valve is connected with one end of the second bacterial filter through the air pipe, and the other port of the second bacterial filter is detachably arranged on the first air pipe; the air return port of the pressure regulating proportional valve is connected with one end of a third bacterial filter through an air pipe, and the other port of the third bacterial filter is detachably arranged on a second air pipe; the exhaust port of the pressure regulating proportional valve is directly communicated with the outside air;
the sensor group comprises a temperature sensor, a dissolved oxygen concentration sensor, a pH sensor and a carbon dioxide concentration sensor.
The first bacterial filter, the second bacterial filter and the third bacterial filter can bidirectionally filter fine impurities and bacteria in the gas.
The cell culture tank is a closed pressure-resistant container.
The invention has the following advantages:
(1) The invention realizes the control of the key environment parameters of the human body environment simulation culture based on the decoupling of multiple physical fields. The existing cell culture equipment and method can not solve the problem of regulating and controlling the pressure, pH, dissolved oxygen concentration and temperature in the culture environment at the same time; based on a feedback signal of a high-sensitivity sensor, decoupling analysis is performed on a plurality of physical fields of a cell culture environment through a built-in control model, the cell culture temperature is regulated through a heater and a temperature controller in a comprehensive regulation manner, and after the temperature is stable, the pressure, the pH value and the dissolved oxygen concentration in the cell growth environment are controlled through regulating the proportion, the speed, the time and the like of four gases of air, oxygen, nitrogen and carbon dioxide which are introduced into a cell culture tank; solves the problem that the prior art can not regulate and control the environmental pressure, pH, dissolved oxygen concentration and temperature simultaneously when culturing in vitro, so that the cells are always in the culture environment similar to human body, and the production quality of the cells is ensured to the greatest extent.
(2) The invention realizes the automatic cell culture based on the invention point (1). At present, a labor-intensive cell culture mode is adopted, such as manual addition of culture medium, sampling, detection, expansion of a culture system and the like, most of operation steps are easy to cause pollution and generate larger errors, so that automatic operation of equipment is changed, batch production of cells can be completed without a large number of experimenters, the repeatability and stability of a cell culture process are improved, and production requirements are met.
Drawings
FIG. 1 is a schematic diagram of a connection structure of an automated stem cell culture apparatus that mimics the in vivo environment of a human being;
FIG. 2 is a schematic diagram of a pressure regulation module connection configuration;
FIG. 3 is an assembly drawing of a cell culture tank cover and a tank body in an automated stem cell culture apparatus simulating a human internal environment.
The reference numerals in the drawings:
1. a central controller; 2. a pressure regulating module; 3. a can lid; 4. a pressure sensor; 5. a cell culture tank; 6. a sensor group; 7. shaking table; 8. a base; 9. an air inlet pipe; 9.1, a first bacterial filter; 10. an air flow regulator; 11. a nitrogen flow regulator; 12. a carbon dioxide flow regulator; 13. an oxygen flow regulator; 14. a four-mixing air inlet valve; 15. a warm box; 2.1, a first air pipe; 2.2, a second bacterial filter; 2.3, a safety valve; 2.5, a second air pipe; 2.6, a third bacterial filter.
Detailed Description
Various exemplary embodiments, features and aspects of the present application will be described in detail below with reference to the accompanying drawings. In the drawings, like reference numbers indicate identical or functionally similar elements. Although various aspects of the embodiments are illustrated in the accompanying drawings, the drawings are not necessarily drawn to scale unless specifically indicated. The word "exemplary" is used herein to mean "serving as an example, embodiment, or illustration. Any embodiment described herein as "exemplary" is not necessarily to be construed as preferred or advantageous over other embodiments. In addition, for the purposes of better illustrating the subject application, it will be apparent to one skilled in the art that numerous specific details are set forth in the various embodiments that follow. The present application may be practiced without some of these specific details. In some embodiments, methods, means and elements well known to those skilled in the art have not been described in detail in order to facilitate the salient features of the present application.
As shown in fig. 1 to 3, the embodiment of the application provides an automatic stem cell culture device simulating an internal environment of a human body, a central controller 1 is arranged outside an incubator 15, a pressure regulating module 2, an air flow regulator 10, a nitrogen flow regulator 11, a carbon dioxide flow regulator 12, an oxygen flow regulator 13 and a four-mixing air inlet valve 14 are arranged at the upper end inside the incubator 15, a base 8 is arranged at the lower end inside the incubator 15, a shaking table 7 is fixedly arranged on the base 8, and a cell culture tank 5 is detachably arranged on the shaking table 7; the cell culture tank is divided into a tank cover 3 and a tank body; the cell culture tank 5 is internally provided with a pressure sensor 4 and a sensor group 6;
in some exemplary implementations of the present example, the can lid 3 comprises a metal lid 3.1, a rubber ring 3.2, a locking ring 3.3, a locking bolt 3.4; wherein the rubber ring 3.2 is fixed in a groove on the metal cover 3.1, and the rubber ring 3.2 is connected with the cell culture tank 5; the locking ring 3.3 is wrapped and tightly fixed at the upper end of the outer wall of the cell culture tank 5, the metal cover 3.1 is provided with a plurality of threaded holes, the threaded holes correspond to the plurality of through holes on the locking ring 3.3 one by one, and the plurality of locking bolts 3.4 are screwed with the threaded holes on the metal cover through the through holes on the locking ring 3.3, so that the tank cover 3 and the cell culture tank 5 form a closed system; an air inlet pipe 9, a first air pipe 2.1 and a second air pipe 2.5 are fixedly arranged on the tank cover 3; the four-mixing air inlet valve 14 is connected with one end of the first bacterial filter 9.1 through a silica gel pipe, the other end of the first bacterial filter 9.1 is detachably arranged on the air inlet pipe 9, one end of the air flow regulator 10, one end of the nitrogen flow regulator 11, one end of the carbon dioxide flow regulator 12 and one end of the oxygen flow regulator 13 are connected with corresponding air cylinders, and the other end of the air flow regulator is connected with the four-mixing air inlet valve 14 through an air pipe; the four gases are mixed in advance through a four-mixing-one air inlet valve 14, filtered by the first bacterial filter 9.1 to remove impurities and then introduced into the cell culture tank 5; the pressure regulating proportional valve 2.4 in the pressure regulating module 2 is fixedly arranged on the cover 3 of the tank cover, an air inlet port of the pressure regulating proportional valve 2.4 is connected with the safety valve 2.3 through an air pipe, the safety valve 2.3 is connected with one end of the second bacterial filter 2.2 through the air pipe, and the other port of the second bacterial filter 2.2 is detachably arranged on the first air pipe 2.1; the air return port of the pressure regulating proportional valve 2.4 is connected with one end of the third bacterial filter 2.6 through an air pipe, and the other port of the third bacterial filter 2.6 is detachably arranged on the second air pipe 2.5; the exhaust port of the pressure regulating proportional valve 2.4 is directly communicated with the outside air; the air inlet port of the pressure regulating proportional valve 2.4 in the pressure regulating module 2 is normally opened to maintain the normal working state of the module, and the air return port and the air exhaust port form a certain opening proportion to maintain the pressure stability in the cell culture tank; the safety valve 2.3 plays a role in safety protection in the equipment, and when the air pressure in the cell culture tank exceeds a specified value, the safety valve 2.3 is opened, so that the cell culture tank 5 is ensured not to have accidents due to overhigh pressure.
The central controller 1 is connected with the pressure sensor 4 and the sensor group 6 through a data line to acquire measurement signals; the central controller 1 is connected with the pressure regulating module 2, the shaking table 7, the incubator 15, the air flow regulator 10, the nitrogen flow regulator 11, the carbon dioxide flow regulator 12, the oxygen flow regulator 13 and the four-mixing air inlet valve 14 through communication lines for feedback control.
The pH, dissolved oxygen concentration and pressure control of the culture environment are realized by means of the regulation and control of the central controller 1 on the pressure regulating module 2 and a mixed air inlet device, wherein the mixed air inlet device comprises an air flow regulator 10, a nitrogen flow regulator 11, a carbon dioxide flow regulator 12, an oxygen flow regulator 13 and a four-mixing air inlet valve 14; firstly, taking a target set value into an internal model, calculating mass proportion coefficients of carbon dioxide, oxygen, nitrogen and air which need to be introduced into a cell culture tank 5, starting a mixed air inlet device by a central controller 1 to continuously introduce mixed air, and starting a pressure regulating module 2 by the central controller 1 at the same time, and starting stable regulation of the pressure in the cell culture tank 5; when the sensor group detects that the pH value and/or the numerical value in the dissolved oxygen concentration change, the central controller 1 calculates the required introducing proportion of the four gases according to the model, controls the flow regulators of the four gases in the mixed air inlet device, and continuously introduces the mixed gases into the cell culture tank 5 until the numerical value detected by the sensor group 6 returns to the set range after the introducing proportion of the four mixed gases is changed; in the process of continuously feeding air into the mixed air feeding device, the pressure regulating module 2 needs to regulate the opening degrees of the air inlet port, the air return port and the air exhaust port of the pressure regulating proportional valve 2.4 at any time according to the detection value fed back by the pressure sensor 4 so as to keep the pressure in the cell culture tank 5 stable.
The sensor group 6 comprises a temperature sensor, a dissolved oxygen concentration sensor, a pH sensor and a carbon dioxide concentration sensor.
The first bacterial filter 9.1, the second bacterial filter 2.2 and the third bacterial filter 2.6 can bidirectionally filter fine impurities and bacteria in the gas.
The cell culture tank 5 is a closed pressure-resistant container.
The embodiments of the present application have been described above, the foregoing description is exemplary, not exhaustive, and not limited to the embodiments disclosed. Many modifications and variations will be apparent to those of ordinary skill in the art without departing from the scope and spirit of the various embodiments described. The terminology used herein was chosen in order to best explain the principles of the embodiments, the practical application, or the improvement of technology in the marketplace, or to enable others of ordinary skill in the art to understand the embodiments disclosed herein.
Claims (4)
1. An automatic stem cell culture device imitating human body internal environment is characterized in that a central controller (1) is arranged outside an incubator (15), a pressure regulating module (2), an air flow regulator (10), a nitrogen flow regulator (11), a carbon dioxide flow regulator (12), an oxygen flow regulator (13) and a four-mixing air inlet valve (14) are arranged at the upper end inside the incubator (15), a base (8) is arranged at the lower end inside the incubator (15), a shaking table (7) is fixedly arranged on the base (8), and a cell culture tank (5) is detachably arranged on the shaking table (7); the cell culture tank (5) is divided into a tank cover (3) and a tank body; the cell culture tank (5) is internally provided with a pressure sensor (4) and a sensor group (6);
the can cover (3) is characterized by comprising a metal cover (3.1), a rubber ring (3.2), a locking ring (3.3) and a locking bolt (3.4); wherein the rubber ring (3.2) is fixed in a groove on the metal cover (3.1), and the rubber ring (3.2) is connected with the cell culture tank (5); the locking ring (3.3) is wrapped and tightly fixed at the upper end of the outer wall of the tank body, a plurality of threaded holes are formed in the metal cover (3.1), the locking ring (3.3) is in one-to-one correspondence with a plurality of through holes, and a plurality of locking bolts (3.4) are screwed with the threaded holes in the metal cover through the through holes in the locking ring (3.3), so that the tank cover (3) and the tank body form a closed system; an air inlet pipe (9), a first air pipe (2.1) and a second air pipe (2.5) are fixedly arranged on the tank cover (3); the air inlet valve (14) is connected with one end of the first bacterial filter (9.1) through a silica gel pipe, the other end of the first bacterial filter (9.1) is detachably arranged on the air inlet pipe (9), one end of the air flow regulator (10), one end of the nitrogen flow regulator (11), one end of the carbon dioxide flow regulator (12) and one end of the oxygen flow regulator (13) are connected with corresponding air cylinders, and the other end of the air flow regulator is connected with the air inlet valve (14) through an air pipe; the pressure regulating proportional valve (2.4) in the pressure regulating module (2) is fixedly arranged on the cover of the tank cover (3), an air inlet port of the pressure regulating proportional valve (2.4) is connected with the safety valve (2.3) through an air pipe, the safety valve (2.3) is connected with one end of the second bacterial filter (2.2) through an air pipe, and the other port of the second bacterial filter (2.2) is detachably arranged on the first air pipe (2.1); the air return port of the pressure regulating proportional valve (2.4) is connected with one end of the third bacterial filter (2.6) through an air pipe, and the other port of the third bacterial filter (2.6) is detachably arranged on the second air pipe (2.5); the exhaust port of the pressure regulating proportional valve (2.4) is directly communicated with the outside air.
2. An automated stem cell culture apparatus mimicking a human in vivo environment according to claim 1, wherein said sensor set (6) comprises a temperature sensor, a dissolved oxygen concentration sensor, a pH sensor and a carbon dioxide concentration sensor.
3. An automated stem cell culture apparatus according to claim 1, wherein the first bacterial filter (9.1), the second bacterial filter (2.2) and the third bacterial filter (2.6) are adapted to bi-directionally filter fine impurities and bacteria from the gas.
4. An automated stem cell culture apparatus simulating a human in vivo environment according to claim 1, wherein said cell culture tank (5) is a closed pressure vessel.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310486443.5A CN117264764A (en) | 2023-04-28 | 2023-04-28 | Automatic stem cell culture equipment imitating human body internal environment |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310486443.5A CN117264764A (en) | 2023-04-28 | 2023-04-28 | Automatic stem cell culture equipment imitating human body internal environment |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN117264764A true CN117264764A (en) | 2023-12-22 |
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| CN202310486443.5A Pending CN117264764A (en) | 2023-04-28 | 2023-04-28 | Automatic stem cell culture equipment imitating human body internal environment |
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20030066638A1 (en) * | 2001-08-13 | 2003-04-10 | Yuzhi Qu | Devices using a medium having a high heat transfer rate |
| CN206289259U (en) * | 2016-11-21 | 2017-06-30 | 南京鼓楼医院 | A kind of controlled oxygen temperature control air pressure pressure cell culture apparatus |
| CN110985721A (en) * | 2019-12-06 | 2020-04-10 | 眉山麦克在线设备股份有限公司 | Multi-channel electronic pressure controller |
| CN215924962U (en) * | 2021-02-10 | 2022-03-01 | 上海塔望智能科技有限公司 | Pulse type cell hypoxia high-pressure culture device |
| CN216964638U (en) * | 2021-11-30 | 2022-07-15 | 康码（上海）生物科技有限公司 | Horizontal self-stirring in-vitro protein synthesis reactor |
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Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20030066638A1 (en) * | 2001-08-13 | 2003-04-10 | Yuzhi Qu | Devices using a medium having a high heat transfer rate |
| CN206289259U (en) * | 2016-11-21 | 2017-06-30 | 南京鼓楼医院 | A kind of controlled oxygen temperature control air pressure pressure cell culture apparatus |
| CN110985721A (en) * | 2019-12-06 | 2020-04-10 | 眉山麦克在线设备股份有限公司 | Multi-channel electronic pressure controller |
| CN215924962U (en) * | 2021-02-10 | 2022-03-01 | 上海塔望智能科技有限公司 | Pulse type cell hypoxia high-pressure culture device |
| CN216964638U (en) * | 2021-11-30 | 2022-07-15 | 康码（上海）生物科技有限公司 | Horizontal self-stirring in-vitro protein synthesis reactor |
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