CN215906204U

CN215906204U - Isothermal amplification detection device with image acquisition function

Info

Publication number: CN215906204U
Application number: CN202120764802.5U
Authority: CN
Inventors: 刘国宪; 曹伟; 刘琼瑶
Original assignee: Anpu Future Changzhou Biotechnology Co ltd
Current assignee: Anpu Future Changzhou Biotechnology Co ltd
Priority date: 2021-04-15
Filing date: 2021-04-15
Publication date: 2022-02-25
Anticipated expiration: 2031-04-15

Abstract

The utility model is suitable for the technical field of biomedical detection and experimental equipment, and provides an isothermal amplification detection device with image acquisition, which comprises: a main frame body; the mixing mechanism is arranged at the top of the main frame body and comprises a PCR tube assembly, a magnetic part and a mixing driving assembly, the PCR tube assembly comprises a heating part and a PCR tube, the heating part is coated on the outer side of the PCR tube, the magnetic part is arranged on the side surface of the heating part, the mixing driving assembly is provided with a motor, the motor is arranged on the main frame body, and the motor drives the PCR tube assembly to move; the detection and acquisition mechanism comprises an excitation part and an image acquisition part, wherein the excitation part is arranged at the top of the PCR tube assembly, and the image acquisition part is arranged at the bottom of the heating part. Therefore, the utility model carries out detection by means of image acquisition and simultaneous multiple detections, simplifies the operation process and avoids personnel errors to a greater extent.

Description

Isothermal amplification detection device with image acquisition function

Technical Field

The utility model relates to the technical field of biomedical detection and experimental equipment, in particular to an isothermal amplification detection device with image acquisition.

Background

RPA (recombinase polymerase amplification technology) is a novel nucleic acid amplification technology, and is a technology for realizing exponential amplification of nucleic acid under the constant temperature condition in the presence of various enzymes and proteins. The RPA fluorescence detection is based on a fluorescent molecular probe method, and a primer marked with a fluorescent probe modified by FAM and ROX is added into a reagent to be amplified, and fluorescence is generated by fluorescence excitation after series of reactions. The method has the characteristics of high detection speed, high sensitivity, good specificity and the like. And the reaction can be amplified at the same temperature under the condition of body temperature, so that the method is particularly suitable for field diagnosis and bedside diagnosis outside a laboratory and has wide application prospect.

In the prior art, the isothermal amplification detection technology is observed manually during detection, detection and observation are required to be carried out independently, detection images cannot be acquired automatically, images are acquired independently through manpower, and workload and acquisition and detection time are greatly increased.

In view of the above, the prior art is obviously inconvenient and disadvantageous in practical use, and needs to be improved.

SUMMERY OF THE UTILITY MODEL

In view of the above-mentioned drawbacks, the present invention provides an integrated and portable fluorescence detection and analysis device integrating automatic mixing, constant temperature control, fluorescence excitation and detection. The utility model can be applied to nucleic acid detection, based on the RPA isothermal amplification principle, utilizes fluorescence to qualitatively detect the positive and negative of a sample, and detects by means of image acquisition and simultaneous multiple detections, thereby simplifying the operation process and avoiding personnel errors to a greater extent.

In order to achieve the above object, the present invention provides an isothermal amplification detection apparatus with image acquisition, comprising: a main frame body; the mixing mechanism is arranged at the top of the main frame body and comprises a PCR tube assembly, a magnetic part and a mixing driving assembly, the PCR tube assembly comprises a heating part and a PCR tube, the heating part is coated on the outer side of the PCR tube, the magnetic part is arranged on the side surface of the heating part, the mixing driving assembly is provided with a motor, the motor is arranged on the main frame body, and the motor drives the PCR tube assembly to move; the detection and acquisition mechanism comprises an excitation part and an image acquisition part, wherein the excitation part is arranged at the top of the PCR tube assembly, and the image acquisition part is arranged at the bottom of the heating part.

According to the isothermal amplification detection device with the image acquisition function, the PCR tube assembly is provided with the plurality of PCR tubes, and the plurality of PCR tubes are uniformly arranged in the heating element.

According to the isothermal amplification detection device with the image acquisition function, the heating element comprises an aluminum block and a printed heating circuit board attached to the side face of the aluminum block, and horn-shaped lighting holes which correspond to the bottoms of the PCR tubes are formed in the bottom of the aluminum block.

According to the isothermal amplification detection device with the image acquisition function, the heat-conducting silicone grease is arranged between the printed heating circuit board and the aluminum block, and the printed heating circuit board is connected with the aluminum block through screws.

According to the isothermal amplification detection device with the image acquisition function, the bottom of the image acquisition part is provided with the guide rail, the direction of the guide rail is the same as that of the heating part, and the image acquisition part can slide along the guide rail.

According to the isothermal amplification detection device with the image acquisition function, the tray is arranged at the bottom of the main frame body, and the guide rail is fixedly connected to the top of the tray.

According to the isothermal amplification detection device with image acquisition, the detection acquisition mechanism further comprises a control component, and the control component is connected with the heating element, the image acquisition element, the hybrid driving component and the image acquisition element through leads.

According to the isothermal amplification detection device with the image acquisition function, the heating element is movably connected to the main frame body, the tail part of the heating element is provided with the driven wheel, the motor is provided with the driving shaft, the top of the driving shaft is provided with the driving wheel, and the driven wheel is connected with the driving wheel through the belt.

According to the isothermal amplification detection device with image acquisition, the magnetic member is a bar magnet.

The utility model provides an isothermal amplification detection device with image acquisition, which comprises: the main frame body is used for bearing various mechanisms and assisting in supporting the device; the mixing mechanism is arranged at the top of the main frame body and used for fully mixing detection materials and increasing the detection accuracy, the mixing mechanism comprises a PCR tube assembly, a magnetic part and a mixing driving assembly, the PCR tube assembly comprises a heating part and a PCR tube, the heating part is coated on the outer side of the PCR tube and is beneficial to uniformly and fully heating the PCR tube when the heating part heats the PCR tube and preventing the phenomenon of local overheating, the magnetic part is arranged on the side surface of the heating part and used for further increasing the mixing efficiency of the PCR tube and preventing the phenomenon of uneven mixing, the mixing driving assembly is provided with a motor, the motor is arranged on the main frame body and drives the PCR tube assembly to move, the motor is increased to provide power during mixing and increase the mixing effect of the device; the detection and acquisition mechanism comprises an exciting piece and an image acquisition piece, wherein the exciting piece is arranged at the top of the PCR tube assembly, the image acquisition piece is arranged at the bottom of the heating piece, and the exciting piece is arranged at the top, so that the full activation of the fluorescein of the detection device is facilitated, and the acquisition and display of images by the subsequent image acquisition piece are facilitated.

Drawings

FIG. 1 is a schematic perspective view of the present invention;

FIG. 2 is a schematic view of the rear side of the structure of the present invention;

FIG. 3 is a schematic structural view of an 8-hole aluminum block of the present invention;

in the figure, 1-PCR tube, 2-bar magnet, 3-fixed support, 4-L type moving support, 5-lead screw, 6-driven wheel, 7-driven wheel fixed support, 8-bottom plate, 9-right side touch switch, 10-right side touch switch fixed support, 11-motor, 12-image acquisition part, 13-limit hole, 14-tray, 15-guide rail, 16-limit switch, 17-limit switch fixed support, 18-left side touch switch fixed support, 19-left side touch switch, 20-step moving motor fixed support, 21-driving wheel, 22-step moving motor, 23-belt, 24-heating part, 25-excitation part, 26-printed heating circuit board, 27-toothed plate, 28-temperature probe fixing hole.

Detailed Description

For the purpose of making the objects, technical solutions and advantages of the present invention more apparent, the present invention will be further described in detail with reference to the accompanying drawings and embodiments, it being understood that the specific embodiments described herein are merely illustrative of the present invention and are not intended to limit the present invention.

Referring to fig. 1, the utility model provides an isothermal amplification detection device with image acquisition, which comprises a mixing mechanism, wherein the mixing mechanism consists of a plurality of PCR tubes 1, a bar magnet 2, a fixed support 3, an L-shaped motion support 4, a screw rod 5, a motor 11 and a heating element 24. The PCR tube 1 contains magnetic beads, and the strip-shaped magnet 2 is driven to move up and down through the threaded fit between the motor 11, the screw rod 5 and the L-shaped moving support 4. The bar magnet 2 and the magnetic beads in the PCR tube 1 move up and down under the action of magnetic force, thereby completing the operation of mixing the reagents. After mixing, through microprocessor control motor 11, improve the magnetic bead to reagent middle part position, reduce its influence to the testing result.

Referring to fig. 2 and 3, preferably, the test acquisition mechanism of the present invention includes a heating element 24 comprising an aluminum block and a printed heating circuit board 26. The heating surface of the printed heating circuit board 26 is coated with a layer of heat-conducting silicone grease and is attached to the back of the aluminum block, and the printed heating circuit board 26 is fixed on the aluminum block through screws. The power of the printed heating circuit board 26 is determined according to the area of the heating area and the required temperature control range. Compared with common commercial heating resistance films, the heating resistance film has the characteristics of low cost, batch printing and the like. By arranging the aluminum block, the PCR tube 1 can be just placed in the aluminum block, the printed heating circuit board 26 can be additionally arranged on the back of the aluminum block, and meanwhile, a temperature measuring probe fixing hole 28 is reserved in the middle of the bottom of the aluminum block. The temperature measuring probe is fixed in the temperature measuring probe fixing hole 28 through the heat-conducting silicone grease. Real-time temperature detection and constant temperature control are completed through the microprocessor and the detection and control circuit thereof, and the aluminum block uniformly spreads heat into the PCR tube 1.

In addition, the detection and acquisition mechanism further comprises an image acquisition part 12, a tray 14, a limiting hole 13, a guide rail 15, a limiting switch 16, a limiting switch fixing support 17, a left touch switch 19, a right touch switch 9, a left touch switch fixing support 18, a right touch switch fixing support 10, a bottom plate 8, a driven wheel 6, a driven wheel fixing support 7, a movement stepping motor 22, a driving wheel 21, a stepping movement motor fixing support 20, a belt 23 and a toothed plate 27. The movement stepping motor 22 is controlled by a microprocessor to drive the driving wheel 21 to rotate, so as to drive the belt 23 to rotate, so that the toothed plate 27 fixed at the bottom of the belt 23 moves horizontally, and the toothed plate 27 is in toothed connection with the tray 14, so that the tray 14 can move on the guide rail 15 in the horizontal direction. The image capturing member 12 is secured to a tray 14. The tray 14 determines the initial position of the tray 14 through the left touch switch 19 and the right touch switch 9, and then the microprocessor controls the step number of the stepping motor 22 to ensure that the image acquisition part 12 is right below each detection position. The limit switch 16 is matched with the limit hole 13 at the side of the tray 14, so that the device cannot be damaged due to transportation or vibration.

Further, the exciting member 25 of the present invention emits FAM and ROX excitation lights, so that the reagent in the PCR tube 1 is excited and a fluorescence signal is transmitted to the image capturing member 12. The image acquisition part 12 is respectively provided with two wavelength filters of FAM and ROX to detect the fluorescence intensity of the fluorescence excitation liquid in the PCR tube 1, thereby judging the detection result.

In this example, rotate through motor 11 and drive lead screw 5 and rotate, thereby realize bar magnet 2 at heating member 24 up-and-down motion, drive the magnetic bead motion of PCR pipe 1 inside, the realization carries out abundant mixture with the detect reagent of a plurality of PCR pipe 1 insides, after mixing, through the exciting piece 25 at top, excite the activity of fluorescent agent, the fluorescent agent luminous phenomenon appears, the step motion motor 22 motion through the microprocessing unit that is control mechanism, realize the motion of tray 14, drive image acquisition 12 and be CCD camera motion, the realization carries out image acquisition to the situation of the reagent of PCR pipe 1 insides.

In summary, the present invention provides an isothermal amplification detection apparatus with image acquisition, comprising: the main frame body is used for bearing various mechanisms and assisting in supporting the device; the mixing mechanism is arranged at the top of the main frame body and used for fully mixing detection materials and increasing the detection accuracy, the mixing mechanism comprises a PCR tube assembly, a magnetic part and a mixing driving assembly, the PCR tube assembly comprises a heating part and a PCR tube, the heating part is coated on the outer side of the PCR tube and is beneficial to uniformly and fully heating the PCR tube when the heating part heats the PCR tube and preventing the phenomenon of local overheating, the magnetic part is arranged on the side surface of the heating part and used for further increasing the mixing efficiency of the PCR tube and preventing the phenomenon of uneven mixing, the mixing driving assembly is provided with a motor, the motor is arranged on the main frame body and drives the PCR tube assembly to move, the motor is increased to provide power during mixing and increase the mixing effect of the device; the detection and acquisition mechanism comprises an exciting piece and an image acquisition piece, wherein the exciting piece is arranged at the top of the PCR tube assembly, the image acquisition piece is arranged at the bottom of the heating piece, and the exciting piece is arranged at the top, so that the full activation of the fluorescein of the detection device is facilitated, and the acquisition and display of images by the subsequent image acquisition piece are facilitated.

The present invention may be embodied in other specific forms without departing from the spirit or essential attributes thereof, and it should be understood that various changes and modifications can be effected therein by one skilled in the art without departing from the spirit and scope of the utility model as defined in the appended claims.

In the description of the present invention, it is to be understood that the terms "center", "longitudinal", "lateral", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", and the like, indicate orientations or positional relationships based on those shown in the drawings, and are used only for convenience in describing the present invention and for simplicity in description, and do not indicate or imply that the referenced devices or elements must have a particular orientation, be constructed and operated in a particular orientation, and thus, are not to be construed as limiting the present invention. Furthermore, the terms "first", "second", etc. are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first," "second," etc. may explicitly or implicitly include one or more of that feature. In the description of the present invention, "a plurality" means two or more unless otherwise specified.

Claims

1. An isothermal amplification detection device with image acquisition, comprising:

a main frame body;

the mixing mechanism is arranged at the top of the main frame body and comprises a PCR tube assembly, a magnetic part and a mixing driving assembly, the PCR tube assembly comprises a heating part and a PCR tube, the heating part is coated on the outer side of the PCR tube, the magnetic part is arranged on the side surface of the heating part, the mixing driving assembly is provided with a motor, the motor is arranged on the main frame body, and the motor drives the PCR tube assembly to move;

the detection and acquisition mechanism comprises an excitation part and an image acquisition part, wherein the excitation part is arranged at the top of the PCR tube assembly, and the image acquisition part is arranged at the bottom of the PCR tube assembly.

2. The isothermal amplification detection device with image acquisition of claim 1, wherein the PCR tube assembly is provided with a plurality of PCR tubes, and the plurality of PCR tubes are uniformly disposed inside the heating element.

3. The isothermal amplification detection device with image acquisition of claim 2, wherein the heating element comprises an aluminum block and a printed heating circuit board attached to the side surface of the aluminum block, and the bottom of the aluminum block is provided with horn-shaped lighting holes corresponding to the bottoms of the PCR tubes.

4. The isothermal amplification detection device with image acquisition of claim 3, wherein a heat conducting silicone grease is arranged between the printed heating circuit board and the aluminum block, and the printed heating circuit board is connected with the aluminum block by a screw.

5. The isothermal amplification detection device with image acquisition of claim 1, wherein a guide rail is arranged at the bottom of the image acquisition member, the guide rail is in the same direction as the heating element, and the image acquisition member can slide along the guide rail.

6. The isothermal amplification detection device with image acquisition of claim 5, wherein a tray is arranged at the bottom of the main frame body, and the guide rail is fixedly connected to the top of the tray.

7. The isothermal amplification detection device with image capture of claim 1, wherein the detection and capture mechanism further comprises a control module in wired connection with the heating element, the image capture element, the hybrid drive module and the image capture element.

8. The isothermal amplification detection device with image acquisition of claim 1, wherein the heating element is movably connected to the main frame, the driven wheel is disposed at the tail of the heating element, the motor has a driving shaft, the driving shaft has a driving wheel at the top, and the driven wheel is connected to the driving wheel via a belt.

9. The isothermal amplification detection device with image acquisition of claim 1, wherein the magnetic member is a bar magnet.