CN215894506U - Pump-controlled intelligent multidimensional liquid chromatograph - Google Patents
Pump-controlled intelligent multidimensional liquid chromatograph Download PDFInfo
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- CN215894506U CN215894506U CN202122392682.3U CN202122392682U CN215894506U CN 215894506 U CN215894506 U CN 215894506U CN 202122392682 U CN202122392682 U CN 202122392682U CN 215894506 U CN215894506 U CN 215894506U
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Abstract
The utility model discloses a pump-controlled intelligent multi-dimensional liquid chromatograph, which comprises a first chromatographic pump, a second chromatographic pump, a third chromatographic pump, a fourth chromatographic pump, a first chromatographic column, a second chromatographic column, a third chromatographic column and a fourth chromatographic column, wherein the first chromatographic pump is connected with a sample injector, the sample injector is connected with a first multi-way valve, the second chromatographic pump is connected with a second multi-way valve, the fourth chromatographic pump is connected with the second multi-way valve, the first chromatographic column is connected with any two ports of the first multi-way valve, the liquid inlet end of the second chromatographic column is connected with the first multi-way valve, and the liquid outlet end of the second chromatographic column is respectively connected with the third chromatographic pump and the second multi-way valve through a tee joint; the third chromatographic column is connected with any two ports of the second multi-way valve, the liquid inlet end of the fourth chromatographic column is connected with the second multi-way valve, and the liquid outlet end of the fourth chromatographic column is connected with the detector; the first waste liquid pool is connected with the first multi-way valve, and the second waste liquid pool is connected with the second multi-way valve. The utility model does not need solvent switching before pumping, thereby avoiding the risks of cross contamination of pipeline liquid and the like.
Description
Technical Field
The utility model relates to the field of liquid chromatography, in particular to a pump-controlled intelligent multi-dimensional liquid chromatograph.
Background
Since macromolecular impurities such as protein contained in the biological sample easily interfere the detection of small molecular compounds and easily cause pipeline blockage and the degradation of the first-dimension online analytical column in different degrees, the problems can be solved by adopting the online extraction and liquid chromatograph technology. The liquid chromatography technology combined with online extraction firstly removes macromolecular impurities such as protein and the like through online solid-phase extraction, traps micromolecular compounds and improves peak capacity, thereby effectively improving the separation and sensitivity of micromolecular compound components in complex samples. However, the existing off-line solid phase extraction technology needs manual pretreatment, and has the problems of time consumption, large error and the like, and the conventional on-line solid phase extraction technology and the two-dimensional liquid chromatography technology have poor impurity removal effect and cannot directly feed large-volume samples such as plasma, serum and the like.
Disclosure of Invention
The utility model aims to provide a pump-controlled intelligent multi-dimensional liquid chromatograph capable of carrying out whole plasma and serum sample injection aiming at the defects of the prior art.
The technical scheme of the utility model is as follows:
a pump-controlled intelligent multi-dimensional liquid chromatograph comprises a sample injector, a first waste liquid pool, a second waste liquid pool, a detector, a first multi-way valve and a second multi-way valve, a first chromatographic pump, a second chromatographic pump, a third chromatographic pump, a fourth chromatographic pump, a first chromatographic column, a second chromatographic column, a third chromatographic column and a fourth chromatographic column, wherein the first multi-way valve and the second multi-way valve are used for connecting and switching pipelines, the first chromatographic pump is connected with the input end of the sample injector, the output end of the sample injector is connected with the first multi-way valve, the second chromatographic pump is connected with the first multi-way valve, the fourth chromatographic pump is connected with the second multi-way valve, the first chromatographic column is connected with any two ports of the first multi-way valve, the liquid inlet end of the second chromatographic column is connected with the first multi-way valve, and the liquid outlet end of the second chromatographic column is respectively connected with the third chromatographic pump and the second multi-way valve through a tee joint; the third chromatographic column is connected with any two ports of the second multi-way valve, the liquid inlet end of the fourth chromatographic column is connected with the second multi-way valve, and the liquid outlet end of the fourth chromatographic column is connected with the detector; the first waste liquid pool is connected with the first multi-way valve, and the second waste liquid pool is connected with the second multi-way valve.
Further, the first chromatographic column is an online solid-phase extraction column for bidirectional separation; the second chromatographic column and the third chromatographic column are online analytical columns or online solid-phase extraction columns; the fourth chromatographic column is an online analytical column.
The first multi-way valve and the second multi-way valve are both two-position six-way valves, and each two-position six-way valve comprises a first port, a second port, a third port, a fourth port, a fifth port and a sixth port which are sequentially arranged along the anticlockwise direction;
the first port and the fourth port of the first multi-way valve are connected with two ends of the first chromatographic column, the second port of the first multi-way valve is connected with the output end of the sample injector, the input end of the sample injector is connected with the first chromatographic pump, the third port of the first multi-way valve is connected with the first waste liquid pool, the fifth port of the first multi-way valve is connected with the second chromatographic pump, the sixth port of the first multi-way valve is connected with the liquid inlet end of the second chromatographic column, the liquid outlet end of the second chromatographic column is respectively connected with the third chromatographic pump and the sixth port of the second multi-way valve through a tee joint, the first port and the fourth port of the second multi-way valve are connected with two ends of the third chromatographic column, the second port of the second multi-way valve is connected with the fourth chromatographic pump, and the third port of the second multi-way valve is connected with the liquid inlet end of the fourth chromatographic column, and the liquid outlet end of the fourth chromatographic column is connected with the detector, and the fifth port of the second multi-way valve is connected with the second waste liquid pool.
Further, the first chromatography pump, the second chromatography pump, the third chromatography pump and the fourth chromatography pump are binary pumps, quaternary pumps or single pumps.
Further, the detector is an ultraviolet detector, a diode array detector, a fluorescence detector, a single-pole mass spectrometer or a triple quadrupole mass spectrometer.
Adopt above-mentioned technical scheme to have following beneficial effect:
the utility model adopts four chromatographic columns for on-line extraction, improves the impurity removal effect and realizes the whole plasma sample injection detection. The double-column detection can be realized by controlling the pipeline switching of the two multi-way valves, and the detection time, high resolution and high performance of the sample are improved. The first waste liquid pool and the second waste liquid pool realize independent, rapid and automatic exhaust of each pump. Because the liquid is conveyed by the independent pumps, the online extraction and analysis detection speed can be improved, the solvent switching before the pumps is not needed, and the risks of cross contamination of pipeline liquid and the like are avoided.
The following description is further described in conjunction with the accompanying drawings and the detailed description.
Drawings
Fig. 1 is a schematic structural diagram of embodiment 1.
In the drawing, a first multi-way valve a, a second multi-way valve B, a detector D, a first chromatographic pump P1, a second chromatographic pump P2, a third chromatographic pump P3, a fourth chromatographic pump P4, a sample injector SIL, a first chromatographic column C1, a second chromatographic column C2, a third chromatographic column C3, a fourth chromatographic column C4, a first waste liquid tank V1 and a second waste liquid tank V2.
Detailed Description
Specific example 1:
referring to fig. 1, a pump-controlled intelligent multidimensional liquid chromatograph includes a first chromatographic pump P1, a second chromatographic pump P2, a third chromatographic pump P3, a fourth chromatographic pump P4, a sample injector SIL, a first waste liquid tank V1, a second waste liquid tank V2, a detector D, a first chromatographic column C1, a second chromatographic column C2, a third chromatographic column C3, a fourth chromatographic column C4, and a first multi-way valve a and a second multi-way valve B for connecting and switching each pipeline, wherein the first chromatographic pump P1, the second chromatographic pump P2, the third chromatographic pump P3, and the third chromatographic pump P4 are binary pumps, quaternary pumps, or single pumps. A first chromatographic pump P1 is used to deliver a releasing agent for the first chromatographic column C1 on-line extraction column, a second chromatographic pump P2 is used to deliver a mobile phase or releasing agent to the second chromatographic column C2, a third chromatographic pump P3 is used to deliver a mobile phase or releasing agent to the third chromatographic column C3, and a fourth chromatographic pump P4 is used to deliver a mobile phase for on-line analytical testing. The sample injector SIL may be a manual sample injector valve, an autosampler, or a pre-treatment spectrometer, etc. The first chromatographic column C1 is an online solid-phase extraction column for bidirectional separation; the second chromatographic column C2 and the third chromatographic column C3 are online analytical columns or online solid phase extraction columns; the fourth chromatographic column C4 is an on-line analytical column. The first chromatographic column C1, the second chromatographic column C2, the third chromatographic column C3 and the fourth chromatographic column C4 are chromatographic columns with different lengths, pipe diameters and different packing materials. The detector D is an ultraviolet detector UV, a diode array detector DAD, a fluorescence detector, a single-pole mass spectrum MS or a triple quadrupole mass spectrum MS/MS. The first multi-way valve A and the second multi-way valve B can adopt a two-position six-way valve, a three-position seven-way valve, a six-position seven-way valve and the like.
The first chromatographic pump P1 is connected with the input end of the sample injector SIL, the output end of the sample injector SIL is connected with the first multi-way valve A, the second chromatographic pump P2 is connected with the first multi-way valve A, the fourth chromatographic pump P4 is connected with the second multi-way valve B, the first chromatographic column C1 is connected with any two ports of the first multi-way valve A, the liquid inlet end of the second chromatographic column C2 is connected with the first multi-way valve A, and the liquid outlet end of the second chromatographic column C2 is respectively connected with the third chromatographic pump P3 and the second multi-way valve B through a tee joint; the third chromatographic column C3 is connected with any two ports of the second multi-way valve A, the liquid inlet end of the fourth chromatographic column C4 is connected with the second multi-way valve B, and the liquid outlet end of the fourth chromatographic column C4 is connected with the detector D; the first waste liquid pool V1 is connected with the first multi-way valve A, and the second waste liquid pool V2 is connected with the second multi-way valve B.
In this particular embodiment: the first multi-way valve A and the second multi-way valve B are two-position six-way valves, and each two-position six-way valve comprises a first port, a second port, a third port, a fourth port, a fifth port and a sixth port which are sequentially arranged along the anticlockwise direction.
The first port and the fourth port of the first multi-way valve A are connected with two ends of a first chromatographic column C1 through pipelines, the second port of the first multi-way valve A is connected with the output end of the sample injector SIL through a pipeline, the input end of the sample injector SIL is connected with a first chromatographic pump P1 through a pipeline, the third port of the first multi-way valve A is connected with the first waste liquid tank V1 through a waste liquid pipeline, the fifth port of the first multi-way valve A is connected with a second chromatographic pump P2 through a pipeline, the sixth port of the first multi-way valve A is connected with the liquid inlet end of a second chromatographic column C2 through a pipeline, the liquid outlet end of the second chromatographic column C2 is respectively connected with the third chromatographic pump P3 and the sixth port of the second multi-way valve B through a tee joint, the first port and the fourth port of the second multi-way valve B are connected with two ends of a third chromatographic column C3 through pipelines, and a second port of the second multi-way valve B is connected with the fourth chromatographic pump P4 through a pipeline, a third port of the second multi-way valve B is connected with a liquid inlet end of the fourth chromatographic column through a pipeline, a liquid outlet end of the fourth chromatographic column is connected with the detector D through an analysis pipeline, and a fifth port of the second multi-way valve B is connected with the second waste liquid pool V2 through a waste liquid pipeline.
According to the utility model, the connection positions of the ports of the two multi-way valves of A, B can be flexibly switched as required to adjust the dimension of the multi-dimensional liquid chromatograph, and the second chromatographic column C2, the third chromatographic column C3 and the fourth chromatographic column C4 can be independently detected, so that benefit maximization is realized. When a first sample is in the analytical column, the next sample can enter the extraction at this time, which is equivalent to starting the extraction and detection of the next sample without waiting for the first complete detection, thereby improving the working efficiency, and the four chromatographic columns carry out multiple times of extraction and analysis, thereby improving the impurity removal performance, avoiding the pretreatment such as manual protein precipitation, avoiding the operation error, and realizing the full-automatic direct sample introduction detection and the on-line analysis of the whole plasma and the serum.
Claims (5)
1. The utility model provides a pump accuse intelligence multidimension liquid chromatograph, includes injector, first waste liquid pond, second waste liquid pond, detector and is used for connecting and switches the first multi-way valve and the second multi-way valve of each pipeline, its characterized in that: the device comprises a first chromatographic pump, a second chromatographic pump, a third chromatographic pump, a fourth chromatographic pump, a first chromatographic column, a second chromatographic column, a third chromatographic column and a fourth chromatographic column, wherein the first chromatographic pump is connected with the input end of a sample injector, the output end of the sample injector is connected with a first multi-way valve, the second chromatographic pump is connected with a first multi-way valve, the fourth chromatographic pump is connected with a second multi-way valve, the first chromatographic column is connected with any two ports of the first multi-way valve, the liquid inlet end of the second chromatographic column is connected with the first multi-way valve, and the liquid outlet end of the second chromatographic column is respectively connected with the third chromatographic pump and the second multi-way valve through a tee joint; the third chromatographic column is connected with any two ports of the second multi-way valve, the liquid inlet end of the fourth chromatographic column is connected with the second multi-way valve, and the liquid outlet end of the fourth chromatographic column is connected with the detector; the first waste liquid pool is connected with the first multi-way valve, and the second waste liquid pool is connected with the second multi-way valve.
2. The pump-controlled intelligent multi-dimensional liquid chromatograph of claim 1, wherein: the first chromatographic column is an online solid-phase extraction column for bidirectional separation; the second chromatographic column and the third chromatographic column are online analytical columns or online solid-phase extraction columns; the fourth chromatographic column is an online analytical column.
3. The pump-controlled intelligent multi-dimensional liquid chromatograph of claim 1 or 2, wherein: the first multi-way valve and the second multi-way valve are both two-position six-way valves, and each two-position six-way valve comprises a first port, a second port, a third port, a fourth port, a fifth port and a sixth port which are arranged in sequence along the anticlockwise direction;
the first port and the fourth port of the first multi-way valve are connected with two ends of the first chromatographic column, the second port of the first multi-way valve is connected with the output end of the sample injector, the input end of the sample injector is connected with the first chromatographic pump, the third port of the first multi-way valve is connected with the first waste liquid pool, the fifth port of the first multi-way valve is connected with the second chromatographic pump, the sixth port of the first multi-way valve is connected with the liquid inlet end of the second chromatographic column, the liquid outlet end of the second chromatographic column is respectively connected with the third chromatographic pump and the sixth port of the second multi-way valve through a tee joint, the first port and the fourth port of the second multi-way valve are connected with two ends of the third chromatographic column, the second port of the second multi-way valve is connected with the fourth chromatographic pump, and the third port of the second multi-way valve is connected with the liquid inlet end of the fourth chromatographic column, and the liquid outlet end of the fourth chromatographic column is connected with the detector, and the fifth port of the second multi-way valve is connected with the second waste liquid pool.
4. The pump-controlled intelligent multi-dimensional liquid chromatograph of claim 1, wherein: the first chromatographic pump, the second chromatographic pump, the third chromatographic pump and the fourth chromatographic pump are binary pumps, quaternary pumps or single pumps.
5. The pump-controlled intelligent multi-dimensional liquid chromatograph of claim 1, wherein: the detector is an ultraviolet detector, a diode array detector, a fluorescence detector, a single-pole mass spectrum or a triple quadrupole mass spectrum detector.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202122392682.3U CN215894506U (en) | 2021-09-30 | 2021-09-30 | Pump-controlled intelligent multidimensional liquid chromatograph |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202122392682.3U CN215894506U (en) | 2021-09-30 | 2021-09-30 | Pump-controlled intelligent multidimensional liquid chromatograph |
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| Publication Number | Publication Date |
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| CN215894506U true CN215894506U (en) | 2022-02-22 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN202122392682.3U Active CN215894506U (en) | 2021-09-30 | 2021-09-30 | Pump-controlled intelligent multidimensional liquid chromatograph |
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- 2021-09-30 CN CN202122392682.3U patent/CN215894506U/en active Active
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