CN215066380U - On-line solid phase extraction combined two-position liquid chromatograph - Google Patents
On-line solid phase extraction combined two-position liquid chromatograph Download PDFInfo
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- CN215066380U CN215066380U CN202120591867.4U CN202120591867U CN215066380U CN 215066380 U CN215066380 U CN 215066380U CN 202120591867 U CN202120591867 U CN 202120591867U CN 215066380 U CN215066380 U CN 215066380U
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Abstract
The utility model discloses a two-position liquid chromatograph for online solid-phase extraction, including first chromatographic pump, second chromatographic pump, first waste liquid pipeline and analysis pipeline, the liquid outlet of first chromatographic pump is connected with the sample injector, still includes first chromatographic column, second chromatographic column and third chromatographic column, first chromatographic column sets up between the arbitrary both ends mouth of first multi-way valve, the second chromatographic column sets up at the arbitrary both ends mouth of second multi-way valve, the third chromatographic column sets up one of them port of second multi-way valve, the analysis pipeline with third chromatographic column links to each other; the first chromatographic column is used for performing online solid-phase extraction of bidirectional separation, the second chromatographic column is used for online analytical column or online solid-phase extraction, and the third chromatographic column is used for online analysis. The utility model discloses can realize carrying out two detections, improve work efficiency.
Description
Technical Field
The utility model relates to a liquid chromatogram field, in particular to online solid-phase extraction allies oneself with uses two-position liquid chromatograph.
Background
Since macromolecular impurities such as protein contained in the biological sample easily interfere the detection of small molecular compounds and easily cause pipeline blockage and different degrees of deterioration of the first-dimension chromatographic column, the problems can be solved by adopting a multi-dimension liquid chromatography technology. The multidimensional liquid chromatography technology firstly removes protein and other macromolecular impurities through on-line solid phase extraction, enriches small molecular compounds to increase peak capacity, thereby effectively improving the separation degree and peak type of the separation of small molecular compounds in complex samples and improving the sensitivity of the complex samples. However, the existing two-dimensional liquid chromatography technology cannot directly enter a large-volume plasma/serum sample, and the detection precision of a single sample is low.
Disclosure of Invention
The utility model aims at providing a simple structure, improve the online solid-phase extraction of detecting the precision and ally oneself with two-position liquid chromatograph to the not enough of prior art.
The technical scheme of the utility model is that:
the on-line solid-phase extraction combined two-position liquid chromatograph comprises a first chromatographic pump for conveying a releasing agent, a second chromatographic pump for conveying a mobile phase, a first waste liquid pipeline, an analysis pipeline, and a first multi-way valve and a second multi-way valve which are connected in series and used for connecting and switching each pipeline, wherein a liquid outlet of the first chromatographic pump is connected with a sample injector, the first waste liquid pipeline is connected with a first waste liquid pool, the analysis pipeline is provided with a detector, the on-line solid-phase extraction combined two-position liquid chromatograph further comprises three chromatographic columns, namely a first chromatographic column, a second chromatographic column and a third chromatographic column, the first chromatographic column is arranged between any two ports of the first multi-way valve, and the first chromatographic column is used for carrying out on-line solid-phase extraction of two-way separation; the second chromatographic column is arranged at any two ports of the second multi-way valve and is used for on-line analytical column or on-line solid phase extraction; the third chromatographic column is arranged at one port of the second multi-way valve, the analysis pipeline is connected with the third chromatographic column, and the third chromatographic column is used for online analysis.
Furthermore, the first multi-way valve and the second multi-way valve are both two-position six-way valves, a first port of the first multi-way valve is connected with a sample injector of the first chromatographic pump, the first chromatographic column is connected between a second port and a fourth port, a third port is connected with a second port of the second multi-way valve, and a connecting pipe is connected between a fifth port and a sixth port; and a third port of the second multi-way valve is connected with the first waste liquid pipeline, a second chromatographic column is connected between the first port and the fourth port, a fifth port is connected with the third chromatographic column, and a sixth port is connected with the second chromatographic pump.
Furthermore, still be equipped with the flow divider between first chromatographic pump with the injector, be connected with a bypass pipeline on the flow divider, the bypass pipeline pass through the tee bend with the export of injector links to each other.
Furthermore, a drainage pipeline is connected between the analysis pipeline and the waste liquid pipeline through two three-way valves.
And further, the device also comprises a third chromatographic pump for conveying the releasing agent, wherein the third chromatographic pump is connected with the outlet of the sample injector of the first chromatographic pump through a tee joint, and the third chromatographic pump is a binary pump, a quaternary pump or a single pump.
Further, the first chromatographic pump and the second chromatographic pump are both binary pumps, quaternary pumps or single pumps.
Furthermore, the analysis pipeline is provided with a detector, and the detector is an ultraviolet detector, a diode array detector, a single-pole mass spectrum detector or a triple quadrupole mass spectrum detector.
Adopt above-mentioned technical scheme to have following beneficial effect:
the utility model discloses the flow path is simple, adopts three chromatographic column, can realize that the duplex detects through the switching of control multi-way valve, can adjust the quantity through each chromatographic column in a flexible way through the switching of control multi-way valve to realize the chromatogram detection of different dimensions, and can realize the independent quick self-bleeding of each pump, and the check-out time of every sample can improve 8min, has improved work efficiency.
A shunt valve is arranged between the outlet of the first chromatographic pump and the inlet of the sample injector, a bypass pipeline is connected to the shunt valve, and the bypass pipeline is connected with the outlet of the sample injector through a tee joint. The bypass pipeline is additionally arranged, so that the online protein dissociation of the sample in the sample injector can be carried out.
A drainage pipeline is connected between the analysis pipeline and the waste liquid pipeline through two three-way valves, so that single-column mode detection can be performed, and independent detection of the second chromatographic column and the third chromatographic column is realized.
The kit also comprises a third chromatographic pump for conveying a releasing agent, and the dosage and the type of the extraction liquid can be adjusted according to different sample requirements, so that the kit is suitable for more detection items.
The following description is further described in conjunction with the accompanying drawings and the detailed description.
Drawings
Fig. 1 is a schematic structural diagram of specific embodiment 1:
FIG. 2 is a schematic structural view of embodiment 2;
FIG. 3 is a schematic structural view of embodiment 3;
fig. 4 is a schematic structural diagram of embodiment 4.
In the drawing, a first multi-way valve a, a second multi-way valve B, a detector D, a first chromatographic pump P1, a second chromatographic pump P2, a third chromatographic pump P3, a sample injector SIL, a first chromatographic column C1, a second chromatographic column C2, a third chromatographic column C3, a flow dividing valve F, and a first waste liquid pool V1.
Detailed Description
Specific example 1:
referring to fig. 1, an online solid-phase extraction combined two-position liquid chromatograph includes a first chromatographic pump P1 for delivering a releasing agent, a second chromatographic pump P2 for delivering a mobile phase, a first waste liquid pipeline, three chromatographic columns, an analysis pipeline, and two first and second multi-way valves a and B connected in series through a pipeline and used for connecting and switching the pipelines, a liquid outlet of the first chromatographic pump is connected with a sample injector SIL, which can adopt a manual sample injection valve, an autosampler SIL, and a pre-treatment instrument. The analysis pipeline is provided with a detector D, the detector D can be provided with a detector D through an analysis pipeline, and the detector D is an ultraviolet detector UV, a diode array detector DAD, a single-pole mass spectrum MS or a triple quadrupole mass spectrum detector MS/MS and the like. The first waste liquid pipeline is connected with a first waste liquid pool V1, and the first chromatographic pump P1 and the second chromatographic pump P2 are binary pumps, quaternary pumps or single pumps. The three chromatographic columns are respectively a first chromatographic column C1, a second chromatographic column C2 and a third chromatographic column C3, the first chromatographic column C1 is arranged between any two ports of the first multi-way valve A, and the first chromatographic column C1 is used for performing online solid-phase extraction of bidirectional separation; the second chromatographic column C2 is arranged at any two ports of the second multi-way valve B, and the second chromatographic column C2 is used for on-line analytical column or on-line solid phase extraction; the third chromatographic column C3 is arranged at one port of the second multi-way valve (B), the analysis pipeline is connected with the third chromatographic column C3, and the third chromatographic column C3 is used for online analysis.
In this particular embodiment: the first multi-way valve A and the second multi-way valve B are both two-position six-way valves, a first port of the first multi-way valve A is connected with a sample injector SIL of the first chromatographic pump P1, the first chromatographic column C1 is connected between a second port and a fourth port, a third port is connected with a second port of the second multi-way valve, and a connecting pipe is connected between a fifth port and a sixth port; and a third port of the second multi-way valve B is connected with the first waste liquid pipeline, a second chromatographic column C2 is connected between the first port and the fourth port, a fifth port is connected with the third chromatographic column C3, and a sixth port is connected with the second chromatographic pump P2.
Possibly, a first port of the first multi-way valve a is connected to the first waste line and a third port of the second multi-way valve B is connected to the injector SIL of the first chromatographic pump P1.
The utility model discloses can switch A, B three multi-ported valve's port hookup location in a flexible way as required, adopt the twin columns to detect, improve detection efficiency.
Specific example 2:
referring to fig. 2, a shunt valve F is further disposed between the first chromatographic pump P1 and the sample injector SIL, and a bypass line is connected to the shunt valve F and connected to the outlet of the sample injector SIL through a tee. In this embodiment: the flow divider F adopts one to advance two-out (or one advances three-out) structure, can adjust the velocity of flow of each export, be connected with a bypass pipeline on the flow divider F, the bypass pipeline pass through the tee bend with the export of sample injector SIL links to each other, adds the bypass pipeline and can carry out online dilution to the sample in the sample injector, has improved the detection precision. Other features are the same as those of embodiment 1 or embodiment 2, and thus this embodiment is omitted here.
Specific example 3:
referring to fig. 3, the present embodiment is characterized in that: the device also comprises a third chromatographic pump P3 for delivering releasing agents, wherein the third chromatographic pump P3 is connected with the outlet of the sample injector of the first chromatographic pump P1 through a tee joint, and the third chromatographic pump P3 is a binary pump, a quaternary pump or a single pump. The third chromatographic pump is connected with an outlet of the sample injector of the first chromatographic pump through a tee joint, the amount of diluent can be adjusted as required by adopting the third chromatographic pump, online protein dissociation can be carried out on the sample in the sample injector of the first chromatographic pump, and different diluents can be adopted, so that the method is suitable for more detection items. Other features are the same as those of embodiment 1, and thus this embodiment is omitted here.
Specific example 4:
referring to fig. 4, a drainage line is connected between the analysis line and the waste line through two three-way valves. The utility model discloses an on-off of three-way valve control drainage tube realizes the independent detection of second chromatographic column C2 and third chromatographic column. Other features are the same as those of embodiment 1, embodiment 2, or embodiment 3, and thus this embodiment is omitted here.
Claims (7)
1. The utility model provides an online solid-phase extraction allies oneself with uses two-position liquid chromatograph, is used for carrying the first chromatographic pump (P1) of release agent, is used for carrying mobile phase second chromatographic pump (P2), first waste liquid pipeline, analysis pipeline and two first multi-way valve (A) and second multi-way valve (B) that are used for connecting and switch each pipeline of establishing ties, the liquid outlet of first chromatographic pump (P1) is connected with injector (SIL), and first waste liquid pipeline is connected with first waste liquid pond (V1), the analysis pipeline is equipped with detector (D), its characterized in that: the device is characterized by further comprising three chromatographic columns, namely a first chromatographic column (C1), a second chromatographic column (C2) and a third chromatographic column (C3), wherein the first chromatographic column (C1) is arranged between any two ports of the first multi-way valve (A), and the first chromatographic column (C1) is used for an online solid-phase extraction column for bidirectional separation; the second chromatographic column (C2) is arranged at any two ports of the second multi-way valve (B), and the second chromatographic column (C2) is used for on-line analytical column or on-line solid phase extraction; the third chromatographic column (C3) is arranged at one port of the second multi-way valve (B), the analysis pipeline is connected with the third chromatographic column (C3), and the third chromatographic column (C3) is used for on-line analysis.
2. The on-line solid phase extraction in combination with two-position liquid chromatograph of claim 1, wherein: the first multi-way valve (A) and the second multi-way valve (B) are both two-position six-way valves, a first port of the first multi-way valve (A) is connected with a Sample Injector (SIL) of the first chromatographic pump (P1), the first chromatographic column (C1) is connected between a second port and a fourth port, a third port is connected with a second port of the second multi-way valve (B), and a connecting pipe is connected between a fifth port and a sixth port; and a third port of the second multi-way valve (B) is connected with the first waste liquid pipeline, a second chromatographic column (C2) is connected between the first port and the fourth port, a fifth port is connected with the third chromatographic column (C3), and a sixth port is connected with the second chromatographic pump (P2).
3. The on-line solid phase extraction in combination with two-position liquid chromatograph of claim 1, wherein: a shunt valve (F) is further arranged between the first chromatographic pump (P1) and the Sample Injector (SIL), a bypass pipeline is connected to the shunt valve (F), and the bypass pipeline is connected with an outlet of the Sample Injector (SIL) through a tee joint.
4. The on-line solid phase extraction in combination with two-position liquid chromatograph of claim 1, wherein: the device also comprises a third chromatographic pump (P3) for delivering a releasing agent, wherein the third chromatographic pump (P3) is connected with the outlet of the sample injector of the first chromatographic pump (P1) through a tee joint, and the third chromatographic pump (P3) is a binary pump, a quaternary pump or a single pump.
5. The on-line solid phase extraction in combination with two-position liquid chromatograph of claim 1, 3 or 4, wherein: and a drainage pipeline is connected between the analysis pipeline and the waste liquid pipeline through two three-way valves.
6. The on-line solid phase extraction in combination with two-position liquid chromatograph according to claim 1 or 2, wherein: the first chromatography pump (P1) and the second chromatography pump (P2) are binary pumps, quaternary pumps, or single pumps.
7. The on-line solid phase extraction in combination with two-position liquid chromatograph according to claim 1 or 2, wherein: the detector (D) is an ultraviolet detector (UV), a Diode Array Detector (DAD), a single-pole Mass Spectrometer (MS) or a triple quadrupole mass spectrometer (MS/MS).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202120591867.4U CN215066380U (en) | 2021-03-23 | 2021-03-23 | On-line solid phase extraction combined two-position liquid chromatograph |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202120591867.4U CN215066380U (en) | 2021-03-23 | 2021-03-23 | On-line solid phase extraction combined two-position liquid chromatograph |
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| CN215066380U true CN215066380U (en) | 2021-12-07 |
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| CN202120591867.4U Active CN215066380U (en) | 2021-03-23 | 2021-03-23 | On-line solid phase extraction combined two-position liquid chromatograph |
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