CN214427364U - Liquid chromatograph with two columns automatically switched - Google Patents
Liquid chromatograph with two columns automatically switched Download PDFInfo
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- CN214427364U CN214427364U CN202120591870.6U CN202120591870U CN214427364U CN 214427364 U CN214427364 U CN 214427364U CN 202120591870 U CN202120591870 U CN 202120591870U CN 214427364 U CN214427364 U CN 214427364U
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Abstract
The utility model discloses a two-column automatic switching's liquid chromatograph, including the first chromatographic pump that is used for carrying the release agent, be used for carrying mobile phase's second chromatographic pump, first waste liquid pipeline, the analysis pipeline and two first multi-way valve and the second multi-way valve that are used for connecting and switching each pipeline of establishing ties, the liquid outlet of first chromatographic pump is connected with the sample injector, first waste liquid pipeline is connected with first waste liquid pond, the analysis pipeline is equipped with the detector, still include two chromatographic columns, be first chromatographic column and second chromatographic column respectively, first chromatographic column sets up between the arbitrary ports of first multi-way valve, first chromatographic column is used for carrying out the online solid-phase extraction of bidirectional separation; one end of the second chromatographic column is connected with one port of the second multi-way valve, the other end of the second chromatographic column is connected with the analysis pipeline, and the second chromatographic column is used for online analysis. The utility model discloses simple structure has improved the efficiency of independent detection.
Description
Technical Field
The utility model relates to a liquid chromatogram field, in particular to two post automatic switch-over's liquid chromatograph.
Background
Since macromolecular impurities such as protein contained in the biological sample easily interfere the detection of small molecular compounds and easily cause pipeline blockage and different degrees of deterioration of the first-dimension chromatographic column, the problems can be solved by adopting a multi-dimension liquid chromatography technology. The multidimensional liquid chromatography technology firstly removes protein and other macromolecular impurities through on-line solid phase extraction, enriches small molecular compounds to increase peak capacity, thereby effectively improving the separation degree and peak type of the separation of small molecular compounds in complex samples and improving the sensitivity of the complex samples. However, the existing two-dimensional liquid chromatography has a complex structure, cannot be directly used for detecting large-volume plasma/serum samples, and has low working efficiency when a single sample is respectively detected.
Disclosure of Invention
The utility model aims at providing a simple structure, improve detection efficiency's two post automatic switch's liquid chromatograph to prior art not enough.
The technical scheme of the utility model is that:
a liquid chromatograph with two columns capable of automatically switching comprises a first chromatographic pump for conveying a releasing agent, a second chromatographic pump for conveying a mobile phase, a first waste liquid pipeline, an analysis pipeline, and a first multi-way valve and a second multi-way valve which are connected in series and used for connecting and switching pipelines, wherein a liquid outlet of the first chromatographic pump is connected with a sample injector, the first waste liquid pipeline is connected with a first waste liquid pool, the analysis pipeline is provided with a detector, the liquid chromatograph further comprises two chromatographic columns which are respectively a first chromatographic column and a second chromatographic column, the first chromatographic column is arranged between any two ports of the first multi-way valve, and the first chromatographic column is used for online solid-phase extraction of two-way separation; one end of the second chromatographic column is connected with one port of the second multi-way valve, the other end of the second chromatographic column is connected with an analysis pipeline, and the second chromatographic column is used for online analysis.
Furthermore, the first multi-way valve and the second multi-way valve are both two-position six-way valves, the first chromatographic column is connected between the second port and the fourth port of the first multi-way valve, the third port is connected with the second port of the second multi-way valve, the first port is connected with a sample injector of the first chromatographic pump, and the sixth port is connected with the fifth port; and a third port of the second multi-way valve is connected with the first waste liquid pipeline, and a first port is respectively connected with the second chromatographic pump and the second chromatographic column through a tee joint.
Furthermore, a drainage pipeline is connected between the analysis pipeline and the waste liquid pipeline through two three-way valves.
Furthermore, still be equipped with the flow divider between first chromatographic pump with the injector, be connected with a bypass pipeline on the flow divider, the bypass pipeline pass through the tee bend with the export of injector links to each other.
And further, the device also comprises a third chromatographic pump for conveying the releasing agent, wherein the third chromatographic pump is connected with the outlet of the sample injector of the first chromatographic pump through a tee joint, and the third chromatographic pump is a binary pump, a quaternary pump or a single pump.
Further, the first chromatographic pump and the second chromatographic pump are both binary pumps, quaternary pumps or single pumps.
Furthermore, the analysis pipeline is provided with a detector, and the detector is an ultraviolet detector, a diode array detector, a single-pole mass spectrum detector or a triple quadrupole mass spectrum detector.
Adopt above-mentioned technical scheme to have following beneficial effect:
the utility model discloses the flow path is simple, adopts first chromatographic column and second chromatographic column, but the connection port through switching two multi-ported valves of establishing ties is single column fast and is detected, and the check-out time of every sample can improve 8min, has improved work efficiency.
A shunt valve is arranged between the outlet of the first chromatographic pump and the inlet of the sample injector, a bypass pipeline is connected to the shunt valve, and the bypass pipeline is connected with the outlet of the sample injector through a tee joint. The bypass pipeline is additionally arranged, so that the online protein dissociation of the sample in the sample injector can be carried out.
The kit also comprises a third chromatographic pump for conveying a releasing agent, and the dosage and the type of the extraction liquid can be adjusted according to different sample requirements, so that the kit is suitable for more detection items.
The following description is further described in conjunction with the accompanying drawings and the detailed description.
Drawings
Fig. 1 is a schematic structural diagram of specific embodiment 1:
FIG. 2 is a schematic structural view of embodiment 2;
FIG. 3 is a schematic structural view of embodiment 3;
fig. 4 is a schematic structural diagram of embodiment 4.
In the drawing, a first multi-way valve A, a second multi-way valve B, a detector D, a first chromatographic pump P1, a second chromatographic pump P2, a third chromatographic pump P3, a sample injector SIL, a first chromatographic column C1, a second chromatographic column C2, a flow dividing valve F and a first waste liquid pool V1.
Detailed Description
Specific example 1:
referring to fig. 1, a two-column automatic switching liquid chromatograph includes a first chromatographic pump P1 for delivering a releasing agent, a second chromatographic pump P2 for delivering a mobile phase, a first waste liquid pipeline, two chromatographic columns, an analysis pipeline, and two first and second multi-way valves a and B connected in series through a pipeline and used for connecting and switching the pipelines, wherein a liquid outlet of the first chromatographic pump P1 is connected with a sample injector SIL which can adopt a manual sample injection valve, an automatic sample injector SIL, and a pre-treatment instrument. The analysis pipeline is provided with a detector D, the detector D can be provided with a detector D through an analysis pipeline, and the detector D is an ultraviolet detector UV, a diode array detector DAD, a single-pole mass spectrum MS or a triple quadrupole mass spectrum detector MS/MS and the like. The first waste liquid pipeline is connected with a first waste liquid pool V1, and the first chromatographic pump P1 and the second chromatographic pump P2 are binary pumps, quaternary pumps or single pumps. The two chromatographic columns are respectively a first chromatographic column C1 and a second chromatographic column C2, the first chromatographic column C1 is arranged between any two ports of the first multi-way valve A, and the first chromatographic column C1 is used for performing online solid-phase extraction of bidirectional separation; one end of the second chromatographic column C2 is connected with one port of the second multi-way valve B, the other end of the second chromatographic column C2 is connected with an analysis pipeline, and the second chromatographic column C2 is used for online analysis.
In this particular embodiment: the first multi-way valve A and the second multi-way valve B are both two-position six-way valves, the first chromatographic column C1 is connected between the second port and the fourth port of the first multi-way valve A, the third port is connected with the second port of the second multi-way valve, the first port is connected with the sample injector of the first chromatographic pump P1, and the sixth port is connected with the fifth port; and a third port of the second multi-way valve B is connected with a first waste liquid pipeline, and a first port of the second multi-way valve B is respectively connected with the second chromatographic pump P2 and the second chromatographic column C2 through a tee joint.
The utility model discloses can switch A, B three multi-ported valve's port hookup location in a flexible way as required, single-column detection fast.
Specific example 2:
referring to fig. 2, the present embodiment is characterized in that: the first chromatographic pump P1 with still be equipped with flow divider F between the sample injector SIL, be connected with a bypass pipeline on the flow divider F, the bypass pipeline pass through the tee bend with the export of sample injector SIL links to each other. In this embodiment: the flow divider valve F adopts a one-inlet-two-outlet (or one-inlet-three-outlet) structure, the flow rate of each outlet can be adjusted, a bypass pipeline is connected to the flow divider valve F, the bypass pipeline is connected with the outlet of the sample injector SIL through a tee joint, and the sample in the sample injector can be diluted online by adding the bypass pipeline. Other features are the same as those of embodiment 1 or embodiment 2, and thus this embodiment is omitted here.
Specific example 3:
referring to fig. 3, the present embodiment is characterized in that: the device also comprises a third chromatographic pump P3 for delivering releasing agents, wherein the third chromatographic pump P3 is connected with the outlet of the sample injector of the first chromatographic pump P1 through a tee joint, and the third chromatographic pump P3 is a binary pump, a quaternary pump or a single pump. The third chromatographic pump is connected with an outlet of the sample injector of the first chromatographic pump through a tee joint, the amount of diluent can be adjusted as required by adopting the third chromatographic pump, online protein dissociation can be carried out on the sample in the sample injector of the first chromatographic pump, and different diluents can be adopted, so that the method is suitable for more detection items. Other features are the same as those of embodiment 1, and thus this embodiment is omitted here.
Specific example 4:
referring to fig. 4, the present embodiment is characterized in that: a drainage pipeline is connected between the analysis pipeline and the waste liquid pipeline through two three-way valves, so that single-column mode detection can be performed, and independent detection of the second chromatographic column and the third chromatographic column is realized. Other features are the same as those of embodiment 1, embodiment 2, or embodiment 3, and thus this embodiment is omitted here.
Claims (7)
1. The utility model provides a two-column automatic switch-over's liquid chromatograph, is used for carrying the first chromatographic pump (P1) of release agent, is used for carrying mobile phase's second chromatographic pump (P2), first waste liquid pipeline, analysis pipeline and two first multi-way valve (A) and second multi-way valve (B) that are used for connecting and switch each pipeline of establishing ties, the liquid outlet of first chromatographic pump (P1) is connected with injector (SIL), and first waste liquid pipeline is connected with first waste liquid pond (V1), the analysis pipeline is equipped with detector (D), its characterized in that: the device also comprises two chromatographic columns, namely a first chromatographic column (C1) and a second chromatographic column (C2), wherein the first chromatographic column (C1) is arranged between any two ports of the first multi-way valve (A), and the first chromatographic column (C1) is used for carrying out online solid-phase extraction of bidirectional separation; one end of the second chromatographic column (C2) is connected with one port of the second multi-way valve (B), the other end of the second chromatographic column is connected with an analysis pipeline, and the second chromatographic column (C2) is used for on-line analysis.
2. The two-column automatic switching liquid chromatograph of claim 1, wherein: the first multi-way valve (A) and the second multi-way valve (B) are both two-position six-way valves, the first chromatographic column (C1) is connected between the second port and the fourth port of the first multi-way valve (A), the third port is connected with the second port of the second multi-way valve (B), the first port is connected with a sample injector of the first chromatographic pump (P1), and the sixth port is connected with the fifth port through a connecting pipe; and a third port of the second multi-way valve (B) is connected with a first waste liquid pipeline, and a first port of the second multi-way valve (B) is respectively connected with the second chromatographic pump (P2) and the second chromatographic column (C2) through a tee joint.
3. The two-column automatic switching liquid chromatograph of claim 1, wherein: and a drainage pipeline is connected between the analysis pipeline and the waste liquid pipeline through two three-way valves.
4. The two-column automatic switching liquid chromatograph of claim 1 or 3, wherein: a shunt valve (F) is further arranged between the first chromatographic pump (P1) and the Sample Injector (SIL), a bypass pipeline is connected to the shunt valve (F), and the bypass pipeline is connected with an outlet of the Sample Injector (SIL) through a tee joint.
5. The two-column automatic switching liquid chromatograph of claim 1 or 3, wherein: the device also comprises a third chromatographic pump (P3) for delivering a releasing agent, wherein the third chromatographic pump (P3) is connected with the outlet of the sample injector of the first chromatographic pump (P1) through a tee joint, and the third chromatographic pump (P3) is a binary pump, a quaternary pump or a single pump.
6. The two-column automatic switching liquid chromatograph of claim 1, wherein: the first chromatography pump (P1) and the second chromatography pump (P2) are binary pumps, quaternary pumps, or single pumps.
7. The two-column automatic switching liquid chromatograph of claim 1, wherein: the detector (D) is an ultraviolet detector (UV), a Diode Array Detector (DAD), a single-pole Mass Spectrometer (MS) or a triple quadrupole mass spectrometer (MS/MS).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202120591870.6U CN214427364U (en) | 2021-03-23 | 2021-03-23 | Liquid chromatograph with two columns automatically switched |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202120591870.6U CN214427364U (en) | 2021-03-23 | 2021-03-23 | Liquid chromatograph with two columns automatically switched |
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| CN214427364U true CN214427364U (en) | 2021-10-19 |
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| CN202120591870.6U Active CN214427364U (en) | 2021-03-23 | 2021-03-23 | Liquid chromatograph with two columns automatically switched |
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- 2021-03-23 CN CN202120591870.6U patent/CN214427364U/en active Active
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