CN215610825U

CN215610825U - Stirring formula magnetic bead solution mixing mechanism

Info

Publication number: CN215610825U
Application number: CN202121695221.7U
Authority: CN
Inventors: 于京明; 孙旭峰
Original assignee: Shanghai 3D Medicines Co Ltd
Current assignee: Shanghai 3D Medicines Co Ltd
Priority date: 2021-07-23
Filing date: 2021-07-23
Publication date: 2022-01-25
Anticipated expiration: 2031-07-23

Abstract

The utility model relates to a stirring type magnetic bead solution uniformly mixing mechanism, which comprises a container with an opening at the top, an impeller arranged in the container and a container cover for sealing and closing the opening at the top of the container, wherein the magnetic bead solution is fully mixed in a way of stirring the solution by the impeller, the mechanism is high in integration level, small in volume and controlled in vibration characteristic; meanwhile, the mechanism can be independently a functional module, can also be integrated in consumable modules of small-sized equipment such as a card box and a microfluidic chip, is driven by an external rotating motor, can realize the mixing operation of magnetic beads by controlling the rotation rule of the motor, and has the advantages of small occupied space and wide application scene.

Description

Stirring formula magnetic bead solution mixing mechanism

Technical Field

The utility model relates to the technical field of nucleic acid detection experimental instruments, in particular to a stirring type magnetic bead solution uniformly mixing mechanism.

Background

The liquid magnetic beads are widely applied in laboratories, but the application of the liquid magnetic beads to small-sized automation equipment has a plurality of difficulties, such as the magnetic beads are easy to cluster, and the clustering state is more stable and more difficult to beat, disperse and mix uniformly for longer standing time. Therefore, the magnetic bead solution needs to be mixed before use, so as to maximize the magnetic bead effect.

In the prior art, the mixing is performed most often by manual operation in modes of long-time oscillation of oscillation equipment, manual flicking and the like; for the application scene of the small-sized automation equipment, the characteristics of the blending mechanism such as volume, vibration, convenience for integration and the like need to be comprehensively considered, and the commonly used blending structure usually comprises an upper beating device, a lower beating device, a container rotating device and a pulse airflow device.

However, the above-mentioned mixing structures all have significant disadvantages: the mechanism of the upper and lower flapping devices has large volume, needs to occupy large space in equipment, has large motion amplitude frequency, is easy to generate vibration noise, and has the risks of splashing and overflowing of solution; the container rotating device is more limited in application scene due to the need of matching corresponding cover opening and closing structures and liquid transferring mechanisms, and is only applied to large-scale workstations and assembly line equipment generally; the pulse airflow device can introduce a large amount of bubbles in the blending process, and also limits the application of the pulse airflow device, for example, in the extraction of exosome, the integrity of exosome vesicles can be influenced by the explosion of the large amount of bubbles on the liquid level.

SUMMERY OF THE UTILITY MODEL

In order to solve the defects of the prior art, the utility model provides a stirring type magnetic bead solution uniformly mixing mechanism, which can fully mix the magnetic bead solution in a way of stirring the solution by an impeller, and has high mechanism integration level, small volume and controlled vibration characteristic; meanwhile, the mechanism can be independently a functional module, can also be integrated in consumable modules of small-sized equipment such as a card box and a microfluidic chip, is driven by an external rotating motor, can realize the mixing operation of magnetic beads by controlling the rotation rule of the motor, and has the advantages of small occupied space and wide application scene.

In order to achieve the above purpose, the technical scheme adopted by the utility model comprises the following steps:

a stirring type magnetic bead solution uniformly mixing mechanism is characterized by comprising a container with an opening at the top, an impeller assembly arranged in the container and a container cover for sealing and closing the opening at the top of the container;

the container is a shell with a cylindrical inner wall, a first through hole is formed in the bottom of the container, a second through hole is formed in the side wall close to the top opening, and a sealing film which is easy to puncture is covered on the first through hole;

the impeller assembly comprises an impeller rod driven by an external motor to rotate and at least one group of rotary impellers fixedly arranged at the lower part of the impeller rod, the impeller rod penetrates through the center of the container cover through an elastic sealing element and drives the rotary impellers to rotate relative to the container, a contact strip made of flexible materials is arranged at the edge of the rotary impellers, and the contact strip contacts and rubs the inner side wall surface of the container when the rotary impellers rotate relative to the container.

Further, the blending mechanism is used as an independent module for independent use; the second through hole is provided with a filter element which allows the gaseous substance to pass through while organizing the liquid substance to pass through.

Further, the blending mechanism is combined with a card box or a microfluidic chip for use; and the second through hole is provided with a switch valve.

Further, the container cover is provided with a sample adding port which can be opened and sealed.

Further, the rotary impeller comprises two paddles symmetrically arranged by a rotating shaft, or the rotary impeller comprises three or more paddles taking the rotating shaft as a geometric center, and the edges of the paddles are provided with contact strips made of flexible materials.

Further, the paddle that rotatory impeller includes at least one and rises and float the paddle, it sets up to rise to float the paddle slope, just the direction of rotation of rotatory impeller is for following and rises and float paddle inclined plane from lower to upper direction.

The utility model has the beneficial effects that:

by adopting the stirring type magnetic bead solution mixing mechanism, magnetic beads in a gathering state can be dispersed and floated in a mode of stirring the solution by the impeller, so that the magnetic bead solution is fully mixed, the mechanism is simple, the equipment integration level is high, the container is fixed, the mechanism can be better combined with a microfluidic scheme, the mechanism is suitable for being applied to card box consumables of small-sized automatic equipment, the rotary motion is easy to control, and a more controllable mixing effect can be obtained.

Drawings

FIG. 1 is a schematic structural diagram of a stirring type magnetic bead solution mixing mechanism according to a preferred embodiment of the present invention.

Fig. 2 is a schematic structural diagram of the floating blade of the present invention.

Description of the figure numbering: 1-container, 11-first through hole, 12-second through hole, 2-impeller component, 21-impeller rod, 22-rotary impeller, 221-paddle, 222-contact strip, 223-floating paddle, 3-container cover, 31-elastic sealing element and 32-sample loading port.

Detailed Description

For a clearer understanding of the contents of the present invention, reference will be made to the accompanying drawings and examples.

Fig. 1 is a schematic structural diagram of a stirring type magnetic bead solution mixing mechanism according to a preferred embodiment of the present invention, which includes a container 1 with a cylindrical inner wall and an open top, an impeller assembly 2 disposed in the container 1, and a container cover 3 for sealing and closing the open top of the container 1. The round bottom of the container 1 is provided with a first through hole 11 covered by a sealing film, and the side wall close to the top opening is provided with a second through hole 12. When the blending mechanism is used independently, the second through hole 12 is provided with a filter element which allows gaseous substances to pass through and prevents liquid substances from passing through, so that aerosol pollution can be prevented; when the mixing mechanism is used in combination with other equipment, for example, the mixing mechanism is integrated in a card box or a microfluidic chip, the first through hole 11 and the second through hole 12 can be provided with a switch valve which can be independently controlled to be switched on and off according to needs, a filter element is not required to be arranged between the second through hole 12 and the switch valve, the container 1 can be communicated with other microfluidic mechanisms through the first through hole 11 and the second through hole 12 to receive and transfer a magnetic bead solution, and the second through hole 12 can also be used for receiving pressure generated by other mechanisms according to use needs. The sealing film adopts a structure easy to puncture, and can be compatible with a commonly used film breaking mechanism. The impeller assembly 2 is disposed in the container 1, wherein the impeller rod 21 is installed through the center of the container cover 3 by means of the elastic sealing member 31, and the impeller rod 21 is driven to rotate by using an external motor so as to drive the rotary impeller 22 to rotate in the container 1. Necessarily, in order to achieve a better blending effect, the edge of the rotating impeller 22 (the paddle 221 constituting the rotating impeller 22) needs to contact the inner wall surface of the container 1 to reduce the dead angle of the stirring process, and ensure that the magnetic beads adhered to the inner wall of the container 1 can be scraped into the solution; meanwhile, in order to ensure a smooth rotation process, a contact strip 222 made of a flexible material is additionally arranged at the edge position of the rotating impeller 22 (the blades 221 constituting the rotating impeller 22), and is in contact with the inner wall of the container 1 through the contact strip 222.

In the preferred embodiment, the impeller assembly 2 comprises two different sets of upper and lower rotating impellers 22, wherein the rotating impeller 22 closer to the bottom of the vessel 1 employs the floating paddles 223, and the upper rotating impeller 22 employs the vertically disposed paddles 221. The floating paddle 223 adopts an inclined design (as shown in fig. 2), and in the rotating process, the rotating direction of the rotating impeller 22 is ensured to be from bottom to top along the inclined surface of the floating paddle 223, so that the floating paddle 223 can drive the solution to generate strong vertical stirring. Meanwhile, in order to avoid eccentric rotation vibration caused by uneven mass, the rotating impeller 22 adopted by the impeller assembly 2 uses two blades 221 (floating blades 223) symmetrically arranged by the rotating shaft, and of course, according to actual needs, three or more blades 221 (floating blades 223) with the rotating shaft as the geometric center can be adopted, and the effect of uniform rotating mass can be achieved as well.

Preferably, the container cover 3 is provided with an openable, sealed and closed sample adding port 32, so that the mixing mechanism can adapt to more liquid transferring mechanisms and liquid transferring modes, that is, the mixing mechanism can support the mixing of the pre-packaged magnetic bead solution and the mixing operation of adding the magnetic bead solution from the outside by using the liquid transferring mechanism. The sample port 32 can be designed as a one-way opening rotary lid with an automatic reset function and can be matched with a suitable sealing structure, such as a sealing strip.

The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any changes or substitutions that can be easily conceived by those skilled in the art within the technical scope of the present invention are included in the scope of the present invention. Therefore, the protection scope of the present invention shall be subject to the protection scope of the claims.

Claims

1. A stirring type magnetic bead solution uniformly mixing mechanism is characterized by comprising a container with an opening at the top, an impeller assembly arranged in the container and a container cover for sealing and closing the opening at the top of the container;

2. The blending mechanism of claim 1, wherein the blending mechanism is used in combination with a cartridge or microfluidic chip; and the second through hole is provided with a switch valve.

3. The mixing mechanism of claim 2, wherein the container lid is provided with an openable, sealingly closed sample addition port.

4. The blending mechanism of claim 2, wherein the rotary impeller comprises two paddles symmetrically arranged about the rotation axis, or wherein the rotary impeller comprises three or more paddles having the rotation axis as the geometric center, and the edges of the paddles are provided with contact strips made of flexible materials.

5. The blending mechanism according to claim 4, wherein the blades of the rotary impeller include at least one floating blade, the floating blade is arranged in an inclined manner, and the rotation direction of the rotary impeller is from bottom to top along the inclined surface of the floating blade.