CN212833739U

CN212833739U - Bag type biological reaction device

Info

Publication number: CN212833739U
Application number: CN202021182627.0U
Authority: CN
Inventors: 王尧; 陈冲; 徐成荫; 沈陈; 程梦; 肖小波
Original assignee: Shanghai Lepure Biotech Co ltd
Current assignee: Shanghai Lepure Biotech Co ltd
Priority date: 2020-06-23
Filing date: 2020-06-23
Publication date: 2021-03-30
Anticipated expiration: 2030-06-23

Abstract

The utility model relates to a bio-pharmaceuticals technical field especially relates to a bag type biological reaction device. The bag type biological reaction device comprises a bag body, wherein the bag body comprises an inner cavity, a stirring component is arranged in the inner cavity, a first through hole is formed in the bag body, a driving component and a connecting piece capable of penetrating the first through hole are arranged on the outer side of the bag body, and the driving component is connected with the stirring component through the connecting piece; the bag type biological reaction device also comprises a protection component for accommodating the driving component, the protection component is connected with the bag body, and the outer diameter of the protection component is larger than the diameter of the first through hole. The utility model discloses, through special design's drive assembly, avoid the pollution risk that traditional bearing seal transmission probably exists, simplified disposable bag bioreactor's installation, simultaneously along with the scaling of reactor volume, the stirring subassembly can be scaled by the equal proportion.

Description

Bag type biological reaction device

Technical Field

The utility model relates to a bio-pharmaceuticals technical field especially relates to a bag type biological reaction device.

Background

Disposable bioreactors have wide applications in the field of biopharmaceuticals. Conventional reactors have primarily three forms of drive, one agitator (also known as an "impeller") being mechanically connected to a drive shaft or column which descends into the fluid through an opening in the top of the vessel and then causes an external motor to rotate the drive shaft or column. In closed systems, the agitator is typically connected to an external motor by a hydraulically sealed drive shaft. However, these types of agitation devices are generally not practical for mixers and bioreactors used to manufacture pharmaceuticals or biological materials because the fluid in the vessel may become contaminated and may leak.

Secondly, coupling the agitator inside the vessel to the drive system or motor outside the mixer or bioreactor in a magnetically coupled manner eliminates contamination problems, allows for a completely closed system, and prevents leakage. The magnetically coupled system may eliminate the need to seal between the drive shaft and the vessel because the drive shaft need not penetrate the bag support structure wall to mechanically rotate the agitator. Most magnetic agitator systems include a rotating magnetic drive head outside the vessel and a rotating magnetic agitator within the vessel. The movement of the magnetic drive head enables the transmission of torque and thus the rotation of the magnetic agitator, allowing the agitator to mix the contents of the vessel. This form is most widely used in disposable bioreactors. But has a plurality of disadvantages that firstly, the driving is arranged on the supporting container of the reactor, and the matched disposable bioreactor bag is inconvenient to install; secondly, the magnetic coupling transmission mode is limited in rotating speed, and the phenomenon of demagnetization and the like is easily caused when the rotating speed is too high; the stirring component driven by magnetic coupling can not be scaled with the change of the reactor volume, and has some limitations to some extent and higher processing cost.

The third one is magnetic suspension technology, which utilizes the repulsion force of magnetic field polarity and magnetic rotor to drive the rotating device. This type of form has many drawbacks, and first, the stirring subassembly can only be in the bottom region of reactor bag, if reactor bag volume grow, when highly very high, the phenomenon that the mixing is insufficient can appear. Secondly, the stirring subassembly is not fixed in the axial, and in the actual transportation process, the phenomenon that the dislocation drops appears in the stirring subassembly easily, has very big risk.

SUMMERY OF THE UTILITY MODEL

In view of the above shortcomings of the prior art, it is an object of the present invention to provide a bag bioreactor to solve the problems of contamination risk and limitation to a fixed support container that may exist in the conventional shaft seal transmission in the prior art.

In order to achieve the above and other related objects, the present invention relates to a bag type bioreactor, which comprises a bag body, wherein the bag body comprises an inner cavity, a stirring component is arranged in the inner cavity, a first through hole is arranged on the bag body, the bag type bioreactor further comprises a driving component arranged outside the inner cavity of the bag body and a connecting member capable of penetrating the first through hole, and the driving component is connected with the stirring component through the connecting member; the bag type biological reaction device also comprises a protection component used for accommodating and coating the driving component, the protection component is connected with the bag body, and the outer diameter of the protection component is larger than the diameter of the first through hole.

In some embodiments of the present invention, the stirring assembly includes a stirring shaft and stirring blades disposed on the stirring shaft, and the stirring shaft is connected to the connecting member.

In some embodiments of the present invention, the stirring shaft is provided with a plurality of stirring blades.

In some embodiments of the present invention, the connecting member is a cylinder, and the inner diameter of the connecting member is matched with the outer diameter of the stirring shaft.

In some embodiments of the present invention, the protection assembly includes a driving accommodating portion and a cover body covering the driving accommodating portion, and the cover body is located at one end far away from the bag body; the drive accommodating part is fixedly connected with the bag body.

In some embodiments of the present invention, one side of the cover body away from the driving accommodating portion is provided with a second through hole, and the connecting portion of the driving assembly penetrates through the second through hole and the connecting member.

In some embodiments of the invention, the outer diameter of the connecting member matches the diameter of the second through hole.

The utility model discloses an in some embodiments, the one side that drive assembly was kept away from to the bag body is equipped with the third through-hole, bag-type biological reaction device still include with third through-hole matched with chassis, the chassis through the third through-hole with the one end that drive assembly was kept away from to the stirring subassembly is connected.

In some embodiments of the present invention, the stirring assembly is connected to the chassis through a third through hole penetrated by the bearing assembly.

In some embodiments of the present invention, a ventilation assembly is disposed between the stirring assembly and the chassis.

The utility model has the advantages that:

the utility model discloses an among the pocket type biological reaction device, drive assembly through special design, adopt the complete cladding drive assembly of protection subassembly, and fix the protection subassembly on the bag body, it is sealed better to have the effect, avoid the pollution risk that traditional bearing seal transmission probably exists, the installation of disposable bag bioreactor has been simplified, simultaneously along with the zooming of reactor volume, the stirring subassembly can be scaled by the equal proportion, disposable bioreactor no longer limits fixed support container, various types of reactor support container can be matchd to this drive design. In addition, the driving assembly can be used for one time when in use, and can also be detached and recycled after use. Compared with a magnetic coupling transmission and magnetic suspension structure, the magnetic suspension bag has the advantages that inconvenient factors of installation between the bag body and the supporting container are eliminated to a great extent, the process is simple, the compatibility is wide, the cost is low, and the energy is saved.

Drawings

Fig. 1 is a schematic front view of a bag type biological reaction device according to a first embodiment of the present invention.

Fig. 2 is a schematic front view of a bag type biological reaction device according to a second embodiment of the present invention.

Fig. 3 is an exploded perspective structural view of a bag type biological reaction device according to a third embodiment of the present invention.

Fig. 4 is a schematic front view of a bag type biological reaction device according to a third embodiment of the present invention.

The utility model discloses the component label in the figure:

1 bag body

101 lumen

2 stirring assembly

201 stirring shaft

202 stirring blade

3 drive assembly

4 connecting piece

5 first through hole

6 protection assembly

601 drive receiving part

602 cover

7 second through hole

8 bottom plate

9 third through hole

10 bearing assembly

11 vent assembly

Detailed Description

In the description of the present invention, it should be noted that the structure, ratio, size, etc. shown in the attached drawings of the present specification are only used for matching with the content disclosed in the specification, so as to be known and read by the people familiar with the technology, and are not used for limiting the limit conditions that the present invention can be implemented, so that the present invention does not have the substantial technical significance, and the modification of any structure, the change of the ratio relationship or the adjustment of the size should still fall within the range that the technical content disclosed in the present invention can cover without affecting the function that the present invention can produce and the purpose that can be achieved. While the terms "central," "longitudinal," "lateral," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," and the like refer to orientations or positional relationships illustrated in the drawings, which are used for convenience in describing the invention and to simplify the description, and do not indicate or imply that the referenced device or element must have a particular orientation, be constructed and operated in a particular orientation, and thus, are not to be construed as limiting the invention. Furthermore, the terms "first" and "second" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.

In the description of the present invention, it is to be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "connected," and "connected" are to be construed broadly, and may be, for example, fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meaning of the above terms in the present invention can be understood according to specific situations by those skilled in the art.

In addition, in the description of the present invention, "a plurality" means two or more unless otherwise specified.

As shown in fig. 1 and 2, the first embodiment and the second embodiment of the present invention provide a bag type bioreactor, the bag type bioreactor comprises a bag body 1, the bag body 1 comprises an inner cavity 101, a stirring component 2 is arranged in the inner cavity 101, a first through hole 5 is arranged on the bag body 1, the bag type bioreactor further comprises a driving component 3 arranged outside the inner cavity of the bag body 1 and a connecting component 4 capable of penetrating the first through hole 5, the driving component 3 is connected with the stirring component 2 through the connecting component 4, and the reaction material in the inner cavity 101 can be stirred under the driving of the driving component 3; the bag type biological reaction device also comprises a protection component 6 used for containing and coating the driving component 3, the protection component 6 is connected with the bag body 1, and the outer diameter of the protection component 6 is larger than the diameter of the first through hole 5, so that the first through hole 5 can be well sealed. Referring to fig. 1, the first through hole 5 in the first embodiment is disposed at the top of the inner cavity 101 of the bag body 1, for example, at the top center of the inner cavity 101 of the bag body 1. The height of the driving component 3 is higher than the top of the bag body 1. Referring to fig. 2, the first through hole 5 in the second embodiment is disposed at the bottom of the inner cavity 101 of the bag body 1, for example, at the center of the bottom of the inner cavity 101 of the bag body 1. The height of the driving component 3 is lower than the bottom of the inner cavity 101 of the bag body 1.

The utility model provides a bag type bioreactor which is a disposable bag type bioreactor. Drive assembly 3 directly is connected with stirring subassembly 2 through connecting piece 4, and fixes on the bag body 1 through protection component 6, and the sealed effect of drive assembly 3 than traditional bearing seal (bearing) of being totally wrapped up by protection component 6 is better, has avoided traditional bearing seal transmission to probably introduce the inside pollution of the bag body 1. And the connecting piece can also play a role in auxiliary sealing. The design is not limited to the position of the driving assembly 3 nor to the form of the stirring assembly 2. Because the traditional driving device is arranged on the supporting container, the driving component 3 of the invention is directly connected with the bag body 1, the installation process of the disposable bioreactor bag is simplified, simultaneously, along with the scaling of the reactor volume, the stirring component 2 can be scaled in equal proportion, the disposable bioreactor is not limited to the fixed supporting container any more, and the driving design can be matched with various types of reactor supporting containers.

The utility model provides an among the bag-type biological reaction device, stirring subassembly 2 includes (mixing) shaft 201 and locates stirring paddle leaf 202 on the (mixing) shaft 201, the (mixing) shaft 201 with connecting piece 4 is connected. The shape of the stirring blade 202 is not particularly limited, and it is generally the case that at least two blades are divergently distributed outward along the center of the stirring shaft 201 for stirring the reaction material. In general, the number of the stirring blades 202 distributed on the stirring shaft 201 is not particularly limited, and one or more stirring blades 202 may be selected according to the actual application.

The utility model provides an among the bag-type biological reaction device, under the general condition, in order to stir fully, can extend (mixing) shaft 201 from first through-hole 5 ends to the bottom of the 1 inner chamber 101 of the bag body along its axial direction. A plurality of stirring blades 202 are uniformly arranged on the stirring shaft 201 respectively, so that the stirring is more uniform.

The utility model provides an among the bag-type biological reaction device, the internal diameter of connecting piece 4 cooperatees with the external diameter of (mixing) shaft 201. Here, fitting may mean, for example, that the inner diameter of connecting element 4 is equal to the outer diameter of stirring shaft 201, or that the inner diameter of connecting element 4 is slightly smaller than the outer diameter of stirring shaft 201, so that connecting element 4 and stirring shaft 201 can be well fixed. In general, the choice of the connecting member 4 is not particularly limited, and the stirring shaft 201 and the driving assembly 3 may be connected as they are. In a preferred embodiment, the connecting member 4 is selected from a guide sleeve, the guide sleeve is a hollow cylinder matched with the driving component and the stirring shaft, and the material of the guide sleeve can be, for example, a stainless steel structure.

The utility model provides an among the pocket type biological reaction device, protection component 6 includes that drive receiving portion 601 and lid fit the lid 602 of drive receiving portion 601, lid 602 is located the one end of keeping away from the bag body 1. In general, the shape of the cavity of the drive receiving portion 601 matches the shape of the drive component 3, so that the drive component 3 can be received in the drive receiving portion 601 without being particularly shaken. The design of lid 602 can make things convenient for drive assembly 3 and stirring subassembly 2's dismantlement for drive assembly 3 and the separation of bag body 1, drive assembly 3 can repetitious usage. In one embodiment, the protection component 6 is fixedly connected to the bag body 1, for example, the protection component 6 can be directly welded around the first through hole 5 on the bag body 1. More specifically, the side of the driving accommodating portion 601 away from the cover 602 is fixedly connected to the bag body 1, for example, the side of the driving accommodating portion 601 away from the cover 602 can be directly welded around the first through hole 5 on the bag body 1.

The utility model provides an among the pocket type biological reaction device, one side that lid 602 was kept away from to drive portion 601 is equipped with second through-hole 7, drive assembly 3's connecting portion are worn to penetrate second through-hole 7 is connected with connecting piece 4. The connecting part of the driving assembly 3 is a part connected with the connecting piece 4, the outer diameter of the connecting part of the driving assembly 3 is matched with the inner diameter of the connecting piece 4, specifically, the outer diameter of the connecting part of the driving assembly 3 is equal to the inner diameter of the connecting piece 4 or the outer diameter of the connecting part of the driving assembly 3 is slightly larger than the inner diameter of the connecting piece 4, so that the driving assembly and the connecting piece are well fixed. In a specific embodiment, the outer diameter of the connecting piece 4 matches the diameter of the second through hole 7. The matching means that the outer diameter of the connecting member 4 is equal to the diameter of the second through hole 7 or the outer diameter of the connecting member 4 is slightly smaller than the diameter of the second through hole 7, so that the connecting member 4 and the second through hole 7 can be well fixed, but the rotation of the connecting member 4 along with the driving assembly 3 is not affected. In general, the choice of the connecting member 4 is not particularly limited, and the stirring shaft 201 and the driving assembly 3 may be connected as they are.

The third embodiment of the present invention provides a bag type bioreactor, which comprises, in addition to the above technical solution of the first embodiment, a third through hole 9 disposed on a side of the bag body 1 away from the driving component 3, and a chassis 8 matched with the third through hole 9, wherein the chassis 8 is connected to one end of the stirring component 2 away from the driving component 3 through the third through hole 9. More specifically, the base plate 8 is connected with one end of the stirring shaft 201 far away from the driving assembly 3 through a third through hole 9. This allows the stirring shaft 201 of the stirring assembly 2 to be fixed in the inner cavity 101 of the bag body 1. In a specific embodiment, a plurality of stirring blades 202 are uniformly distributed on the stirring shaft 201.

The utility model provides an among the bag-type biological reaction device, stirring subassembly 2 wears to penetrate third through hole 9 through bearing assembly 10 and is connected with chassis 8. The bearing assembly 10 may be located, for example, within the third through-hole 9. The bearing assembly 10 includes a bearing and a bearing housing that houses the bearing. For securing the stirring assembly 2 to the base plate 8.

The utility model provides an among the bag-type biological reaction device, be equipped with subassembly 11 of ventilating between stirring subassembly 2 and the chassis 8. The venting assembly 11 may be, for example, one or more venting sheets that may be used for venting. The ventilation sheet can be, for example, a solid PE cylinder with a diameter of 2.8-3.2 cm and a height of 2.8-3.2 cm, and is provided with a through hole along the height direction, and the aperture of the through hole is 0.2-0.8 mm. The vent sheet may also be a PE sintered sheet-like microporous structure. The vent sheet may also be a hollow cylinder with perforations along the side wall of the cylinder. In a particular embodiment, the breather assembly 11 may be disposed between the bearing and the bearing housing.

In the bag-type bioreactor provided by the present invention, in each of the aforementioned embodiments, the driving component 3 may be a motor, for example.

The utility model provides an among the bag type biological reaction device, in aforementioned each embodiment, bag type biological reaction device is the same with general biological reaction device, is equipped with feed inlet and discharge gate (not drawn in the figure), conveniently reacts the material discharge after feeding and the reaction.

In general, the bag-type bioreactor of the present invention needs to be used in conjunction with a supporting container for supporting the bag body 1 because the bag body 1 is made of a soft material. When in use, the bag body 1 is placed in a supporting container, like the bag type biological reaction device of the first embodiment, the supporting container is provided with the accommodating parts of the driving component 3 and the motor component, so that the driving component 3 is conveniently fixed. As in the case of the bag type bioreactor of the second embodiment, the bottom of the support container is provided with a recess for receiving and fixing the driving unit 3. In the case of the bag type bioreactor according to the third embodiment, the driving unit 3 and the receiving portion of the protection unit 6 are disposed above the support container, so that the driving unit 3 can be conveniently fixed. The bottom of the support container is provided with a recess for receiving and securing the chassis 8.

The specific using process is as follows:

after being fixed in supporting container with bag type biological reaction device, put into bag body 1 inner chamber 101 with reaction material through the feed inlet, open drive assembly 3, for example the motor, drive assembly 3 drive stirring subassembly 2's stirring thick liquid rotates, can stir reaction material through drive assembly 3 control stirring speed, and the exhaust from the discharge gate is discharged after the reaction is accomplished to the accessible subassembly 11 of ventilating during the period.

To sum up, the utility model discloses an among the pocket type bioreactor, drive assembly 3 through special design, adopt the complete cladding drive assembly of protection subassembly 6, and with the fixed (for example welding) of protection subassembly 6 on the bag body, sealed effect is better, avoid the pollution risk that traditional bearing seal transmission probably exists, the installation of disposable pocket type bioreactor has been simplified, simultaneously along with the scaling of reactor volume, stirring subassembly 2 can scale by the equal proportion, disposable bioreactor no longer limits fixed supporting container, this drive design can match various types of reactor supporting container. In addition, the driving assembly 3 can be used for one time when in use, and can also be detached and recycled after use. Compared with a magnetic coupling transmission and magnetic suspension structure, the magnetic suspension bag has the advantages that inconvenient factors of installation between the bag body 1 and the supporting container are greatly omitted, the process is simple, the compatibility is wide, the cost is low, and the energy is saved.

To sum up, the utility model effectively overcomes various defects in the prior art and has high industrial utilization value.

The above embodiments are merely illustrative of the principles and effects of the present invention, and are not to be construed as limiting the invention. Modifications and variations can be made to the above-described embodiments by those skilled in the art without departing from the spirit and scope of the present invention. Accordingly, it is intended that all equivalent modifications or changes which may be made by those skilled in the art without departing from the spirit and technical spirit of the present invention be covered by the claims of the present invention.

Claims

1. The bag type biological reaction device is characterized by comprising a bag body (1), wherein the bag body (1) comprises an inner cavity (101), a stirring component (2) is arranged in the inner cavity (101), a first through hole (5) is formed in the bag body (1), the bag type biological reaction device further comprises a driving component (3) arranged on the outer side of the inner cavity of the bag body (1) and a connecting piece (4) capable of penetrating the first through hole (5), and the driving component (3) is connected with the stirring component (2) through the connecting piece (4); the bag type biological reaction device also comprises a protection component (6) used for containing and covering the driving component (3), wherein the protection component (6) is connected with the bag body (1), and the outer diameter of the protection component (6) is larger than the diameter of the first through hole (5).

2. The bag bioreactor apparatus according to claim 1, wherein the stirring assembly (2) comprises a stirring shaft (201) and stirring blades (202) disposed on the stirring shaft (201), and the stirring shaft (201) is connected to the connecting member (4).

3. The bag bioreactor apparatus according to claim 2, wherein the stirring shaft (201) is provided with a plurality of stirring blades (202).

4. The bag bioreactor apparatus according to claim 2, wherein the connector is a cylinder, and the inner diameter of the connector (4) is matched to the outer diameter of the stirring shaft (201).

5. The bag-type bioreaction device according to claim 1, wherein the protection assembly (6) comprises a drive container (601) and a cover body (602) covering the drive container (601), the cover body (602) being located at one end away from the bag body (1); the drive accommodating part (601) is fixedly connected with the bag body (1).

6. The bag-type bioreactor apparatus according to claim 5, wherein a second through hole (7) is provided on a side of the driving container (601) away from the cover (602), and the connecting portion of the driving assembly (3) penetrates the second through hole (7) to connect with the connecting member (4).

7. The bag bioreactor apparatus according to claim 6, wherein the connector (4) has an outer diameter matching the diameter of the second through hole (7).

8. The bag-type bioreaction device according to any one of claims 1-7, wherein a third through hole (9) is formed in the side of the bag body (1) away from the driving component (3), and the bag-type bioreaction device further comprises a bottom plate (8) matched with the third through hole (9), and the bottom plate (8) is connected with one end of the stirring component (2) away from the driving component (3) through the third through hole (9).

9. The bag bioreactor apparatus according to claim 8, wherein the stirring assembly (2) is connected to the bottom plate (8) by a bearing assembly (10) penetrating the third through hole (9).

10. The bag bioreactor apparatus according to claim 8, wherein a ventilation assembly (11) is provided between the stirring assembly (2) and the bottom plate (8).