CN219363663U - Lower magnetic stirring type bioreactor - Google Patents

Abstract

The utility model discloses a lower magnetic stirring type bioreactor, a liquid storage container and a ventilation disk arranged at the lower end, which aims to solve the problem of insufficient sealing effect of a driving mode in the field of cell culture or bio-pharmaceuticals; the first pipe body is arranged in the liquid storage container, and the lower end of the first pipe body is connected with the ventilation disc; the installation shell is connected with the upper end of the first pipe body; the first magnetic force rotating piece is arranged in the installation shell; the middle part of the driving shaft is arranged in the first pipe body, and the upper end of the driving shaft is inserted into the mounting shell and connected with the first magnetic force rotating piece; the second magnetic force rotating piece is sleeved on the installation shell and provided with stirring paddles. The mode of driving the second magnetic force rotating part through the magnetic force of the first magnetic force rotating part avoids the problem of insufficient sealing effect existing in the existing mechanical driving mode, is convenient to apply to small and medium-capacity bioreactors, can enlarge the stirring range, and is convenient to install parts such as a first pipe body at the position of the ventilation disk.

Description

Lower magnetic stirring type bioreactor

Technical Field

The utility model relates to the field of cell culture or bio-pharmaceuticals, in particular to a lower magnetic stirring type bioreactor.

Background

Cell culture refers to a method of simulating in vitro an in vivo environment to survive, grow, reproduce and maintain primary structure and function.

The bioreactor is a production experimental device for providing a reaction environment for cell culture, pharmaceutical production, animal and plant tissue culture, microbial fermentation production, biological product preparation, sewage treatment and other experiments, and is sometimes also called a fermenter or reactor.

With the continuous development of cell culture technology, the wide application of bioreactors has been a major trend in the fields of cell culture and pharmacy, and with the continuous improvement of requirements for safety, convenience, high efficiency and the like of cell culture processes in the fields, the application of disposable systems has been over 30 years old, in China, the application of disposable systems is relatively late in starting, but has developed rapidly in recent years, a batch of biopharmaceutical enterprises have introduced disposable systems into pharmaceutical processes, and at present, the disposable systems in the pharmaceutical fields have been widely applied to the processes of cell culture/fermentation, preparation and storage of culture media and buffer solutions, and to the processes of storage and transfer of process materials, freeze thawing of final products, raw solutions and semi-finished products, filling, sampling and the like.

Disposable bioreactors are often used in disposable systems, and most of the containers of disposable bioreactors are bags, and most of the containers in the market have a capacity of 3L to 2000L, and also have reactors with larger capacities, such as 6000L.

At present, the general disposable bioreactor generally adopts a stirring assembly to realize the sealing between the parts of the stirring assembly and the container in a mechanical sealing mode, and the sealing mode easily causes leakage of culture solution in the container, so that the normal use of the reactor is affected, and the influence of cultured cells on the outside, such as bacteria contamination risk, cannot be effectively controlled.

Disclosure of Invention

The utility model provides a lower magnetic stirring type bioreactor, which aims at solving at least one of the technical problems existing in the prior art

The present utility model provides a lower magnetic stirring bioreactor comprising:

the lower end of the liquid storage container is provided with a ventilation disc;

the first pipe body is arranged in the liquid storage container, and the lower end of the first pipe body is connected with the ventilation disc;

the installation shell is connected with the upper end of the first pipe body;

the first magnetic force rotating piece is arranged in the installation shell;

the middle part of the driving shaft is arranged in the first pipe body, and the upper end of the driving shaft is inserted into the mounting shell and connected with the first magnetic force rotating piece;

the second magnetic force rotating piece is sleeved on the installation shell and provided with stirring paddles;

the driving shaft rotates to drive the first magnetic rotating piece to rotate, and the first magnetic rotating piece rotates to drive the second magnetic rotating piece to rotate.

Further, the lower magnetic stirring type bioreactor further comprises:

the middle part of the driving shaft is arranged in the second pipe body;

the lower end of the second pipe body is connected with the ventilation disk in a threaded manner, and the upper end of the second pipe body is connected with the installation shell in a threaded manner;

the first pipe body is a silicone pipe, and the second pipe body is a stainless steel pipe.

Further, the lower magnetic stirring type bioreactor further comprises:

the mounting plate is arranged below the liquid storage container, the lower end of the second pipe body is connected with the mounting plate in a threaded manner, and the upper end of the second pipe body is connected with the mounting shell in a threaded manner;

the driving piece is positioned below the liquid storage container, the lower end of the driving shaft penetrates through the mounting plate and is connected with the driving piece, and the driving piece can drive the driving shaft to rotate;

the lower end of the driving shaft is provided with a connecting sleeve, and the output end of the driving piece is connected with the connecting sleeve in a key connection mode.

Further, the lower extreme interference connection vent disc of first body.

Further, the first tube body is a silicone tube;

the lower extreme of installation casing includes installation tube portion, and the upper end of installation tube portion connection silicone tube, second magnetic force rotate the piece cover and locate on the installation tube portion.

Further, the lower magnetic stirring type bioreactor further comprises:

the upper end of the middle connecting pipe is connected with the mounting pipe part, and the second magnetic force rotating piece is supported by the middle connecting pipe.

Further, the lower end of the middle connecting pipe is provided with an annular convex buckle, and the annular convex buckle can be buckled into the upper end of the silicone tube;

the upper end of the middle connecting pipe is connected with the installation pipe part in a threaded mode, and a sealing ring is arranged between the upper end of the middle connecting pipe and the installation pipe part.

Further, the first magnetic rotator includes:

a first rotary housing connected to the drive shaft;

the first magnetic piece is arranged in the first rotating shell;

the lower end of the first rotating shell is connected with the inner wall of the lower end of the mounting shell in a bearing manner;

the upper end of the first rotating shell is connected with the inner wall of the upper end of the mounting shell in a bearing mode.

Further, the second magnetic rotator includes:

the second rotating shell is sleeved on the mounting shell;

the second magnetic piece is arranged in the second rotary shell;

the second rotating shell is connected with the mounting shell through a bearing mode.

Further, the lower end of the driving shaft extends to the lower side of the vent disc, and the lower end of the driving shaft is connected with the outer wall of the vent disc in a bearing mode.

The beneficial effects of the utility model are as follows:

according to the lower magnetic stirring type bioreactor, the second magnetic rotating part is driven by the magnetic force of the first magnetic rotating part, and the power part and the transmission part of the stirring part are isolated from the space in the liquid storage container better, so that the problem of insufficient sealing effect in the existing mechanical driving mode is avoided;

the installation mode of the magnetic stirring assembly is convenient, a traditional through type driving shaft is omitted, the liquid storage container is convenient to fold, packaging, transportation and field installation of the bioreactor are facilitated, the driving shaft is installed outside the inner space of the liquid storage container, the materials inside and outside the storage container can be isolated from being made of metal-free materials, negative influences of metal on the inside of the bioreactor are avoided, and the bioreactor is easier to realize a sterile environment;

the bioreactor is configured in a lower magnetic stirring mode, so that the bioreactor is conveniently applied to bioreactors with various capacities, a magnetic stirring structure is arranged at other positions relative to the liquid level of the culture solution in the liquid storage container from bottom to top, the stirring range, especially the vertical stirring range, can be enlarged by the lower magnetic stirring mode, and the position of the ventilation disc is convenient for installing components such as the first tube body.

Drawings

FIG. 1 is a schematic structural view of an embodiment of a bioreactor;

FIG. 2 is a schematic structural view of a magnetic stirring assembly embodiment at a cross-sectional view 1;

FIG. 3 is a schematic structural view of a magnetic stirring assembly embodiment at a cross-sectional view 2;

fig. 4 is an enlarged schematic view of the portion a in fig. 3.

Reference numerals: the liquid storage container 100, the vent disc 110, the first pipe body 210, the mounting case 300, the mounting pipe portion 310, the first magnetic force rotating member 400, the driving shaft 510, the connecting sleeve 520, the second magnetic force rotating member 600, the stirring blade 700, the second pipe body 800, the intermediate connecting pipe 910, and the annular protruding buckle 920.

Detailed Description

The following description will describe several embodiments of the present utility model, including the embodiments corresponding to the accompanying drawings, it being understood that the drawings are for aiding in the understanding of the technical features and technical solutions of the present utility model, and should not be construed as limiting the scope of the present utility model.

The conception, specific structure, and technical effects produced by the present utility model will be clearly and completely described below with reference to the embodiments and the drawings to fully understand the objects, aspects, and effects of the present utility model. It should be noted that, in the case of no conflict, the embodiments and features in the embodiments may be combined with each other.

It should be noted that, unless explicitly defined otherwise, when a certain feature is referred to as being "fixed," "connected," or "mounted" on another feature, it may be directly fixed or connected to the other feature or may be indirectly fixed or connected to the other feature, and the terms such as "fixed," "connected," or "mounted" should be interpreted broadly, so that those skilled in the art can reasonably determine the specific meaning of the terms in the present utility model in combination with the specific contents of the technical scheme.

It should be noted that, the description of the orientation or positional relationship indicated by the upper, lower, left, right, top, bottom, front, rear, inner, outer, etc. used in the present utility model is based on the orientation or positional relationship of the drawings or the embodiments, only for convenience of describing the present utility model and simplifying the description, and does not indicate or imply that the apparatus or elements referred to must have a specific orientation, be configured and operated in the specific orientation, and thus should not be construed as limiting the present utility model.

It is noted that the term "and/or" as used in the present utility model includes any combination of one or more of the listed items, meaning one or more, meaning at least two, greater than, less than, exceeding, etc. are understood to exclude this number, and the above, below, within, etc. are understood to include this number.

The rotation conversion rotating member defined in the utility model can rotate around the axis of the rotation conversion rotating member and can be driven to rotate by other power members or transmission members.

It should be noted that, if the first and second descriptions are only used for distinguishing technical features in the present utility model, the description should not be construed as indicating or implying relative importance or implying the number of the indicated technical features or implying the precedence relationship of the indicated technical features.

It is to be understood that all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art unless explicitly defined otherwise. The terminology used in the description presented herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the utility model.

The basic embodiment of the present utility model provides a lower magnetic stirring bioreactor, referring to fig. 1-4, comprising a liquid storage container 100 and a magnetic stirring assembly; the magnetic stirring assembly includes:

the first pipe body 210 is arranged in the liquid storage container 100, the lower end of the first pipe body 210 is connected with the vent disc 110 at the lower end of the liquid storage container 100, further, the lower end of the first pipe body 210 can be connected with the vent disc 110 in a sealing way, both the first pipe body 210 and the liquid storage container 100 can be provided with deformable structures, wherein the liquid storage container 100 can be formed by adopting high-barrier multilayer co-extrusion films meeting pharmaceutical standards through heat sealing, the liquid storage container 100 can be folded for transportation and installation, the vent disc 110 is a part used for connecting an external air inlet or exhaust pipe group in the bioreactor, a plurality of vent interfaces can be integrated on the vent disc 110, the vent disc 110 can be arranged at the bottom of the lower end of the liquid storage container 100, and the outer edge of the vent disc 110 is connected with the lower end wall surface of the liquid storage container 100 in a sealing way, in particular in a hot melting interference way or an ultrasonic welding way;

the installation housing 300 is connected to the upper end of the first pipe body 210, the installation housing 300 is used for protecting the first magnetic rotating member 400, and further, the installation housing 300 may include two parts, one part is used for internally installing the first magnetic rotating member 400, and the other part is used for connecting the first pipe body 210 and installing the second magnetic rotating member 600;

the first magnetic force rotating member 400 is disposed in the installation housing 300;

the driving shaft 510, the middle part of which is disposed in the first pipe body 210, and the upper end of the driving shaft 510 is inserted into the mounting housing 300 and connected with the first magnetic rotating member 400, it is understood that the middle part is not limited to the middle position, and it is understood that all or part of the middle area of the two ends is the middle part, and the upper end of the driving shaft 510 can be connected with the first magnetic rotating member 400 by a general transmission connection manner such as a key connection manner, a threaded connection manner, etc.;

the second magnetic rotating member 600 is sleeved on the first pipe body 210 or the mounting shell 300, the second magnetic rotating member 600 is provided with a stirring blade 700, further, the stirring blade 700 can be integrally formed with the shell of the second magnetic rotating member 600, and can also be arranged on the second magnetic rotating member 600 through a general mounting and fixing structure, and the second magnetic rotating member 600 can be positioned on one axial side of the first magnetic rotating member 400, and can also be positioned on one radial side of the first magnetic rotating member 400;

the rotation of the driving shaft 510 can drive the first magnetic rotator 400 to rotate, and the rotation of the first magnetic rotator 400 can drive the second magnetic rotator 600 to rotate.

The bioreactor of the basic embodiment drives the first magnetic rotating member 400 to rotate through the driving shaft 510, and drives the second magnetic rotating member 600 to rotate by utilizing the magnetic action of the first magnetic rotating member 400, and the inner cavities of the driving shaft 510 and the liquid storage container 100 are isolated by the first pipe body 210 matched with the installation shell 300, so that the bacterial contamination risk caused by the traditional mechanical driving and stirring and the influence of the metal parts of the driving part on the inside of the liquid storage container 100 are avoided, the sterility assurance capability of the bioreactor is improved, and the liquid storage container 100 can be conveniently folded and packaged and can conveniently meet the irradiation sterilization requirement.

Through the structural arrangement of the bioreactor of the basic embodiment, the driving shaft 510 can be free from being arranged into a long stirring shaft structure, so that the liquid storage container 100 can be folded and transported, and the installation process can ensure the tightness of the liquid storage container 100 after the components in the liquid storage container 100 such as the first pipe body 210, the installation shell 300, the first magnetic rotating member 400, the second magnetic rotating member 600 and the like are arranged, so that the driving shaft 510 is inserted into the first pipe body 210, and the driving shaft 510 is connected with the first magnetic rotating member 400 in a transmission way.

The stirring blade is driven by a magnetic driving mode, so that 1000L-6000L of biological cell culture can be met, damage to cells is reduced under the condition of low-shear stirring, and meanwhile, the effect of high mixing is achieved under the condition of low-rotation-speed stirring.

The bioreactor is configured in a lower magnetic stirring mode, so that the bioreactor is conveniently applied to bioreactors with various capacities, a magnetic stirring structure is arranged at other positions relative to the liquid level of the culture solution in the liquid storage container from bottom to top, the stirring range, especially the vertical stirring range, can be enlarged by the lower magnetic stirring mode, and the position of the ventilation disc is convenient for installing components such as the first tube body.

Development of the basic embodiment referring to fig. 2-4, the magnetic stirring assembly further comprises:

the second tube 800 is disposed in the middle of the first tube 210, and the driving shaft 510 is disposed in the middle of the second tube 800, which is understood to be not limited to a middle position, but may be understood to be a middle part of all or part of the middle of two ends, and the second tube 800 is disposed to facilitate protection of the driving shaft 510 and also facilitate filling of a gap between the driving shaft 510 and the first tube 210.

2-4, the first tube 210 is a silicone tube, the second tube 800 is a stainless steel tube, the silicone tube is configured to facilitate folding of the liquid storage container 100, the silicone tube is also a preferred material for isolating the liquid storage container 100 from the driving shaft 510, the stainless steel tube is configured to facilitate the soft silicone tube to be straightened as much as possible by installing the stainless steel tube before installing the driving shaft 510, so that the driving shaft 510 is installed and positioned more accurately when the driving shaft 510 is installed, and the first magnetic rotating member 400 can be connected more conveniently.

It will be appreciated that the first tube may be made of other flexible materials that can meet the cell culture requirements, and the second tube may be made of other rigid materials that can meet the cell culture requirements.

2-4, the lower end of the second pipe body 800 is connected with the ventilation disc 110 in a threaded manner, the upper end of the second pipe body 800 is connected with the installation housing 300 in a threaded manner, the second pipe body 800 is fixed in a threaded manner at two ends, so that the installation and positioning of the driving shaft 510 can be better completed, the variability of the silicone tube is considered, the installation space of the driving shaft 510 can be well limited by limiting the inner pipe diameter of the second pipe body 800 to be slightly larger than the shaft diameter of the driving shaft 510, and the driving shaft 510 is convenient to be connected with the first magnetic rotating member 400.

It will be appreciated that the two ends of the second tube may be connected to the vent disc and the mounting housing by a universal fastening means such as a snap fit means.

Development of the basic embodiment referring to fig. 2-4, the magnetic stirring assembly further comprises:

the mounting plate is arranged below the liquid storage container 100, the lower end of the second pipe body 800 is connected with the mounting plate in a threaded manner, and the upper end of the second pipe body 800 is connected with the mounting shell 300 in a threaded manner;

the driving piece is arranged below the liquid storage container 100, the lower end of the driving shaft 510 penetrates through the ventilation disc and the mounting plate and is connected with the driving piece, specifically, the driving piece can drive the driving shaft 510 to rotate, the driving piece can adopt a motor, specifically, a gear motor, the driving piece can be arranged on the outer wall of the ventilation disc in a threaded mode and other general mounting modes, the driving piece is arranged to drive the driving shaft 510 to rotate outside the liquid storage container 100, and accordingly the first magnetic force rotating piece 400 and the second magnetic force rotating piece 600 are driven to rotate, and the driving piece is arranged outside and is convenient to install.

Further development of the basic embodiment, referring to fig. 2-3, the lower end of the driving shaft 510 is provided with a connecting sleeve 520, the output end of the driving member can be connected with the connecting sleeve 520 in a key connection manner, the arrangement of the connecting sleeve 520 is convenient for the transmission connection between the driving shaft 510 and the driving member, the upper end of the connecting sleeve 520 is fixed at the lower end of the driving shaft 510, the lower end of the connecting sleeve 520 is provided with a groove, a key slot can be arranged in the groove, the output shaft of the driving member can be inserted into the groove, and a key on the output shaft is matched with the key slot.

It will be appreciated that the drive member and the drive shaft may be connected by other common drive connections.

Development of the basic embodiment referring to fig. 3, the lower end of the first pipe body 210 is in interference connection with the inner wall of the vent disc 110, so as to realize sealing connection between the lower end of the first pipe body 210 and the inner wall of the vent disc 110.

It is understood that the inner wall sealing connection of first body lower extreme and vent disc, the upper end of first body and the lower extreme sealing connection of intermediate junction pipe, the upper end of intermediate junction pipe and the installation tube portion sealing connection of installation casing lower extreme, first body, intermediate junction pipe, installation casing cooperate and make the inner chamber of stock solution container and the installation space isolation of second body and drive shaft.

Development of the basic embodiment referring to fig. 2-4, the first tube 210 is a silicone tube;

the lower end of the installation housing 300 includes an installation tube portion 310, the installation tube portion 310 is connected to the upper end of the silicone tube, and the second magnetic rotating member 600 is sleeved on the installation tube portion 310. The arrangement of the mounting tube portion 310 facilitates the connection of the silicone tube and also facilitates the arrangement of the second magnetic rotator 600.

Further development of the basic embodiment, referring to fig. 2-4, the magnetic stirring assembly further comprises:

the upper end of the silicone tube is connected to the lower end of the intermediate connecting tube 910, the installation tube portion 310 is connected to the upper end of the intermediate connecting tube 910, and the sealing connection of the silicone tube and the installation tube portion 310 is facilitated by the arrangement of the intermediate connecting tube 910, so that the sealing performance is ensured. The second magnetic rotator 600 is supported by the intermediate connection pipe 910, and the second magnetic rotator 600 may be installed and fastened in a groove formed between the installation pipe part 310 and the intermediate connection pipe 910, and the weight of the second magnetic rotator 600 is supported by the intermediate connection pipe 910 to enable the installation of the second magnetic rotator 600 to be more stable.

2-4, the lower end of the middle connecting pipe 910 is provided with an annular convex buckle 920, the annular convex buckle 920 can be buckled into the upper end of the silicone tube, and the annular convex buckle 920 is arranged into the upper end of the silicone tube in an extrusion mode, so that sealing connection is realized;

the upper end of the intermediate connection pipe 910 is screwed to the mounting pipe portion 310 to achieve a sealed connection. A sealing ring, a sealing coating or a sealing glue layer may be disposed between the upper end of the intermediate connection pipe 910 and the installation pipe 310, so as to enhance the sealing effect.

It is understood that the two ends of the middle connecting pipe can be connected with the silicone pipe and the mounting pipe part by ultrasonic welding, adding a sealing ring and other general sealing connection modes.

It will be appreciated that the radially outer wall of the mounting tube portion may be provided with an annular recess for mounting the second magnetic rotator, or the second magnetic rotator may be mounted by means of a stepped surface on the mounting tube portion cooperating with a space between the upper ends of the intermediate connection tubes.

Development of the basic embodiment referring to fig. 2-4, the first magnetic rotator 400 includes:

the first rotating shell is connected with the driving shaft 510, and a threaded hole or a key slot can be arranged at the axial center of the first rotating shell to be matched with the upper end of the accommodating driving shaft 510;

the first magnetic piece is arranged in the first rotating shell, the inner cavity of the first rotating shell can be annular, and the shape of the first magnetic piece is annular correspondingly. The first rotating housing is capable of protecting the first magnetic member.

It will be appreciated that the first rotary housing may be provided as a two-part housing cap connection facilitating the provision of the first magnetic member internally.

It will be appreciated that the first magnetic rotator may also be formed directly from a magnetic member.

Further development of the basic embodiment, referring to fig. 2-4, the lower end of the first rotating housing is connected to the inner wall of the lower end of the mounting housing 300 by means of bearings;

the upper end of the first rotating housing is coupled to the inner wall of the upper end of the mounting housing 300 via a bearing. The bearing type connection enables the first rotating housing to rotate more stably relative to the mounting housing 300, and the two sets of bearings cooperate to enable the first rotating housing not to move in the axial direction during rotation.

It will be appreciated that the outer wall of the first rotating housing and the inner wall of the mounting housing may be provided with stepped surfaces or grooves for bearing mounting.

2-4, the second magnetic rotator 600 includes:

the second rotating shell is sleeved on the mounting shell 300;

the second magnetic part is arranged in the second rotating shell, the inner cavity of the second rotating shell can be annular, the shape of the second magnetic part is annular, the radial section of the second magnetic part can be U-shaped, and the inner wall of the lower end of the second rotating shell can be provided with an annular bulge which can be arranged in the second magnetic part. The second rotating housing is capable of protecting the second magnetic member.

It will be appreciated that the second rotary housing may be provided as a two part housing cap connection facilitating the provision of the second magnetic member internally.

It will be appreciated that the second magnetic rotator may also be formed directly from a magnetic member.

It will be appreciated that the magnetic element may be a general purpose magnetic element, and in particular may be a magnet.

Further development of the basic embodiment, referring to fig. 2-4, the second rotating housing is bearing-wise connected to the mounting housing 300. The bearing-wise connection enables the second rotating housing to spin more stably relative to the mounting housing 300.

It will be appreciated that the outer wall of the second rotating housing and the inner wall of the mounting housing may be provided with stepped surfaces or grooves for the mounting of the mating bearings.

2-4, the lower end of the driving shaft 510 extends below the liquid storage container 100, and the lower end of the driving shaft 510 is connected to the outer wall of the vent disc 110 in a bearing manner, so that the driving shaft 510 can rotate more stably relative to the liquid storage container 100. A boss may be disposed on a portion of an outer wall of the vent disc 110 through which the driving shaft 510 is received, a lower end of the driving shaft 510 passes through the boss, an annular groove may be disposed at a lower end of the boss, a bearing may be disposed in the annular groove, and both ends of the bearing are respectively connected with the driving shaft 510 and an inner sidewall of the annular groove.

The expansion of basic embodiment, the stock solution container is the stock solution bag of deformable, and the stock solution bag can adopt the high separation multilayer that accords with pharmaceutical standard to crowd the membrane heat seal to form, and the folding transportation of being convenient for is also convenient for install, is convenient for regard as disposable bioreactor, has saved the clearance work after once cultivateing the completion, and the mounted position is nimble, and the cost is lower.

Development of the basic embodiment, the bioreactor further comprises:

the liquid inlet pipe group is connected with the lower end of the liquid storage container;

the liquid drain pipe group is connected with the upper end of the liquid storage container;

the air inlet pipe group is connected with the lower end and/or the upper end of the liquid storage container;

the exhaust pipe group is connected with the lower end and/or the upper end of the liquid storage container;

the sampling tube group is connected with the upper end of the liquid storage container;

the bubble generating device is arranged in the liquid storage container and is connected with the air charging port on the liquid storage container.

The liquid inlet pipe group can comprise a feeding pipe group, one end of the feeding pipe group is connected with a feeding port of the liquid storage container, and the other end of the feeding pipe group is connected with an external feeding device; the liquid inlet pipe group can also comprise a material supplementing pipe group, one end of the material supplementing pipe group is connected with a material feeding port of the liquid storage container, and the other end of the material supplementing pipe group is connected with an external material supplementing device. The liquid inlet pipe group is used for inputting materials into the liquid storage container according to the requirements.

One end of the liquid drain pipe group is connected with a liquid drain port of the liquid storage container, and the other end is connected with an external harvesting device. The drain pipe group can be used for draining waste liquid and also can be used for harvesting culture liquid.

The air inlet pipe group and the air exhaust pipe group form an air exhaust pipe group which is used for supplying air or exhausting air into the liquid storage container according to requirements.

One end of the sampling tube group is connected with a sampling port of the liquid storage container, and the other end is connected with an external sampling device. The sampling tube set is used for sampling in the liquid storage container.

The bubble generating device is used for generating bubbles in the liquid storage container.

A sterilizing and filtering device can be arranged in the pipeline of the ventilation pipe group to clean the gas passing through the liquid storage container.

The liquid inlet pipe group, the liquid outlet pipe group, the ventilation pipe group, the sampling pipe group and the like are matched, and an environment for culturing requirements is provided in the cell culturing process.

It is noted that terms like "one embodiment," "some embodiments," "base embodiments," "extended embodiments," and the like may be used throughout this specification to describe several embodiments of the utility model, as a particular feature, structure, material, or characteristic of the several embodiments may be combined without departing from the principles and spirit of the present utility model.

While there has been shown and described what is considered to be certain embodiments of the present utility model, it is to be understood that the utility model is not limited to the above-described embodiments, but is to be accorded the widest scope consistent with the principles and novel features of the present utility model.

Claims (10)

1. The lower magnetic stirring type bioreactor is characterized by comprising the following components:

a liquid storage container (100), the lower end of which is provided with a ventilation disc (110);

the first pipe body (210) is arranged in the liquid storage container (100), and the lower end of the first pipe body (210) is connected with the ventilation disc (110);

a mounting housing (300) connected to the upper end of the first pipe body (210);

a first magnetic rotator 400 provided in the installation case 300;

the middle part of the driving shaft (510) is arranged in the first pipe body (210), and the upper end of the driving shaft (510) is inserted into the mounting shell (300) and is connected with the first magnetic force rotating piece (400);

the second magnetic force rotating piece (600) is sleeved on the installation shell (300), and the stirring blade (700) is arranged on the second magnetic force rotating piece (600);

the driving shaft (510) can rotate to drive the first magnetic rotating piece (400) to rotate, and the first magnetic rotating piece (400) can rotate to drive the second magnetic rotating piece (600) to rotate.

2. The lower magnetic stirring bioreactor of claim 1, further comprising:

the middle part of the second pipe body (800) is arranged in the first pipe body (210), and the middle part of the driving shaft (510) is arranged in the second pipe body (800);

the first pipe body (210) is a silica gel pipe, and the second pipe body (800) is a stainless steel pipe.

3. The lower magnetic stirring bioreactor of claim 2, further comprising:

the mounting plate is arranged below the liquid storage container (100), the lower end of the second pipe body (800) is connected with the mounting plate in a threaded mode, and the upper end of the second pipe body (800) is connected with the mounting shell (300) in a threaded mode;

the driving piece is positioned below the liquid storage container (100), the lower end of the driving shaft (510) penetrates through the mounting plate and is connected with the driving piece, and the driving piece can drive the driving shaft (510) to rotate;

the lower end of the driving shaft (510) is provided with a connecting sleeve (520), and the output end of the driving piece is connected with the connecting sleeve (520) in a key connection mode.

4. The lower magnetic stirring bioreactor of claim 1, wherein the lower end of the first tube (210) is in interference connection with the vent disc (110).

5. The lower magnetic stirring bioreactor of claim 1, wherein the first tube (210) is a silicone tube;

the lower end of the installation shell (300) comprises an installation pipe portion (310), the installation pipe portion (310) is connected with the upper end of the silicone tube, and the second magnetic force rotating piece (600) is sleeved on the installation pipe portion (310).

6. The lower magnetic stirring bioreactor of claim 5, further comprising:

the middle connecting pipe (910), the lower extreme is connected the upper end of silicone tube, the upper end of middle connecting pipe (910) is connected installation pipe portion (310), second magnetic force rotates piece (600) by middle connecting pipe (910) support.

7. The lower magnetic stirring bioreactor as set forth in claim 6, wherein the lower end of the middle connecting tube (910) is provided with an annular protruding buckle (920), and the annular protruding buckle (920) can be buckled into the upper end of the silicone tube;

the upper end of the middle connecting pipe (910) is connected with the mounting pipe part (310) in a threaded mode, and a sealing ring is arranged between the upper end of the middle connecting pipe (910) and the mounting pipe part (310).

8. The lower magnetic stirring bioreactor of claim 1, wherein the first magnetic rotator (400) comprises:

a first rotary housing connected to the drive shaft (510);

the first magnetic piece is arranged in the first rotating shell;

the lower end of the first rotating shell is connected with the inner wall of the lower end of the mounting shell (300) in a bearing mode;

the upper end of the first rotating housing is connected with the inner wall of the upper end of the mounting housing (300) in a bearing mode.

9. The lower magnetic stirring bioreactor of claim 1, wherein the second magnetic rotator (600) comprises:

the second rotating shell is sleeved on the mounting shell (300);

the second magnetic piece is arranged in the second rotating shell;

the second rotating housing is connected to the mounting housing (300) via bearings.

10. The lower magnetic stirring bioreactor of claim 1, wherein the lower end of the drive shaft (510) extends below the aeration disc (110), and the lower end of the drive shaft (510) is connected to the outer wall of the aeration disc (110) in a bearing manner.