CN215593062U

CN215593062U - Be applied to bioreactor's exhaust apparatus and biological reaction equipment

Info

Publication number: CN215593062U
Application number: CN202122021942.6U
Authority: CN
Inventors: 刘永连; 郭林; 简伟; 肖海峰
Original assignee: Beijing Minhai Biotechnology Co ltd
Current assignee: Beijing Minhai Biotechnology Co ltd
Priority date: 2021-08-25
Filing date: 2021-08-25
Publication date: 2022-01-21
Anticipated expiration: 2031-08-25

Abstract

The utility model provides an exhaust device applied to a bioreactor and a bioreactor, which comprises a bottle body, a conduit assembly and an air filter, wherein the bottle body is provided with a bottle cap, and the bottle cap is provided with a first interface and a second interface which are communicated with the interior of the bottle body. The duct assembly is respectively communicated with the first interface and the air outlet of the bioreactor, and the air filter is communicated with the corresponding second interface. According to the exhaust device provided by the utility model, the air bubbles discharged from the bioreactor are introduced into the bottle body through the pipe assembly for defoaming and liquefying, and the redundant gas in the bottle body is discharged through the air filter, so that the internal pressure of the bottle body and the bioreactor is ensured to be stable, and the normal fermentation process in the bioreactor is further ensured.

Description

Be applied to bioreactor's exhaust apparatus and biological reaction equipment

Technical Field

The utility model relates to the field of biological reaction equipment, in particular to an exhaust device applied to a bioreactor and biological reaction equipment.

Background

Among the prior art, bioreactor's exhaust apparatus only connects air cleaner at the gas vent end and discharges jar interior gas as the respirator, then can corresponding take out the bubble when jar interior exhaust gas, and the bubble is unable to pass through air cleaner, and blocks up air cleaner part, then can thoroughly block up air cleaner when more bubble, will lead to bioreactor jar interior gas unable discharge like this. This presents some problems: the gas in the tank can not be discharged all the time, so that the pressure in the tank is increased, the culture is damaged, and the whole fermentation process can be stopped when the culture is seriously damaged; the gas in the tank can not be discharged, so that the gas normally required in the fermentation process can not enter the tank, and parameters such as dissolved oxygen, pH value and the like can not be maintained, so that the growth and synthesis of the culture are influenced, and the corresponding product quality is influenced. In addition, the bioreactor used in the prior art partially adopts a tank body which is not resistant to pressure, such as glass, and the safety accident that the tank body is broken can be caused by larger pressure.

SUMMERY OF THE UTILITY MODEL

The utility model provides an exhaust device applied to a bioreactor, which is used for solving the problems that the normal reaction of organisms is influenced and safety accidents are caused because gas cannot be smoothly exhausted in the conventional bioreactor.

The utility model provides an exhaust device applied to a bioreactor, comprising:

the bottle body is provided with a bottle cap, and the bottle cap is provided with a first interface and a second interface which are communicated with the interior of the bottle body;

a conduit assembly which is respectively communicated with the first interface and the air outlet of the bioreactor;

an air filter in communication with the corresponding second interface.

According to the exhaust device applied to the bioreactor, the conduit assembly comprises a first pipe body, a second pipe body and a quick connector, one end of the first pipe body is communicated with the air outlet of the bioreactor, the other end of the first pipe body is communicated with one end of the second pipe body through the quick connector, and the other end of the second pipe body is communicated with the first port.

According to the exhaust device applied to the bioreactor, the quick joint is a CPC quick joint or a luer joint.

According to the exhaust device applied to the bioreactor, provided by the utility model, the first pipe body and the second pipe body are both silica gel hoses.

According to the exhaust device applied to the bioreactor, the conduit assembly further comprises a control valve, the control valve is arranged on the first pipe body, and the control valve is used for controlling the first pipe body to be communicated and closed.

According to the exhaust device applied to the bioreactor, the control valve is a hose clamp.

According to the exhaust device applied to the bioreactor, the air filter is a hydrophobic filter, and the pore diameter of the hydrophobic filter is 0.1-0.4 μm.

According to the exhaust device applied to the bioreactor, the bottle body is made of high-temperature-resistant and high-pressure-resistant materials.

According to the exhaust device applied to the bioreactor, the bottle cap is further provided with a pressure release valve communicated with the interior of the bottle body.

The utility model also provides a bioreactor, which comprises a bioreactor and an exhaust device applied to the bioreactor.

According to the exhaust device provided by the utility model, the air bubbles discharged from the bioreactor are introduced into the bottle body through the pipe assembly for defoaming and liquefying, and the redundant gas in the bottle body is discharged through the air filter, so that the internal pressure of the bottle body and the bioreactor is ensured to be stable, and the normal fermentation process in the bioreactor is further ensured.

Drawings

In order to more clearly illustrate the technical solutions of the present invention or the prior art, the drawings needed for the description of the embodiments or the prior art will be briefly described below, and it is obvious that the drawings in the following description are some embodiments of the present invention, and those skilled in the art can also obtain other drawings according to the drawings without creative efforts.

FIG. 1 is a schematic structural diagram of an exhaust apparatus applied to a bioreactor according to an embodiment of the present invention.

Reference numerals:

10. a bottle body; 11. a bottle cap; 12. an air filter; 13. a first pipe body; 14. a second tube body; 15. a quick coupling; 16. a hose clamp.

Detailed Description

The embodiments of the present invention will be described in further detail with reference to the drawings and examples. The following examples are intended to illustrate the utility model but are not intended to limit the scope of the utility model.

In the description of the embodiments of the present invention, it should be noted that the terms "center", "longitudinal", "lateral", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", and the like indicate orientations or positional relationships based on those shown in the drawings, and are only for convenience in describing the embodiments of the present invention and simplifying the description, but do not indicate or imply that the referred devices or elements must have a specific orientation, be constructed in a specific orientation, and be operated, and thus, should not be construed as limiting the embodiments of the present invention. Furthermore, the terms "first," "second," and "third" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.

In the description of the embodiments of the present invention, it should be noted that, unless explicitly stated or limited otherwise, the terms "connected" and "connected" are to be interpreted broadly, and may be, for example, fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; may be directly connected or indirectly connected through an intermediate. Specific meanings of the above terms in the embodiments of the present invention can be understood in specific cases by those of ordinary skill in the art.

In embodiments of the utility model, unless expressly stated or limited otherwise, the first feature "on" or "under" the second feature may be directly contacting the first and second features or indirectly contacting the first and second features through intervening media. Also, a first feature "on," "over," and "above" a second feature may be directly or diagonally above the second feature, or may simply indicate that the first feature is at a higher level than the second feature. A first feature being "under," "below," and "beneath" a second feature may be directly under or obliquely under the first feature, or may simply mean that the first feature is at a lesser elevation than the second feature.

In the description herein, references to the description of the term "one embodiment," "some embodiments," "an example," "a specific example," or "some examples," etc., mean that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of an embodiment of the utility model. In this specification, the schematic representations of the terms used above are not necessarily intended to refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples. Furthermore, various embodiments or examples and features of different embodiments or examples described in this specification can be combined and combined by one skilled in the art without contradiction.

Fig. 1 illustrates a schematic structural view of a degassing apparatus for a bioreactor according to an embodiment of the present invention, as shown in fig. 1, the degassing apparatus for a bioreactor includes a bottle 10, a conduit assembly and an air filter 12, the bottle 10 is used for collecting bubbles discharged from the bioreactor and liquid liquefied from the bubbles, the bottle 10 is provided with a bottle cap 11, the bottle cap 11 is hermetically connected to the bottle 10, the bottle cap 11 is located at the top of the bottle 10, and the bottle cap 11 is provided with a first port and two second ports communicating with the interior of the bottle 10. The duct assembly is in communication with the first port and the air outlet of the bioreactor, respectively, and the air filter 12 is in communication with the corresponding second port.

The exhaust device provided by the utility model guides the bubbles discharged from the bioreactor into the bottle body 10 through the duct assembly for defoaming and liquefying, and exhausts the redundant gas in the bottle body 10 through the air filter 12, thereby ensuring the stable internal pressure of the bottle body 10 and the bioreactor and further ensuring the normal fermentation process in the bioreactor.

According to an embodiment of the present invention, the catheter assembly includes a first tube 13, a second tube 14, and a quick coupling 15, one end of the first tube 13 is communicated with the air outlet of the bioreactor, the other end of the first tube 13 is communicated with one end of the second tube 14 through the quick coupling 15, and the other end of the second tube 14 is communicated with the first port. Set up quick-operation joint 15 and can conveniently change bottle 10, when the bubble and the liquid collection of bottle 10 inside are more, can continue to collect the bubble that bioreactor cell culture in-process produced through empty bottle 10 through quick-operation joint 15 aseptic change for empty bottle 10 in superclean bench, avoid the inside bubble of bottle 10 and liquid to collect the problem that can't continue to use after full, improve exhaust efficiency.

According to an embodiment of the present invention, the quick coupling 15 is a CPC quick coupling or a luer coupling.

According to the embodiment of the utility model, the first tube 13 and the second tube 14 are both silica gel hoses, and the silica gel hoses are made of high-temperature and high-pressure resistant materials, so that sterilization treatment is easy to perform.

According to an embodiment of the present invention, the catheter assembly further includes a control valve disposed on the first tube 13, the control valve is used for controlling the first tube 13 to open and close, and the control valve is a hose clamp 16. The control valve can be used for intercepting gas exhausted from the bioreactor, controlling the internal pressure of the bioreactor and facilitating the process operations of sampling, liquid changing and the like of the bioreactor cell culture process.

According to an embodiment of the utility model, the air filter 12 is a hydrophobic filter having a pore size of 0.1 μm to 0.4 μm, preferably 0.2 μm. The air filters 12 are provided in two, and the surplus gas inside the bottle body 10 is discharged through the two air filters 12. Compare traditional single filter core exhaust, set up two air cleaner 12, improved the exhaust rate of system, also effectively reduced air cleaner 12 simultaneously and drenched the risk of blockking up by the bubble.

It should be noted here that the number of the air filters 12 is not limited to two, and three or more air filters 12 may be provided, specifically, according to the exhaust gas amount of the bioreactor. The second ports correspond to the air filters 12 one by one, and three or more second ports may be provided.

According to the embodiment of the utility model, the bottle body 10 is made of high-temperature and high-pressure resistant glass, and the high-temperature and high-pressure resistant glass is used, so that the pressure resistance of the bottle body 10 can be effectively improved, and the risk that the bottle body 10 bursts after the air filter 12 is wetted and blocked by bubbles is reduced.

According to the embodiment of the utility model, the bottle cap 11 is further provided with a pressure relief valve communicated with the interior of the bottle body 10, and the pressure relief value of the pressure relief valve is smaller than the rated pressure value of the bottle body 10. After the air filter 12 is wetted by the bubbles and is blocked up, the internal pressure of the bottle body 10 is increased, and when the internal pressure of the bottle body 10 reaches a pressure relief value, the pressure relief valve is automatically opened to relieve the pressure, so that the bottle body 10 is prevented from bursting due to overlarge pressure.

According to an embodiment of the present invention, as shown in fig. 1, an air discharging apparatus applied to a bioreactor includes a bottle 10, a duct assembly and two air filters 12, the bottle 10 is used for collecting bubbles discharged from the bioreactor and liquid liquefied from the bubbles, the bottle 10 is made of glass resistant to high temperature and high pressure, the bottle 10 is provided with a cap 11, the cap 11 is hermetically connected to the bottle 10, the cap 11 is located at the top of the bottle 10, and the cap 11 is provided with a first port and two second ports communicating with the inside of the bottle 10.

The conduit subassembly includes first body 13, second body 14, quick-operation joint 15 and control valve, and the one end of first body 13 and bioreactor's gas outlet intercommunication, the other end of first body 13 pass through quick-operation joint 15 and the one end intercommunication of second body 14, the other end and the first interface intercommunication of second body 14. Quick-operation joint 15 is CPC quick-operation joint or luer and connects, and first body 13 and second body 14 are the silica gel hose, and the silica gel hose is high temperature resistant high pressure material. The control valve is disposed on the first tube 13, and the control valve is used to control the first tube 13 to open and close, and the control valve is a hose clamp 16.

Two air filters 12 are arranged, the two air filters 12 are in one-to-one correspondence communication with the two second ports, and the air filters 12 are hydrophobic filters with the pore diameter of 0.2 μm.

According to the exhaust device provided by the utility model, the two air filters 12 are arranged, so that the exhaust efficiency can be improved, and the failure of the fermentation process caused by the blockage of the air filters 12 can be effectively reduced. The exhaust device applied to the bioreactor can realize high-efficiency exhaust of the bioreactor, and can also realize quick replacement of the bottle body 10 in a sterile environment, thereby realizing continuous collection of bubbles exhausted in a cell culture process of the bioreactor. The bioreactor exhaust device of the utility model basically does not change the original process exhaust mode, so that the culture process is not influenced, the exhaust efficiency of the bioreactor is improved, and the risk caused by unsmooth exhaust in the cell culture process of the bioreactor is avoided.

Bioreactor cell culture process need be to the bioreactor in let in gas maintains the stability of dissolved oxygen and the pH valve isoparametric of fermentation process, along with gaseous entering bioreactor in, the culture solution will produce the bubble, when the bubble is more, bioreactor then can take the bubble out at exhaust gas's in-process, the bubble of taking out (the arrow in figure 1 shows the bubble) carries out the defoaming liquefaction through first body 13 and second body 14 entering bottle 10 in proper order, unnecessary gas then discharges through two air cleaner 12, thereby guarantee system pressure stability.

The utility model also provides a bioreactor, which comprises a bioreactor and an exhaust device applied to the bioreactor in any one of the embodiments.

Finally, it should be noted that: the above examples are only intended to illustrate the technical solution of the present invention, but not to limit it; although the present invention has been described in detail with reference to the foregoing embodiments, it will be understood by those of ordinary skill in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some technical features may be equivalently replaced; and such modifications or substitutions do not depart from the spirit and scope of the corresponding technical solutions of the embodiments of the present invention.

Claims

1. An exhaust apparatus for use in a bioreactor, comprising:

an air filter in communication with the corresponding second interface.

2. The exhaust apparatus applied to a bioreactor according to claim 1, wherein the conduit assembly comprises a first pipe, a second pipe and a quick coupling, one end of the first pipe is communicated with the gas outlet of the bioreactor, the other end of the first pipe is communicated with one end of the second pipe through the quick coupling, and the other end of the second pipe is communicated with the first port.

3. The exhaust apparatus applied to the bioreactor according to claim 2, wherein the quick coupling is a CPC quick coupling or a luer coupling.

4. The exhaust apparatus applied to the bioreactor according to claim 2 or 3, wherein the first pipe and the second pipe are both silica gel hoses.

5. The exhaust apparatus applied to the bioreactor as claimed in claim 2 or 3, wherein the conduit assembly further comprises a control valve disposed on the first pipe, and the control valve is used for controlling the opening and closing of the first pipe.

6. The exhaust apparatus applied to the bioreactor according to claim 5, wherein the control valve is a hose clamp.

7. The exhaust apparatus applied to the bioreactor according to any one of claims 1 to 3, wherein the air filter is a hydrophobic filter having a pore size of 0.1 μm to 0.4 μm.

8. The exhaust apparatus for bioreactor according to any one of claims 1 to 3, wherein the bottle body is made of a material resistant to high temperature and high pressure.

9. The exhaust device applied to the bioreactor according to any one of claims 1 to 3, wherein a pressure relief valve communicated with the interior of the bottle body is further provided on the bottle cap.

10. A bioreactor comprising a bioreactor, characterized in that it further comprises an exhaust device as claimed in any one of claims 1 to 9 applied to the bioreactor.