CN215530661U - Plant tissue culture bottle - Google Patents

Plant tissue culture bottle Download PDF

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Publication number
CN215530661U
CN215530661U CN202120967990.1U CN202120967990U CN215530661U CN 215530661 U CN215530661 U CN 215530661U CN 202120967990 U CN202120967990 U CN 202120967990U CN 215530661 U CN215530661 U CN 215530661U
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China
Prior art keywords
bottle
bottle body
base
plant tissue
tissue culture
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CN202120967990.1U
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Chinese (zh)
Inventor
刘小娥
苏世平
武利玉
卢嘉
马瑞
马敏
单立山
张晓玮
马彦军
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Gansu Agricultural University
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Gansu Agricultural University
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Abstract

The utility model discloses a plant tissue culture bottle, which comprises a bottle body and a bottle cap detachably connected with the bottle body, wherein an installation component is arranged in the bottle body, a ventilating component and an auxiliary component are arranged on the bottle cap, the installation component is used for replacing agar, a liquid culture medium is adopted for plant tissue culture, the plant tissue culture bottle comprises a base, a plurality of installation holes are formed in the base, and four bottom rods for preventing the base from being in direct contact with the bottom of the bottle body are arranged at the bottom of the base along the central annular array of the base; ventilative subassembly is used for making things convenient for the circulation of air, including setting up the bleeder vent in bottle lid central authorities, fixedly connected with prevents that the foreign matter from getting into the first net that separates in the body inner chamber on the bleeder vent, uses through the cooperation of installation component, ventilative subassembly and auxiliary assembly during the use, and convenience of customers carries out biological assay, is favorable to improving user's use and feels and the stability when experimenting, and does not adopt the non-woven fabrics to carry out ventilative filtration, has prolonged effective life, and convenience of customers uses repeatedly many times.

Description

Plant tissue culture bottle
Technical Field
The utility model relates to the technical field of biological experimental equipment, in particular to a plant tissue culture bottle.
Background
The cultivation of plant tissues is generally carried out in culture flasks, and with the development of biotechnology, tissue culture technology has become widely widespread and is widely used as a culture flask for use in tissue culture technology.
The culture bottle that uses among the prior art comprises bottle and bottle lid two parts usually, has a little bleeder vent of a dustproof material preparation on the bottle lid for breathe freely when cultivateing the plant, supply plant tissue growth needs, this bleeder vent mainly is used to have the good non-woven fabrics preparation of gas permeability at present, but along with the increase of number of times of use, the non-woven fabrics is fragile, in case damaged, this bottle lid just can't continue to use. Even if among the prior art still use the culture bottle lid, also be two kinds, one kind is sealed lid, a wet ventilative lid, sealed lid tissue bottle is airtight, influence plant tissue development, and the humidity reduces too fast in the tissue bottle of ventilative lid, also can influence tissue development, in addition at the in-process that tissue developed, water or nutrient solution need be supplemented to in the bottle usually, open the bottle lid and can lead to the tissue in the culture bottle to suffer little biological contamination usually in prior art, influence plant tissue development, and simultaneously, need add agar and fix the plant material in the conventional group banks up with earth, the cost is higher.
In view of the above problems, a plant tissue culture flask is now designed.
SUMMERY OF THE UTILITY MODEL
The present invention is directed to a plant tissue culture flask, which solves the above problems of the prior art.
In order to achieve the purpose, the utility model provides the following technical scheme:
a plant tissue culture bottle comprises a bottle body and a bottle cap detachably connected with the bottle body, wherein an installation assembly is arranged in the bottle body, a ventilating assembly and an auxiliary assembly are arranged on the bottle cap, the installation assembly is used for replacing agar to perform plant tissue culture by adopting a liquid culture medium and comprises a base, a plurality of installation holes are formed in the base, and four bottom rods for preventing the base from being in direct contact with the bottom of the bottle body are arranged at the bottom of the base along the central annular array of the base;
the air-permeable component is used for facilitating air circulation and comprises an air vent arranged in the center of the bottle cap, a first separation net for preventing foreign matters from entering the inner cavity of the bottle body is fixedly connected to the air vent, and a connecting pipe connected with the air vent is arranged on the inner wall of the bottle body;
the auxiliary assembly is used for facilitating a user to add liquid substances or measure the temperature and humidity in the bottle body and comprises auxiliary holes formed in the bottle cover on one side of the air holes.
As a further scheme of the utility model: fixedly connected with prevents that the dust from getting into the toper elastic block in the body from the auxiliary hole on the auxiliary hole, toper elastic block is formed by the equipment of the semicircle toper silica gel piece that two symmetries set up.
As a still further scheme of the utility model: can dismantle in the connecting tube inner chamber and be connected with the cotton filler that carries out the filtration to dust, bacterium and microorganism, one side that the connecting tube is close to the body can be dismantled through the screw and be connected with the tube cap, be equipped with the second that makes connecting tube and body inner chamber communicate on the tube cap and separate the net.
As a still further scheme of the utility model: the bottle cap is characterized in that the inner wall of the bottle cap is provided with an external thread detachably connected with the bottle body, and the head part of the outer wall of the bottle body is provided with an internal thread matched with the external thread for use.
As a still further scheme of the utility model: the utility model discloses a pedestal, including base, base top, limiting ring, a plurality of dead levers fixed connection between limiting ring and the sill bar, the base top is equipped with convenience of customers and carries out the spacing ring that removes to the base, through a plurality of dead lever fixed connection between spacing ring and the sill bar.
As a still further scheme of the utility model: and the auxiliary hole is in threaded connection with a dustproof cover for preventing dust from entering the auxiliary hole.
As a still further scheme of the utility model: the bottle body is formed by transparent glass material preparation, be equipped with the scale sign that is convenient for the user to observe the liquid level volume on the body outer wall, be equipped with anti-skidding line on the bottle lid outer wall, the bottle lid inner wall is equipped with the sealing washer.
Compared with the prior art, the utility model has the beneficial effects that:
when the biological experiment device is used, the installation component, the ventilation component and the auxiliary component are matched for use, so that a user can conveniently carry out a biological experiment, the use feeling of the user and the stability during the experiment are improved, a non-woven fabric is not adopted for ventilation and filtration, the effective service life is prolonged, and the repeated use by the user is facilitated.
2, when the bottle is used, the plant tissue is fixed through the base, so that the stability of the experiment is improved, the bottom rod at the bottom of the base can prevent the base from being in direct contact with the bottom of the bottle body, and the plant component can grow conveniently.
3, in the use process, the connecting pipe, the second separation net, the pipe cover, the air holes and the first separation net are matched for use, so that ventilation is facilitated, plants can grow conveniently, through the matching use of the auxiliary holes and the conical elastic blocks, the sealing performance is good, dust and the like are prevented from entering the inner cavity of the bottle body, liquid substances can be conveniently added into the bottle body through the needle of the injector and the like by a user during an experiment, monitoring probes for temperature, humidity and the like can be inserted into the auxiliary holes, and the study on the temperature and the humidity in the bottle body by the user is facilitated.
Drawings
FIG. 1 is a schematic structural diagram of the present invention.
Fig. 2 is a schematic structural view of the bottle body of the present invention.
Fig. 3 is a schematic structural view of the bottle cap of the present invention.
Fig. 4 is a schematic view of the structure of the mounting assembly of the present invention.
FIG. 5 is a schematic view of the structure of the auxiliary hole of the present invention.
Wherein: 11, a bottle body; 12, a bottle cap; 13, air holes; 14, a first screen; 15, auxiliary holes; 16, scale mark; 17, internal threads; 18, a dust cover; 19, external threads; 20, anti-skid lines; 21, a second screen; 22, a tube cover; 23, a connecting pipe; 24, a tapered resilient block; 25, a limiting ring; 26, fixing the rod; 27, a bottom bar; 28, a base; 29, mounting holes.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Referring to fig. 1-5, in an embodiment of the present invention, a plant tissue culture bottle includes a bottle body 11 and a bottle cap 12 detachably connected to the bottle body 11, wherein a mounting assembly is disposed in the bottle body 11, a gas permeable assembly and an auxiliary assembly are disposed on the bottle cap 12, the mounting assembly is used for performing plant tissue culture using a liquid culture medium instead of agar, and includes a base 28, a plurality of mounting holes 29 are disposed on the base 28, and four bottom rods 27 are disposed at the bottom of the base 28 along a central annular array of the base 28 to prevent the base 28 from directly contacting the bottom of the bottle body 11; through the cooperation use of installation component, ventilative subassembly and auxiliary assembly during the use, convenience of customers carries out biological assay, is favorable to improving user's use and feels and the stability when experimenting, and does not adopt the non-woven fabrics to breathe freely and filter, has prolonged effective life, and convenience of customers uses repeatedly many times.
The ventilation component is used for facilitating air circulation and comprises a ventilation hole 13 arranged in the center of the bottle cap 12, a first separation net 14 for preventing foreign matters from entering the inner cavity of the bottle body 11 is fixedly connected to the ventilation hole 13, and a connecting pipe 23 connected with the ventilation hole 13 is arranged on the inner wall of the bottle body 11;
the auxiliary assembly is used for facilitating a user to add liquid substances or measure the temperature and humidity in the bottle body 11 and comprises an auxiliary hole 15 formed in the bottle cap 12 on one side of the air hole 13.
Fixedly connected with prevents that the dust from getting into the toper elastic block 24 in the body 11 from auxiliary hole 15 on auxiliary hole 15, toper elastic block 24 is formed by the equipment of the semicircle toper silica gel piece that two symmetries set up, uses through auxiliary hole 15 and the cooperation of toper elastic block 24, and not only the leakproofness is better, avoids dust etc. to get into in the 11 inner chambers of body, the user adds the liquid substance to body 11 through syringe needle etc. when still making things convenient for the experiment, also can insert monitoring probe such as temperature, humidity at auxiliary hole 15, help the user to the research of humiture in the body 11.
Can dismantle in the connecting pipe 23 inner chamber and be connected with and carry out filterable cotton filler to dust, bacterium and microorganism, one side that connecting pipe 23 is close to body 11 can be dismantled through the screw and be connected with tube cap 22, be equipped with the second that makes connecting pipe 23 and body 11 inner chamber intercommunication on the tube cap 22 and separate net 21, in the use, separate net 21, tube cap 22, bleeder vent 13 and the first cooperation that separates net 14 through connecting pipe 23, second and use, conveniently breathe freely, the plant of being convenient for grows.
The inner wall of the bottle cap 12 is provided with an external thread 19 detachably connected with the bottle body 11, and the head of the outer wall of the bottle body 11 is provided with an internal thread 17 matched with the external thread 19 for use.
28 top of base is equipped with convenience of customers and carries out the spacing ring 25 that removes to base 28, through a plurality of dead lever 26 fixed connection between spacing ring 25 and the sill bar 27, auxiliary hole 15 department threaded connection has the shield 18 that prevents the dust entering auxiliary hole 15, during the use, fixes the plant tissue through base 28, improves the stability of experiment, and the sill bar 27 of base 28 bottom can avoid base 28 and body 11 bottom direct contact, and the plant component of being convenient for grows.
The bottle body 11 is made by transparent glass material, be equipped with the scale sign 16 that is convenient for the user to observe the liquid level volume on the body 11 outer wall, be equipped with anti-skidding line 20 on the bottle lid 12 outer wall, the bottle lid 12 inner wall is equipped with the sealing washer, and convenience of customers rotates bottle lid 12.
The working principle of the utility model is as follows: during the use, plant tissue is fixed through base 28, the stability of experiment is improved, the sill bar 27 of base 28 bottom, can avoid base 28 and 11 bottom direct contacts of body, the plant component of being convenient for grows, connecting pipe 23 on bottle lid 12, the second separates net 21, tube cap 22, bleeder vent 13 and the first cooperation that separates net 14 are used, conveniently ventilate, the plant of being convenient for grows, auxiliary hole 15 and conical elastic block 24's cooperation is used on the bottle lid 12, can avoid dust etc. to get into in the 11 inner chambers of body, the user adds to body 11 through syringe needle etc. during still convenient experiment, also can insert monitoring probe such as temperature, humidity in auxiliary hole 15, help the user to the research of humiture in the body 11.
It will be evident to those skilled in the art that the utility model is not limited to the details of the foregoing illustrative embodiments, and that the present invention may be embodied in other specific forms without departing from the spirit or essential attributes thereof.

Claims (7)

1. A plant tissue culture bottle comprises a bottle body (11) and a bottle cap (12) detachably connected with the bottle body (11), wherein a mounting assembly is arranged in the bottle body (11), and a ventilating assembly and an auxiliary assembly are arranged on the bottle cap (12), and the plant tissue culture bottle is characterized in that the mounting assembly is used for replacing agar to perform plant tissue culture by adopting a liquid culture medium and comprises a base (28), a plurality of mounting holes (29) are formed in the base (28), and four bottom rods (27) for preventing the base (28) from being in direct contact with the bottom of the bottle body (11) are arranged at the bottom of the base (28) along the central annular array of the base (28);
the ventilating component is used for facilitating air circulation and comprises a ventilating hole (13) arranged in the center of the bottle cap (12), a first separation net (14) for preventing foreign matters from entering the inner cavity of the bottle body (11) is fixedly connected to the ventilating hole (13), and a connecting pipe (23) connected with the ventilating hole (13) is arranged on the inner wall of the bottle body (11);
the auxiliary assembly is used for facilitating a user to add liquid substances or measure the temperature and humidity in the bottle body (11) and comprises an auxiliary hole (15) formed in the bottle cover (12) on one side of the air hole (13).
2. The plant tissue culture bottle according to claim 1, wherein a conical elastic block (24) for preventing dust from entering the bottle body (11) from the auxiliary hole (15) is fixedly connected to the auxiliary hole (15), and the conical elastic block (24) is formed by assembling two symmetrically arranged semi-conical silica gel blocks.
3. The plant tissue culture bottle according to claim 1, wherein cotton filler for filtering dust, bacteria and microorganisms is detachably connected to the inner cavity of the connecting pipe (23), a pipe cover (22) is detachably connected to one side of the connecting pipe (23) close to the bottle body (11) through a screw thread, and a second separation net (21) for communicating the connecting pipe (23) with the inner cavity of the bottle body (11) is arranged on the pipe cover (22).
4. The plant tissue culture bottle according to claim 1, wherein the inner wall of the bottle cap (12) is provided with an external thread (19) for detachable connection with the bottle body (11), and the head part of the outer wall of the bottle body (11) is provided with an internal thread (17) matched with the external thread (19).
5. The plant tissue culture bottle as claimed in claim 1, wherein a limiting ring (25) is arranged above the base (28) for facilitating the movement of the base (28) by a user, and the limiting ring (25) and the bottom rod (27) are fixedly connected through a plurality of fixing rods (26).
6. The plant tissue culture flask according to claim 1, wherein a dust cap (18) for preventing dust from entering into the auxiliary hole (15) is screwed to the auxiliary hole (15).
7. The plant tissue culture bottle according to claim 1, wherein the bottle body (11) is made of transparent glass material, the outer wall of the bottle body (11) is provided with a scale mark (16) which is convenient for a user to observe the liquid level and the volume, the outer wall of the bottle cap (12) is provided with anti-skid grains (20), and the inner wall of the bottle cap (12) is provided with a sealing ring.
CN202120967990.1U 2021-05-08 2021-05-08 Plant tissue culture bottle Active CN215530661U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202120967990.1U CN215530661U (en) 2021-05-08 2021-05-08 Plant tissue culture bottle

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202120967990.1U CN215530661U (en) 2021-05-08 2021-05-08 Plant tissue culture bottle

Publications (1)

Publication Number Publication Date
CN215530661U true CN215530661U (en) 2022-01-18

Family

ID=79859202

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202120967990.1U Active CN215530661U (en) 2021-05-08 2021-05-08 Plant tissue culture bottle

Country Status (1)

Country Link
CN (1) CN215530661U (en)

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