CN215365796U

CN215365796U - Human oral epidermal cell culture kit for gene sequencing

Info

Publication number: CN215365796U
Application number: CN202120401572.6U
Authority: CN
Inventors: 许恩顺; 梁宁孝; 王海珍; 何奕辉
Original assignee: Nanjing Diji Biotechnology Co ltd
Current assignee: Nanjing Diji Biotechnology Co ltd
Priority date: 2021-02-23
Filing date: 2021-02-23
Publication date: 2021-12-31
Anticipated expiration: 2031-02-23

Abstract

The utility model relates to the technical field of cell culture kits, and discloses a human oral epidermal cell culture kit for gene sequencing, which comprises a culture box, wherein the culture box is made of transparent materials, three support rods are fixedly connected to the outer side wall of the top, close to the middle part of the culture box, of the culture box in a matrix distribution manner, the three support rods are uniformly and equidistantly, the upper ends of the three support rods are jointly and fixedly connected with a support cylinder with a closed lower end, the support cylinder is arranged in a cylindrical manner, a liquid inlet pipe is fixedly communicated with the middle part of the outer cylinder wall at the bottom of the support cylinder, the lower end of the liquid inlet pipe is communicated with the culture box, a first valve is arranged on the liquid inlet pipe, a circle of threads are fixedly arranged on the annular outer cylinder wall of the support cylinder, a scale mark is fixedly arranged on the vertical outer cylinder wall on one side of the support cylinder, and a nut is in threaded connection with the annular outer cylinder wall of the support cylinder. The utility model is convenient for adding accurate amount of nutrient solution into the culture box.

Description

Human oral epidermal cell culture kit for gene sequencing

Technical Field

The utility model relates to the technical field of cell culture kits, in particular to a human oral epidermal cell culture kit for gene sequencing.

Background

Before human oral cavity epidermal cells carry out gene sequencing, need to get up the cell culture of collecting, and then need use the culture box, carry out the in-process of cultivateing and need regular addition nutrient solution, but the culture solution volume that different cells need is different, and the experience that relies on the staff to accumulate for a long time now adds, and then influences the efficiency of test, for this reason, we propose a human oral cavity epidermal cells culture kit for gene sequencing.

SUMMERY OF THE UTILITY MODEL

The utility model aims to solve the problems of the existing human oral epidermic cell culture kit in the prior art in the using process, and provides a human oral epidermic cell culture kit for gene sequencing.

In order to achieve the purpose, the utility model adopts the following technical scheme:

a human oral cavity epidermal cell culture kit for gene sequencing comprises a culture box, wherein the culture box is made of transparent materials, three support rods are fixedly connected to the outer side wall of the top, close to the middle part of the culture box, of the culture box in a matrix distribution uniform and equidistant manner, a support barrel with a closed lower end is fixedly connected to the upper ends of the three support rods together, the support barrel is arranged in a cylindrical manner, a liquid inlet pipe is fixedly communicated with the middle part of the outer barrel wall at the bottom of the support barrel, the lower end of the liquid inlet pipe is communicated with the culture box, a first valve is installed on the liquid inlet pipe, a circle of thread is fixedly arranged on the annular outer barrel wall of the support barrel, a scale mark is fixedly arranged on the vertical outer barrel wall at one side of the support barrel, a nut is connected to the annular outer barrel wall of the support barrel in a threaded manner, a cylindrical adjusting barrel is fixedly connected to the outer barrel wall of the nut, and a sealing mechanism matched with the adjusting barrel is installed on the annular outer barrel wall, close to the top of the support barrel, the adjusting cylinder is fixedly communicated with a liquid leaking pipe with an L-shaped section, the liquid leaking pipe is arranged right above the supporting cylinder, the outer box wall of one side, close to the bottom, of the culture box is fixedly communicated with a liquid outlet pipe, and a second valve is arranged on the liquid outlet pipe.

Preferably, the sealing mechanism comprises a sealing groove and a sealing gasket, the cross section of the sealing groove is annularly arranged, the sealing groove is formed in the annular outer cylinder wall, close to the top, of the supporting cylinder, the sealing gasket is fixedly connected into the sealing groove, and one end, far away from the bottom of the sealing groove, of the sealing gasket penetrates through a notch of the sealing groove and extends outwards and is in sealing sliding connection with the inner cylinder wall of the adjusting cylinder.

Preferably, the cultivation box is close to fixedly connected with magnetite on the top lateral wall of bracing piece one side, be provided with upper end open-ended collection cup on the top lateral wall of magnetite, and the material of collecting the cup is iron.

Preferably, the rotating rods are symmetrically and fixedly connected to the outer cylinder wall of the adjusting cylinder, which is located right above the liquid leakage pipe and is opposite to the liquid leakage pipe.

Preferably, a base is fixedly connected to the side wall of the bottom of the culture box.

Preferably, a barrel cover is hinged to the barrel wall at the top of the adjusting barrel.

Compared with the prior art, the utility model provides a human oral epidermal cell culture kit for gene sequencing, which has the following beneficial effects:

the human oral epidermic cell culture kit for gene sequencing comprises a culture box, a support cylinder, a liquid inlet pipe, a nut, an adjusting cylinder, a liquid leakage pipe, a collecting cup and a sealing mechanism, wherein when culture liquid is added, the adjusting cylinder is rotated according to the amount to be added, the amount of the culture liquid which can be contained between the adjusting cylinder and the support cylinder can be changed along with the rotation of the adjusting cylinder, according to the position of the cylinder wall at the bottom of the adjusting cylinder corresponding to a scale mark, a worker can accurately judge the amount which can be contained between the adjusting cylinder and the support cylinder, after the adjustment to a proper position, the worker can directly add nutrient liquid between the adjusting cylinder and the support cylinder, meanwhile, the collecting cup is placed under the liquid leakage pipe far away from the pipe orifice of the adjusting cylinder, when too much nutrient liquid is added, the redundant nutrient liquid can drip into the collecting cup through the liquid pipe, and the addition is stopped, then opening the first valve to add the nutrient solution with accurate amount into the culture box; the nutrient solution with accurate amount can be conveniently added into the culture box.

The parts not involved in the device are the same as or can be realized by adopting the prior art, and the utility model is convenient for adding accurate amount of nutrient solution into the culture box.

Drawings

FIG. 1 is a schematic structural diagram of a human oral epidermal cell culture kit for gene sequencing according to the present invention;

FIG. 2 is an enlarged view of portion A of FIG. 1;

fig. 3 is an enlarged view of a portion B in fig. 1.

In the figure: 1 culture box, 2 support cylinders, 3 liquid inlet pipes, 4 nuts, 5 adjusting cylinders, 6 liquid leakage pipes, 7 magnets, 8 collecting cups, 9 sealing mechanisms, 91 sealing grooves, 92 sealing gaskets and 10 rotating rods.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments.

Referring to fig. 1-3, a human oral epidermic cell culture kit for gene sequencing, comprising a culture box 1, wherein the culture box 1 is made of transparent material, three support rods are fixedly connected to the outer side wall of the top of the culture box 1 near the middle part in a matrix distribution manner at equal intervals, the upper ends of the three support rods are fixedly connected with a support cylinder 2 with a closed lower end, the support cylinder 2 is arranged in a cylindrical manner, the middle part of the outer cylinder wall at the bottom of the support cylinder 2 is fixedly communicated with a liquid inlet pipe 3, the lower end of the liquid inlet pipe 3 is communicated with the culture box 1, a first valve is arranged on the liquid inlet pipe 3, a circle of screw thread is fixedly arranged on the annular outer cylinder wall of the support cylinder 2, a scale mark is fixedly arranged on the vertical outer cylinder wall of one side of the support cylinder 2, a nut 4 is connected to the annular outer cylinder wall of the support cylinder 2 in a screw thread manner, a cylindrical adjusting cylinder 5 is fixedly connected to the outer side wall of the nut 4, the annular outer cylinder wall close to the top of the supporting cylinder 2 is provided with a sealing mechanism 9 matched with the adjusting cylinder 5, the annular outer cylinder wall at one side of the adjusting cylinder 5 close to the top is fixedly communicated with a liquid leakage pipe 6 with an L-shaped section, the liquid leakage pipe 6 is arranged right above the supporting cylinder 2, the outer cylinder wall at one side of the culture box 1 close to the bottom is fixedly communicated with a liquid outlet pipe, a second valve is arranged on the liquid outlet pipe, when the culture liquid is added, the adjusting cylinder 5 is rotated according to the amount required to be added, the amount of the culture liquid which can be contained between the adjusting cylinder 5 and the supporting cylinder 2 can be changed along with the rotation of the adjusting cylinder 5, according to the position of the cylinder wall at the bottom of the adjusting cylinder 5 corresponding to the scale mark, a worker can accurately judge the amount which can be contained between the adjusting cylinder 5 and the supporting cylinder 2, after the adjustment to a proper position, the worker can directly add the nutrient solution between the adjusting cylinder 5 and the supporting cylinder 2, meanwhile, the collecting cup 8 is placed under the liquid leakage pipe 6 far away from the pipe orifice of the adjusting cylinder 5, when too much nutrient solution is added, the redundant nutrient solution can drop into the collecting cup 8 through the liquid leakage pipe 6, the adding is stopped at the moment, and then the first valve is opened, so that the nutrient solution with the accurate amount can be added into the culture box 1; so that the nutrient solution with accurate amount can be added into the culture box 1.

Sealing mechanism 9 is including the section plane seal groove 91 and the sealed pad 92 that are the annular setting, and seal groove 91 is seted up on the outer section of thick bamboo wall of support section of thick bamboo 2 ring shape that is close to the top, and sealed pad 92 fixed connection is in seal groove 91, and the one end that sealed pad 92 kept away from the groove bottom of seal groove 91 passes the notch of seal groove 91 and outwards extends, and with the sealed sliding connection of inner tube wall of adjusting a section of thick bamboo 5, improves the leakproofness between adjusting a section of thick bamboo 5 and the support section of thick bamboo 2.

The top lateral wall that cultivates box 1 and is close to bracing piece one side goes up fixedly connected with magnetite 7, is provided with upper end open-ended collection cup 8 on the top lateral wall of magnetite 7, and collects cup 8's material and be iron, collects cup 8 and is used for collecting unnecessary nutrient solution.

The rotating rods 10 are symmetrically and fixedly connected to the outer barrel wall, opposite to the outer barrel wall, of the adjusting barrel 5 and located right above the liquid leakage pipe 6, and the liquid leakage pipe 6 is convenient to rotate.

Fixedly connected with base on the bottom lateral wall of cultivation box 1 for it is more stable that cultivation box 1 placed.

The top cylinder wall of the adjusting cylinder 5 is hinged with a cylinder cover to prevent external impurities from entering the adjusting cylinder 5.

According to the utility model, when a culture solution is added, the adjusting cylinder 5 is rotated according to the amount to be added, the amount of the culture solution which can be accommodated between the adjusting cylinder 5 and the supporting cylinder 2 can be changed along with the rotation of the adjusting cylinder 5, according to the position of the cylinder wall at the bottom of the adjusting cylinder 5 corresponding to the scale mark, a worker can accurately judge the amount which can be accommodated between the adjusting cylinder 5 and the supporting cylinder 2, after the adjustment is carried out to a proper position, the worker can directly add the nutrient solution between the adjusting cylinder 5 and the supporting cylinder 2, meanwhile, the collecting cup 8 is placed under the position of the leakage pipe 6 far away from the pipe orifice of the adjusting cylinder 5, when the added nutrient solution is too much, the redundant nutrient solution can drop into the collecting cup 8 through the building liquid pipe 6, at the moment, the adding is stopped, and then the first valve is opened, so that the nutrient solution with the accurate amount can be added into the culture box 1; so that the nutrient solution with accurate amount can be added into the culture box 1.

The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered to be within the technical scope of the present invention, and equivalent alternatives or modifications according to the technical solution of the present invention and the inventive concept thereof should be covered by the scope of the present invention.

Claims

1. A human oral epidermic cell culture kit for gene sequencing comprises a culture box (1) and is characterized in that the culture box (1) is made of transparent materials, three support rods are fixedly connected to the outer side wall of the top, close to the middle portion, of the culture box (1) in a matrix distribution uniform equidistant mode, a support cylinder (2) with the lower end closed is fixedly connected to the upper ends of the three support rods, the support cylinder (2) is arranged in a cylindrical mode, a liquid inlet pipe (3) is fixedly communicated with the middle portion of the outer cylinder wall at the bottom of the support cylinder (2), the lower end of the liquid inlet pipe (3) is communicated with the culture box (1), a first valve is mounted on the liquid inlet pipe (3), a circle of thread is fixedly arranged on the annular outer cylinder wall of the support cylinder (2), a scale mark is fixedly arranged on the vertical outer cylinder wall on one side of the support cylinder (2), and a nut (4) is connected to the annular outer cylinder wall of the support cylinder (2) in a threaded mode, the utility model discloses a cultivation box, including nut (4), support section of thick bamboo (2), the outer wall of one side annular outer tube wall that support section of thick bamboo (2) are close to the top is fixed with cylindric regulation section of thick bamboo (5), support section of thick bamboo (2) and install on the annular outer tube wall that is close to the top and adjust a section of thick bamboo (5) assorted sealing mechanism (9), it has weeping pipe (6) that the section is "L" type to adjust the fixed intercommunication of one side annular outer tube wall that a section of thick bamboo (5) are close to the top, and weeping pipe (6) are located and support section of thick bamboo (2) setting directly over, it has the drain pipe to cultivate fixed intercommunication on the outer wall of one side that box (1) is close to the bottom, and installs the second valve on the drain pipe.

2. The human oral epidermal cell culture kit for gene sequencing according to claim 1, wherein the sealing mechanism (9) comprises a sealing groove (91) and a sealing gasket (92), the cross section of the sealing groove (91) is annularly arranged, the sealing groove (91) is formed in the annular outer cylinder wall of the support cylinder (2) close to the top, the sealing gasket (92) is fixedly connected in the sealing groove (91), and one end, far away from the bottom of the sealing groove (91), of the sealing gasket (92) penetrates through the notch of the sealing groove (91) and extends outwards and is in sealing sliding connection with the inner cylinder wall of the adjusting cylinder (5).

3. The human oral epidermic cell culture kit for gene sequencing according to claim 1, wherein a magnet (7) is fixedly connected to the top outer side wall of one side, close to the support rod, of the culture kit (1), a collection cup (8) with an opening at the upper end is arranged on the top outer side wall of the magnet (7), and the collection cup (8) is made of iron.

4. The human oral epidermal cell culture kit for gene sequencing according to claim 1, wherein the adjusting cylinder (5) is symmetrically and fixedly connected with rotating rods (10) on the wall of the outer cylinder which is opposite to the wall of the liquid leakage pipe (6).

5. The human oral epidermal cell culture kit for gene sequencing according to claim 1, wherein a base is fixedly connected to the bottom side wall of the culture kit (1).

6. The human mouth epidermal cell culture kit for gene sequencing of claim 1, wherein a top wall of the adjusting cylinder (5) is hinged with a cylinder cover.