CN215297236U - Automatic change point and glue electrophoresis gel cutter - Google Patents
Automatic change point and glue electrophoresis gel cutter Download PDFInfo
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- CN215297236U CN215297236U CN202121309782.9U CN202121309782U CN215297236U CN 215297236 U CN215297236 U CN 215297236U CN 202121309782 U CN202121309782 U CN 202121309782U CN 215297236 U CN215297236 U CN 215297236U
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- electrophoresis
- workbench
- cutting instrument
- automatic dispensing
- gel
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Abstract
The utility model discloses an automatic dispensing electrophoresis gel cutting instrument, which relates to the field of biological experiment equipment and comprises a workbench, wherein a fixed support is arranged in the workbench, a three-shaft sliding assembly and a slide block are arranged on the support, the slide block can slide along the three-shaft sliding assembly, and an ultraviolet sterilization lamp is also arranged in the workbench; the side wall of the sliding block is also fixedly provided with a gel cutting head assembly and a visual detection device, and the bottom of the sliding block is provided with a liquid-transferring module for mounting and discharging a liquid-transferring sucker vertically arranged; the bottom of the triaxial sliding assembly is a working table used for placing materials and installing an electrophoresis tank. The utility model discloses a set up triaxial slip subassembly, realized the point and glued automation, nucleic acid runs gluey size when setting up visual detection device and being used for discerning nucleic acid formation of image, through computer algorithm, judges the size of cutting gel, cuts through gel crop subassembly, places the deep hole board in, has guaranteed safety, high efficiency and the accuracy of surely gluing.
Description
Technical Field
The utility model relates to a biological assay equipment field especially relates to an automatic change point and glue electrophoresis surely to glue appearance.
Background
Among molecular experiments, agarose gel electrophoresis is the most commonly used experimental procedure. Dyeing is needed before electrophoresis, observation is carried out under the condition of a blue light lamp, and glue is cut. In the prior art, before electrophoresis in the prior art, nucleic acid, loading buffer solution and Marker (molecular weight standard reagent) are usually added manually, a suction head needs to be replaced for each loading, and the process is repeated and tedious; after electrophoresis, manual glue cutting is needed, so that the efficiency is low and the glue cutting precision is insufficient.
SUMMERY OF THE UTILITY MODEL
To prior art not enough, the utility model aims at providing an automatic change point and glue electrophoresis surely glues appearance, it has mixed loading, point and glues, electrophoresis, surely glue integrative function to realize automated control, above-mentioned technical purpose is realized through following technical scheme:
the utility model provides an automatic change point and glue electrophoresis surely glues appearance, includes the workstation, and the closing door, its characterized in that have been seted up to the workstation side: a fixed bracket is arranged in the workbench, a three-axis sliding assembly and a sliding block are arranged on the bracket, the sliding block can transversely slide along the X axis of the three-axis sliding assembly, and an ultraviolet sterilizing lamp is also arranged in the workbench;
the side wall of the sliding block is also fixedly provided with a gel cutting head assembly and a visual detection device, the bottom of the sliding block is provided with a liquid transfer module, and the bottom of the liquid transfer module is provided with an installation joint for installing and discharging a liquid transfer suction head which is vertically arranged;
the bottom of the X shaft of the three-shaft sliding assembly is a working table top, the working table top is used for placing materials, and a mounting groove is formed in the working table top and used for mounting an electrophoresis tank.
Further: and limiting blocks are arranged on two sides of the bottom of the mounting groove and used for respectively abutting against two sides of the electrophoresis groove.
Further: the bottom of the installation groove is also fixed with two parallel and vertically arranged positioning plates which are respectively abutted against the other two sides of the electrophoresis groove, and the tops of the two positioning plates are fixedly provided with rubber supporting plates.
Further: the number of the pipetting tips is multiple.
Further: the waste material import has been seted up to the region that the workstation is used for placing the material, the waste material export has been seted up to the lateral wall of workstation, the waste material import with the waste material export passes through waste material passageway intercommunication.
Further: the waste inlet is rotatably connected with a baffle through a torsion spring.
Preferably: the shell of the workbench is a dark color acrylic plate.
Further: the electrophoresis tank is characterized in that a peristaltic pump is installed at the bottom of the electrophoresis tank, the peristaltic pump is fixedly installed on the bottom surface of the workbench, one end of the peristaltic pump is connected with the electrophoresis tank, and the other end of the peristaltic pump is connected with an electrophoresis tank arranged outside the workbench.
The utility model has the advantages that: through setting up triaxial sliding assembly, realized the point and glued automation, saved the manual work, simultaneously, set up the size that vision detection device was used for discerning when nucleic acid runs to glue formation of image, through computer algorithm, judge the size of cutting gel, rethread gel crop subassembly cuts, has guaranteed the efficiency and the accuracy nature of surely gluing.
Drawings
Fig. 1 is a schematic structural diagram of the present invention;
FIG. 2 is a schematic view of the installation of the electrophoresis tank;
fig. 3 is a process flow diagram of the present invention.
In the figure, 1, a workbench; 2. a slider; 21. pipetting tips; 3. a gel crop assembly; 4. a visual inspection device; 5. an electrophoresis tank; 6. a limiting block; 7. a work table; 8. positioning a plate; 9. a waste inlet; 91. A baffle plate; 10. a waste outlet; 11. and (7) supporting the rubber plate.
Detailed Description
In order to make the technical means, creation features, achievement purposes and functions of the present invention easy to understand, the present invention is further described below with reference to the following embodiments.
In the description of the present invention, it is to be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "disposed," "connected," and the like are to be construed broadly, and for example, "connected" may be either fixedly connected or detachably connected, or integrally connected; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meaning of the above terms in the present invention can be understood in specific cases to those skilled in the art.
As shown in figure 1, the automatic dispensing electrophoresis gel cutting instrument comprises a workbench 1, wherein the workbench 1 is preferably an ultra-clean workbench or a biological safety cabinet to provide air purification of local dustless and sterile working environment, polluted air in a working area can be artificially controlled and discharged through a special filtering channel, harm to people and the environment is avoided, and a closing door is arranged on the side surface of the workbench 1 and can be opened or closed.
Be equipped with the fixed bolster in the workstation 1, install triaxial sliding assembly and slider 2 on the support, slider 2 sets up in the horizontal fixed X of triaxial sliding assembly epaxially, still install the ultraviolet sterilamp in the workstation 1. In order to ensure the sterilization effect of the ultraviolet sterilization lamp and not affect the external environment of the worktable 1, the casing of the worktable 1 is preferably made of dark acrylic plate material.
Still include gel crop subassembly 3, gel crop subassembly 3 sets firmly in the lateral wall of slider 2 including being used for the cutter of surely gluing, its one side, and the opposite side links to each other with visual detection device 4, and the liquid-transfering module is installed to 2 bottoms of slider, for avoiding cross contamination, moves liquid module bottom and is equipped with the erection joint for the liquid-transfering suction head 21 of installation and the vertical setting of discharge to change liquid-transfering suction head 21. The utility model discloses preferred installation joint quantity is 8, can move the liquid simultaneously. The flexible setting of erection joint, during the installation, the erection joint stretches out, and the vertical cup joint of liquid-transfering suction head 21 is outside at the erection joint, and when needing to be changed liquid-transfering suction head 21, liquid-transfering suction head 21 is inwards shrink, and liquid-transfering suction head 21 drops naturally, and this operating procedure is accomplished by controller control automation. As another embodiment, a rotating body capable of rotating in a vertical plane is arranged in the slide block 2, the rotating body is prismatic, each side surface of the rotating body is provided with a plurality of mounting joints, the bottom pipetting tip 21 can be replaced by rotating the rotating body, and the complicated step that the pipetting tip 21 needs to be replaced every time is omitted. Visual detection device 4 includes the camera of being connected with the controller for collect the sampling, size position when discernment nucleic acid formation of image through computer algorithm, judges the size of cutting the gel, and rethread gel crop subassembly cuts, in order to guarantee the precision degree of cutting the gel.
The bottom of the triaxial sliding assembly is a working table surface 7, the working table surface 7 is used for placing materials, and the installation groove is formed for installing the electrophoresis tank 5.
As shown in fig. 2, two sides of the bottom of the installation groove are provided with a plurality of limiting blocks 6, preferably an even number of limiting blocks 6, in this embodiment, the number of limiting blocks 6 is preferably 4, and one side of each limiting block 6 is abutted to two sides of the electrophoresis groove 5.
In order to prevent cross contamination, the electrophoresis liquid needs to be replaced when running glue, therefore, a peristaltic pump is arranged below the electrophoresis tank 5, an electrophoresis liquid tank (not shown in the figure) is arranged outside the workbench 1, the peristaltic pump is fixed on the bottom surface of the workbench 1, one end of the peristaltic pump is connected with the electrophoresis tank 5, the other end of the peristaltic pump is connected with the electrophoresis liquid tank, and the electrophoresis liquid in the electrophoresis liquid tank can be pumped into the electrophoresis tank 5 to realize liquid replacement.
As a further optimization, a waste inlet 9 is formed in the area, used for placing materials, of the working table top 7, a waste outlet 10 is formed in the side wall of the working table 1, and the waste inlet 9 and the waste outlet 10 are communicated through a waste channel. Waste material import 9 department is connected with baffle 91 through the torsional spring rotation, and waste pipetting module 21 drops to waste material import 9, in order to prevent that the remaining liquid in pipetting module 21 from causing aerosol cross contamination, sets up baffle 91 and plays the guard action, because the torsional spring effect, pipetting module 21 after dropping baffle 91 resumes original position.
For the convenience of moving, the bottom of the workbench 1 is uniformly provided with a plurality of universal wheels along the circumferential direction, which are not shown in the figure.
The utility model discloses a triaxial slip subassembly, gel crop subassembly 3, visual detection device 4, move the erection joint etc. of liquid module bottom all through controller automatic control to realize automated control, control mode is conventional technological means, and here does not make detailed description.
The work flow of the utility model is as shown in figure 3:
s1, manually placing the required materials to the designated position of a working table top 7 in sequence, connecting one end of a peristaltic pump with an electrophoresis tank, connecting the other end of the peristaltic pump with the electrophoresis tank 5, and then manually placing the prepared gel.
S2, closing the closing door, operating the equipment, and paying attention to the fact that the equipment cannot operate when the door is not closed.
S3, the motor drives the sliding block 2 to move to the upper portion of the area of the electrophoresis tank 5, the visual detection device 4 shoots the gel hole at the position of the electrophoresis tank 5, and after data collection is finished, the peristaltic pump runs to pump electrophoresis liquid into the electrophoresis tank 5.
S4, moving the slide block 2 to a material area, pipetting to a gel hole, and repeating pipetting until the end is reached. The side-labeled assembly was then pipetted at the designated area of the gel.
And S5, after the liquid transfer is finished, the electrophoresis tank 5 works to run glue until the glue transfer is finished.
S6, after the glue is completely run, the liquid in the electrophoresis tank 5 is pumped into the electrophoresis tank by a peristaltic pump and is cooled. The lamp plate in the workstation 1 shines the gel lamp, and visual detection device 4 shoots the data acquisition directly over.
And S7, after data comparison, the machine sliding block 2 drives the gel cutting head component 3 to move, gel is cut for many times in a specified area and is placed in a storage deep hole plate until the end.
And S8, after the operation is finished, giving an alarm for prompting.
The basic principles and the main features of the invention and the advantages of the invention have been shown and described above. It will be understood by those skilled in the art that the present invention is not limited to the above embodiments, and that the foregoing embodiments and descriptions are provided only to illustrate the principles of the present invention without departing from the spirit and scope of the present invention. The scope of the invention is defined by the appended claims and equivalents thereof.
Claims (8)
1. The utility model provides an automatic change point and glue electrophoresis surely glues appearance, includes workstation (1), and the closing door, its characterized in that have been seted up to workstation (1) side: a fixed bracket is arranged in the workbench (1), a three-axis sliding assembly and a sliding block (2) are arranged on the bracket, the sliding block (2) can transversely slide along the X axis of the three-axis sliding assembly, and an ultraviolet sterilization lamp is further arranged in the workbench (1);
the side wall of the sliding block (2) is also fixedly provided with a gel crop assembly (3) and a visual detection device (4), the bottom of the sliding block (2) is provided with a liquid transfer module, and the bottom of the liquid transfer module is provided with a mounting joint for mounting and discharging a liquid transfer suction head (21) which is vertically arranged;
the X-axis bottom of the three-axis sliding assembly is a workbench surface (7), the workbench surface (7) is used for placing materials, and an installation groove is formed in the workbench surface (7) and used for installing the electrophoresis tank (5).
2. The automatic dispensing electrophoresis gel cutting instrument of claim 1, wherein: and limiting blocks (6) are arranged on two sides of the bottom of the mounting groove and are used for abutting against two sides of the electrophoresis groove (5) respectively.
3. The automatic dispensing electrophoresis gel cutting instrument of claim 2, wherein: the bottom of the installation groove is also fixed with two parallel and vertically arranged positioning plates (8) which are respectively abutted against the other two sides of the electrophoresis groove (5), and the tops of the two positioning plates (8) are fixedly provided with rubber supporting plates (11).
4. The automatic dispensing electrophoresis gel cutting instrument of claim 1, wherein: the number of the pipetting tips (21) is multiple.
5. The automatic dispensing electrophoresis gel cutting instrument of claim 1, wherein: the waste material inlet (9) has been seted up in the region that table surface (7) are used for placing the material, waste material export (10) have been seted up to the lateral wall of workstation (1), waste material import (9) with waste material export (10) are through waste material passageway intercommunication.
6. The automatic dispensing electrophoresis gel cutting instrument of claim 5, wherein: the waste inlet (9) is rotatably connected with a baffle (91) through a torsion spring.
7. The automatic dispensing electrophoresis gel cutting instrument of claim 1, wherein: the shell of the workbench (1) is a dark color acrylic plate.
8. The automatic dispensing electrophoresis gel cutting instrument of claim 1, wherein: the electrophoresis tank is characterized in that a peristaltic pump is installed at the bottom of the electrophoresis tank (5), the peristaltic pump is fixedly installed on the bottom surface of the workbench (1), one end of the peristaltic pump is connected with the electrophoresis tank (5), and the other end of the peristaltic pump is connected with the electrophoresis tank.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202121309782.9U CN215297236U (en) | 2021-06-11 | 2021-06-11 | Automatic change point and glue electrophoresis gel cutter |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202121309782.9U CN215297236U (en) | 2021-06-11 | 2021-06-11 | Automatic change point and glue electrophoresis gel cutter |
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| CN215297236U true CN215297236U (en) | 2021-12-24 |
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| CN202121309782.9U Active CN215297236U (en) | 2021-06-11 | 2021-06-11 | Automatic change point and glue electrophoresis gel cutter |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114397348A (en) * | 2021-12-28 | 2022-04-26 | 苏州中析生物信息有限公司 | Full-automatic all-in-one of liquid-transfering electrophoresis |
-
2021
- 2021-06-11 CN CN202121309782.9U patent/CN215297236U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114397348A (en) * | 2021-12-28 | 2022-04-26 | 苏州中析生物信息有限公司 | Full-automatic all-in-one of liquid-transfering electrophoresis |
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