CN214991565U - Nanopore sequencing experiment table - Google Patents
Nanopore sequencing experiment table Download PDFInfo
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- CN214991565U CN214991565U CN202120066582.9U CN202120066582U CN214991565U CN 214991565 U CN214991565 U CN 214991565U CN 202120066582 U CN202120066582 U CN 202120066582U CN 214991565 U CN214991565 U CN 214991565U
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- 238000007672 fourth generation sequencing Methods 0.000 title claims abstract description 36
- 238000002474 experimental method Methods 0.000 title claims abstract description 11
- 239000007788 liquid Substances 0.000 claims abstract description 65
- 238000006073 displacement reaction Methods 0.000 claims abstract description 49
- 239000000758 substrate Substances 0.000 claims abstract description 9
- 238000000034 method Methods 0.000 claims abstract description 8
- 239000011521 glass Substances 0.000 claims abstract description 5
- 230000005540 biological transmission Effects 0.000 claims description 6
- 230000003287 optical effect Effects 0.000 claims description 3
- 238000001514 detection method Methods 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 239000011324 bead Substances 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 238000009434 installation Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 210000000080 chela (arthropods) Anatomy 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- 230000000750 progressive effect Effects 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000012163 sequencing technique Methods 0.000 description 1
- 230000000007 visual effect Effects 0.000 description 1
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Abstract
The utility model discloses a nanopore sequencing experiment table, include: a base; the whole machine fixing base body is arranged on the base, a lower liquid pool is fixed on a substrate at the top end of the whole machine fixing base body, and a glass slide is arranged on the lower liquid pool; the three-dimensional precision displacement platform is arranged on a whole machine fixing base body, a beam of the three-dimensional precision displacement platform is connected with an upper liquid pool, the piezoelectric actuator is arranged on the three-dimensional precision displacement platform and used for driving the beam of the three-dimensional precision displacement platform to move up and down along a Z axis, the upper liquid pool is positioned above a lower liquid pool, and a chip provided with a nano hole is fixed on the upper liquid pool; and the inverted microscope is arranged on the base in an inverted mode and is used for observing the whole experimental process, so that the observation of the condition that the single molecular chain passes through the nano hole is realized. The utility model discloses a single molecular chain passes the accurate control of nanopore.
Description
Technical Field
The utility model relates to a gene detection technical field, more specifically say, relate to a nanopore sequencing laboratory bench.
Background
Magnetic tweezers are a micro-manipulation technology developed in recent years and used in the field of life science research, and the magnetic field controls the movement of superparamagnetic beads, and the beads are used for capturing single molecules, so that a series of subsequent mechanical stretching experiments are performed. However, at present, the situation of the single molecular chain passing through the nanopore cannot be observed, and the single molecular chain passing through the nanopore cannot be precisely controlled.
In summary, how to observe the situation of the single molecular chain passing through the nanopore and precisely control the single molecular chain passing through the nanopore is a problem to be solved by those skilled in the art.
SUMMERY OF THE UTILITY MODEL
In view of this, the present invention is directed to a nanopore sequencing bench, which can observe the situation that a single molecular chain passes through a nanopore, and precisely control the single molecular chain to pass through the nanopore.
In order to achieve the above object, the utility model provides a following technical scheme:
a nanopore sequencing bench comprising:
a base;
the whole machine fixing base body is arranged on the base, a lower liquid pool is fixed on a substrate at the top end of the whole machine fixing base body, and a glass slide for connecting a molecular chain to be sequenced is arranged on the lower liquid pool;
the three-dimensional precision displacement platform is arranged on the whole machine fixing base body, a beam of the three-dimensional precision displacement platform is connected with an upper liquid pool, the piezoelectric actuator is arranged on the three-dimensional precision displacement platform and used for driving the beam of the three-dimensional precision displacement platform to move up and down along a Z axis, the upper liquid pool is positioned above the lower liquid pool, and a chip provided with a nano hole is fixed on the upper liquid pool;
and the inverted microscope is inversely arranged on the base and is used for observing the whole experimental process.
In a specific embodiment, the base is an optical platform.
In another specific embodiment, the nanopore sequencing bench further comprises a lower locating plate;
the lower positioning plate is arranged on the base plate and used for positioning the lower liquid pool.
In another specific embodiment, the nanopore sequencing bench further comprises an upper positioning plate;
the upper positioning plate is arranged on a beam of the three-dimensional precision displacement table and used for positioning the upper liquid pool.
In another specific embodiment, the nanopore sequencing bench further comprises a first shielding cartridge;
and the first shielding box cover is arranged outside the upper liquid pool and the lower liquid pool and is connected with the three-dimensional precision displacement table.
In another specific embodiment, the nanopore sequencing bench further comprises a second shielding box;
the second shielding box is covered outside the first shielding box, is connected with the three-dimensional precise displacement table and is used for covering the patch clamp on the whole machine fixing base body.
In another specific embodiment, the first shielding box is a faraday cage.
In another specific embodiment, the second shielding box is a faraday cage.
In another specific embodiment, the nanopore sequencing bench further comprises an X-axis drive screw;
the X-axis driving screw is installed on the three-dimensional precise displacement table and is in transmission connection with a cross beam of the three-dimensional precise displacement table.
In another specific embodiment, the nanopore sequencing bench further comprises a Y-axis drive screw;
and the Y-axis driving screw is arranged on the three-dimensional precise displacement table and is in transmission connection with a cross beam of the three-dimensional precise displacement table.
According to the utility model discloses an each embodiment can make up as required wantonly, and the embodiment that obtains after these combinations is also in the utility model discloses the scope is the utility model discloses a part of the concrete implementation mode.
According to the technical scheme, in the nanopore sequencing experiment table provided by the utility model, when in use, a molecular chain to be sequenced is fixed on a glass sheet of a lower liquid pool, and a chip provided with a nanopore is fixed on an upper liquid pool; then, fixing a lower liquid pool on a substrate of a whole machine fixing base body, and fixing an upper liquid pool on a beam of a three-dimensional precision displacement table; then, operating the three-dimensional precision displacement table to enable the beam to drive the upper liquid pool to move to the position right above the lower liquid pool; and then, the piezoelectric actuator drives the beam to drive the upper liquid pool to accurately move along the Z axis, so that the molecular chain to be sequenced passes through the nanopore. The utility model provides a nanopore sequencing laboratory bench has realized that the single molecular chain passes the accurate control of nanopore.
Furthermore, the utility model discloses set up the inverted microscope, can observe whole test process, and then realized the observation to the condition that the single molecular chain passed the nanopore.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to these drawings without creative efforts.
Fig. 1 is a schematic diagram of a three-dimensional structure of a nanopore sequencing experiment table provided by the present invention;
FIG. 2 is a schematic front view of the structure of FIG. 1;
FIG. 3 is a schematic top view of the structure of FIG. 1;
fig. 4 is a schematic diagram of a local three-dimensional structure of a nanopore sequencing experiment table provided by the present invention;
FIG. 5 is a schematic front view of the structure of FIG. 4;
fig. 6 is a schematic top view of the structure of fig. 4.
In fig. 1-6:
the device comprises a base 1, a whole machine fixing base body 2, a base plate 201, a lower liquid pool 3, a three-dimensional precision displacement table 4, a cross beam 401, a piezoelectric actuator 5, an upper liquid pool 6, a lower positioning plate 7, an upper positioning plate 8, a second shielding box 9, a diaphragm clamp 10, an inverted microscope 11, an X-axis driving screw 12 and a Y-axis driving screw 13.
Detailed Description
The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.
In the description of the present invention, it should be understood that the terms "upper", "lower", "top surface", "bottom surface", etc. indicate the orientation or positional relationship based on the orientation or positional relationship shown in the drawings, which is only for convenience of description and simplification of description, and does not indicate or imply that the position or element referred to must have a specific orientation, be constituted in a specific orientation, and be operated, and thus, should not be construed as limiting the present invention. Furthermore, the terms "first" and "second" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.
Referring to fig. 1-6, the present invention provides a nanopore sequencing bench, wherein the nanopore sequencing bench includes a base 1, a whole machine fixing base 2, a three-dimensional precision displacement stage 4, a piezoelectric actuator 5, and an inverted microscope 11.
The complete machine fixing base body 2 is installed on the base 1, and specifically, the complete machine fixing base body 2 is detachably installed on the base 1 through bolts and the like. A plurality of bolt holes are uniformly distributed on the base 1, so that the position of the whole machine fixing base body 2 can be conveniently adjusted.
The whole machine fixing base body 2 comprises a support and a base plate 201, the base plate 201 comprises an L-shaped plate and a horizontal plate, the horizontal plate is detachably connected with the vertical end of the L-shaped plate, the horizontal plate is located above the L-shaped plate, and the horizontal ends of the horizontal plate and the L-shaped plate are respectively connected with the support.
The lower liquid pool 3 is fixed on a horizontal plate of the substrate 201, and a slide is arranged on the lower liquid pool 3 and is used for connecting a molecular chain to be tested. The molecular chain to be sequenced is a single molecular chain such as DNA.
The three-dimensional precision displacement table 4 is mounted on the complete machine fixing base body 2, and specifically, the three-dimensional precision displacement table 4 is detachably connected to the horizontal end of the L-shaped plate of the substrate 201 through bolts and the like.
The beam 401 of the three-dimensional precision displacement table 4 is connected with the upper liquid pool 6, and specifically, the upper liquid pool 6 is detachably connected to the beam 401 through bolts and the like.
And the piezoelectric actuator 5 is arranged on the three-dimensional precision displacement table 4 and is used for driving the beam 401 of the three-dimensional precision displacement table 4 to move up and down along the Z axis so as to realize the accurate control of up and down movement of the nanopore. Adopt electronic piezoelectric actuator 5 drive crossbeam 401 to reciprocate and satisfy visual demand, easy operation, the control of being convenient for is fit for experimental use.
The upper liquid pool 6 is positioned above the lower liquid pool 3, and a chip provided with a nanopore is fixed on the upper liquid pool 6.
The inverted microscope 11 is arranged on the base 1 in an inverted mode and used for observing the whole experimental process, specifically, an eyepiece of the inverted microscope 11 is located below the upper liquid pool 6 and can observe the position of the nanopore, and the piezoelectric actuator 5 and the three-dimensional precise displacement table 4 can be conveniently controlled to move until a molecular chain to be detected passes through the nanopore.
In the nanopore sequencing experiment table provided by the utility model, when in use, a molecular chain to be sequenced is fixed on a glass sheet of the lower liquid pool 3, and a chip provided with a nanopore is fixed on the upper liquid pool 6; then, fixing the lower liquid pool 3 on the substrate 201 of the whole machine fixing base body 2, and fixing the upper liquid pool 6 on a cross beam 401 of the three-dimensional precision displacement table 4; then, the three-dimensional precision displacement table 4 is controlled, so that the beam 401 can drive the upper liquid pool 6 to move right above the lower liquid pool 3; then, the piezoelectric actuator 5 drives the beam 401 to drive the upper liquid pool 6 to precisely move along the Z axis, so that the molecular chain to be sequenced passes through the nanopore. The utility model provides a nanopore sequencing laboratory bench has realized that the single molecular chain passes the accurate control of nanopore.
Furthermore, the utility model discloses set up inverted microscope 11, can observe whole test process, and then realized the observation to the condition that the single molecular chain passed the nanopore.
Specifically, the objective lens of the inverted microscope 11 is a 40X objective lens, the focal length is 2.2mm, the design distance from the lower surface of the upper liquid pool 6 to the upper mirror surface of the objective lens is 2.2mm, the distance from the lower surface of the upper liquid pool 6 to the lower surface of the lower liquid pool 3 is 1.7mm, the distance from the upper mirror surface of the objective lens of the inverted microscope 11 to the bottom surface (i.e. on the top end surface of the base 1) is greater than or equal to 190mm and less than or equal to 210mm, and the effective distance between the nanopore on the chip and the slide is controlled within 3 μm for up-and-down movement.
In some embodiments, the utility model discloses a nanopore sequencing laboratory bench still includes X axle driving screw 12, and X axle driving screw 12 installs on three-dimensional accurate displacement platform 4, and is connected with the crossbeam 401 transmission of three-dimensional accurate displacement platform 4 for through the displacement of X axle driving screw 12 manual regulation crossbeam 401 along the X axle direction, and then realize going up the position control of liquid bath 6 along the X axle direction.
Further, the utility model discloses a nanopore sequencing laboratory bench still includes Y axle drive screw 13, and Y axle drive screw 13 is installed on three-dimensional accurate displacement platform 4, and is connected with the crossbeam 401 transmission of three-dimensional accurate displacement platform 4 for through the displacement of Y axle drive screw 13 manual regulation crossbeam 401 along the Y axle direction, and then realize going up the position control of liquid bath 6 along the Y axle direction.
In some embodiments, the utility model discloses a base 1 is optical platform, has guaranteed the roughness of base 1 mesa and the stationarity of base 1, provides the basis for going on of sequencing experiment specifically.
In some embodiments, the nanopore sequencing bench further comprises a lower positioning plate 7, as shown in fig. 4-6, the lower positioning plate 7 being mounted on the base plate 201 for positioning the lower reservoir 3 to facilitate the installation of the lower reservoir 3 in place.
Further, the utility model discloses a nanopore sequencing laboratory bench still includes locating plate 8, and locating plate 8 is installed on the crossbeam 401 of three-dimensional accurate displacement platform 4 for the liquid bath 6 in the location, so that the installation of liquid bath 6 targets in place.
In some embodiments, the nanopore sequencing bench further comprises a first shielding box, which covers the upper liquid pool 6 and the lower liquid pool 3 and is connected with the three-dimensional precision displacement stage 4. Specifically, the first shielding box can be detachably mounted on the horizontal plate of the substrate 201 of the three-dimensional precision displacement table 4 through screws, so that the external interference on the working processes of the upper liquid pool 6 and the lower liquid pool 3 is avoided.
Further, the utility model discloses a nanopore sequencing laboratory bench still includes second shielding box 9, and second shielding box 9 covers to be established outside first shielding box, and is connected with three-dimensional accurate displacement platform 4 for the diaphragm pincers 10 on the complete machine fixed base member 2 are established to the cover. Specifically, the second shielding box 9 can be detachably mounted on the horizontal plate of the substrate 201 of the three-dimensional precision displacement table 4 through screws, so that the patch clamp 10 is prevented from being interfered by the outside world in the working process.
The two ends of the patch clamp 10 are respectively connected with the electrodes on the two sides of the nanopore, and the patch clamp has the functions of outputting voltage and detecting micro current. Because the channel of the liquid on the two sides of the nanopore is only the nanopore, the solutions of the upper liquid pool 6 and the lower liquid pool 3 have certain conductivity, when voltage exists between the liquids on the two sides, a tiny current can pass through the nanopore, and the current detection of the patch clamp 10 can detect the current. The magnitude of the current is related to the conduction area of the pore, and the like, so when a molecular chain to be sequenced penetrates into the nanopore, the channel is reduced (the resistance is increased), and the current is reduced. According to this principle, the molecular chain to be sequenced can be judged to penetrate into the nanopore from the detection signal of the patch clamp 10.
In some embodiments, the first shielding box and the second shielding box 9 are faraday cages, and it should be noted that the first shielding box and the second shielding box 9 can be made of other shielding materials.
The embodiments in the present description are described in a progressive manner, each embodiment focuses on differences from other embodiments, and the same and similar parts among the embodiments are referred to each other.
The previous description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the invention. Thus, the present invention is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.
In the description herein, references to the description of "one embodiment," "an example," "a specific example," etc., mean that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the invention. In this specification, the schematic representations of the terms used above do not necessarily refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.
The preferred embodiments of the present invention disclosed above are intended only to help illustrate the present invention. The preferred embodiments are not intended to be exhaustive or to limit the invention to the precise embodiments disclosed. Obviously, many modifications and variations are possible in light of the above teaching. The embodiments were chosen and described in order to best explain the principles of the invention and its practical applications, to thereby enable others skilled in the art to best understand the invention for and utilize the invention. The present invention is limited only by the claims and their full scope and equivalents.
Claims (10)
1. A nanopore sequencing laboratory bench, comprising:
a base;
the whole machine fixing base body is arranged on the base, a lower liquid pool is fixed on a substrate at the top end of the whole machine fixing base body, and a glass slide for connecting a molecular chain to be sequenced is arranged on the lower liquid pool;
the three-dimensional precision displacement platform is arranged on the whole machine fixing base body, a beam of the three-dimensional precision displacement platform is connected with an upper liquid pool, the piezoelectric actuator is arranged on the three-dimensional precision displacement platform and used for driving the beam of the three-dimensional precision displacement platform to move up and down along a Z axis, the upper liquid pool is positioned above the lower liquid pool, and a chip provided with a nano hole is fixed on the upper liquid pool;
and the inverted microscope is inversely arranged on the base and is used for observing the whole experimental process.
2. The nanopore sequencing laboratory bench of claim 1, wherein the base is an optical platform.
3. The nanopore sequencing laboratory bench of claim 1, further comprising a lower positioning plate;
the lower positioning plate is arranged on the base plate and used for positioning the lower liquid pool.
4. The nanopore sequencing experiment table according to claim 3, further comprising an upper positioning plate;
the upper positioning plate is arranged on a beam of the three-dimensional precision displacement table and used for positioning the upper liquid pool.
5. The nanopore sequencing laboratory bench of claim 4, further comprising a first shielding box;
and the first shielding box cover is arranged outside the upper liquid pool and the lower liquid pool and is connected with the three-dimensional precision displacement table.
6. The nanopore sequencing laboratory bench of claim 5, further comprising a second shielding box;
the second shielding box is covered outside the first shielding box, is connected with the three-dimensional precise displacement table and is used for covering the patch clamp on the whole machine fixing base body.
7. The nanopore sequencing laboratory table of claim 6, wherein said first shielding box is a Faraday cage.
8. The nanopore sequencing laboratory bench of claim 7, wherein said second shielding box is a Faraday cage.
9. The nanopore sequencing laboratory bench of any one of claims 1-8, further comprising an X-axis drive screw;
the X-axis driving screw is installed on the three-dimensional precise displacement table and is in transmission connection with a cross beam of the three-dimensional precise displacement table.
10. The nanopore sequencing experiment table of claim 9, further comprising a Y-axis drive screw;
and the Y-axis driving screw is arranged on the three-dimensional precise displacement table and is in transmission connection with a cross beam of the three-dimensional precise displacement table.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202120066582.9U CN214991565U (en) | 2021-01-11 | 2021-01-11 | Nanopore sequencing experiment table |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202120066582.9U CN214991565U (en) | 2021-01-11 | 2021-01-11 | Nanopore sequencing experiment table |
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| Publication Number | Publication Date |
|---|---|
| CN214991565U true CN214991565U (en) | 2021-12-03 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202120066582.9U Expired - Fee Related CN214991565U (en) | 2021-01-11 | 2021-01-11 | Nanopore sequencing experiment table |
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|---|---|
| CN (1) | CN214991565U (en) |
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2021
- 2021-01-11 CN CN202120066582.9U patent/CN214991565U/en not_active Expired - Fee Related
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| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20211203 |
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| CF01 | Termination of patent right due to non-payment of annual fee |