CN214830397U - Cell culture plate for cell immunofluorescence - Google Patents

Abstract

The utility model discloses a cell culture board for cell immunofluorescence, including the culture plate support, cultivate the cell, cover glass, culture plate lid, the culture plate support divides 96 holes, 48 holes and three kinds of models in 24 holes, cultivates the cell and divides 96 holes, 48 holes and three kinds of models in 24 holes, and the cover glass divides 96 holes, 48 holes and three kinds of models in 24 holes, the utility model discloses a cultivate the cell bottom and can directly be used for immunofluorescence experiment and follow-up confocal microscope fluorescence observation after the cell planking is cultivateed for high printing opacity slide and through polylysine processing, and cultivate the cell and arrange the mode for detachable construction, can further improve the convenience of experiment.

Description

Cell culture plate for cell immunofluorescence

Technical Field

The utility model belongs to the technical field of laboratory cell culture technique and specifically relates to a cell culture plate for cell immunofluorescence.

Background

The cell culture plate is widely applied to various cell cultures, the cell culture plate is used for cell culture and cell function detection in a laboratory, and in a cell immunofluorescence experiment, an experimenter often uses polylysine of various specifications of cover glass to coat the cover glass and then places the cover glass on various cell culture plates in various models to perform cell plating and cell slide climbing, and then takes out the cover glass and the cell slide climbing to perform a subsequent staining experiment. The existing cell culture plate can not be directly used for cell immunofluorescence experiments and subsequent fluorescence confocal observation.

SUMMERY OF THE UTILITY MODEL

The utility model aims at: the cell culture plate for cell immunofluorescence is provided, polylysine coated slide climbing is not needed, cells can be directly plated and then subjected to subsequent immunofluorescence staining in the culture plate, and the culture plate can be adapted to a confocal microscope and can be directly subjected to fluorescence observation.

In order to achieve the above object, the utility model adopts the following technical scheme: a cell culture plate for cell immunofluorescence comprises a culture plate support, a culture chamber cover glass and a culture plate cover, wherein the culture plate support comprises a frame and a support frame, the frame is arranged on the periphery of the support frame, the support frame comprises a transverse plate and a plurality of vertical supports, the vertical supports are arranged at the bottom end of the transverse plate, the plurality of vertical supports are uniformly distributed in a cross mode along the transverse direction and the longitudinal direction of the transverse plate, a plurality of small compartments are defined by the transverse plate, the vertical supports and the frame, circular holes are formed in the transverse plate of each small compartment, the support frame comprises 96 holes, 48 holes and 24 holes according to the number of the circular holes to adapt to culture chambers of different models, each culture chamber is a cylindrical chamber, an epitaxial plate is horizontally arranged on the edge of the top of each culture chamber, a lower flange is arranged at the tail end of the epitaxial plate, an edge clamping groove which is concentric with the circular holes is arranged at the upper end of the transverse plate, the lower flange of every cultivation cell is dismantled alone and is fixed and use on the support frame through marginal draw-in groove, and the bottom of cultivateing the cell is the high printing opacity slide that has handled through polylysine, and the cover slip is high printing opacity slide, covers the top of cultivateing the cell, cultivates board lid adaptation and cultivates the board support and covers and locate the cultivation outside of board support.

Preferably, the coverslip includes 96-well, 48-well and 24-well diameter sizes to fit different models of culture chambers.

Preferably, the support frames corresponding to the 96-hole support frame, the 48-hole support frame and the 24-hole support frame are distributed into 11 transverse support frames and 7 longitudinal support frames; 7 in the transverse direction and 5 in the longitudinal direction; 5 pieces in the transverse direction and 3 pieces in the longitudinal direction.

Preferably, the culture chamber further comprises a cylindrical side wall, the high-light-transmittance slide is arranged at the bottom of the side wall, the epitaxial plate is arranged at the top of the side wall, and the cover glass is arranged on the epitaxial plate in an attaching mode.

Preferably, the cover glass is set to be a convex lens structure, the convex lens is arranged at the lower end of the cover glass, and the diameter size of the convex lens is the same as that of the inner space of the culture chamber, so that the cover glass can be well limited, and the cover glass is prevented from falling off accidentally.

The utility model discloses beneficial effect does:

1) because the bottom of the culture chamber of the utility model is made of high-transparency glass rather than common plastic, the culture chamber has stronger light transmittance, can be adapted to a confocal microscope culture plate frame, and can directly carry out subsequent immunofluorescence experiments and subsequent confocal microscope fluorescence observation after cell plating;

2) the culture chamber of the utility model is arranged on the culture plate bracket 1 through the lower flange clamping, thereby the culture chamber is of a detachable structure, and the detachable structure improves the convenience during experimental operation;

3) the utility model discloses a cover glass, consequently, improved cell culture's security, simultaneously for cover glass is convex lens form, when guaranteeing that the firm lid of cover glass locates to cultivate the cell, can conveniently observe the cell of cultivateing in the cell 7 more, and microscopical magnification can be reduced in convex lens's use.

Drawings

FIG. 1 is a schematic view showing the overall structure of a culture plate for cellular immunofluorescence;

FIG. 2 is a cross-sectional view A-A of FIG. 1;

FIG. 3 is another embodiment of a cover slip of the cross-sectional view A-A of FIG. 1;

FIG. 4 is a view from below looking up the support;

FIG. 5 is a top view of the cover slip.

Description of the reference symbols

1. A culture plate support; 2. a frame; 3. a support frame; 4. a transverse plate; 5. vertical support; 6. a side wall; 7. culturing the chamber; 8. an epitaxial plate; 9. a lower flange; 10. a convex mirror; 11. an edge slot; 12. a high light transmission slide; 13. a cover glass; 14. a circular hole; 15. and (5) covering the culture plate.

Detailed Description

The following describes a mode for carrying out the present invention with reference to the drawings.

As shown in FIGS. 1-2 and 4, a cell culture plate for cellular immunofluorescence comprises a culture plate support 1, culture chambers 7, a cover glass 13 and a culture plate cover 15, wherein the culture plate support 1 comprises a frame 2 and a support frame 3, the frame 2 is arranged on the periphery of the support frame 3, the support frame 3 comprises a transverse plate 4 and a plurality of vertical supports 5, the vertical supports 5 are arranged at the bottom end of the transverse plate 4, the plurality of vertical supports 5 are uniformly and crossly distributed along the transverse direction and the longitudinal direction of the transverse plate 4, a plurality of small compartments are defined by the transverse plate 4, the vertical supports 5 and the frame 2, circular holes 14 are arranged on the transverse plate 4 of each small compartment, according to the number of the circular holes 14, the support frame 3 comprises three types of 96 holes, 48 holes and 24 holes (the 48 holes are exemplified in FIG. 4) to be matched with the culture chambers 7 of different types, the culture chambers 7 are cylindrical chambers, an extension plate 8 is horizontally arranged on the top edge of the culture chambers 7, the end of epitaxial plate 8 is provided with flange 9 down, be provided with the edge draw-in groove 11 with the concentric setting of circular port 14 in transverse plate 4 upper end, every lower flange 9 of cultivateing cell 7 is according to the experiment needs through edge draw-in groove 11 dismantlement alone and fix and use on support frame 3, and the bottom of cultivateing cell 7 is the high printing opacity slide 12 that has handled through polylysine, cover glass 13 is the high printing opacity slide, the top of cultivateing cell 7 is located to the lid, cultivate board support 1 and the lid is located and cultivates board support 1 outside to cultivation board lid 15 adaptation.

Preferably, the coverslip 13 includes 96-well, 48-well and 24-well diameters sized to fit different types of culture chambers.

Preferably, the number of the support frames 3 corresponding to the three types of 96-hole support frames 3, 48-hole support frames 3 and 24-hole support frames 3 is 11 in the transverse direction and 7 in the longitudinal direction; 7 in the transverse direction and 5 in the longitudinal direction; 5 pieces in the transverse direction and 3 pieces in the longitudinal direction.

Preferably, as shown in FIG. 2, the culture chamber 7 further comprises a cylindrical side wall 6, a high light-transmitting slide 12 is disposed on the bottom of the side wall 6, an epitaxial plate 8 is disposed on the top of the side wall 6, and a cover glass 13 is attached to the epitaxial plate 8.

Preferably, as shown in fig. 3, in order to facilitate the cover of the cover glass 13 to be placed on the culture chamber 7 and to facilitate the observation of the cells in the culture chamber 7, the cover glass 13 is made to be a convex lens structure, and the convex lens 10 is placed at the lower end of the cover glass 13, the diameter of the convex lens 10 is the same as the diameter of the inner space of the culture chamber 7, so as to limit the cover glass 13 well and prevent the cover glass 13 from falling off accidentally.

During the experiment, certain number of culture chambers with different specifications are selected according to the required cell number for plating, the cell immunofluorescence experiment can be directly carried out after the plating is carried out for 24 hours, and finally, the cells can be observed by a confocal microscope after being sealed by corresponding cover glass.

The utility model discloses a method for using culture plate is as follows: during the experiment, certain number of culture chambers with different specifications are selected according to the required cell number for plating, the cell immunofluorescence experiment is directly carried out after the plating is carried out for 24 hours, and the cells are directly observed by a confocal microscope after the culture chambers are sealed by corresponding cover glass; meanwhile, the culture chamber 7 is arranged on the culture plate support 1 in a detachable structure, so that the culture chamber can be replaced or detached at will according to actual needs, and the convenience of experiments is improved.

The above embodiments are only used for illustrating the technical solution of the present invention, and not for limiting the same; although the present invention has been described in detail with reference to the foregoing embodiments, it will be understood by those skilled in the art; the technical solutions described in the foregoing embodiments may still be modified, or some technical features may be equivalently replaced; such modifications and substitutions do not depart from the spirit and scope of the present invention.

Claims (5)

1. A cell culture plate for cellular immunofluorescence comprises a culture plate support (1), culture chambers (7), cover slips (13) and a culture plate cover (15), wherein the culture plate support (1) comprises a frame (2) and a support frame (3), the frame (2) is arranged on the periphery of the support frame (3), the support frame (3) comprises a transverse plate (4) and a plurality of vertical supports (5), the vertical supports (5) are arranged at the bottom end of the transverse plate (4), the plurality of vertical supports (5) are uniformly and crosswise distributed along the transverse plate (4) in the transverse direction and the longitudinal direction, a plurality of small compartments are defined by the transverse plate (4), the vertical supports (5) and the frame (2), circular holes (14) are arranged on the transverse plate (4) of each small compartment, according to the number of the circular holes (14), the support frame (3) comprises three types of 96 holes, 48 holes and 24 holes to adapt to the culture chambers (7) with different models, the culture chamber (7) is a cylindrical chamber and is characterized in that: be provided with epitaxial plate (8) at cultivation cell (7) top edge level, the end of epitaxial plate (8) is provided with down flange (9), be provided with edge draw-in groove (11) that set up with circular port (14) are concentric in horizontal board (4) upper end, lower flange (9) of every cultivation cell (7) are all dismantled and are fixed alone and use on support frame (3) through edge draw-in groove (11), and the bottom of cultivateing cell (7) is high printing opacity slide (12) of handling through polylysine, coverslip (13) are high printing opacity slide, the top of cultivateing cell (7) is located to the lid, cultivation board lid (15) adaptation cultivation board support (1) and the lid is located cultivation board support (1) outside.

2. The cell culture plate for cellular immunofluorescence according to claim 1, wherein: the coverslip (13) includes 96-well, 48-well and 24-well diameter sizes to fit different models of culture chambers.

3. The cell culture plate for cellular immunofluorescence according to claim 1, wherein: the supporting frames (3) corresponding to the 96-hole, 48-hole and 24-hole models of the supporting frame (3) are respectively distributed into 11 in the transverse direction and 7 in the longitudinal direction; 7 in the transverse direction and 5 in the longitudinal direction; 5 pieces in the transverse direction and 3 pieces in the longitudinal direction.

4. The cell culture plate for cellular immunofluorescence according to claim 1, wherein: the culture chamber (7) further comprises a cylindrical side wall (6), the high-light-transmittance glass slide (12) is arranged at the bottom of the side wall (6), the epitaxial plate (8) is arranged at the top of the side wall (6), and the cover glass (13) is attached to the epitaxial plate (8).

5. The cell culture plate for cellular immunofluorescence according to claim 1, wherein: cover glass (13) set up to convex lens structure, and convex lens (10) set up in cover glass (13) lower extreme, and the diameter size of convex lens (10) is the same with the diameter size of cultivateing cell (7) inner space to restriction cover glass (13) that can be fine prevents that cover glass (13) accident from droing.

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