CN216141560U - Filter container and cell culture device - Google Patents
Filter container and cell culture device Download PDFInfo
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- CN216141560U CN216141560U CN202121931676.4U CN202121931676U CN216141560U CN 216141560 U CN216141560 U CN 216141560U CN 202121931676 U CN202121931676 U CN 202121931676U CN 216141560 U CN216141560 U CN 216141560U
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Abstract
The utility model provides a filtering container and a cell culture device, and relates to the technical field of biological cell filtration, wherein the filtering container comprises a container body; the container body comprises a screen frame; the bottom surface of the screen frame is provided with a bottom wall screen, and the side surface of the screen frame is provided with a side wall screen; the screen frame, the bottom wall screen and the side wall screen enclose a containing cavity with an opening on the top surface. The cell culture apparatus includes a filtration vessel. Through this filter container, solved the cell culture in-process that exists among the prior art and need carry out the technical problem of changing liquid many times to the cell, reached and reduced work load, effective save time, reduced the loss in the cell centrifugation process, reduced the beneficial effect of the produced pollution risk of repeated operation simultaneously.
Description
Technical Field
The utility model relates to the technical field of biological cell filtration, in particular to a filtration container and a cell culture device.
Background
The cell filtering container is mainly used for cell culture, cell classification, liquid replacement and the like in a laboratory. According to the pore size, the purpose of harvesting cells, the removal of other impurities and the like, the cell filtration container with the matched caliber is mainly used in a beaker or a 50ml centrifuge tube, and the related cell filtration container is not designed in a cell culture plate (a cell culture dish, a 6-pore plate, a 12-pore plate, a 24-pore plate and the like).
The method comprises the steps of inducing primary bone marrow cells by different methods to obtain required cell types, wherein the cells can generate suspension and adherent cells in the bone marrow cell induction process, culturing the bone marrow cells by using a cell culture plate or a cell culture dish, changing the liquid for many times during the culture process, collecting the cells into a centrifugal tube, centrifuging, and then changing the liquid. In the operation process, the operation is tedious, the time consumption is long, the workload is increased, and meanwhile, the risk of cell pollution exists and the cells are lost.
SUMMERY OF THE UTILITY MODEL
The utility model aims to provide a filter container and a cell culture device, which are used for solving the technical problem that cells need to be changed for multiple times in the cell culture process in the prior art.
In a first aspect, the present invention provides a filtration vessel comprising: a container body;
the container body comprises a screen frame;
the bottom surface of the screen frame is provided with a bottom wall screen, and the side surface of the screen frame is provided with a side wall screen; the screen frame, the bottom wall screen and the side wall screen enclose an accommodating cavity with an opening on the top surface.
Further, the screen frame comprises an upper annular support frame, a lower annular support frame and a plurality of connecting supports fixedly connected between the upper annular support frame and the lower annular support frame;
the bottom wall screen is fixedly connected to the lower annular support frame;
the side wall screen is fixedly enclosed between the upper annular support frame and the lower annular support frame.
Further, the upper annular support frame, the lower annular support frame and the side wall screen are all circular rings;
the bottom wall screen mesh is circular and is matched with the lower annular support frame.
Furthermore, the screen frame, the bottom screen and the side wall screen are all made of polypropylene biological materials;
and/or the aperture of the bottom wall screen and the aperture of the side wall screen are both 5-100 mu m.
Further, a first handle is arranged on the upper annular supporting frame;
the first handle is of a U-shaped plate-shaped structure, and the opening side of the U-shaped plate-shaped structure is vertically connected to the outer wall surface of the upper annular supporting frame.
Furthermore, the container bodies are arranged in a plurality of numbers, and the container bodies are fixedly connected through a connecting plate;
the connecting plate is arranged close to the top surface opening.
Further, a plurality of the container bodies are arranged in a matrix.
Furthermore, two groups of second handles which are oppositely arranged are arranged on the connecting plate;
the second handle is of a U-shaped plate structure, and the opening side of the U-shaped plate structure is vertically connected to the outer wall surface of the connecting plate.
Has the advantages that:
the utility model provides a filtering container, wherein a container body comprises a screen frame; the bottom surface of the screen frame is provided with a bottom wall screen, and the side surface of the screen frame is provided with a side wall screen; the screen frame, the bottom wall screen and the side wall screen enclose an accommodating cavity with an opening on the top surface, and when the container is used specifically, the container body can be placed in a corresponding cell culture plate, namely, suspended cells can be settled at the bottom of the cell culture plate, so that rapid separation can be realized; when the cell liquid changing work is carried out, the container body can be pulled out, and the liquid changing operation can be directly carried out, so that the workload is greatly reduced, the time is effectively saved, the loss in the cell centrifugation process is reduced, the risk of pollution caused by repeated operation is reduced, and the use and popularization are easy.
In a second aspect, the present invention provides a cell culture apparatus comprising: a cell culture plate and a filtration vessel according to any one of the preceding embodiments;
the cell culture plate is provided with an accommodating groove, and the container body is placed in the accommodating groove.
Further, the accommodating grooves are arranged in one or more than one;
when the holding tank is a plurality of, a plurality of the holding tank is the matrix and arranges.
Has the advantages that:
the cell culture device provided by the utility model comprises the filtering container, so that the technical advantages and effects which can be achieved by the cell culture device also comprise the technical advantages and effects which can be achieved by the filtering container, and are not repeated herein.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, and it is obvious that the drawings in the following description are some embodiments of the present invention, and other drawings can be obtained by those skilled in the art without creative efforts.
FIG. 1 is a schematic structural view of a filtration vessel according to an embodiment of the present invention, wherein the sidewall screen is shown in partial configuration;
FIG. 2 is a schematic view showing the structure of a cell culture plate in which the filtration vessel shown in FIG. 1 is placed in a single receiving well;
FIG. 3 is a schematic diagram of the cell culture plate with the filter vessels of FIG. 1 placed in multiple housing chambers;
FIG. 4 is another schematic structural view of a filtration vessel according to an embodiment of the present invention, wherein a plurality of vessel bodies are fixed together by a connecting plate;
FIG. 5 is a schematic view showing the structure of the cell culture plate in which the filtration vessel shown in FIG. 4 is placed in a multi-well.
Icon:
100-a screen frame; 110-an upper ring support; 120-lower annular support frame; 130-a connecting bracket;
200-bottom wall screen;
300-sidewall screen;
400-a first handle;
500-connecting plate;
600-a second handle;
700-cell culture plate; 710-accommodating the tank.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are some, but not all, embodiments of the present invention. The components of embodiments of the present invention generally described and illustrated in the figures herein may be arranged and designed in a wide variety of different configurations.
Thus, the following detailed description of the embodiments of the present invention, presented in the figures, is not intended to limit the scope of the utility model, as claimed, but is merely representative of selected embodiments of the utility model. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
It should be noted that: like reference numbers and letters refer to like items in the following figures, and thus, once an item is defined in one figure, it need not be further defined and explained in subsequent figures.
In the description of the present invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings or orientations or positional relationships that the products of the present invention are conventionally placed in use, and are only for convenience of describing the present invention and simplifying the description, but do not indicate or imply that the device or element to which the description refers must have a specific orientation, be constructed in a specific orientation, and be operated, and thus should not be construed as limiting the present invention. Furthermore, the terms "first," "second," "third," and the like are used solely to distinguish one from another and are not to be construed as indicating or implying relative importance.
Furthermore, the terms "horizontal", "vertical" and the like do not imply that the components are required to be absolutely horizontal or pendant, but rather may be slightly inclined. For example, "horizontal" merely means that the direction is more horizontal than "vertical" and does not mean that the structure must be perfectly horizontal, but may be slightly inclined.
In the description of the present invention, it should also be noted that, unless otherwise explicitly specified or limited, the terms "disposed," "mounted," "connected," and "connected" are to be construed broadly and may, for example, be fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meanings of the above terms in the present invention can be understood in specific cases to those skilled in the art.
Some embodiments of the utility model are described in detail below with reference to the accompanying drawings. The embodiments described below and the features of the embodiments can be combined with each other without conflict.
Referring to fig. 1, the present embodiment provides a filtering container including a container body; the container body includes a screen frame 100; the bottom of the screen frame 100 is provided with a bottom screen 200, and the side of the screen frame 100 is provided with a side screen 300; the screen frame 100, the bottom wall screen 200 and the side wall screen 300 enclose an open-topped containing cavity.
In the filtering container provided in this embodiment, referring to fig. 2 or fig. 3, in specific use, the container body may be placed in the corresponding cell culture plate 700, that is, the suspended cells may be settled at the bottom of the cell culture plate 700, so as to achieve rapid separation; when the cell liquid changing work is carried out, the container body can be pulled out, the liquid changing operation can be directly carried out, so that the workload is greatly reduced, the time is effectively saved, the loss in the cell centrifugation process is reduced, meanwhile, the risk of pollution caused by repeated operation can be reduced, and the cell liquid changing device is easy to use and popularize.
In addition, the filter container has wide application range and can be used for cell culture, cell classification, liquid replacement and the like in a laboratory.
Referring to fig. 1, the screen frame 100 includes an upper ring support 110, a lower ring support 120, and a plurality of connecting brackets 130 fixedly connected therebetween; the bottom screen 200 is fixedly connected to the lower annular support frame 120; the sidewall screen 300 is fixedly enclosed between the upper ring support 110 and the lower ring support 120.
Through the above-mentioned setting to screen frame 100, not only can improve screen frame 100's overall structure's steadiness, moreover, go up annular support frame 110, lower annular support frame 120 and linking bridge 130 three all can regard as the installation carrier for it is fixed to install diapire screen cloth 200 and lateral wall screen cloth 300.
Alternatively, the sidewall screen 300 may be fixedly connected to the connecting bracket 130 by bonding or the like, and the upper edge of the sidewall screen 300 is tightly abutted to the upper annular supporting frame 110, and the lower edge of the sidewall screen 300 is tightly abutted to the lower annular supporting frame 120.
In other embodiments, the upper edge of the sidewall screen 300 may be adhered to the outer wall surface or the inner wall surface of the upper ring support 110, and the lower edge of the sidewall screen 300 may be adhered to the outer wall surface or the inner wall surface of the lower ring support 120.
In this embodiment, referring to fig. 1, the upper annular support frame 110, the lower annular support frame 120, and the sidewall screen 300 are all circular rings; the bottom screen 200 is circular and fits into the lower annular support frame 120.
In other embodiments, the shape of the material may be rectangular or other irregular shapes, and may be reasonably set according to actual needs.
In this embodiment, the screen frame 100, the bottom screen 200 and the side screen 300 are made of polypropylene biomaterial; the filtering container made of the polypropylene biomaterial can be repeatedly sterilized under high pressure and reused, so that the cost can be reduced.
Furthermore, the aperture of the bottom screen 200 and the aperture of the side screen 300 are both 5-100 μm, which can be selected according to the specific cell shape and diameter.
In this embodiment, be equipped with the handle on the filter container, only need during the use handheld handle will filter the container and put into cell culture plate 700, convenient, and reducible pollution.
Specifically, the upper ring-shaped supporting frame 110 is provided with a first handle 400.
Referring to fig. 1, in one embodiment of the present application, the first handle 400 has a "U" -shaped plate structure, and an open side of the "U" -shaped plate structure is vertically connected to an outer wall surface of the upper ring support 110, so that the "U" -shaped plate structure can be held by a hand to transfer the filtering container when in use.
Referring to fig. 4, a plurality of container bodies are provided, and the plurality of container bodies are fixedly connected with each other through a connecting plate 500; the connecting plate 500 is arranged close to the opening of the top surface, namely, the integrated multi-cavity filter container is arranged, so that the filter container can be conveniently lifted and pulled at one time, and the use convenience of an operator is improved.
Referring to fig. 5, with connecting plate 500 disposed close to the top surface opening, on the basis of realizing connection between a plurality of vessel bodies, the presence of connecting plate 500 does not interfere with the lowering of a plurality of vessel bodies in accommodating groove 710 of cell culture plate 700, that is, the vertical distance at which a plurality of vessel bodies are lowered simultaneously can be increased.
Different numbers of filter containers can be designed according to the size and depth of the accommodating groove 710 of the cell culture plate 700 corresponding to the use, such as a single-accommodating-cavity filter container, a six-accommodating-cavity filter container, a twelve-accommodating-cavity filter container, and the like.
Further, the plurality of container bodies are arranged in a matrix.
Illustratively, the plurality of container bodies are arranged in a matrix of two rows and three columns; of course, the matrix arrangement is not limited to two rows and three columns, and may be three rows and three columns, for example.
Referring to fig. 4, two sets of second handles 600 are disposed on the connecting plate 500; the second handle 600 is of a U-shaped plate structure, and the open side of the U-shaped plate structure is vertically connected to the connecting plate 500 or the outer wall surface of the upper ring-shaped support frame 110, so that the second handle 600 can be supported by both hands to move when in use.
Referring to fig. 2, 3, or 5, the present embodiment further provides a cell culture apparatus including a cell culture plate 700 and the aforementioned filter container; the cell culture plate 700 is provided with an accommodating groove 710, and the container body is placed in the accommodating groove 710.
Specifically, the accommodating grooves 710 are provided in one piece, and as shown in FIG. 2, the cell culture plate 700 is provided with one accommodating groove 710 for accommodating one container body.
Referring to fig. 3 or 5, when there are a plurality of receiving grooves 710, the plurality of receiving grooves 710 are arranged in a matrix, and one container body may be placed on the multi-receiving-groove cell culture plate (see fig. 3 in particular), or a plurality of container bodies of the same number as the number of receiving grooves may be placed on the multi-receiving-groove cell culture plate (see fig. 5 in particular).
Illustratively, six, twelve, twenty-four receiving wells 710 may be provided on cell culture plate 700.
It should be noted that, since the filtering container needs to be placed in the accommodating groove 710, the height of the filtering container is reasonably set according to the depth of the accommodating groove 710 of different cell culture plates 700 or according to the requirement of the experiment, so as to ensure that the height of the filtering container can be higher than the depth of the accommodating groove 710, thereby facilitating the taking out of the filtering container.
Finally, it should be noted that: the above embodiments are only used to illustrate the technical solution of the present invention, and not to limit the same; while the utility model has been described in detail and with reference to the foregoing embodiments, it will be understood by those skilled in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some or all of the technical features may be equivalently replaced; and the modifications or the substitutions do not make the essence of the corresponding technical solutions depart from the scope of the technical solutions of the embodiments of the present invention.
Claims (10)
1. A filtration vessel, comprising: a container body;
the container body comprises a screen frame (100);
a bottom wall screen (200) is arranged on the bottom surface of the screen frame (100), and a side wall screen (300) is arranged on the side surface of the screen frame (100); the screen frame (100), the bottom wall screen (200) and the side wall screen (300) enclose an accommodating cavity with an open top surface.
2. The filtration vessel of claim 1, wherein the screen frame (100) comprises an upper annular support frame (110), a lower annular support frame (120), and a plurality of connecting brackets (130) fixedly connected therebetween;
the bottom wall screen (200) is fixedly connected to the lower annular support frame (120);
the side wall screen (300) is fixedly enclosed between the upper annular support frame (110) and the lower annular support frame (120).
3. The filtration vessel of claim 2, wherein the upper annular support frame (110), the lower annular support frame (120), and the sidewall screen (300) are each circular;
the bottom wall screen (200) is in a circular shape matched with the lower annular support frame (120).
4. The filtration vessel of claim 1, wherein the screen frame (100), the bottom screen (200) and the side wall screen (300) are all made of polypropylene biomaterial;
and/or the aperture of the bottom wall screen (200) and the aperture of the side wall screen (300) are both 5-100 mu m.
5. A filtering container according to claim 2, characterized in that said upper annular support (110) is provided with a first handle (400);
the first handle (400) is of a U-shaped plate structure, and the opening side of the U-shaped plate structure is vertically connected to the outer wall surface of the upper annular support frame (110).
6. The filtering container as claimed in any one of claims 2 to 4, wherein the container body is provided in plurality, and the container bodies are fixedly connected with each other through a connecting plate (500);
the connecting plate (500) is disposed proximate to the top surface opening.
7. The filtration vessel of claim 6, wherein a plurality of the vessel bodies are arranged in a matrix.
8. A filtering container according to claim 6, characterized in that said connecting plate (500) is provided with two sets of second handles (600) arranged opposite each other;
the second handle (600) is of a U-shaped plate structure, and the opening side of the U-shaped plate structure is vertically connected to the outer wall surface of the connecting plate (500).
9. A cell culture apparatus, comprising: a cell culture plate (700) and a filtration vessel according to any one of claims 1 to 8;
an accommodating groove (710) is formed in the cell culture plate (700), and the container body is placed in the accommodating groove (710).
10. The cell culture apparatus according to claim 9, wherein the holding tank (710) is provided in one or more;
when the accommodating groove (710) is plural, the plural accommodating grooves (710) are arranged in a matrix.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202121931676.4U CN216141560U (en) | 2021-08-17 | 2021-08-17 | Filter container and cell culture device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202121931676.4U CN216141560U (en) | 2021-08-17 | 2021-08-17 | Filter container and cell culture device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN216141560U true CN216141560U (en) | 2022-03-29 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202121931676.4U Active CN216141560U (en) | 2021-08-17 | 2021-08-17 | Filter container and cell culture device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN216141560U (en) |
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2021
- 2021-08-17 CN CN202121931676.4U patent/CN216141560U/en active Active
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Address after: 215000 station 6-013, 6 / F, No. 88, modern Avenue, Suzhou Industrial Park, Suzhou area, China (Jiangsu) pilot Free Trade Zone, Suzhou, Jiangsu (cluster registration) Patentee after: Tiankang Pharmaceutical Co.,Ltd. Address before: 215000 station 6-013, 6 / F, No. 88, modern Avenue, Suzhou Industrial Park, Suzhou area, China (Jiangsu) pilot Free Trade Zone, Suzhou, Jiangsu (cluster registration) Patentee before: Tiankang Pharmaceutical (Suzhou) Co.,Ltd. |