CN211964612U - Centrifugal device for cell centrifugation and preparation - Google Patents
Centrifugal device for cell centrifugation and preparation Download PDFInfo
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- CN211964612U CN211964612U CN201921948667.9U CN201921948667U CN211964612U CN 211964612 U CN211964612 U CN 211964612U CN 201921948667 U CN201921948667 U CN 201921948667U CN 211964612 U CN211964612 U CN 211964612U
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Abstract
The utility model relates to a centrifugal device for cell centrifugation and preparation, which comprises a centrifugal cup and a centrifugal cup holder, wherein the upper layer of the centrifugal cup holder is a limiting plate, the lower layer of the centrifugal cup holder is a bracket base, the bracket base and the limiting plate are connected through four height-adjustable pillars, and a plurality of centrifugal cup fixing clamping grooves are uniformly distributed on the upper surface of the limiting plate and used for clamping the centrifugal cup; the centrifugal cup comprises a centrifugal cup body, a centrifugal cover and centrifugal cup lugs, the centrifugal cover is installed on the upper portion of the centrifugal cup body in a threaded mode, the middle of the centrifugal cup body is a cylindrical cup body, the bottom of the centrifugal cup body is integrally made into a reverse conical cup bottom, a chamfer is further manufactured at the bottom end of the reverse conical cup bottom, the two centrifugal cup lugs are symmetrically and fixedly installed on the circumferential wall of the upper portion of the centrifugal cup body, and scales and identification areas are further arranged on the circumferential wall of the middle of the centrifugal cup body. The utility model discloses reduce the experimental time, improve experimental efficiency, avoid the reagent pollution that experimental operation caused repeatedly, greatly reduced experimental space takes the convenience simultaneously.
Description
Technical Field
The utility model belongs to the technical field of experiment auxiliary assembly, a device for experiments and supplementary fixing device are used to bulky cell collection and supernatant collection is related to, especially a centrifugal device for cell centrifugation, preparation.
Background
With the development of society, the popularization of information and the promotion of the biomedical industry, stem cells are known to the public more and more, and more research institutions of researchers participate in the stem cell industry. Because the stem cells are a type of early undifferentiated pluripotent cells with self-replication renewal and multiple differentiation, the research on the stem cells is continuously developed, the stem cell technology is gradually improved and perfected, and various stem cells such as hematopoietic stem cells, umbilical cord mesenchymal stem cells, adipose mesenchymal stem cells and the like gradually enter the research category of laboratories.
In recent years, due to changes of environment and living habits of people, the incidence rate of tumors is higher and higher, certain side effects exist in radiotherapy, chemotherapy and surgical treatment, cellular immunotherapy is continuously taken as a new auxiliary treatment means and becomes a focus of attention and research of people in various fields, the immunotherapy can be said to be the best, and particularly in the field of cancer treatment, the immunotherapy is an unprecedented breakthrough. The immune cells of human body are divided into a plurality of types, and the immune cell therapy of tumors also comprises different types, mainly comprising non-specific old technologies represented by CIK, DC-CIK, NK and the like and specific new technologies represented by CAR-T, TCR-T and needing in vitro gene modification.
Regardless of any cell research method, the research of cells is both the starting point of life science and the convergence point of life science, and the emerging point is the cell culture technology, the cell collection work in cell culture, when the cell culture demand is large, the collection workload of the supernatant and the collection workload of the cells are huge, the consumption of experimental consumables and the time consumption cost are high, the collection device used in the laboratory at present mainly comprises centrifuge tubes, when the collection of the supernatant or the preparation of cell preparations are carried out in large batch, a plurality of centrifuge tubes are needed to be used at the same time, one sub-package and one another are combined again, a plurality of centrifuge tubes need to be placed by a plurality of centrifuge tube racks at the same time, the mixing is easy, the pollution is easy, the experimental space is occupied, the manpower and material resources are wasted, the time consumption is long, influence on the cell state and are not beneficial for the experiment operator to grasp the amount of the supernatant and the cell preparation as a whole.
No patent documents relevant to the present application were found by search.
SUMMERY OF THE UTILITY MODEL
An object of the utility model is to overcome prior art's weak point, provide one kind and be convenient for experiment operation, can reduce the experimental time, improve experimental efficiency, avoid the reagent pollution that experiment operation caused repeatedly, greatly reduced experimental space occupancy takes convenient a centrifugal device for cell centrifugation, preparation moreover.
The utility model provides a its technical problem take following technical scheme to realize:
a centrifugal device for cell centrifugation and preparation comprises a centrifugal cup and a centrifugal cup holder, wherein the centrifugal cup holder is of a two-layer frame structure, the upper layer of the centrifugal cup holder is a limiting plate, the lower layer of the centrifugal cup holder is a support base, four corners of the upper surface of the support base are respectively provided with a height-adjustable strut, the upper ends of the four height-adjustable struts are jointly provided with the limiting plate, the upper surface of the limiting plate is uniformly provided with a plurality of centrifugal cup fixing clamping grooves, and the centrifugal cup is coaxially clamped in the centrifugal cup fixing clamping grooves; the centrifugal cup comprises a centrifugal cup body, a centrifugal cover and centrifugal cup lugs, the upper portion of the centrifugal cup body is a cup opening, the centrifugal cover is installed at the position of the cup opening in a threaded mode, the middle of the centrifugal cup body is a cylindrical cup body, an inverted cone-shaped cup bottom is integrally formed at the bottom of the centrifugal cup body, a chamfer is further manufactured at the bottom end of the inverted cone-shaped cup bottom, two centrifugal cup lugs are symmetrically and fixedly installed on the circumferential wall of the upper portion of the centrifugal cup body, and scales and identification areas are further arranged on the circumferential wall of the middle cylindrical cup body section of the centrifugal cup.
And the height of the centrifugal cup holder is the same as the distance from the bottom of the centrifugal cup to the centrifugal cup ear.
And, folding upper portion handle and lateral part handle still symmetry system are made to centrifugal glass stand both sides.
Moreover, the radius length of the chamfer is set to be 30mm, and the arc length is 60 mm.
Moreover, the centrifuge cup is made of medical grade polypropylene materials.
And four suckers are fixedly arranged at four corners of the lower surface of the bracket base of the centrifugal rack cup holder.
The utility model has the advantages that:
1. this device can be used for the collection of cell supernatant and cell preparation in-process cell suspension, also can be used for holding and centrifugation of large capacity reagent in the laboratory, the utility model discloses a centrifugation cup can hold the cell suspension about 200mL, need not divide according to 50mL centrifuging tube partial shipment like, practice thrift the experimental time, and supporting centrifugation glass stand, can fix the centrifugation cup, with the neat and orderly arrangement of centrifugation cup, practice thrift experiment operating space, the angle can set up the inclination of centrifugation cup rim of a cup according to the experiment needs by adjusting nut is placed to the centrifugation cup simultaneously, and the mass supernatant or cell suspension are once only collected in batches, avoid causing reagent to pollute because of multiple operation, the utility model provides a centrifugation cup is centrifuged in can arranging centrifuge in corresponding centrifuge bowl in, need not be equipped with other centrifugation auxiliary assembly, practices thrift the experiment consumptive material.
2. The utility model discloses a centrifugation cup, the collection of mainly used bulk reagent can once only collect a large amount of cell supernatants or cell suspensions, has avoided the collection cell that relapse, can save time, avoids the cell to lose, guarantees the collection volume of cell, guarantees simultaneously that the cell is in good state.
3. The utility model discloses a cup ear of centrifugation cup upper portion setting, this cup ear mainly make things convenient for the experimenter to get and put the operation, but does not influence centrifugal operation, and traditional centrifuging tube structure as an organic whole after liquid remains or the experimenter is with the clean gloves of alcohol, takes place the roll-off accident very easily for the cell sediment that the bottle bottom was collected after the centrifugation goes to liquid in the suspension again, needs carry out the secondary centrifugation.
4. The centrifugal cup is made of medical grade polypropylene materials, is uniform in sterilization and packaging, can be directly used, and reduces early preparation work of experiments; the utility model discloses a bottleneck of centrifugation cup is wider than traditional centrifuging tube, makes things convenient for experiment operator to operate.
5. The centrifugal cup holder designed in the utility model can be used for placing a plurality of centrifugal cups at one time, thereby saving experimental space and avoiding collision between the centrifugal cups and affecting the quality of collected sediment; in this device, the pillar of centrifugal glass stand is adjustable, can be according to experiment operation needs, and adjusting nut adjusts suitable angle, the convenient experiment operation with centrifugal rim of a cup.
Drawings
FIG. 1 is a schematic structural view of the present invention;
FIG. 2 is a schematic view of a centrifuge cup;
FIG. 3 is a schematic view of a centrifuge cup holder configuration;
fig. 4 is a usage state diagram of the present invention.
Detailed Description
The present invention will be described in further detail with reference to the following embodiments, which are illustrative, not restrictive, and the scope of the invention should not be limited thereto.
The utility model provides a centrifugal device for cell centrifugation, preparation, includes centrifugal cup 1 and centrifugal cup frame 2, centrifugal cup frame is a two-layer frame construction, centrifugal cup frame upper strata is limiting plate 5, and the lower floor is support base 7, but height-adjusting's pillar 6 is all installed in support base upper surface four corners, the limiting plate is installed jointly to four height-adjusting's pillar upper ends, limiting plate upper surface equipartition is arranged and is had a plurality of centrifugal cup fixed slot 17, the coaxial centrifugal cup that has clamped in the centrifugal cup fixed slot.
The centrifugal cup comprises a centrifugal cup body 13, a centrifugal cover 12 and centrifugal cup lugs 14, wherein the upper part of the centrifugal cup body is a cup opening, the centrifugal cover is installed at the cup opening in a threaded mode, the middle of the centrifugal cup body is a cylindrical cup body 15, the bottom of the centrifugal cup body is integrally manufactured into an inverted cone-shaped cup bottom 10, in order to be capable of better matching with a centrifugal groove structure of the centrifugal cup, a chamfer 9 is manufactured at the bottom end of the inverted cone-shaped cup bottom, the radius length of the chamfer is set to be 30mm, the arc length is 60mm, two centrifugal cup lugs are symmetrically and fixedly installed on the circumferential wall of the upper part of the centrifugal cup body, the depth of a centrifugal groove of the centrifugal cup is the same as the distance from the cup bottom of the centrifugal cup to the centrifugal cup lugs, and in order; the centrifugal cup is made of transparent medical grade polypropylene, and the circumferential wall of the middle cylindrical cup body section of the centrifugal cup is also provided with scales 11 and a mark area 16.
The height of centrifugal glass stand is the same with the distance from the bottom of the centrifugal glass to the ear of the centrifugal glass, and for the convenience of transferring the centrifugal glass stand, the two sides of the centrifugal glass stand are also symmetrically provided with an upper handle 3 and a lateral handle 4, and the upper handle and the lateral handle can be folded, so that the experimental space can be saved when the centrifugal glass stand is not used.
Centrifugal cup holder is when using, but centrifugal cup holder's height-adjusting's pillar is heightened through adjusting nut 19 for centrifugal cup on the centrifugal cup holder is the slope form, makes things convenient for experiment operator to carry out the experiment operation.
Four suckers 8 are fixedly arranged at four corners of the lower surface of the bracket base of the centrifugal rack cup holder, so that the placing stability is greatly improved.
The utility model discloses in the implementation, not receive the friction of centrifugal cup draw-in groove and impaired in order to make the lateral wall of centrifugal cup the centrifugal cup draw-in groove inner wall all pastes and covers has wear-resisting layer 18.
During the use, in the centrifugation cup is once only collected to the supernatant or the cell suspension that will collect, cover supporting centrifugal lid, observe the scale mark, should not exceed the 20mL volume of scale mark, after collecting several bottles, put into the centrifuge and carry out the centrifugation, place the centrifugation cup in the centrifugation cup fixed slot on the centrifugation cup holder after the centrifugation, then with centrifuge cup holder immigration to the operation panel in, handheld centrifugation cup ear, pour the supernatant, put back in centrifuge cup fixed slot, adjusting nut, make the centrifugation cup place suitable inclination in, carry out experimental operation on next step.
The utility model combines the defects of the existing experimental device, provides a large-capacity centrifugal cup which is more convenient for experimental operation, saves operation space and improves experimental efficiency and a supporting bracket thereof, the device can be used for collecting cell suspension in the process of cell supernatant and cell preparation and can also be used for containing and centrifuging large-capacity reagent in a laboratory, the centrifugal cup designed by the utility model can contain about 200mL of cell suspension without being subpackaged according to 50mL of centrifugal tube, the experimental time is saved, the supporting bracket can fix the centrifugal cup, the centrifugal cup is orderly arranged, the experimental operation space is saved, simultaneously, the placing angle of the centrifugal cup can be adjusted by a nut according to the experimental needs, and large-batch supernatant or cell suspension can be collected at one time, the reagent pollution caused by multiple operations is avoided, the centrifugal cup in the utility model can be placed in a corresponding centrifugal groove of a centrifugal machine for centrifugation, need not be equipped with other centrifugation auxiliary assembly, practice thrift the experiment consumptive material.
The centrifugal cup designed by the utility model is mainly used for collecting large-dose reagents, is 5 times of the collection amount of the traditional centrifugal tube, can collect a large amount of cell supernatant or cell suspension at one time, avoids repeated cell collection, can save time, avoids cell loss, ensures the collection amount of cells, and simultaneously ensures that the cells are in a good state;
the utility model discloses a cup ear of centrifugation cup upper portion setting, this cup ear mainly make things convenient for the experimenter to get and put the operation, but does not influence centrifugal operation, and traditional centrifuging tube structure as an organic whole after liquid remains or the experimenter is with the clean gloves of alcohol, takes place the roll-off accident very easily for the cell sediment that the bottle bottom was collected after the centrifugation goes to liquid in the suspension again, needs carry out the secondary centrifugation.
The centrifugal cup is made of medical grade polypropylene materials, is uniform in sterilization and packaging, can be directly used, and reduces early preparation work of experiments;
the bottle mouth of the centrifugal cup designed by the utility model is wider than the traditional centrifugal tube, which is convenient for the experiment operator to operate;
the centrifugal cup holder designed in the utility model can be used for placing a plurality of centrifugal cups at one time, thereby saving experimental space and avoiding collision between the centrifugal cups and affecting the quality of collected sediment;
in this device, the pillar of centrifugal cup frame has one side to design for can adjusting, can be according to experiment operation needs, adjusting nut adjusts suitable angle, makes things convenient for experiment operation with centrifugal rim of a cup.
Although the embodiments of the present invention and the accompanying drawings are disclosed for illustrative purposes, those skilled in the art will appreciate that: various substitutions, changes and modifications are possible without departing from the spirit and scope of the present invention and the appended claims, and therefore, the scope of the present invention is not limited to the disclosure of the embodiments and the accompanying drawings.
Claims (6)
1. A centrifugation device for centrifugation of cells and preparations, characterized in that: the centrifugal cup holder is of a two-layer frame structure, the upper layer of the centrifugal cup holder is a limiting plate, the lower layer of the centrifugal cup holder is a support base, four corners of the upper surface of the support base are provided with height-adjustable pillars, the limiting plates are jointly arranged at the upper ends of the four height-adjustable pillars, a plurality of centrifugal cup fixing clamping grooves are uniformly distributed and arranged on the upper surface of each limiting plate, and centrifugal cups are coaxially clamped in the centrifugal cup fixing clamping grooves; the centrifugal cup comprises a centrifugal cup body, a centrifugal cover and centrifugal cup lugs, the upper portion of the centrifugal cup body is a cup opening, the centrifugal cover is installed at the position of the cup opening in a threaded mode, the middle of the centrifugal cup body is a cylindrical cup body, an inverted cone-shaped cup bottom is integrally formed at the bottom of the centrifugal cup body, a chamfer is further manufactured at the bottom end of the inverted cone-shaped cup bottom, two centrifugal cup lugs are symmetrically and fixedly installed on the circumferential wall of the upper portion of the centrifugal cup body, and scales and identification areas are further arranged on the circumferential wall of the middle cylindrical cup body section of the centrifugal cup.
2. A centrifugation device for cell centrifugation, preparations, according to claim 1, characterized in that: the height of the centrifugal cup holder is the same as the distance from the bottom of the centrifugal cup to the centrifugal cup ear.
3. A centrifugation device for cell centrifugation, preparations, according to claim 1, characterized in that: folding upper handles and side handles are symmetrically arranged on two sides of the centrifugal cup holder.
4. A centrifugation device for cell centrifugation, preparations, according to claim 1, characterized in that: the radius length of the chamfer is set to be 30mm, and the arc length is 60 mm.
5. A centrifugation device for cell centrifugation, preparations, according to claim 1, characterized in that: the centrifugal cup is made of medical grade polypropylene materials.
6. A centrifugation device for cell centrifugation, preparations, according to claim 1, characterized in that: four suckers are fixedly arranged at four corners of the lower surface of the bracket base of the centrifugal cup holder.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201921948667.9U CN211964612U (en) | 2019-11-13 | 2019-11-13 | Centrifugal device for cell centrifugation and preparation |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201921948667.9U CN211964612U (en) | 2019-11-13 | 2019-11-13 | Centrifugal device for cell centrifugation and preparation |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN211964612U true CN211964612U (en) | 2020-11-20 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201921948667.9U Active CN211964612U (en) | 2019-11-13 | 2019-11-13 | Centrifugal device for cell centrifugation and preparation |
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| Country | Link |
|---|---|
| CN (1) | CN211964612U (en) |
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- 2019-11-13 CN CN201921948667.9U patent/CN211964612U/en active Active
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