CN214735684U - Mechanical actuating mechanism of PCR diagnostic apparatus - Google Patents

Abstract

The utility model relates to an experimental facilities technical field, concretely relates to PCR diagnostic apparatus mechanical actuator, the on-line screen storage device comprises a base, the base upper end is equipped with the casing, be equipped with the heating pipe of vortex form in the casing, the heating pipe upper end is equipped with the opening and upwards just can place the reaction chamber of heating fluid, axial fixed axle about the reaction intracavity is equipped with between two parties, axial and the ascending section of thick bamboo of placing of opening about the fixed axle upper end is equipped with, place a section of thick bamboo lower extreme and set up a plurality of intercommunication mouths that communicate with the reaction chamber, axial refrigerating pipe about four end angular position in the reaction chamber are equipped with respectively, the casing upper end is equipped with and can shelters from reaction chamber open-ended apron, this simple structure, convenient operation, the practicality is strong, the utility model discloses there is extensive application prospect in experimental facilities technical field.

Description

Mechanical actuating mechanism of PCR diagnostic apparatus

Technical Field

The utility model relates to an experimental facilities technical field, concretely relates to PCR diagnostic apparatus mechanical actuator.

Background

The PCR technology is an in vitro or in vitro synthesis experiment of a specific gene by using DNA polymerase, and is a specific linkage replication method by using DNA polymerase, so that people can purposefully amplify specific nucleic acid fragments in vitro by using the existing PCR technology, and the principle is similar to the in vivo replication of DNA. Through the development of many years, the technology is mature and is the most important and commonly used technology in the fields of molecular biology research and clinical diagnosis, and the PCR instrument is developed to realize automation of the technology, so that the application of the PCR technology is wider.

In the temperature-variable PCR reaction process, the extraction and amplification of nucleic acid and the temperature cycling conditions required by reaction entities are key steps for determining the accuracy of the nucleic acid detection result. In the traditional temperature-variable PCR reaction process, an operator performs a nucleic acid extraction step, transfers an extracted nucleic acid reagent to an amplification test tube, and then performs temperature cycling on the amplification test tube by using a thermal cycling system, wherein the temperature cycling comprises high-temperature denaturation, low-temperature annealing and medium-temperature primer extension.

The current PCR instrument is not sensitive enough to control the temperature, when a sample tube is heated, the heating temperature is not uniform, the copying and detecting speed of genes can be influenced, the experimental result is not accurate, the gene reaction is not thorough, and the like, so that a mechanical actuating mechanism of the PCR diagnostic instrument is urgently needed, and the problem that the reaction test tube of the existing PCR instrument is not uniform in heating is solved.

SUMMERY OF THE UTILITY MODEL

To the above-mentioned condition, sensitive inadequately to the control of temperature for overcoming current PCR appearance, when heating the sample cell, heating temperature is heated inhomogeneous can influence the speed of gene's duplication, detection, leads to the unsafe problem of experimental result, the utility model aims at providing a PCR diagnostic apparatus mechanical actuator, the effectual current PCR appearance reaction test tube of having solved is heated inhomogeneous problem.

The technical scheme of its solution is, the utility model discloses a base, the base upper end is equipped with the casing, be equipped with the heating pipe of vortex form in the casing, the heating pipe upper end is equipped with the opening and upwards just can place the reaction chamber of heating liquid, axial fixed axle about the reaction intracavity is equipped with between two parties, axial and the ascending section of thick bamboo of placing of opening about the fixed axle upper end is equipped with, place a section of thick bamboo lower extreme and set up a plurality of intercommunication mouths with the reaction chamber intercommunication, four end angle positions in the reaction chamber are equipped with axial refrigerating pipe from top to bottom respectively, the casing upper end is equipped with and shelters from reaction chamber open-ended apron.

Preferably, the upper end of the shell is provided with a connecting column which is in left-right axial direction and is hinged with the cover plate.

Preferably, the front end of casing is equipped with the control screen, the upper end of every refrigeration pipe is electric connection respectively has the connecting wire, every connecting wire all electric connection of each other and its terminal and control screen electric connection.

Preferably, the free end of the heating pipe is electrically connected with the control screen through a connecting wire.

Preferably, a plurality of support legs in the up-down direction are respectively arranged on the periphery of the lower end of the base.

Compared with the prior art, the beneficial effects of the utility model are that: (1) the reaction tube is heated fully and the heating temperature is more uniform during reaction by heating the heating liquid, so that the reaction of samples in the reaction tube is facilitated, and the accuracy of a sample result is facilitated; (2) through the refrigerating tube, the connecting wire and the control screen, when the temperature is too high, the refrigerating tube can be started through the control screen, so that the temperature is rapidly reduced, the temperature of a reaction test tube in the reaction cavity is conveniently controlled, and the requirement of a PCR instrument on the temperature is favorably met; (3) the cover plate can completely shield the upper end opening of the reaction cavity, so that the temperature in the reaction cavity can not be rapidly lost, an experimental result is influenced, and the sealing property and the heat preservation property of the device can be improved; (4) the operation and use of experimenters are facilitated through the base, the support legs and the shell; this simple structure, convenient operation, the practicality is strong, the utility model discloses there is extensive application prospect in experimental facilities technical field.

Drawings

Fig. 1 is an isometric view of the present invention.

Fig. 2 is a full-section right perspective view diagram of the utility model.

Fig. 3 is a full-section top perspective view of the utility model.

Fig. 4 is an enlarged view of a in fig. 2 according to the present invention.

Fig. 5 is an enlarged view of B in fig. 3 according to the present invention.

The device comprises a base 1, a base 2, a shell 3, a heating pipe 4, a reaction cavity 5, a fixing shaft 6, a placing cylinder 7, a communication port 8, a refrigerating pipe 9, a cover plate 10, a connecting column 11, a control screen 12, a connecting line 13 and supporting legs.

Detailed Description

The technical solutions of the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings, and it is to be understood that the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative efforts belong to the protection scope of the present invention, and in the description of the present invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc. indicate the orientation or position relationship based on the orientation or position relationship shown in the drawings, and are only for convenience of description of the present invention and simplifying the description, but do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus cannot be understood as limiting the present invention.

Furthermore, the terms "first," "second," and "third" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance throughout the present disclosure, it being understood that unless otherwise expressly specified or limited, the terms "mounted," "connected," and "connected" are to be construed broadly and can be, for example, fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meaning of the above terms in the present invention can be understood in specific cases to those skilled in the art.

As shown in figures 1-5, a PCR diagnostic apparatus mechanical actuator, including the base 1, the base 1 is a cube, the base 1 is round-cornered, which is favorable to bearing the mechanism, the material is bakelite, it is light and high temperature resistant, it is convenient to make the base 1 heated when the heating tube 3 is heated, the upper end of the base 1 is equipped with the shell 2, the base 1 and the shell 2 are fixedly connected, and the connecting surface between the base 1 and the shell 2 is sealed by sealant, which ensures the air tightness of the device, is favorable to the experiment, the shell 2 is internally equipped with the vortex-shaped heating tube 3 in the middle, the heating tube 3 is a resistance wire, it can heat the reaction chamber 4, the reaction chamber 4 with an upward opening and capable of placing the heating liquid is arranged above the heating tube 3, the reaction chamber 4 is made of alloy aluminum material, which is favorable to absorb the heat of the heating tube 3, and is convenient to heat the heating liquid rapidly, heating liquid sets up in reaction chamber 4, can heat the heating liquid in reaction chamber 4 through heating pipe 3, through heating liquid, can make the reaction test tube be heated more evenly, is favorable to the reaction of sample in the reaction test tube.

As shown in the figure 1-5, a PCR diagnostic apparatus mechanical actuator, a reaction chamber 4 in the center of the fixed connection with the vertical axis of the fixed shaft 5, convenient to place the tube 6 for fixing, the fixed shaft 5 upper end fixed connection with the vertical axis and upward opening of the placing tube 6, placing 6 in the tube for placing the reaction tube, placing 6 in the tube is provided with a fixed clamp can fix the reaction tube, can make the reaction tube centralized in the tube 6, is convenient for the operator to perform the experiment operation and observe the experiment results, placing 6 lower end open with a plurality of tube 7 connected with the reaction chamber 4, connecting opening 7 for four arc through holes and placed in the tube 6 lower end, the reaction chamber 4 in the heating liquid level is greater than the reaction tube in the sample position height, is convenient for the heating liquid to flow into the tube 6 quickly, is favorable to the reaction tube heating, four end angle positions of the reaction cavity 4 are respectively and fixedly connected with an upper axial refrigerating pipe 8 and a lower axial refrigerating pipe 8, so that the temperature of the reaction test tube during the experiment can be conveniently controlled.

As shown in figures 1-5 a PCR diagnostic apparatus mechanical actuator, the upper end of the housing 2 is equipped with a cover plate 9 which can cover the opening of the reaction chamber 4, specifically, the rear end of the housing 2 is fixedly connected with a left and right axial connecting column 10 through a support frame, the rear end of the cover plate 9 is equipped with a left and right axial jack which can be matched with the connecting column 10, during installation, the cover plate 9 is hinged with the housing 2 through the connecting column 10, the cover plate 9 is turned upwards, the cover plate 9 swings upwards, at the moment, a reaction test tube can be placed into the placing cylinder 6, the cover plate 9 is pushed downwards, the opening of the housing 2 can be covered by the cover plate 9, the cover plate 9 is arranged to be slightly larger than the opening at the upper end of the housing 2, which is convenient for sealing and heat preservation of the apparatus and improving the air tightness of the apparatus, when the apparatus is used, the cover plate 9 can be provided with a buckle, the housing 2 is provided with a snap ring which can be buckled with the buckle, through further fixing the cover plate 9, the stability of the device is improved.

As shown in fig. 1-5, a mechanical actuator of a PCR diagnostic apparatus, the front end of a casing 2 is provided with a square control screen 11, the front end of the casing 2 is provided with a notch with the same shape as the control screen 11, when the PCR diagnostic apparatus is installed and used, the control screen 11 is placed in the notch to fix the control screen 11 conveniently, the upper end of each cooling tube 8 is electrically connected with a connecting wire 12, each connecting wire 12 is electrically connected with each other, the terminal of the connecting wire is electrically connected with the control screen 11, the cooling tube 8 can be started for cooling or stopped for cooling through the control screen 11, the free end of a heating tube 3 is electrically connected with the control screen 11 through the connecting wire 12, the heating tube 3 can be started for heating or stopped for heating through the control screen 11 to control the temperature in a reaction chamber 4 conveniently, the periphery of the lower end of a base 1 is fixedly connected with up-down support legs 13, the device is convenient to fix and is beneficial to the experiment of operators.

When the device is used, firstly, the cover plate 9 is rotated upwards, the reaction test tube is placed in the placing barrel 6, heating liquid is injected into the reaction cavity 4, when the heating liquid is slightly higher than the height position of a sample in the reaction test tube, the injection of the heating liquid into the reaction cavity 4 is stopped, the cover plate 9 is rotated downwards, the cover plate 9 completely shields the upper end opening of the shell 2, the control screen 11 is started, a heating program is started, at the moment, the heating pipe 3 starts to heat the heating liquid, the heating liquid transmits heat to the reaction test tube in the placing barrel 6, at the moment, the reaction test tube starts to react, if the reaction test tube is cooled, the heating program is closed, the refrigerating program of the control screen 11 is opened, the refrigerating pipe 8 starts to refrigerate the heating liquid, at the moment, the cooling operation of the temperature of the reaction test tube can be finished, after the sample in the reaction test tube is reacted, the cover plate 9 is rotated upwards, the reaction tube was removed.

The above-mentioned embodiments only represent some embodiments of the present invention, and the description thereof is specific and detailed, but not to be construed as limiting the scope of the present invention. It should be noted that, for those skilled in the art, without departing from the spirit of the present invention, several variations and modifications can be made, which are within the scope of the present invention. Therefore, the protection scope of the present invention should be subject to the appended claims.

Claims (5)

1. The mechanical actuating mechanism of the PCR diagnostic instrument comprises a base (1) and is characterized in that a shell (2) is arranged at the upper end of the base (1), a vortex-shaped heating pipe (3) is arranged in the shell (2), a reaction cavity (4) with an opening capable of being upwards placed is arranged at the upper end of the heating pipe (3), an upper axial fixing shaft (5) and a lower axial fixing shaft (5) are arranged in the reaction cavity (4) in the middle, an upper placing cylinder (6) with an upper axial direction and an upper opening capable of being upwards placed is arranged at the upper end of the fixing shaft (5), a plurality of communicating ports (7) communicated with the reaction cavity (4) are formed in the lower end of the placing cylinder (6), an upper axial refrigerating pipe (8) and a lower axial refrigerating pipe (8) are respectively arranged at four end corner positions of the reaction cavity (4), and a cover plate (9) capable of shielding the opening of the reaction cavity (4) is arranged at the upper end of the shell (2).
2. 2. The mechanical actuator of the PCR diagnostic apparatus according to claim 1, wherein the upper end of the housing (2) is provided with a connecting column (10) which is axially arranged at left and right sides and is hinged with the cover plate (9).
3. 3. The mechanical actuator of the PCR diagnostic apparatus according to claim 1, wherein the front end of the housing (2) is provided with a control screen (11), the upper end of each cooling tube (8) is electrically connected with a connecting wire (12), each connecting wire (12) is electrically connected with each other, and the terminal of each connecting wire is electrically connected with the control screen (11).
4. 4. The mechanical actuator of the PCR diagnostic apparatus according to claim 1, wherein the free end of the heating tube (3) is electrically connected to the control screen (11) via a connecting wire (12).
5. 5. The mechanical actuator of the PCR diagnostic apparatus according to claim 1, wherein the periphery of the lower end of the base (1) is provided with a plurality of support legs (13) in the vertical direction.

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