CN216919241U

CN216919241U - Novel PCR reaction tube for molecular diagnosis experiment

Info

Publication number: CN216919241U
Application number: CN202220310525.5U
Authority: CN
Inventors: 秦雷
Original assignee: Xuzhou Haichuan Biological Research Institute Co ltd
Current assignee: Xuzhou Haichuan Biological Research Institute Co ltd
Priority date: 2022-02-16
Filing date: 2022-02-16
Publication date: 2022-07-08
Anticipated expiration: 2032-02-16

Abstract

The utility model discloses a novel PCR reaction tube for molecular diagnosis experiments, which comprises a tube body, wherein an external thread is arranged on the outer wall of the tube body close to the upper end, a tube cap is rotatably connected to the upper end surface of the tube body, an internal thread is arranged on the inner wall of the tube cap close to the lower end, a sliding groove is arranged on the side wall of the tube cap, a plurality of inner through holes are arranged on the inner wall of the tube cap, a plurality of outer through holes are arranged on the outer wall of the tube cap at positions corresponding to the inner through holes, a top cover is arranged on the upper end surface of the tube cap, a plurality of baffles are fixedly connected to the edge of the lower end surface of the top cover, the baffles are matched and slide on the side wall of the sliding groove, and a limit groove is arranged on the inner wall of the sliding groove close to the lower end. Through the use to the device, can heat up or cool down the gene book that awaits measuring fast, improve the efficiency of experiment to the device makes in the experimentation become more convenient to the inside medicine that adds of body, has saved manufacturing cost simultaneously.

Description

Novel PCR reaction tube for molecular diagnosis experiment

Technical Field

The utility model relates to the field of PCR reaction tubes, in particular to a novel PCR reaction tube for molecular diagnosis experiments.

Background

Molecular diagnostics refers to techniques for making a diagnosis by detecting changes in the structure or expression level of genetic material in a patient using molecular biology methods. Molecular diagnosis is the main method for prediction diagnosis, and can be used for diagnosis of individual genetic diseases and prenatal diagnosis. Molecular diagnostics mainly refers to the detection of genes encoding various structural proteins, enzymes, antigens, antibodies, and immunologically active molecules associated with diseases.

The prior patent (publication number is CN203128569U) discloses a novel PCR reaction tube, and particularly relates to the technical field of medical detection articles. It contains toper container pipe (1), reaction tube pipe cap connecting piece (2), reaction tube pipe cap (4), and it still includes high-efficient heat transfer layer (3), one-way seal membrane (5) its characterized in that, the upper end of toper container pipe (1) connect reaction tube pipe cap (4) through reaction tube pipe cap connecting piece (2), its characterized in that toper container pipe (1) in the middle of be provided with high-efficient heat transfer layer (3), reaction tube pipe cap (4) inside be provided with one-way seal membrane (5). Its simple structure increases in the middle of traditional PCR reaction tube has high-efficient heat-conducting layer, can heat up rapidly and lower the temperature and improve reaction efficiency, and the inside one-way membrane accessible probe that is provided with of tube cap is direct to inside increase medicine, convenient and fast, easy operation.

However, this device still has some problems: the device has set up high-efficient heat transfer layer in the pipe shaft lateral wall of reaction tube inside, and the PCR reaction tube is a disposable experimental equipment nevertheless, installs high-efficient heat transfer layer additional to the reaction tube and has mentioned heat conduction efficiency, but also improved manufacturing cost and the production technology degree of difficulty simultaneously greatly, very inconvenient to the one-way seal membrane that the device set up on the pipe cap can only use to visit intraductal increase medicine, and it is longer to add the medicine time, and efficiency is lower.

SUMMERY OF THE UTILITY MODEL

The utility model aims to solve the defects in the prior art and provides a novel PCR reaction tube for molecular diagnosis experiments.

In order to achieve the purpose, the utility model adopts the following technical scheme: a novel PCR reaction tube for molecular diagnosis experiment comprises a tube body, wherein an external thread is arranged on the outer wall of the upper end of the tube body, a tube cap is rotatably connected on the upper end surface of the tube body, an internal thread is arranged on the inner wall of the lower end of the tube cap, a sliding chute is arranged on the side wall of the tube cap, a plurality of internal through holes are arranged on the inner wall of the tube cap, a plurality of external through holes are arranged at the positions corresponding to the internal through holes on the outer wall of the tube cap, a top cover is arranged on the upper end surface of the tube cap, a plurality of baffles are fixedly connected at the edge of the lower end surface of the top cover, the baffles slide on the side wall of the sliding chute in a matching manner, a limiting groove is arranged on the inner wall of the lower end of the sliding chute, a limiting block is fixedly connected at the lower end of the inner wall of the baffle, the limiting block slides on the inner wall of the limiting groove in a matching manner, and a stop block is fixedly connected at the middle end of the inner wall of one side of the baffle, the upper end face of the pipe cap is provided with a first feeding hole, and the upper end face of the top cover is provided with a second feeding hole.

As a further description of the above technical solution:

the pipe body and the pipe cap are connected together through the external thread and the internal thread.

As a further description of the above technical solution:

the side wall of the stop block is contacted with the side wall of the inner through hole.

As a further description of the above technical solution:

the pipe body, the pipe cap and the top cover are all made of polypropylene.

As a further description of the above technical solution:

the inner through hole and the outer through hole are the same in size.

As a further description of the above technical solution:

the baffle is larger in size than the inner through hole.

As a further description of the above technical solution:

the first feed inlet and the second feed inlet are the same in size.

As a further description of the above technical solution:

and a shifting block is fixedly connected to one side of the upper end surface of the top cover.

The utility model has the following beneficial effects:

in the utility model, the device is through using the mode of circulating hot-blast and circulating cold wind to the gene sample that awaits measuring of reaction tube inside to go up and down the temperature, through setting up interior through-hole and outer through-hole messenger hot-blast or cold wind directly with the gene sample contact that awaits measuring to the efficiency of the intensification and the cooling of improvement to the gene sample that awaits measuring improves the quality of experiment.

In the utility model, the device makes the top cap rotate through stirring the shifting block, and the top cap drives the baffle and rotates, and when the lateral wall of dog touched on the lateral wall of interior through-hole, made the baffle rotate to between interior through-hole and the outer through-hole, just in time got up interior through-hole and outer through-hole are sealed, and first feed inlet and second feed inlet intercommunication can conveniently add the reaction medicine to the pipe shaft simultaneously.

Drawings

FIG. 1 is a perspective view of a novel PCR reaction tube for molecular diagnostic experiments according to the present invention;

FIG. 2 is a cross-sectional view of a novel PCR reaction tube for molecular diagnostic experiments according to the present invention;

FIG. 3 is a schematic diagram of a top cap of a novel PCR reaction tube for molecular diagnostic experiments according to the present invention;

FIG. 4 is a schematic view of the tube body of the novel PCR reaction tube for molecular diagnostic experiments according to the present invention.

Illustration of the drawings:

1. a tube body; 2. an external thread; 3. a pipe cap; 4. an internal thread; 5. a chute; 6. an inner through hole; 7. an outer through hole; 8. a top cover; 9. a baffle plate; 10. a limiting groove; 11. a limiting block; 12. a stopper; 13. a first feed port; 14. a second feed port; 15. and (5) shifting blocks.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be obtained by a person skilled in the art without making any creative effort based on the embodiments in the present invention, belong to the protection scope of the present invention.

In the description of the present invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc. indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and simplification of description, but do not indicate or imply that the device or element referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus, should not be construed as limiting the present invention; the terms "first," "second," and "third" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance, and furthermore, unless otherwise explicitly stated or limited, the terms "mounted," "connected," and "connected" are to be construed broadly and may be, for example, fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meanings of the above terms in the present invention can be understood in specific cases to those skilled in the art.

Referring to fig. 1-4, one embodiment of the present invention is provided: the utility model provides a novel PCR reaction tube for molecular diagnosis experiment, includes pipe shaft 1, pipe shaft 1 lean on to be provided with external screw thread 2 on the upper end outer wall, rotates on pipe shaft 1's the up end and is connected with pipe cap 3, pipe cap 3 lean on to be provided with internal thread 4 on the lower extreme inner wall, pipe shaft 1 and pipe cap 3 through external screw thread 2 with internal thread 4 threaded connection together, can directly rotate pipe cap 3 after the reaction is accomplished and take off, directly pour the gene sample of 1 inside of pipe shaft, it is very convenient.

Be provided with spout 5 on the lateral wall of pipe cap 3, be provided with a plurality of interior through-holes 6 on the inner wall of pipe cap 3, be provided with a plurality of outer through-holes 7 for interior through-hole 6 position department on the outer wall of pipe cap 3, be provided with top cap 8 on the up end of pipe cap 3, a plurality of baffles 9 of the lower terminal surface edge fixedly connected with of top cap 8, baffle 9 cooperation slides on the lateral wall of spout 5, interior through-hole 6 is the same with the size of outer through-hole 7, baffle 9's size is compared in interior through-hole 6 great, baffle 9 can block interior through-hole 6 and outer through-hole 7 sealedly.

Chute 5 leans on being provided with spacing groove 10 on the lower extreme inner wall, baffle 9's inner wall leans on lower extreme department fixedly connected with stopper 11, stopper 11 cooperation slides on spacing groove 10's inner wall, one side inner wall middle-end fixedly connected with dog 12 of baffle 9, the lateral wall of dog 12 and the lateral wall contact of interior through-hole 6, be provided with first feed inlet 13 on the up end of pipe cap 3, be provided with second feed inlet 14 on the up end of top cap 8, first feed inlet 13 is the same with second feed inlet 14's size, can make things convenient for the injection of reaction medicine, top cap 8's up end one side department fixedly connected with shifting block 15, make top cap 8 rotate on the up end of pipe cap 3 through stirring shifting block 15, pipe shaft 1 is the polypropylene with pipe cap 3 and top cap 8's material.

The working principle is as follows: when the device is used, a gene sample to be detected is injected into the tube body 1, then the tube cap 3 is rotatably connected to the tube body 1 through the external thread 2 at the upper end of the tube body 1 and the internal thread 4 at the lower end of the tube cap 3, the shifting block 15 is shifted to enable the baffle plate 9 to rotate, when one side wall of the baffle plate 12 is contacted with one side wall of the internal through hole 6, the internal through hole 6 is communicated with the external through hole 7, so that circulating hot air or circulating cold air can directly heat or cool the gene sample to be detected in the tube body 1, the experimental efficiency is improved, at the moment, the first feed port 13 is sealed and shielded by the top cover 8, when a medicine needs to be added into the tube body 1 in an experiment, the shifting block 15 can be reversely shifted, the baffle plate 9 reversely rotates, when the other side wall of the baffle plate 12 is contacted with the other side wall of the internal through hole 6, the baffle plate 9 seals and blocks the internal through hole 6 and the external through hole 7, and the second feed port 14 is communicated with the first feed port 13 due to rotary displacement, can conveniently add the medicine to 1 inside of pipe shaft to through-hole 6 is sealed with outer through-hole 7 in when adding the medicine, and the medicine flows out pipe shaft 1 because of the setting of interior through-hole 6 and outer through-hole 7 and causes the waste, only needs behind the medicine completion to stir shifting block 15 forward once more and just can make interior through-hole 6 and outer through-hole 7 intercommunication, continues to experiment, and is very convenient.

Finally, it should be noted that: although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that modifications may be made to the embodiments or portions thereof without departing from the spirit and scope of the utility model.

Claims

1. A novel PCR reaction tube for molecular diagnostic experiments, which comprises a tube body (1), and is characterized in that: the pipe comprises a pipe body (1), wherein an external thread (2) is arranged on the outer wall of the upper end of the pipe body (1), a pipe cap (3) is rotatably connected onto the upper end face of the pipe body (1), an internal thread (4) is arranged on the inner wall of the lower end of the pipe cap (3), a sliding groove (5) is arranged on the side wall of the pipe cap (3), a plurality of inner through holes (6) are arranged on the inner wall of the pipe cap (3), a plurality of outer through holes (7) are arranged on the outer wall of the pipe cap (3) relative to the inner through holes (6), a top cover (8) is arranged on the upper end face of the pipe cap (3), a plurality of baffles (9) are fixedly connected onto the edge of the lower end face of the top cover (8), the baffles (9) are matched and slide on the side wall of the sliding groove (5), a limiting groove (10) is arranged on the inner wall of the lower end of the sliding groove (5), and a limiting block (11) is fixedly connected onto the inner wall of the baffle (9) close to the lower end, stopper (11) cooperation is slided on the inner wall of spacing groove (10), one side inner wall middle-end fixedly connected with dog (12) of baffle (9), be provided with first feed inlet (13) on the up end of pipe cap (3), be provided with second feed inlet (14) on the up end of top cap (8).

2. The novel PCR reaction tube for molecular diagnostic experiments according to claim 1, characterized in that: the pipe body (1) and the pipe cap (3) are connected together through the external thread (2) and the internal thread (4) in a threaded manner.

3. The novel PCR reaction tube for molecular diagnostic experiments according to claim 1, characterized in that: the side wall of the stop block (12) is contacted with the side wall of the inner through hole (6).

4. The novel PCR reaction tube for molecular diagnostic experiments according to claim 1, characterized in that: the pipe body (1), the pipe cap (3) and the top cover (8) are all made of polypropylene.

5. The novel PCR reaction tube for molecular diagnostic experiments according to claim 1, characterized in that: the inner through hole (6) and the outer through hole (7) are the same in size.

6. The novel PCR reaction tube for molecular diagnostic experiments according to claim 5, wherein: the baffle (9) is larger than the inner through hole (6).

7. The novel PCR reaction tube for molecular diagnostic experiments according to claim 1, characterized in that: the first feed opening (13) and the second feed opening (14) are the same in size.

8. The novel PCR reaction tube for molecular diagnostic experiments according to claim 1, characterized in that: and a shifting block (15) is fixedly connected to one side of the upper end surface of the top cover (8).