CN214703661U

CN214703661U - Protein tracing instrument

Info

Publication number: CN214703661U
Application number: CN202120749153.1U
Authority: CN
Inventors: 蔡福营
Original assignee: Guangzhou Wanlinwei Biotechnology Co ltd
Current assignee: Guangzhou Wanlinwei Biotechnology Co ltd
Priority date: 2021-04-13
Filing date: 2021-04-13
Publication date: 2021-11-12
Anticipated expiration: 2031-04-13

Abstract

The utility model discloses a protein tracing appearance relates to western blot appearance technical field, including L shape base, the mounting groove has been seted up at the top of L shape base, slidable mounting has disconnect-type gel box in the mounting groove, one side difference fixed mounting of disconnect-type gel box has fixed cover and spacing, slidable mounting has the slider in the fixed cover, the top of slider is rotated and is installed round bar and fixed mounting has the limiting plate. The utility model discloses in, utilize the electrophoresis utmost point to lead into positive and negative electrode respectively, carry out the electrophoresis shaping to the sample in the disconnect-type gel box, after the electrophoresis takes shape, it drives the round bar and makes injection head and discharge orifice comb realize the position conversion to rotate the cylinder piece this moment, utilize the discharge orifice comb to sample afterwards, the sample of being extracted carries out antibody reaction through placing the upper surface at the reaction plate and detects, at this moment, promote the push pedal and make the reaction plate remove, rock at the in-process that removes, realize accelerating antibody reaction speed, improve sampling efficiency.

Description

Protein tracing instrument

Technical Field

The utility model relates to a western blot appearance technical field, in particular to western blot appearance.

Background

The protein blotting instrument is a stable instrument specially designed and produced for the operation of a detection reagent of the protein immunoblotting method, and the product is suitable for institutions or units for carrying out the research and detection of the protein immunoblotting method, such as universities, scientific research institutions, disease control centers, clinical laboratory departments, central laboratories and the like.

Present protein trace appearance function singleness, the flexibility ratio is not enough, and the operation is more loaded down with trivial details when changing the system ware in the sample process, wastes time and energy, uses comparatively inconveniently, and antibody reaction rate is slower, and then leads to whole consuming time longer, influences work efficiency, for this reason, this application has provided a protein trace appearance.

SUMMERY OF THE UTILITY MODEL

The application aims to provide a protein blotter to solve the problems in the background technology.

In order to achieve the above purpose, the present application provides the following technical solutions: the utility model provides a protein tracing instrument, includes L shape base, the mounting groove has been seted up at the top of L shape base, slidable mounting has disconnect-type gel box in the mounting groove, one side difference fixed mounting of disconnect-type gel box has fixed cover and spacing, slidable mounting has the slider in the fixed cover, the top of slider is rotated and is installed round bar and fixed mounting has the limiting plate, the top fixed mounting of round bar has the mounting panel, the through-hole has been seted up on the top side of mounting panel, the top side fixed mounting of mounting panel has the filling opening, the both ends bottom side fixed mounting of mounting panel has a pair of hollow plate, and a pair of bottom side difference fixed mounting of hollow plate has a plurality of injection heads and a plurality of discharge hole comb, the bottom of filling opening runs through in proper order through the through-hole, one of them hollow plate and a plurality of the injection head are linked together, one side fixed mounting of L shape base has the toper tray, the bottom of the conical tray is provided with a liquid guide port, one side of the conical tray is provided with a mounting hole, a moving plate is slidably mounted in the mounting hole, the top side of the moving plate is fixedly provided with a vibrating plate and a pair of positioning blocks respectively, and the pair of positioning blocks are detachably provided with the same reaction plate.

Preferably, the mounting groove is close to the equal fixed mounting in both sides inner wall and has a support, two the both sides that the support kept away from each other are fixed mounting respectively has the electrophoresis utmost point.

Preferably, guide grooves are formed in the sides, away from each other, of the separated gel boxes, the two supports correspond to the two guide grooves in position, and the two supports are located in the two guide grooves respectively.

Preferably, a cylindrical part is rotatably mounted on the peripheral side of the round rod, and the cylindrical part and the limiting plate are located on the same horizontal plane.

Preferably, a push plate is fixedly mounted at one end of the moving plate, and a pair of springs is fixedly connected to one side, close to the tapered tray, of the push plate.

Preferably, the reaction plate is located directly above the vibration plate.

Preferably, a receiving box is slidably mounted on the top side of the L-shaped base, a handle is fixedly mounted on one side of the receiving box, and the receiving box is located right below the tapered tray.

To sum up, the utility model discloses a technological effect and advantage:

1. the utility model discloses in, utilize the electrophoresis utmost point to lead into positive negative electrode respectively, carry out the electrophoresis shaping to the sample in the disconnect-type gel box, after the electrophoresis shaping, rotate cylinder spare this moment and drive the round bar and make injection head and discharge orifice comb realize the position conversion, utilize discharge orifice comb to take a sample afterwards, the sample of being extracted carries out antibody reaction through placing the upper surface at the reaction plate and detects, at this moment, promote the push pedal and make the reaction plate remove, rock in the in-process that removes, realize accelerating antibody reaction speed, improve sampling efficiency;

2. the utility model discloses in, through the setting of disconnect-type gel box, can pour the gel of different concentrations in proper order respectively according to the detection demand, and then can conveniently take a sample to the sample of different levels, set up through the cooperation of cylinder spare and limiting plate, can play the limiting action to the angle, can be convenient for pour into head and discharge orifice comb and realize the position conversion, simplify the operation process, the flexibility ratio when simple structure and effectual increasement are used.

Drawings

In order to more clearly illustrate the embodiments of the present application or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present application, and other drawings can be obtained by those skilled in the art without creative efforts.

Fig. 1 is a schematic perspective view of a protein blotting instrument in an embodiment of the present application;

FIG. 2 is another schematic perspective view of a protein blotter in an embodiment of the present application;

FIG. 3 is an enlarged perspective view of the L-shaped base;

fig. 4 is an enlarged perspective view of the split gel cassette.

In the figure: 1. an L-shaped base; 2. an electrophoresis electrode; 3. a split gel box; 4. a guide groove; 5. fixing a sleeve; 6. a slider; 7. a round bar; 8. a cylindrical member; 9. mounting a plate; 10. a hollow slab; 11. an injection port; 12. an injection head; 13. a flow hole comb; 14. a conical tray; 15. pushing the plate; 16. moving the plate; 17. a spring; 18. a reaction plate; 19. a vibrating plate; 20. a limiting strip; 21. a receiving box; 22. and (5) positioning the blocks.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.

Example (b): referring to fig. 1-4, a protein track plotter, which comprises an L-shaped base 1, wherein the top of the L-shaped base 1 is provided with a mounting groove, a separated gel box 3 is slidably mounted in the mounting groove, one side of the separated gel box 3 is respectively and fixedly provided with a fixing sleeve 5 and a limiting strip 20, the fixing sleeve 5 is slidably mounted with a sliding block 6, the top of the sliding block 6 is rotatably provided with a round rod 7 and a limiting plate, the top end of the round rod 7 is fixedly provided with a mounting plate 9, the top side of the mounting plate 9 is provided with a through hole, the top side of the mounting plate 9 is fixedly provided with an injection port 11, the bottom sides of the two ends of the mounting plate 9 are fixedly provided with a pair of hollow plates 10, the bottom sides of the pair of hollow plates 10 are respectively and fixedly provided with a plurality of injection heads 12 and a plurality of combing holes 13, the bottom of the injection port 11 sequentially penetrates through the through hole, one hollow plate 10 is communicated with the plurality of injection heads 12, one side of the L-shaped base 1 is fixedly provided with a conical tray 14, the bottom of the conical tray 14 is provided with a liquid guide port, one side of the conical tray 14 is provided with a mounting hole, a moving plate 16 is slidably mounted in the mounting hole, the top side of the moving plate 16 is fixedly provided with a vibrating plate 19 and a pair of positioning blocks 22 respectively, and the pair of positioning blocks 22 are detachably provided with the same reaction plate 18. By providing the moving plate 16, the vibrating plate 19, the positioning block 22, and the reaction plate 18 can be moved, and the sample on the upper surface of the reaction plate 18 can be shaken.

As a preferred embodiment of this embodiment, the mounting grooves are fixedly mounted with brackets on the inner walls of two sides close to each other, and the two sides far away from each other of the two brackets are respectively fixedly mounted with the electrophoresis electrodes 2. Through the setting of electrophoresis utmost point 2, can be convenient for insert positive and negative electrode, can carry out the electrophoresis to the sample and take shape.

As a preferred embodiment of this embodiment, the separated gel boxes 3 are provided with guide grooves 4 on the sides away from each other, two supports correspond to the two guide grooves 4, and the two supports are respectively located in the two guide grooves 4. Through the corresponding setting in position of two supports and two guide ways 4, can optimize and occupy the proportion and be listed as, can play certain guard action to electrophoresis pole 2 simultaneously, extension equipment life.

As a preferable embodiment of the present embodiment, a cylindrical member 8 is rotatably mounted on the circumferential side of the round bar 7, and the cylindrical member 8 and the limit plate are located on the same horizontal plane. Through the cooperation setting of cylindrical member 8 with the limiting plate, can play and play the limited action to the turned angle of round bar 7.

As a preferred embodiment of this embodiment, a push plate 15 is fixedly mounted at one end of the moving plate 16, and a pair of springs 17 are fixedly connected to the side of the push plate 15 close to the tapered tray 14. Through the cooperation setting of push pedal 15 and spring 17, can make movable plate 16 produce the round trip movement, produce at the in-process that removes and rock, can realize effectual clamp splice antibody reaction rate this moment.

In a preferred embodiment of the present embodiment, the reaction plate 18 is located directly above the vibrating plate 19. Through the setting of vibration board 19, vibration board 19 can carry out the round trip to slide in the toper tray 14, can effectually guarantee to rock the stationarity of in-process.

As a preferred embodiment of the present embodiment, a receiving box 21 is slidably mounted on the top side of the L-shaped base 1, a handle is fixedly mounted on one side of the receiving box 21, and the receiving box 21 is located right below the tapered tray 14. By the corresponding arrangement of the receiving box 21 and the tapered tray 14, the antibody liquid to be detected can automatically fall into the receiving box 21 through the liquid guide port, so that the antibody liquid can be conveniently and intensively processed in the next step.

This practical theory of operation:

firstly, a protein sample is led in through an injection port 11, the led protein sample sequentially enters the injection port 11 through a through hole and a hollow plate 10 and flows into a separation type gel box 3, meanwhile, gel with different concentrations can be led in sequentially by the separation type gel box 3, after the gel and the protein sample are filled, a positive electrode and a negative electrode are respectively led in by an electrophoresis electrode 2, at the moment, the sample in the separation type gel box 3 can be subjected to electrophoresis forming, after the electrophoresis forming, a cylindrical member 8 can be rotated to drive a round rod 7 to rotate, further, the injection head 12 and a flow hole comb 13 can realize position conversion, at the moment, a limiting plate limits the cylindrical member 8, at the moment, the sample can be sampled by the flow hole comb 13, the extracted sample is placed on the upper surface of a reaction plate 18 to carry out antibody reaction detection, at the moment, a push plate 15 is pushed to drive a moving plate 16 to move a vibrating plate 19 and the reaction plate 18, under spring 17's effect, can make it produce round trip movement, produce at the in-process that removes and rock, can realize effectual clamp splice antibody reaction rate this moment, the antibody fluid after being detected can fall into automatically through leading the liquid mouth and receive box 21 in, can effectual improvement sampling efficiency.

Through the setting of disconnect-type gel box 3, can pour the gel of different concentrations in proper order respectively according to the detection demand, and then can conveniently take a sample to the sample of different levels, set up through the cooperation of cylinder 8 and limiting plate, can play the limited action to the angle, can be convenient for pour into head 12 and discharge orifice comb 13 and realize the position conversion, simplify the operation process, the flexibility ratio when simple structure and effectual increasement are used.

Finally, it should be noted that: although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that modifications and variations can be made in the embodiments or in part of the technical features of the embodiments without departing from the spirit and the scope of the invention.

Claims

1. The utility model provides a protein trace appearance, includes L shape base (1), its characterized in that: the mounting groove has been seted up at the top of L shape base (1), slidable mounting has disconnect-type gel box (3) in the mounting groove, one side difference fixed mounting of disconnect-type gel box (3) has fixed cover (5) and spacing (20), slidable mounting has slider (6) in fixed cover (5), the top of slider (6) is rotated and is installed round bar (7) and fixed mounting has the limiting plate, the top fixed mounting of round bar (7) has mounting panel (9), the through-hole has been seted up on the top side of mounting panel (9), the top side fixed mounting of mounting panel (9) has injection opening (11), the both ends bottom side fixed mounting of mounting panel (9) has a pair of hollow board (10), and is a pair of the bottom side fixed mounting of hollow board (10) has a plurality of injection heads (12) and a plurality of injection hole combs (13) respectively, the bottom of through-hole (11) runs through in proper order, One of them hollow core plate (10) and a plurality of injection head (12) are linked together, one side fixed mounting of L shape base (1) has toper tray (14), the drain mouth has been seted up to the bottom of toper tray (14), the mounting hole has been seted up to one side of toper tray (14), slidable mounting has movable plate (16) in the mounting hole, the top side of movable plate (16) is fixed mounting respectively has vibration board (19) and a pair of locating piece (22), and is a pair of demountable installation has same reaction plate (18) on locating piece (22).

2. The protein blotter of claim 1, wherein: equal fixed mounting has the support on the both sides inner wall that the mounting groove is close to each other, two the both sides that the support was kept away from each other are fixed mounting respectively has electrophoresis pole (2).

3. The protein blotter of claim 1, wherein: guide grooves (4) are formed in one side, away from each other, of the separated gel box (3), the two supports correspond to the two guide grooves (4), and the two supports are located in the two guide grooves (4) respectively.

4. The protein blotter of claim 1, wherein: the side of the round rod (7) is provided with a cylindrical part (8) in a rotating mode, and the cylindrical part (8) and the limiting plate are located on the same horizontal plane.

5. The protein blotter of claim 1, wherein: one end of the moving plate (16) is fixedly provided with a push plate (15), and one side of the push plate (15) close to the conical tray (14) is fixedly connected with a pair of springs (17).

6. The protein blotter of claim 1, wherein: the reaction plate (18) is located directly above the vibration plate (19).

7. The protein blotter of claim 1, wherein: the top side of the L-shaped base (1) is provided with a receiving box (21) in a sliding mode, one side of the receiving box (21) is fixedly provided with a handle, and the receiving box (21) is located right below the conical tray (14).