CN216386528U

CN216386528U - Acetate fiber film rapid sample application assistor

Info

Publication number: CN216386528U
Application number: CN202122784336.XU
Authority: CN
Inventors: 唐超
Original assignee: Qingdao Agricultural University
Current assignee: Qingdao Agricultural University
Priority date: 2021-11-12
Filing date: 2021-11-12
Publication date: 2022-04-26
Anticipated expiration: 2031-11-12

Abstract

The utility model discloses a rapid sample application assistor for cellulose acetate membranes, which comprises a top plate and a bottom plate, wherein a plurality of membrane clamping grooves with upper and lower openings are formed in the top plate, scales in the upper and lower directions are arranged on one side of each membrane clamping groove, a straight plate can be arranged on the top plate in a front-back sliding mode, and the top plate is arranged on the bottom plate. Compared with the prior art, the utility model does not need to line in advance before electrophoresis, and the sample application position is accurately determined by matching the slidable straight plate with the scale. The film clamping groove can fix the film, and the ruler can be used as a barrier above to ensure that the film cannot move. In addition, the filter paper does not need to be taken alone to absorb the electrode buffer solution, multiple layers of filter paper can be clamped between the top plate and the bottom plate, and the function of absorbing the electrode buffer solution can be automatically realized as long as the acetate fiber film is placed in the corresponding clamping groove. Generally, for experiments of a plurality of samples or experiments of students, the operation is more convenient and faster under the condition of ensuring that the experimental process is correct.

Description

Acetate fiber film rapid sample application assistor

Technical Field

The utility model relates to an acetate fiber film rapid sample application assistor.

Background

In the prior art, the cellulose acetate film must be pre-soaked before electrophoresis, air in a foam-like structure formed by acetyl cellulose is discharged, and meanwhile, an electrode buffer solution is filled, and then positive and negative ions can be electrophoresed under the action of an electric field to form zones. The acetate fiber film products are different, the presoaking time is different, generally not less than 20 minutes, otherwise, the soaking is incomplete, and the electrophoresis effect is influenced. However, the cellulose acetate film is removed from the electrode buffer, and the surface moisture is blotted by filter paper, and the process to spotting must be completed quickly because the cellulose acetate film is gradually dried within 1-2 minutes once it leaves the electrode buffer, and subsequent electrophoresis cannot be performed.

The existing step of spotting the acetate fiber film by electrophoresis delays a long time, and particularly, a student or a person unskilled in operation can easily perform spotting, the acetate fiber film is dried, the previous pre-soaking and spotting work is ineffective, and the experiment process is delayed.

Meanwhile, the acetate fiber film is required to be scribed with straight lines in advance, and even a person skilled in operation does not necessarily have to sample the sample at each time and is parallel to the bottom edge, so that the sample is not skewed; the sample application distance is not fixed, so that the comparison and analysis among different samples are influenced; and the force is easily too large when the film is scribed, so that a very fragile film is scribed into a groove or even is scratched.

The sample of spotting is generally serum, protein liquid, interstitial fluid and other viscous substances, and if not fixed, the cellulose acetate film is likely to be adhered during spotting, so that the spotting part is blurred, and the electrophoretic resolution is reduced.

Therefore, whether the sample application efficiency is improved or not, the sample application time is shortened, and the improvement of the sample application position accuracy is one of the keys of the success of the acetate film electrophoresis. Therefore, a new type of acetate fiber membrane electrophoresis fast sample application assistor is needed in the market to improve the efficiency.

SUMMERY OF THE UTILITY MODEL

The present invention provides a problem in view of the above-mentioned drawbacks of the prior art.

The technical scheme adopted by the utility model for solving the technical problems is as follows: the utility model provides a quick sample application assistor of acetate fiber film, includes roof and bottom plate, set up a plurality of upper and lower open-ended film clamping groove on the roof, the scale of direction about one side of film clamping groove sets up, the slip sets up straight board around can on the roof, the roof sets up on the bottom plate.

Furthermore, the left side and the right side of roof set up the slide rail respectively, the both sides of ruler set up the slider groove respectively, but slider inslot pull sets up flexible slider, set up the spring that struts between flexible slider and the slider groove.

Further, the ruler is a transparent acrylic plate.

Furthermore, the front end of the ruler is provided with a sliding groove connected with the inside of the sliding block groove, and the telescopic sliding block is provided with a sliding column extending out of the sliding groove.

Furthermore, pin holes are respectively formed in the two sides of the top plate close to the edges, positioning pins which correspond to the pin holes one to one are arranged on the bottom plate, and the top plate can be arranged on the bottom plate in a vertically sliding mode.

The utility model has the beneficial effects that:

1. no pre-scribing is required prior to electrophoresis. Scales are arranged on the acetate film electrophoresis rapid sample application assistor, and the sample application position is accurately determined through a slidable straight plate;

2. the acetate film was fixed so as not to be stuck and dislocated by the sample. The sample application assistor is provided with a cellulose acetate film clamping groove, the whole position of the film is fixed firstly, and then a baffle is arranged at the upper part through a sliding ruler at the upper part. Therefore, the acetate fiber film can not move in position in the whole sample application process;

3. the electrode buffer was not aspirated by holding the filter paper alone. After the cellulose acetate film is taken out from the electrode buffer solution, filter paper needs to be found to absorb the surface moisture. The sample application assistor is divided into an upper layer and a lower layer, multiple layers of filter paper can be clamped between the two layers, and the function of absorbing the electrode buffer solution can be automatically realized as long as the acetate fiber film is placed in the corresponding clamping groove.

4. The method can realize the simultaneous sample application of a plurality of acetate fiber films, does not need to repeat the sample application step once every sample application, and is very convenient and fast for the experiment of a plurality of samples or the experiment of students.

Drawings

FIG. 1 is a schematic diagram of the overall structure of the present invention;

FIG. 2 is a top view of the present invention;

FIG. 3 is a cross-sectional view of the present invention;

FIG. 4 is a schematic structural view of the base plate of the present invention;

FIG. 5 is an enlarged view of detail A of FIG. 1;

fig. 6 is an enlarged view of detail B of fig. 3.

In the figure: the device comprises a top plate 1, a thin film clamping groove 11, scales 12, a sliding rail 13, a pin hole 14, a bottom plate 2, a positioning pin 21, a straight scale 3, a slider groove 31, a telescopic slider 32, a sliding column 321, a spreading spring 33 and a sliding groove 34.

Detailed Description

For a better understanding of the present invention, embodiments of the present invention are explained in detail below with reference to fig. 1 to 6.

It should be noted that the directions "front, back, left, right, up and down" described herein are based on the directions "front, back, left, right, up and down" in fig. 1.

The utility model discloses a rapid sample application assistor for an acetate fiber film, which comprises a top plate 1 and a bottom plate 2, wherein the top plate 1 is arranged on the bottom plate 2. The top plate 1 is provided with a plurality of thin film clamping grooves 11 with upper and lower openings, one side of each thin film clamping groove 11 is provided with scales 12 in the upper and lower directions, the scales 12 are in millimeter units (the scales in the drawing are only used for illustration and do not represent the actual size of the utility model), and zero scale lines of the scales are aligned with the bottom edges of the thin film clamping grooves 11 and are used for indicating the sample application distance.

As shown in fig. 1, fig. 3 and fig. 6, the slide rails 13 are respectively disposed on the left side and the right side of the top plate 1, the slide groove 31 is respectively disposed on the left end and the right end of one straight ruler 3, the telescopic slider 32 is drawably disposed in the slide groove 31, and the expanding spring 33 is disposed between the telescopic slider 32 and the slide groove 31. The ruler 3 is slidably arranged in the slide rails 13 of the top plate 1 through the telescopic sliding blocks 32 at the two ends, and the expanding springs 33 in the sliding block grooves 31 release the elastic force of the telescopic sliding blocks 32, so that the telescopic sliding blocks 32 are supported in the slide rails 13, and the ruler 3 is ensured to be stable and not to be moved randomly when not sliding. For convenient observation, the straight edge 3 is a transparent acrylic plate, and the lower part of the straight edge 3 can be seen through the operation.

Considering that the ruler 3 of the acrylic plate can be worn along with the use time (the top plate and the bottom plate can be made of stainless steel or aluminum alloy), the later sliding is not smooth, and the left and right wearing are different, so that the inclination is caused to cause deviation, the ruler 3 is designed to be detachable, and the later-stage replacement is convenient. Specifically, as shown in fig. 6, a sliding groove 34 connected to the inside of the slider groove 31 is provided at the front end of the straightedge 3, and a sliding post 321 extending out of the sliding groove 34 is provided on the telescopic slider 32, so that the telescopic slider 32 can be returned to the slider groove 31 by buckling the sliding post 321 to the inside, thereby removing and replacing the straightedge 3 from the sliding rail 12 of the top plate 1.

In order to ensure that the top plate 1 and the bottom plate 2 are not displaced when the straightedge 3 is pushed, the spotting of the acetate film is affected. As shown in fig. 1 and fig. 4, pin holes 14 are respectively formed in two sides of the top plate 1 near the edges, positioning pins 21 corresponding to the pin holes 13 one by one are arranged on the bottom plate 2, the top plate 1 is slidably arranged on the bottom plate 2 up and down through the matching of the pin holes 14 and the positioning pins 21, and the top plate 1 and the bottom plate 2 are locked through the pin holes 14 and the positioning pins 21, so that the top plate and the bottom plate can synchronously move even if being forced to move, and the sample application process cannot be influenced due to dislocation. The pin holes 14 and the positioning pins 21 are provided with spaces for the filter paper, so that the filter paper is prevented from interference when being clamped in the middle, and at least two filter papers are symmetrically arranged as much as possible.

The using method and the steps of the utility model are as follows:

1. after the acetate fiber film is presoaked, the top plate is taken down from the bottom plate, the filter paper is placed on the bottom plate, multiple layers can be placed as required, then the pin holes of the top plate are aligned to the positioning pins, and then the hands are loosened, so that the top plate clamps the multiple layers of filter paper in the middle through gravity.

2. And taking out the acetate fiber films from the electrode buffer solution in sequence, placing the acetate fiber films with the matte surfaces facing upwards in the film clamping grooves of the top plate, and enabling the filter paper to automatically absorb the electrode buffer solution.

3. And moving the straight plate along the slide way according to the scale of the thin film clamping groove until the straight ruler moves to the required sample application distance position and stops.

4. At the moment, samples such as serum and the like can be dipped by the cover glass and sequentially spotted at the position of the straight plate, and the cover glass is close to the straight plate during spotting.

5. After the sample application, the top plate is taken from the bottom plate, and a plurality of acetate fiber films are left on the filter paper and then are sequentially placed on the electrophoresis tank.

Claims

1. The utility model provides a quick sample application assistor of acetate fiber film which characterized in that: the film clamping device comprises a top plate and a bottom plate, wherein a plurality of film clamping grooves with upper and lower openings are formed in the top plate, scales in the upper and lower directions are arranged on one sides of the film clamping grooves, a ruler is arranged on the top plate in a front-back sliding mode, and the top plate is arranged on the bottom plate.

2. A rapid spotting aid for cellulose acetate membranes as recited in claim 1 wherein: the left side and the right side of the top plate are respectively provided with a sliding rail, the two sides of the ruler are respectively provided with a sliding block groove, a telescopic sliding block is arranged in the sliding block groove in a pulling mode, and a spreading spring is arranged between the telescopic sliding block and the sliding block groove.

3. A rapid spotting aid for cellulose acetate membranes as recited in claim 1 wherein: the ruler is a transparent acrylic plate.

4. A rapid spotting aid for cellulose acetate membranes as recited in claim 2 wherein: the front end of the ruler is provided with a sliding groove connected with the inside of the sliding block groove, and the telescopic sliding block is provided with a sliding column extending out of the sliding groove.

5. A rapid spotting aid for cellulose acetate membranes as recited in claim 1 wherein: the two sides of the top plate are respectively provided with pin holes near the edges, the bottom plate is provided with positioning pins which are in one-to-one correspondence with the pin holes, and the top plate can be arranged on the bottom plate in a vertically sliding manner.