CN210287315U

CN210287315U - Simple and stable cell scratching device

Info

Publication number: CN210287315U
Application number: CN201921103224.XU
Authority: CN
Inventors: 吴安琪; 张赛; 殷刚
Original assignee: Central South University; Xiangya Hospital of Central South University
Current assignee: Central South University; Xiangya Hospital of Central South University
Priority date: 2019-07-15
Filing date: 2019-07-15
Publication date: 2020-04-10
Anticipated expiration: 2029-07-15

Abstract

The utility model discloses a simple and convenient stable cell mar device, include the mar lid that matches the lid with the cell culture board and connect, the mar is covered and is seted up many and cell culture board on the cell culture hole position one-to-one and along the mar window that mar lid thickness direction runs through the mar lid, every mar window department all is provided with the mar sword through the loose axle, loose axle and mar window sliding fit, the loose axle pass the mar sword and with mar sword normal running fit. Adopt the cell mar device of this structure, ensured the straightness accuracy of mar and the uniformity of mar width, practiced thrift test cost simultaneously for the mar data is more accurate reliable, also is favorable to subsequent observation, analysis of shooing.

Description

Simple and stable cell scratching device

Technical Field

The utility model relates to a biological instrument technical field, concretely relates to simple and convenient stable cell mar device.

Background

In biological assays, scratch healing assays are often used to assess the migratory capacity of tumor cells. The experiment can be completed only by a common cell culture plate, a culture medium and the like, and special consumable reagents are not needed, so that the experiment is economical and practical. After adherent monolayers are usually cultured in six-well plates, scratches are made perpendicular to the plates using a 200 μ L tip, the cells are rinsed with PBS, added to the medium and cultured for a certain period of time before they are photographed for observation. But the scratch made by the manual gun head has difficulty in ensuring the straightness of the scratch and the consistency of the width of the scratch. Although scratch test/wound healing culture inserts are available in the market at present, the cost is high, and the culture inserts are not economical and practical.

SUMMERY OF THE UTILITY MODEL

In view of this, the present invention provides a simple and stable cell scratching device to ensure the linearity of the scratch and the consistency of the width of the scratch, and save the test cost.

The utility model discloses an above-mentioned problem is solved to following technical means: the utility model provides a simple and convenient stable cell mar device, includes and covers the mar lid that connects with the cell culture board matching, the mar is covered and is seted up many and cell culture board on the cell culture hole position one-to-one and along the mar lid thickness direction and run through the mar window that the mar was covered, every mar window department all is provided with the mar sword through the loose axle, loose axle and mar window sliding fit, the loose axle pass the mar sword and with mar sword normal running fit.

Further, the both sides inner wall of every mar window all seted up with the both ends difference sliding fit's of the loose axle that corresponds spout.

Further, every mar window all is parallel with the width direction of mar lid, and the direction that the mar window extends is unanimous with the direction of cell culture hole diameter.

Further, the scratching knife comprises a handheld handle, a connecting handle and a scratching blade which are sequentially connected from top to bottom.

Further, the movable shaft passes through the joint of the handheld handle and the connecting handle, and the total length of the connecting handle and the scratching blade is equal to the vertical length between the movable shaft and the bottom of the cell culture hole.

Further, the width of the tip of the scoring blade is 0.4-0.6 mm.

The utility model has the advantages that: the utility model discloses a simple and convenient stable cell mar device, include and match the mar lid that covers the joint with the cell culture board, the mar cover set up many and cell culture board on the cell culture hole position one-to-one and along the mar window that mar lid thickness direction runs through the mar lid, every mar window department all is provided with the mar sword through the loose axle, loose axle and mar window sliding fit, the loose axle pass the mar sword and with the mar sword normal running fit. Adopt the cell mar device of this structure, ensured the straightness accuracy of mar and the uniformity of mar width, practiced thrift test cost simultaneously for the mar data is more accurate reliable, also is favorable to subsequent observation, analysis of shooing.

Drawings

The invention is further described with reference to the following figures and examples.

Fig. 1 is a schematic top view of the present invention;

fig. 2 is a schematic longitudinal cutting diagram using the present invention.

Wherein: 1-scratch cover, 2-scratch knife, 3-movable shaft, 4-scratch window, 21-handheld handle, 22-connecting handle and 23-scratch blade.

Detailed Description

To facilitate understanding of the present invention, the present invention will be described more fully and specifically with reference to the accompanying drawings and preferred embodiments, but the scope of the present invention is not limited to the specific embodiments described below.

As in fig. 1-2, simple and convenient stable cell mar device, include and cover scratch lid 1 that connects with the cell culture board matching, the scratch is covered and is seted up many and cell culture hole position one-to-one on the cell culture board and along scratch lid thickness direction run through scratch window 4 that the scratch was covered, every scratch window all is parallel with the width direction of scratch lid, the direction that the scratch window extends is unanimous with the direction of cell culture hole diameter, every scratch window department all is provided with mar sword 2 through loose axle 3, when not in use, mar sword horizontal arrangement can hold the mar sword completely through the scratch window, the both ends difference sliding fit's spout with the loose axle that corresponds is all seted up to the both sides inner wall of every scratch window.

The mar sword includes handheld handle 21, connecting handle 22 and the mar blade 23 that top-down connected gradually, the loose axle passes the junction of handheld handle and connecting handle, and loose axle and mar sword normal running fit, the total length of connecting handle and mar blade equals the perpendicular length between loose axle and the cell culture hole bottom to ensure that mar in-process mar blade can contact the cell layer smoothly. The width of the scoring blade is 0.4-0.6mm, preferably 0.5 mm.

When needing to the cell mar, drive the mar sword through handheld handle and overturn around the loose axle, make the mar sword be in the vertical state of mar blade down, mar blade and cell layer contact this moment promote the mar sword to remove along the mar window with the loose axle is synchronous after that, can carry out the mar processing to the cell. In the mar in-process, on the one hand, the mar window has the mar guide effect, has ensured the straightness accuracy of mar, and on the other hand, mar blade width is certain to make the mar width fixed, wound edge neat, the mar is showing and is being superior to traditional rifle head mar effect, makes the mar data more accurate reliable, also is favorable to subsequent observation, the analysis of shooing.

Finally, although the present invention has been described in detail with reference to the preferred embodiments, it should be understood by those skilled in the art that the present invention can be modified or replaced by other means without departing from the spirit and scope of the present invention, which should be construed as limited only by the appended claims.

Claims

1. A simple and stable cell mar device which characterized in that: include and match the mar lid that the lid connects with the cell culture board, the mar covers and sets up many and cell culture board go up the cell culture hole position one-to-one and along mar lid thickness direction and run through the mar window that the mar was covered, and every mar window department all is provided with the mar sword through the loose axle, loose axle and mar window sliding fit, the loose axle pass the mar sword and with mar sword normal running fit.

2. The handy and stable cell streaking apparatus as set forth in claim 1, wherein: the inner walls of the two sides of each scratch window are provided with sliding grooves which are respectively in sliding fit with the two ends of the corresponding movable shafts.

3. The handy and stable cell streaking apparatus as set forth in claim 2, wherein: every mar window all is parallel with the width direction of mar lid, and the direction that the mar window extends is unanimous with the direction of cell culture hole diameter.

4. The handy stable cell streaking apparatus as set forth in any one of claims 1 to 3 wherein: the scratching knife comprises a handheld handle, a connecting handle and a scratching blade which are sequentially connected from top to bottom.

5. The handy and stable cell streaking apparatus as set forth in claim 4, wherein: the movable shaft passes through the joint of the handheld handle and the connecting handle, and the total length of the connecting handle and the scratching blade is equal to the vertical length between the movable shaft and the bottom of the cell culture hole.

6. The handy and stable cell streaking apparatus as set forth in claim 5, wherein: the width of the scoring blade tip is 0.4-0.6 mm.