CN211570644U - Novel cell mar experiment device - Google Patents

Abstract

The utility model discloses a novel cell mar experiment device. The device comprises a culture plate cover, wherein a culture hole is formed in the culture plate cover, and the culture plate cover consists of a plate cover outer layer and a plate cover inner layer; the two sliding rails are respectively arranged on two sides of the culture plate cover; two ends of one sliding rod are respectively arranged on the two sliding rails in a sliding manner; a plurality of scratch latex sheets are mounted on the slide bar. Wherein, form mar location chamber between the outer layer of plate cover and the plate cover inlayer, and mar emulsion piece stretches into perpendicularly in the mar location chamber. The utility model provides a novel cell mar experimental apparatus is through using integration culture plate lid and mar location chamber isotructure, and the pollution source of cell top when reducing the mar has avoided the crooked distortion of mar, guarantees the mar uniformity to reduce operating error, improved experiment repeatability, avoided the harmful factor that influences the experiment.

Description

Novel cell mar experiment device

Technical Field

The utility model relates to a biological assay equipment field, in particular to novel cell mar experiment device.

Background

The wound healing (cell scratch) experiment is an experiment to study the ability of cells to migrate in vitro. The principle is as follows: when the cells grow to be fused into a single-layer state, a blank cell-free area is artificially manufactured on the fused single-layer cells, namely the scratch area, the cells at the edge of the scratch area gradually enter the blank area to heal the scratch, then the migration capability of the cells can be judged by observing the cells at the edge of the cell-free area in different periods, the cells at the edges can start migration activity, even the whole cell-free area can be covered, and the cells are contacted with each other again to achieve the healing effect. Wound healing experiments are a simple, inexpensive method and one of the first developed methods to study the migration of committed cells in vitro. This approach mimics the migration process of cells during healing in vivo.

In the scheme of the existing wound healing experiment, after cells are attached to the wall in a culture dish, a gun head of a liquid-transferring gun is generally manually operated to scratch in the middle of the attached cells, so that a wound (scratch) is artificially generated, the width of the scratch is measured at regular time, and then cell migration data for wound healing are obtained. This solution has several disadvantages:

1. scratching is carried out above the cells by using the pipette tip, so that the contact time of the cells and the outside is longer, and the probability of polluting the cells is high;

2. when the scratch is manually performed, the scratch is performed by using a straight ruler or a pipette tip only by hand feeling, so that the consistency of scratch positions among multiple holes cannot be ensured, the weight of the lower hand is not easy to control, the scratched marks are not uniform, even skew and distortion are easily caused, and the repeatability of an experiment is poor;

3. when the scratch is made, a piece of cells can be scraped at the same time, so that the cells at the edge of the scratch are mechanically damaged;

4. if the coating protein exists at the bottom of the culture dish, when the gun head is scratched, the coating layer is likely to be damaged, and then the cell migration is influenced, so that the determining influence of the factors is difficult to judge in the experimental result;

5. the width of the scratch needs to be measured manually at regular time, and the change of the scratch width along with the time is calculated, so that when a scratch measuring point is selected, subjective errors (points with migration results meeting experimental expectations are selected manually) are inevitably introduced.

SUMMERY OF THE UTILITY MODEL

In order to solve the problem, the utility model provides a novel cell mar experiment device.

According to one aspect of the utility model, a novel cell scratch experimental device is provided, which comprises a culture plate cover, wherein the culture plate cover is provided with culture holes and consists of a plate cover outer layer and a plate cover inner layer;

the two sliding rails are respectively arranged on two sides of the culture plate cover;

the two ends of the sliding rod are respectively installed on the two sliding rails in a sliding mode;

and the scratch latex sheets are installed on the sliding rod.

Wherein, form mar location chamber between the board lid skin with the board lid inlayer, and mar emulsion piece stretches into perpendicularly in the mar location chamber.

The novel cell scratch experimental device reduces the pollution source above cells during scratching by using the integrated culture plate cover and scratch positioning cavity and other structures, avoids the distortion of scratches, and ensures the consistency of scratches, thereby reducing the operation error and improving the experimental repeatability; meanwhile, the scratch latex sheet is used for reducing the mechanical damage of cells and reducing the possibility of damaging the protein bag at the bottom of the culture plate, thereby avoiding adverse factors influencing the experiment as much as possible.

In some embodiments, be formed with many mar location dead slots in the mar location chamber, mar location dead slot passes culture hole and with the slip track is parallel, the mar emulsion piece stretches into perpendicularly in the mar location dead slot. From this, through setting up the removal orbit that mar location dead slot has restricted mar emulsion piece, improved the accuracy of mar.

In some embodiments, the number of score locating voids is 4 or 6. Thereby, the number of the scratch positioning empty grooves is set, wherein, in actual operation, appropriate setting can be performed according to specific situations.

In some embodiments, the number of culture wells is 6, arranged in 2 rows and 3 columns. Thus, the plate cover can be set to a more common six-well plate.

In some embodiments, two of the score alignment cavities are disposed through each of the culture wells. From this, set up the relation of mar location dead slot and cultivation hole, and the quantity of mar location dead slot can be decided according to the concrete mode of placing of culture plate lid.

In some embodiments, the number of culture wells is 1. Thus, only one culture well can be provided on the plate cover as needed.

In some embodiments, both ends of the sliding rail are formed with placing grooves. Thereby, the placement groove can be used for placing the slide bar.

In some embodiments, two ends of the sliding rod are movably mounted on the two sliding tracks respectively, and the sliding rod can rotate around the central axis of the sliding rod. Therefore, when the scratch-proof rubber sheet does not slide, the rotating sliding rod can be used for rotating the scratch latex sheet to the position above the scratch positioning cavity so as to prevent the scratch latex sheet from contacting cells to cause unnecessary scratches.

In some embodiments, at least one end of the sliding bar is mounted on the sliding rail through an articulation detent, and the sliding bar can rotate on a vertical plane centered on the articulation detent. From this, after the mar is accomplished, can rotate the top with the slide bar and slide again and can the normal position, can avoid the mar emulsion piece of installation above that to contact the cell to cause unnecessary mar.

In some embodiments, the scratch latex sheet has a thickness of 0.5mm to 2.0 mm. Thus, the specific thickness of the scratch latex sheet is set, and the values such as 0.5mm, 1.0mm, 1.5mm, and 2.0mm are more preferable.

Drawings

Fig. 1 is a top view of an internal structure of a novel cell scratch experimental apparatus according to an embodiment of the present invention;

FIG. 2 is a side view of the novel cell scratch testing apparatus shown in FIG. 1;

FIG. 3 is a top view of the internal structure of another embodiment of the novel cell scratch testing apparatus shown in FIG. 1;

fig. 4 is a plan view of the internal structure of another novel cell scratch testing apparatus according to an embodiment of the present invention.

In the figure: the culture plate lid 1, the slip track 2, the slide bar 3, mar emulsion piece 4, cultivates hole 11, and the plate lid is outer 12, plate lid inlayer 13, mar location chamber 14, mar location dead slot 15, standing groove 21, swing joint screens 31.

Detailed Description

The present invention will be described in further detail with reference to the accompanying drawings.

Fig. 1 schematically shows a top view of an internal structure of a novel cell scratch experimental apparatus according to an embodiment of the present invention, and fig. 2 shows a side view of the novel cell scratch experimental apparatus of fig. 1; FIG. 3 is a top view of the internal structure of another embodiment of the novel cell scratch testing apparatus of FIG. 1. As shown in FIGS. 1 to 3, the apparatus comprises a plate cover 1, and a plurality of culture wells 11 are provided in the plate cover 1. The culture plate cover 1 is composed of a plate cover outer layer 12 and a plate cover inner layer 13, wherein the plate cover outer layer 12 is positioned on the outer side of the plate cover inner layer 13, and a scratch positioning cavity 14 is formed between the plate cover outer layer 12 and the plate cover inner layer 13. In addition, a plurality of scratch positioning cavities 15 are formed in parallel in the scratch positioning chamber 14, and each scratch positioning cavity 15 passes through the culture well 11 and corresponds to a locus of a position on which a scratch is required.

Preferably, the plate cover 1 generally uses a six-well plate, i.e., six culture wells 11 are provided thereon, and is arranged in 2 rows and 3 columns. Further, two scoring positioning recesses 15 are generally provided for each culture well 11, and therefore, the number of positioning recesses can be selected to be 4 or 6 according to the two different orientations of FIG. 1 and FIG. 3. Further, it is even conceivable to provide the scribing positioning cavity 15 and the corresponding scribing means in both directions simultaneously to perform scribing.

Two parallel sliding rails 2 are respectively arranged on two sides of the culture plate cover 1, and a sliding rod 3 is arranged on the two sliding rails 2, namely two ends of the sliding rod 3 are respectively arranged on the two sliding rails 2 and can slide along the sliding rails 2. The sliding rail 2 is generally parallel to the scratch positioning hollow slot 15, and the sliding rod 3 is perpendicular to the sliding rail 2. Further, a slightly concave placing groove 21 is formed at both ends of the slide rail 2 for placing the slide bar 3 at rest.

A plurality of mar emulsion pieces 4 are installed in proper order to distribute on slide bar 3, and each mar emulsion piece 4 can perpendicular downwardly extending and stretch into each mar location dead slot 15 in mar location chamber 14 to be arranged in carrying out the mar to the cell in culturing hole 11. The thickness of the scratch latex sheet 4 is generally 0.5-2.0 mm, and preferably 0.5mm, 1.0mm, 1.5mm, 2.0mm and the like.

Preferably, the two ends of the sliding rod 3 are movably mounted on the two sliding rails 2 respectively, and the movable mounting means that the sliding rod 3 can rotate around the central axis thereof without being hindered by the sliding rails 2. The scratch latex sheet 4 on the slide bar 3 can be driven to rotate together by the rotation of the slide bar, wherein when the scratch latex sheet 4 rotates vertically downwards, the scratch latex sheet can be inserted into the scratch positioning empty groove 15 and scratches cells in the culture hole 11; and when the scratch latex sheet 4 rotates to the horizontal direction and even upwards, the scratch latex sheet can be separated from the scratch positioning empty groove 15, and the interference on the cells in the culture hole 11 is avoided.

Preferably, at least one end of the slide bar 3 is installed on the slide rail 2 through a movable connection position 31, and both ends of the slide bar 3 can be installed through the movable connection position 31, and after the installation, the slide bar 3 can rotate on a vertical plane by taking the movable connection position 31 as a center, so as to be far away from the scratch positioning empty groove 15.

When using this cell mar experimental apparatus, transfer the cell to culture in culture hole 11 and cultivate, when needs carry out the mar, make slide bar 3 slide from one end to the other end along slide rail 2, the state that scratch emulsion piece 4 installation on it keeps extending perpendicularly to each scratch location dead slot 15, can carry out the mar to the cell in culture hole 11 in the slip.

After one scratch is finished, slide bar 3 may be rotated to raise scratch latex sheet 4 out of scratch locating cavity 15, and slide bar 3 may then be slid back. Wherein, one end of the sliding rod 3 can also rotate to the upper part by taking the movable connection clamping position 31 as the center so as to be further far away from the culture hole 11, thereby avoiding the interference to the cells therein.

In addition, can also only set up one to two mar emulsion pieces 4 on slide bar 3 to make mar emulsion piece 4 can slide along slide bar 3, then can be under the condition of scratch emulsion piece 4 different positions on slide bar 3 carry out above-mentioned process flow repeatedly, in order to realize carrying out the mar work in proper order to each mar location dead slot 15 or each cultivation hole 11.

Fig. 4 schematically shows a top view of the internal structure of a novel cell scratch experimental apparatus according to an embodiment of the present invention. As shown in FIG. 4, only one plate-shaped culture well 11 may be provided in the plate cover 1 as required, and all the score line positioning recesses 15 are formed through the plate-shaped culture well 11, and the structure of the other parts is substantially the same as that of the embodiment in principle.

What has been described above are only some embodiments of the invention. For those skilled in the art, without departing from the inventive concept, several modifications and improvements can be made, which are within the scope of the invention.

Claims (10)

1. The utility model provides a novel cell mar experiment device which characterized in that: comprises that

A plate cover (1), wherein the plate cover (1) is provided with culture holes (11), and the plate cover (1) is composed of a plate cover outer layer (12) and a plate cover inner layer (13);

the two sliding rails (2) are respectively arranged on two sides of the culture plate cover (1);

the two ends of the sliding rod (3) are respectively installed on the two sliding rails (2) in a sliding mode;

the scratch latex sheets (4) are mounted on the sliding rod (3);

wherein, form mar location chamber (14) between board lid skin (12) and board lid inlayer (13), and mar emulsion piece (4) stretch into perpendicularly in mar location chamber (14).

2. The novel cell scratch experiment device according to claim 1, characterized in that: be formed with many mar location dead slots (15) in mar location chamber (14), mar location dead slot (15) are passed cultivate hole (11) and with sliding rail (2) parallel, mar emulsion piece (4) stretch into perpendicularly in mar location dead slot (15).

3. The novel cell scratch experiment device according to claim 2, characterized in that: the number of the scratch positioning empty grooves (15) is 4 or 6.

4. The novel cell scratch experiment device according to claim 3, characterized in that: the number of the culture holes (11) is 6, and the culture holes are arranged in 2 rows and 3 columns.

5. The novel cell scratch experiment device according to claim 4, characterized in that: each culture hole (11) passes through two scratch positioning empty grooves (15).

6. The novel cell scratch experiment device according to claim 3, characterized in that: the number of the culture holes (11) is 1.

7. The novel cell scratch experiment device according to claim 1, characterized in that: both ends of the sliding rail (2) are provided with placing grooves (21).

8. The novel cell scratch experiment device according to claim 1, characterized in that: two ends of the sliding rod (3) are movably mounted on the two sliding tracks (2) respectively, and the sliding rod (3) can rotate around a central axis of the sliding rod.

9. The novel cell scratch experiment device according to claim 1, characterized in that: at least one end of the sliding rod (3) is installed on the sliding track (2) through a movable connection clamping position (31), and the sliding rod (3) can rotate on a vertical plane by taking the movable connection clamping position (31) as a center.

10. The novel cell scratch experiment device according to claim 9, characterized in that: the thickness of the scratch latex sheet (4) is 0.5 mm-2.0 mm.

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