CN217829950U

CN217829950U - Gel preparation device

Info

Publication number: CN217829950U
Application number: CN202220855225.5U
Authority: CN
Inventors: 张梓橦; 李磊
Original assignee: Individual
Current assignee: Individual
Priority date: 2022-04-14
Filing date: 2022-04-14
Publication date: 2022-11-18
Anticipated expiration: 2032-04-14

Abstract

The utility model discloses a gel preparation device, belonging to the field of biological experiment instruments, comprising a preparation container, a pressing plate and a plurality of locating parts; the preparation container comprises a bottom plate and a side wall enclosure fixed on the bottom plate; the pressing plate is in clearance fit with the side wall enclosure, the pressing plate can slide up and down in the side wall enclosure, and a liquid injection port is formed in the pressing plate; a plurality of locating parts are fixed at the inboard that the lateral wall encloses, and the clamp plate descends to minimum height when propping with the locating part. The utility model discloses a gel preparation facilities has set up the clamp plate, can help detach the bubble of gel, block during external pollutant gets into the gel, gel liquid spills by a large scale when can also relatively bumping, makes things convenient for the laboratory technician to confirm the volume of the gel liquid of pouring into.

Description

Gel preparation device

Technical Field

The utility model belongs to biological assay apparatus field, concretely relates to gel preparation facilities.

Background

Electrophoresis is a method of electrophoresing a sample (protein, nucleotide, etc.) with charges in an inert supporting medium under the action of an electric field toward its corresponding electrode at respective speeds to separate the components into narrow zones, and recording the electrophoretic zone pattern or calculating the percentage content thereof by a suitable detection method.

In biological laboratories, electrophoresis, particularly gel electrophoresis, is commonly used to separate, analyze or purify macromolecular substances such as proteins or nucleotides. Namely, firstly preparing gel, and then injecting macromolecular substances to be analyzed into the gel for analysis.

Gels are typically prepared by pouring a gel solution into a container, which is waiting for solidification. However, in the actual preparation process, due to the high viscosity of the gel, some air is entrained during the pouring of the gel liquid into the container, which results in the formation of a large number of visible and invisible bubbles in the gel, which can interfere with the use of the gel. The methods for removing bubbles in the prior art are troublesome and are not simple enough.

SUMMERY OF THE UTILITY MODEL

The utility model provides a gel preparation facilities aims at solving and gets rid of bubble complex operation, complicated problem in the gel among the prior art.

The utility model adopts the following technical scheme:

a gel preparation device comprises a preparation container, a pressing plate and a plurality of limiting pieces;

the preparation container comprises a bottom plate and a side wall enclosure fixed on the bottom plate; the pressing plate is in clearance fit with the side wall enclosure, the pressing plate can slide up and down in the side wall enclosure, and a liquid injection port is formed in the pressing plate;

the limiting parts are fixed on the inner sides of the side wall enclosures, and the pressing plate is abutted against the limiting parts and then is lowered to the lowest height.

In some embodiments, the preparation container further comprises a plurality of partition plates, and the partition plates divide the preparation container into a plurality of mutually separated glue containing grooves. Multiple pieces of gel can be prepared simultaneously in one gel preparation apparatus.

In some embodiments, the partition board includes a fixed partition board and a detachable partition board, the fixed partition board is fixedly connected with the bottom board and the side wall enclosure, and a slot for inserting the detachable partition board is provided on the fixed partition board and/or the side wall enclosure. The partition plate can be adjusted according to different requirements of the gel size in practical experiments, and the gel with proper size can be obtained.

In some embodiments, the number of the pressing plates is several, and each pressing plate corresponds to the shape of each glue containing groove one to one.

In some embodiments, the fixed partition is provided with the limiting member.

In some embodiments, the comb further comprises a comb body with tooth-shaped protrusions, the bottom plate is provided with a plurality of first open slots, the comb body is detachably inserted into the first open slots, and the bottom of the comb body is provided with a blocking piece which blocks the first open slots after being inserted into the first open slots. The dentate bulge of the comb body forms a sample hole of gel electrophoresis, and is used for accommodating samples such as protein or nucleotide.

In some embodiments, the bottom plate is provided with a plurality of second slots, the cutting blade can be inserted into the second slots, and the baffle can block the second slots. It will be appreciated that in the earlier preparation of the gel, the cutting blade need not be mounted on the gel preparation apparatus, but a baffle may be inserted to ensure that the gel is prepared flat. After the experiment is completed, if the gel needs to be cut to obtain the target molecule fragment, the cutting piece can be inserted for cutting the gel.

In some embodiments, the bottom plate is detachably fixed to the side wall enclosure, a transverse groove is formed in the lower end of the side wall enclosure, and the bottom plate is tightly inserted into the transverse groove. Through the close fit of the transverse groove and the bottom plate, no leakage of liquid is realized during gel preparation, and the bottom plate is convenient to draw out after the preparation is finished so as to obtain complete gel.

In some embodiments, a handle is provided on the pressure plate.

Compared with the prior art, the beneficial effects of the utility model are that:

1. the utility model discloses in set up the clamp plate, when preparing the gel, the clamp plate is installed in the top of locating part, injects the gel liquid into the container through annotating the liquid mouth on the clamp plate, and the liquid level of control gel liquid reaches the height of clamp plate, before the gel liquid is not solidified, shakes the pressure to the gel liquid through activity clamp plate from top to bottom, thereby makes the bubble in the gel liquid extruded out, discharges from the clearance that clamp plate and lateral wall enclose, has greatly reduced the bubble after the gel shaping;

2. the pressing plate is provided with the liquid injection port, the opening of the container is blocked by the pressing plate, and liquid injection can be carried out only through the liquid injection port, so that the contact area between the interior of the container and the outside is reduced, pollutants such as external dust and the like can be blocked by the pressing plate, and the possibility of pollution of gel is reduced; and when the hands of a user collide with the preparation container, the pressing plate can prevent the gel liquid in the preparation container from being spilled in a large area.

3. Because the utility model discloses in set up the clamp plate, the clamp plate highly has carried out the restriction to agarose gel liquid in preparing the container, consequently, the required volume of gel liquid is fixed when the experiment, can reach the demand according to the volume of highly coming the injection really of the gel liquid level of injection to avoid gel liquid to pour into too much waste that causes, or pour into and lead to the thinner influence experimental result of gel layer too little.

Drawings

The technology of the present invention will be further described in detail with reference to the accompanying drawings and detailed description:

FIG. 1 is a schematic view of the overall structure of the gel preparation apparatus of the present invention;

FIG. 2 is an exploded view of the gel preparation apparatus of the present invention;

FIG. 3 is a schematic structural view of the gel preparation device of the present invention with the pressing plate removed;

FIG. 4 is a schematic view of a first slot and a second slot in the base plate;

FIG. 5 is a schematic structural view of a comb body;

fig. 6 is a schematic view of the connection of the bottom plate to the side wall enclosure.

Reference numerals:

1-preparing a container; 11-a base plate; 111-first grooving; 112-second grooving; 12-side wall enclosure; 121-transverse groove; 13-fixing the partition plate; 14-a removable partition; 15-slot;

2-pressing a plate; 21-liquid injection port;

3-a stop;

4-a comb body; 41-a comb plate; 42-a baffle plate;

5-baffle plate.

Detailed Description

The conception, specific structure and technical effects of the present invention will be described clearly and completely with reference to the accompanying drawings and embodiments, so as to fully understand the objects, aspects and effects of the present invention. It should be noted that the embodiments and features of the embodiments in the present application may be combined with each other without conflict. The same reference numbers will be used throughout the drawings to refer to the same or like parts.

It should be noted that, unless otherwise specified, when a feature is referred to as being "fixed" or "connected" to another feature, it may be directly fixed or connected to the other feature or indirectly fixed or connected to the other feature. Furthermore, the description of the upper, lower, left, right, etc. used in the present invention is only relative to the mutual positional relationship of the components of the present invention in the drawings.

Referring to fig. 1 to 6, a gel preparation apparatus includes a preparation container 1, a pressing plate 2, and a plurality of stoppers 3.

Referring to fig. 1 to 4, the preparation container 1 is a rectangular container, the preparation container 1 includes a bottom plate 11 and a side wall enclosure 12 fixed on the bottom plate 11, the side wall enclosure 12 specifically includes a left side plate, a right side plate, a front panel and a rear panel, and a top view of the four connected panels is rectangular.

Referring to fig. 1 and 2, the pressing plate 2 is in clearance fit with the side wall enclosure 12, the pressing plate 2 can slide up and down in the side wall enclosure 12, so that bubbles can be pressed out through the up and down sliding, and the pressed air is discharged through a gap between the pressing plate 2 and the side wall enclosure 12. In order to facilitate the injection of the gel solution into the preparation vessel 1 through the pressure plate 2, the pressure plate 2 is provided with an injection port 21, and the gel solution is directly injected through the injection port 21. In addition, the baffle 5 also plays a role of shielding the upper end opening of the preparation container 1, and can prevent external pollutants such as dust from falling into the gel solution to cause pollution.

Referring to fig. 3, the plurality of limiting members 3 are fixed on the inner side of the sidewall 12, and can stop the pressing plate 2 from continuously descending, so that the pressing plate 2 is lowered to the lowest height when abutting against the limiting members 3, and the pressing plate 2 is prevented from being too low to sink into the gel liquid after being pressed down. Specifically, the limiting member 3 is a stopper fixed on the sidewall 12 to stop the pressing plate 2 from moving downward, and an upper end surface of the stopper is flat to support the pressing plate 2 to avoid an excessive pressure. The stop element 3 can be fixed to the side wall 12 by gluing or bolting, preferably gluing. Generally, at least two limiting members 3 are required to support one pressing plate 2.

Referring to fig. 1 and 2, the pressing plate 2 may be a transparent glass plate or acrylic plate, and bubbles below the pressing plate 2 may be directly observed through the pressing plate, so as to apply pressure in a targeted manner. In order to lift the pressing plate 2 upward, a handle (not shown) is provided on the pressing plate 2, and the pressing plate 2 can be lifted upward by grasping the handle. The bottom plate 11 and the side wall 12 may be made of glass or acrylic.

In addition, referring to fig. 2 and 3, in order to divide the prepared gel conveniently, the preparation container 1 further includes a plurality of partition plates, the partition plates divide the interior of the preparation container 1 into a plurality of mutually separated gel containing grooves, that is, the space in the container is directly divided to form mutually independent containing cavities, and when preparing the gel, gel liquid is injected into each gel containing groove respectively.

Specifically, referring to fig. 2 and 3, the partitions may be fixed, non-removable, or removable, and the gel may be selectively partitioned or non-partitioned as desired. In one embodiment, the partition comprises a fixed partition 13 and a detachable partition 14, the fixed partition 13 is fixedly connected with the bottom plate 11 and the side wall 12, and the fixed partition 13 and/or the side wall 12 is provided with a slot 15 for inserting the detachable partition 14, in this embodiment, the fixed partition 13 divides the cavity of the whole preparation container 1 into a left and a right equal parts, and the detachable partition 14 is inserted into the cavity on the right side to divide the cavity on the right side into two equal parts, i.e. the cavity of the whole preparation container 1 is divided into 1/2, 1/4 and 1/4.

Correspondingly, referring to fig. 2 and 3, after the cavity is divided into a plurality of glue receiving grooves, the pressing plate 2 also needs to be correspondingly arranged. The pressing plates 2 are provided with a plurality of pressing plates, and each pressing plate 2 is in one-to-one correspondence with the shape of each glue containing groove, so that the corresponding glue containing grooves can be respectively extruded with bubbles. It is understood that a limiting member 3 may be disposed on the fixed partition 13 to limit the pressure plate 2.

In the preparation of gel, refer to fig. 3 to 5, need form individual sample hole in the gel, for this reason, the utility model discloses a gel preparation device still includes comb body 4, and this bottom plate 11 is equipped with a plurality of first flutings 111, and this comb body 4 detachably cartridge is equipped with comb plate 41 on this comb body 4 on this first fluting 111, has the broach of individual even interval on comb plate 41, and the bottom of this comb body 4 is equipped with the separation blade 42 of this first fluting 111 of shutoff after inserting this first fluting 111. That is, the comb body 4 can be inserted into the bottom wall, and the blocking piece 42 of the comb body 4 is inserted to block the first slot 111 of the bottom plate 11, so that the leakage of the gel liquid can be avoided. After the gel is solidified and molded, the preparation vessel 1 may be turned upside down and the comb body 4 may be pulled out from the bottom plate 11, thereby forming the respective sample holes in the gel. It can be understood that the comb body 4 can be provided with various models, and the thickness and the interval size of the comb teeth on the comb body 4 with different models are different, so that different experimental requirements can be met. In one embodiment, the first slot 111 is a stepped slot, and the edge of the blocking piece 42 is also stepped so as to be inserted into the first slot 111. Alternatively, another comb plate 41 may be placed in the preparation container 1 after the first open groove 111 is closed with a plate.

Preferably, referring to fig. 2 and 3, in order to facilitate recovery of the gel, the gel preparation apparatus further comprises a cutting blade and a baffle 5, the bottom plate 11 is provided with a plurality of second slots 112, the cutting blade can be inserted into the second slots 112, and the baffle 5 can block the second slots 112. In the process of preparing the gel and the experiment, the second open slot 112 is blocked by the baffle 5, after the experiment is finished, the baffle 5 can be taken down, and the cutting piece is inserted into the second open slot 112 to cut the gel. In particular, the cutting blade is a blade. In one embodiment, the second slot 112 is a stepped slot, and the edge of the baffle 5 is also stepped so as to be inserted into the second slot 112. In one embodiment, the first slot 111 and the second slot 112 have the same pattern and size, and the baffle 5 can also be used to close the first slot 111. It can be understood that the bottom plate 11 needs to be formed with the first slot 111 and the second slot 112, and the thickness of the bottom plate 11 needs to be increased accordingly.

Referring to fig. 6, in one embodiment, the bottom plate 11 is detachably fixed to the sidewall 12, the lower end of the sidewall 12 is provided with a transverse slot 121, and the bottom plate 11 is tightly inserted into the transverse slot 121. That is, after the gel preparation is completed, the entire base plate 11 can be directly pulled out, and the sample injection can be performed to perform the experiment. The bottom plate 11 is tightly inserted into the lateral groove 121 to prevent leakage.

Other contents of the gel preparation device of the present invention are referred to in the prior art and will not be described herein.

The above description is only a preferred embodiment of the present invention, and is not intended to limit the present invention in any way, so that any modification, equivalent change and modification made by the technical spirit of the present invention to the above embodiments do not depart from the technical solution of the present invention, and still fall within the scope of the technical solution of the present invention.

Claims

1. A gel preparation device is characterized by comprising a preparation container, a pressing plate and a plurality of limiting parts;

the limiting pieces are fixed on the inner sides of the side wall enclosures, and the pressing plate is reduced to the lowest height when being abutted against the limiting pieces;

the preparation container also comprises a plurality of partition plates, and the partition plates divide the interior of the preparation container into a plurality of mutually separated glue accommodating grooves;

the baffle includes fixed baffle and removable baffle, fixed baffle with the bottom plate the lateral wall encloses fixed connection, fixed baffle and/or be equipped with on the lateral wall encloses the confession removable baffle male slot.

2. The gel preparation apparatus of claim 1, wherein the plurality of pressing plates are provided, and each pressing plate corresponds to the shape of each glue containing groove in a one-to-one manner.

3. The gel preparation apparatus of claim 1, wherein said retaining plate is provided with said stop.

4. The gel preparation device of claim 1, further comprising a comb body having teeth-like protrusions, wherein the base plate has a plurality of first slots, the comb body is detachably inserted into the first slots, and a stopper is provided at the bottom of the comb body to block the first slots after being inserted into the first slots.

5. The gel preparation device of claim 1, further comprising a cutting blade and a blocking plate, wherein the bottom plate is provided with a plurality of second slots, the cutting blade can be inserted into the second slots, and the blocking plate can block the second slots.

6. The gel preparation apparatus of claim 1, wherein the bottom plate is detachably fixed to the side wall enclosure, a transverse groove is formed at a lower end of the side wall enclosure, and the bottom plate is tightly inserted into the transverse groove.

7. A gel preparation device as claimed in claim 1 wherein a handle is provided on the pressure plate.